DE148410C - - Google Patents

Description

& λ ($$ \ ι ^ι λΐΊ \\ <has
ΐ-, ί ii7ß \ ηζ lc $ -ζ- <± vmmü%:

IMPERIAL

PATENT OFFICE.

M 148410 CLASS 53 /.

form assimilable as yeast food

The purpose of the present proceedings is that in the oil seeds respectively. their press residues contained protein substances in perfect To isolate purity in order to use it both for consumer purposes and for technical purposes Purposes to make usable.

There are currently several methods, which both in principle and in the Embodiment with the present in

ίο certain relationship could be brought. According to patent specification 64354, the protein substances contained in various waste products such as blood meal, leather waste, fish guano, oil cake and the like are removed by prolonged heating with organic or inorganic acids at 3 to 4 atmospheres. Pressure peptonized and so in one for their use
transformed.

Isolation of the reaction products is not intended.

According to British patent specification 3391 of 1898, peptone-free albumoses can be obtained from any protein (including meat) by heating the relevant materials with inorganic acids, such as sulfurous anhydride, boric acid, phosphoric acid, calcium bisulfite, at 90 ^° C. in an autoclave for several hours . The albumoses obtained by evaporation are a yellow powder that is completely soluble in water.

Another method (patent specification 121439) is used to loosen the egg

white substances from oil seeds a 4 to 5 percent aqueous sodium bisulfite solution and leaves the same ι up to 2 days at sun temperature

the raw materials act. The resulting solution becomes the protein precipitated by means of an acid.

According to patent specification 124371, the oil seeds are freed from coloring, smelling and tasting substances by washing with a dilute acid that does not dissolve protein, such as sulfuric acid, nitric acid, oxalic acid, and the raw materials purified in this way are added to the protein by digestion for two hours with highly dilute hydrochloric acid 30 ⁰ C. withdrawn. The dissolved proteins are separated out by neutralization.

The present procedure is carried out as follows:

100 kg cottonseed flour are mixed with a very dilute calcium hydroxide solution containing at most 0.05 percent Ca (OH) ₂ and treated with the same for about 1 hour while stirring continuously, then ^{heated to 30 to 40 °} C. and nitrated. The residue is washed with water from 30 'to 40 ^° C. as long as a colored or. solution colored on addition of strong alkali is obtained. The raw materials cleaned and washed in this way are now exposed to the action of 500 liters of a 0.5 to ι percent solution of orthophosphoric acid in the cold with constant stirring for about 1 hour, whereupon the vessel is closed and. heated to 50 to 60 ° C for about 15 to 30 minutes.

This treatment removes all of the proteins present in the raw materials brought into solution as Acidalbumine. The mass is then filtered and removed from the obtained, opalescent, pale yellow lo-

Claims (1)

The protein is precipitated by adding sulfuric acid or nitric acid. After this When the precipitate settles, the supernatant liquid is removed and washed Protein with water as long as it is still acidic. ■ The excreted protein represents a fine white precipitate, which after washing with water and drying in ίο vacuum in the form of a gelatinous mass, after washing with alcohol as a fine, white, odorless and tasteless powder is obtained. The purification of the raw materials, a measure which is ^{absolutely necessary to achieve a pure 1} preparation, is based on the observation that the smelling, coloring and tasting substances contained in the oil seeds are in very dilute solutions of hydroxides of the alkaline earths are easily and completely soluble, while the proteins are not changed by the solvents mentioned. About the behavior of the proteins of the oil seeds against the above mentioned salt solutions is so far little known. There are only incomplete and contradicting information in this regard before; these also relate to proteins from other materials (cf. z. B. Ritthausen: The protein bodies of cereals, legumes and oil seeds, Bonn, 1872, page 49, 109/10, 123, 129 and 137). Accordingly, it could not be based on the existing literature or on the basis of the at the beginning mentioned patents, the cleaning applied in the present process can be foreseen. '■ In order to isolate the protein from the purified raw materials, the treatment turned out to be with a protein-dissolving acid, e.g. B. 0.5 to ι percent orthophosphoric acid, in warmth as the most expedient. According to the patents listed above, the solution is BEZW. Conversion · the Proteins by organic and inorganic acids in the heat and under significant Pressure. However, it must be taken into account here that according to British Patent 3391 of 1898 the extraction of albumen, d. H. protein compounds easily soluble in water, is sought, while according to the patent specification 64354 the formation of Peptones, event, also made from amido acids. The methods mentioned are hereinafter both in principle and in their embodiment essentially different from the present one. Although it is not unknown that the proteins come into contact with acids more or less depending on the concentration, temperature and duration of exposure can be quickly converted into Acidalbumine and, as such, easily dissolved by certain acids this property of proteins can already be used for technical production has been used by vegetable protein '(see patent specification 124371), so it was neither on the basis of literature references nor on the basis of the patent specifications cited possible to foresee under what circumstances the proteins of the oil seeds under Consideration of the pretreatment is best converted into Acidalbumine respectively. as such can be brought into solution, since the proteins are known to be contained therein behave very differently. The protein substances dissolved in orthophosphoric acid are separated out by means of additives a strong acid that does not dissolve protein, such as sulfuric acid or nitric acid. According to what has been said, the present method consists of a series of details of which the dissolution of proteins in acids, as well as the precipitation this solution through an acid cannot be regarded as a novelty in the strict sense of the word can; However, the combination of the individual is to be regarded as a patentable novelty Operations of the process to a set for the purpose of achieving the desired technical Effect. Pa τ ε ν τ - A ν s ρ r υ c η: Process for obtaining "protein from seeds or press residues of the oil industry, in particular from cotton seeds, characterized in that these materials are subjected to a pretreatment by means of very dilute aqueous solutions of hydroxides of the alkaline earths in order to remove coloring, smelling and tasting substances In this way the protein is removed from raw materials purified by treatment with a dilute acid, e.g. orthophosphoric acid, expediently in the warmth and under gentle pressure, and that finally the protein substances ^X1 5 from the protein solution obtained by the addition of a strong, heavy protein dissolving acid, e.g. nitric acid, sulfuric acid.