DE2517785A1 - Verfahren zur verbesserung der endotoxinempfindlichkeit von lumulus- lysat - Google Patents
Verfahren zur verbesserung der endotoxinempfindlichkeit von lumulus- lysatInfo
- Publication number
- DE2517785A1 DE2517785A1 DE19752517785 DE2517785A DE2517785A1 DE 2517785 A1 DE2517785 A1 DE 2517785A1 DE 19752517785 DE19752517785 DE 19752517785 DE 2517785 A DE2517785 A DE 2517785A DE 2517785 A1 DE2517785 A1 DE 2517785A1
- Authority
- DE
- Germany
- Prior art keywords
- solution
- endotoxin
- lysate
- microns
- pore size
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002158 endotoxin Substances 0.000 title claims description 41
- 238000000034 method Methods 0.000 title claims description 20
- 230000035945 sensitivity Effects 0.000 title claims description 10
- 239000006166 lysate Substances 0.000 title description 30
- 241000239218 Limulus Species 0.000 claims description 22
- 239000011148 porous material Substances 0.000 claims description 16
- 239000007787 solid Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 9
- 210000000601 blood cell Anatomy 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000010257 thawing Methods 0.000 claims description 5
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 238000011045 prefiltration Methods 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 description 51
- 238000012360 testing method Methods 0.000 description 19
- 108090000623 proteins and genes Proteins 0.000 description 18
- 102000004169 proteins and genes Human genes 0.000 description 18
- 210000004369 blood Anatomy 0.000 description 16
- 239000008280 blood Substances 0.000 description 16
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 241001529572 Chaceon affinis Species 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000023555 blood coagulation Effects 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 241000239220 Limulus polyphemus Species 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000011362 coarse particle Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000010200 folin Substances 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 239000002510 pyrogen Substances 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 101100293261 Mus musculus Naa15 gene Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000003182 parenteral nutrition solution Substances 0.000 description 1
- AVTYONGGKAJVTE-OLXYHTOASA-L potassium L-tartrate Chemical compound [K+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O AVTYONGGKAJVTE-OLXYHTOASA-L 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000001814 protein method Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US46598974A | 1974-05-01 | 1974-05-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2517785A1 true DE2517785A1 (de) | 1975-11-20 |
Family
ID=23849992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752517785 Pending DE2517785A1 (de) | 1974-05-01 | 1975-04-22 | Verfahren zur verbesserung der endotoxinempfindlichkeit von lumulus- lysat |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS50147988A (https=) |
| AU (1) | AU8069075A (https=) |
| BE (1) | BE828529A (https=) |
| DE (1) | DE2517785A1 (https=) |
| FR (1) | FR2269578B1 (https=) |
| GB (1) | GB1459733A (https=) |
| ZA (1) | ZA752378B (https=) |
-
1975
- 1975-04-15 ZA ZA00752378A patent/ZA752378B/xx unknown
- 1975-04-22 DE DE19752517785 patent/DE2517785A1/de active Pending
- 1975-04-22 GB GB1651075A patent/GB1459733A/en not_active Expired
- 1975-04-28 FR FR7513234A patent/FR2269578B1/fr not_active Expired
- 1975-04-28 JP JP50051248A patent/JPS50147988A/ja active Pending
- 1975-04-29 BE BE155897A patent/BE828529A/xx unknown
- 1975-04-30 AU AU80690/75A patent/AU8069075A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| GB1459733A (en) | 1976-12-31 |
| FR2269578B1 (https=) | 1979-01-19 |
| ZA752378B (en) | 1976-04-28 |
| BE828529A (fr) | 1975-08-18 |
| AU8069075A (en) | 1976-11-04 |
| JPS50147988A (https=) | 1975-11-27 |
| FR2269578A1 (https=) | 1975-11-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHW | Rejection |