DE19820168C2 - Verfahren zur kontinuierlichen mikrobiologischen Herstellung von Polyhydroxybuttersäure - Google Patents
Verfahren zur kontinuierlichen mikrobiologischen Herstellung von PolyhydroxybuttersäureInfo
- Publication number
- DE19820168C2 DE19820168C2 DE19820168A DE19820168A DE19820168C2 DE 19820168 C2 DE19820168 C2 DE 19820168C2 DE 19820168 A DE19820168 A DE 19820168A DE 19820168 A DE19820168 A DE 19820168A DE 19820168 C2 DE19820168 C2 DE 19820168C2
- Authority
- DE
- Germany
- Prior art keywords
- phb
- speeds
- throughput
- heat production
- jmp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 21
- 238000000034 method Methods 0.000 title claims description 19
- 239000002253 acid Substances 0.000 title claims description 7
- 230000002906 microbiologic effect Effects 0.000 title claims 2
- 239000000758 substrate Substances 0.000 claims description 26
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 25
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 11
- 239000002028 Biomass Substances 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 7
- 241000079829 Ralstonia eutropha JMP134 Species 0.000 claims description 6
- HUMNYLRZRPPJDN-UHFFFAOYSA-N benzaldehyde Chemical compound O=CC1=CC=CC=C1 HUMNYLRZRPPJDN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 241000252867 Cupriavidus metallidurans Species 0.000 claims description 4
- 241001478284 Variovorax paradoxus Species 0.000 claims description 4
- 239000005711 Benzoic acid Substances 0.000 claims description 3
- 241001478283 Variovorax Species 0.000 claims description 3
- 235000010233 benzoic acid Nutrition 0.000 claims description 3
- QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 claims description 3
- 150000002989 phenols Chemical class 0.000 claims description 3
- 230000000644 propagated effect Effects 0.000 claims description 3
- 241000589519 Comamonas Species 0.000 claims description 2
- 241000589518 Comamonas testosteroni Species 0.000 claims description 2
- 241001600125 Delftia acidovorans Species 0.000 claims description 2
- 241000232299 Ralstonia Species 0.000 claims description 2
- 238000010924 continuous production Methods 0.000 claims description 2
- 230000009036 growth inhibition Effects 0.000 claims description 2
- 238000000265 homogenisation Methods 0.000 claims 1
- 238000009423 ventilation Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 description 15
- 238000003786 synthesis reaction Methods 0.000 description 12
- 239000002609 medium Substances 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 6
- 239000004299 sodium benzoate Substances 0.000 description 6
- 235000010234 sodium benzoate Nutrition 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- -1 methanol Chemical compound 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000193033 Azohydromonas lata Species 0.000 description 2
- 241001148217 Methylobacterium rhodesianum Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000002826 coolant Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005496 tempering Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000012366 Fed-batch cultivation Methods 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 238000012365 batch cultivation Methods 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 150000001722 carbon compounds Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000019525 primary metabolic process Effects 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
- C08G63/02—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds
- C08G63/06—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds derived from hydroxycarboxylic acids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/129—Energy recovery, e.g. by cogeneration, H2recovery or pressure recovery turbines
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19820168A DE19820168C2 (de) | 1998-04-30 | 1998-04-30 | Verfahren zur kontinuierlichen mikrobiologischen Herstellung von Polyhydroxybuttersäure |
| JP2000547251A JP4416947B2 (ja) | 1998-04-30 | 1999-04-26 | ポリヒドロキシ酪酸の連続微生物生産方法 |
| AU39287/99A AU3928799A (en) | 1998-04-30 | 1999-04-26 | Method for the continuous microbiological production of polyhydroxy butyric acid |
| PCT/EP1999/002803 WO1999057298A1 (de) | 1998-04-30 | 1999-04-26 | Verfahren zur kontinuierlichen mikrobiologischen herstellung von polyhydroxybuttersäure |
| US09/787,580 US6395520B1 (en) | 1998-04-30 | 1999-04-26 | Method for the continuous microbiological production of polyhydroxy butyric acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19820168A DE19820168C2 (de) | 1998-04-30 | 1998-04-30 | Verfahren zur kontinuierlichen mikrobiologischen Herstellung von Polyhydroxybuttersäure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE19820168A1 DE19820168A1 (de) | 1999-11-04 |
| DE19820168C2 true DE19820168C2 (de) | 2001-07-12 |
Family
ID=7866812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19820168A Expired - Fee Related DE19820168C2 (de) | 1998-04-30 | 1998-04-30 | Verfahren zur kontinuierlichen mikrobiologischen Herstellung von Polyhydroxybuttersäure |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6395520B1 (enExample) |
| JP (1) | JP4416947B2 (enExample) |
| AU (1) | AU3928799A (enExample) |
| DE (1) | DE19820168C2 (enExample) |
| WO (1) | WO1999057298A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2287178T3 (es) * | 2000-12-21 | 2007-12-16 | Helmholtz-Zentrum Fur Umweltforschung Gmbh-Ufz | Procedimiento para la fabricacion biotecnologica continua de solutos compatibles a partir de sustancias toxicas. |
| KR20040046678A (ko) * | 2002-11-28 | 2004-06-05 | (주) 켐포트 | 폴리-베타-하이드록시 부틸산을 생산하는 신규의 미생물 및 그를 이용한 고농도 발효 제조방법 |
| US8735113B2 (en) | 2003-10-15 | 2014-05-27 | Newlight Technologies, Llc | Methods and systems for production of polyhydroxyalkanoate |
| US7745197B1 (en) | 2003-10-15 | 2010-06-29 | Newlight Technologies, Llc | Process for the utilization of ruminant animal methane emissions |
| US7579176B2 (en) | 2003-10-15 | 2009-08-25 | Newlight Technologies, Llc | Method for the production of polyhydroxyalkanoic acid |
| EP1924683B1 (en) * | 2005-08-22 | 2015-04-01 | Newlight Technologies, LLC | Process for the treatment of methane emissions |
| US7682789B2 (en) * | 2007-05-04 | 2010-03-23 | Ventana Medical Systems, Inc. | Method for quantifying biomolecules conjugated to a nanoparticle |
| MY180619A (en) * | 2009-06-29 | 2020-12-03 | Univ Putra Malaysia | A novel bacterium producing polyhydroxyalkanoates from palm oil mill effluent |
| US20120165500A1 (en) | 2009-08-27 | 2012-06-28 | Newlight Technologies, Llc | Process for the production of polyhydroxyalkanoates |
| US9040267B2 (en) | 2011-03-08 | 2015-05-26 | Newlight Technologies, Llc | Polyhydroxyalkanoate production method |
| US12060597B2 (en) | 2011-12-02 | 2024-08-13 | Newlight Technologies, Inc. | Polyhydroxyalkanoate production methods and systems for same |
| US20200347417A1 (en) | 2012-03-29 | 2020-11-05 | Newlight Technologies, Inc | Polyhydroxyalkanoate production methods and materials and microorganisms used in same |
| US9085784B1 (en) | 2012-03-29 | 2015-07-21 | Newlight Technologies, Llc | Polyhydroxyalkanoate production methods and materials and microorganisms used in same |
| EP2749650B1 (en) * | 2012-12-27 | 2019-03-06 | Veolia Water Solutions & Technologies Support | Method for producing polyhydroxyalkanoates by microorganisms |
| CN109265335A (zh) * | 2018-09-26 | 2019-01-25 | 东莞理工学院 | 一种利用聚3-羟基丁酸酯制备正丁酸的方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69432240T2 (de) * | 1993-09-10 | 2004-02-05 | Mitsubishi Gas Chemical Co., Inc. | Verfahren zur Herstellung von bakterien Zellen, welche poly-3-hydroxy Buttersäure enthalten |
| US5874291A (en) * | 1994-05-20 | 1999-02-23 | University Of Washington | Degradation of environmental toxins by a filamentous bacterium |
-
1998
- 1998-04-30 DE DE19820168A patent/DE19820168C2/de not_active Expired - Fee Related
-
1999
- 1999-04-26 WO PCT/EP1999/002803 patent/WO1999057298A1/de not_active Ceased
- 1999-04-26 JP JP2000547251A patent/JP4416947B2/ja not_active Expired - Fee Related
- 1999-04-26 US US09/787,580 patent/US6395520B1/en not_active Expired - Fee Related
- 1999-04-26 AU AU39287/99A patent/AU3928799A/en not_active Abandoned
Non-Patent Citations (2)
| Title |
|---|
| Biosis Abstract 1995, Nr. 360499 * |
| Biosis Abstract 1998, Nr. 122995 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999057298A1 (de) | 1999-11-11 |
| US6395520B1 (en) | 2002-05-28 |
| JP2002513584A (ja) | 2002-05-14 |
| AU3928799A (en) | 1999-11-23 |
| DE19820168A1 (de) | 1999-11-04 |
| JP4416947B2 (ja) | 2010-02-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |
Effective date: 20121101 |