DE1617500C3 - Preservation of blood plasma - Google Patents

Description

Blood plasma is made from blood by removing the red blood cells, for example by centrifugation won. To avoid premature clotting, sodium citrate is added to the blood in a known manner or sodium polyphosphate added. The remaining liquid is clear and light yellow in color. In the open air if stored, this plasma goes into putrefaction after a few days and then develops a putrid odor.

Because of this bacterial degradability, the blood plasma can become therapeutic and cosmetic Cannot be used for purposes without an antibacterial protective agent. There are only a few today Preservatives known that can be added to the blood plasma without it clotting bring. The preservative effectiveness is often only given for a few weeks, or because of the properties such means, not justifiable for certain purposes. (Formalin, oxybenzoic acids).

It has now been found that novel disinfectants which are based on peroxide and can act as wound healing agents by themselves, allowing the preservation of blood plasma without a To cause coagulation of the serum protein.

As peroxides of this quality, all compounds are to be addressed that are not worth mentioning in aqueous solution Split off amounts of hydroperoxide, are physiologically tolerable and partially or completely in water can be solved.

The peroxides have proven to be particularly suitable for the preservation of blood plasma in the ozonization of unsaturated compounds in the presence of alcohols and in general can be referred to as acetal peroxides (DE-PS 10 75 801.11 66 975.12 55 660.12 87 579.13 00 564).

There are therefore acetal peroxide solutions used as a preservative for blood plasma, which from olefinically unsaturated aliphatic and alicyclic compounds by ozonization in the presence of Alcohols have been produced at a temperature below 70 ° C.

Peroxides of this composition have now depending on the organic residue present in them, a more ⁵ or less strongly developed antibacterial property, so that the results are correspondingly different in the preservation of blood plasma. These differentiated properties are explained using the following examples.

Example 1

a) Plasma production. Animal blood is obtained in a known manner by adding citrate or phosphate buffers to avoid coagulation and the solid components (blood cells) are stabilized by centrifugation removed.

b) Ozonide production. 100 ml of olive oil is suspended with 100 ml of ethanol (96%) and by passing in Ozone-containing gases completely dissolved to saturation. The solvent is then removed in vacuo largely removed below 45 ° C. What remains is a viscous mass, the acetal peroxide in olive oil.

c) 10 ml of blood plasma (a) are now mixed with 0.1 ml Acetal peroxide (b) is added and shaken vigorously, the peroxide only partially dissolving. The plasma is now preserved and can be kept for months.

Example 2

100 ml of blood plasma (corresponding to Example 1 a) mixed with 8 ml of sorbic acid ozonide solution. This solution was prepared so that in.Ethyldiglykol 4% Sorbic acid were dissolved and then charged with ozone-containing gases until saturation.

The preparation shown in this way is not yet completely germ-free, it still contains saprophytes.

Example 3

The solution prepared according to Example 2 is made in a known manner using fluorocarbons bottled as a propellant in spray cans. The filled blood plasma remains germ-free and for years resistant.

B e i s ρ i e I 4

Proceed in the same way as in Examples 2 and 3

other acetal peroxides can also be used for preservation, as they were after DE-PS 10 75 801.11 66 975.12 55 660 12 87 579 and 13 00 564 have been described.

Claims (1)

Claim: Using Acetalperoxidlösungen, the aliphatic and alicyclic of olefinically unsaturated compounds are prepared by ozonisation in the presence of alcohols at a temperature below 7O ⁰ C, as a preservative for blood plasma.