DE102015207516A1 - Kopplung von Proteinen von Interesse (POI) mit viralen Vektoren mittels Intein-vermittelten Proteinspleißens - Google Patents

Kopplung von Proteinen von Interesse (POI) mit viralen Vektoren mittels Intein-vermittelten Proteinspleißens Download PDF

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DE102015207516A1
DE102015207516A1 DE102015207516.8A DE102015207516A DE102015207516A1 DE 102015207516 A1 DE102015207516 A1 DE 102015207516A1 DE 102015207516 A DE102015207516 A DE 102015207516A DE 102015207516 A1 DE102015207516 A1 DE 102015207516A1
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protein
intein
rir1
virus
dnab
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Christian Buchholz
Alexander Muik
Anke Rasbach
Thorsten Friedel
Irene Schneider
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Paul-Ehrlich-Institut
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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DE102015207516.8A 2015-04-23 2015-04-23 Kopplung von Proteinen von Interesse (POI) mit viralen Vektoren mittels Intein-vermittelten Proteinspleißens Withdrawn DE102015207516A1 (de)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000047751A1 (en) 1999-02-12 2000-08-17 New England Biolabs, Inc. Intein-mediated protein ligation of expressed proteins
DE10215123A1 (de) 2002-04-05 2003-10-16 Klaus Cichutek Von SIVsmmPBj14 abgeleitete lentivirale Vektoren, Verfahren zu ihrer Herstellung und ihre Verwendung zur Genübertragung in Säugerzellen
WO2008037458A2 (en) 2006-09-27 2008-04-03 Bundesrepublik Deutschland, Letztvertreten Durch Den Präsidenten Des Paul-Ehrlich-Instituts Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high throughput screening
WO2008058752A2 (en) 2006-11-16 2008-05-22 Bundesrepublik Deutschland, Letztvertreten Durch Den Präsidenten Des Paul-Ehrlich-Instituts Lentiviral vectors for gene transfer in quiescent (g0) cells
WO2012117116A2 (en) 2011-03-03 2012-09-07 Bundesrepublik Deutschland, Letztvertreten Durch Den Präsidenten Des Paul-Ehrlich-Instituts, Prof. Dr. Klaus Cichutek Enhanced tumor therapy by tumor stem cell targeted oncolytic viruses
WO2013045632A1 (en) 2011-09-28 2013-04-04 Era Biotech, S.A. Split inteins and uses thereof
WO2013104728A1 (en) 2012-01-11 2013-07-18 Paul-Ehrlich-Institut Bundesamt Für Sera Und Impfstoffe Novel pseudotyped lentiviral particles and their use in the in vitro targeted transduction of undifferentiated pluripotent human embryonic stem cells and induced pluripotent stem cells

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000047751A1 (en) 1999-02-12 2000-08-17 New England Biolabs, Inc. Intein-mediated protein ligation of expressed proteins
DE10215123A1 (de) 2002-04-05 2003-10-16 Klaus Cichutek Von SIVsmmPBj14 abgeleitete lentivirale Vektoren, Verfahren zu ihrer Herstellung und ihre Verwendung zur Genübertragung in Säugerzellen
WO2008037458A2 (en) 2006-09-27 2008-04-03 Bundesrepublik Deutschland, Letztvertreten Durch Den Präsidenten Des Paul-Ehrlich-Instituts Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high throughput screening
WO2008058752A2 (en) 2006-11-16 2008-05-22 Bundesrepublik Deutschland, Letztvertreten Durch Den Präsidenten Des Paul-Ehrlich-Instituts Lentiviral vectors for gene transfer in quiescent (g0) cells
WO2012117116A2 (en) 2011-03-03 2012-09-07 Bundesrepublik Deutschland, Letztvertreten Durch Den Präsidenten Des Paul-Ehrlich-Instituts, Prof. Dr. Klaus Cichutek Enhanced tumor therapy by tumor stem cell targeted oncolytic viruses
WO2013045632A1 (en) 2011-09-28 2013-04-04 Era Biotech, S.A. Split inteins and uses thereof
WO2013104728A1 (en) 2012-01-11 2013-07-18 Paul-Ehrlich-Institut Bundesamt Für Sera Und Impfstoffe Novel pseudotyped lentiviral particles and their use in the in vitro targeted transduction of undifferentiated pluripotent human embryonic stem cells and induced pluripotent stem cells

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Title
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Anliker et al., Nat Med, 2010
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Banerjee, PS et al. (2010), J Am Chem Soc 132(39): 13615–13617
Banerjee, PS et al. (2011), J. Virol. 85(15): 7546–7554
Carvajal-Vallejos et al. (2012) (Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. J Biol Chem. 287(34): 28686–96) (insbesondere die Intein N- und Intein C-Fragmente von gp41-1, gp41-8, Nrdj-1 und IMPDH-1 von Seite 28687, Abschnitt „Experimental Procedures" und Abbildung 1; Seite 28688, Tabelle 1)
Dreier et al., (2012), Methods Mol Biol 805: 261–286
Fridy et al. (2014), Nat Methods 11: 1253–1260
Friedel et al., PEDS, 2015
Funke et al., Mol Ther, 2008
Glockshuber et al. (1992), Biochemistry 31: 1270–1279
Holliger et al. (2005), Nat Biotechnol 23: 1126–1136
InBase Datenbank (InBase, The Intein Registry; http://tools.neb.com/inbase)
InBase, The Intein Database; http://tools.neb.com/inbase/motifs_splice.php
InBase, The Intein Registry; http://tools.neb.com/inbase
Intein-Datenbank (http://tools.neb.com/inbase/)
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Perler et al. (1994) (Protein splicing elements: inteins and exteins – a definition of terms and recommended nomenclature. Nucleic Acids Res 22(7): 1125–7)
Perler et al. (1997) (Compilation and analysis of intein sequences, Nucleic Acids Res 25(6): 1087–93)
Perler et al. (1997) (Compilation and analysis of intein sequences. Nucleic Acids Res 25(6): 1087–93)
Perler, et al. (2002) (InBase: the Intein Database. Nucleic Acids Res 30(1): 383–4)
Perler, F. B. (2002); InBase, the Intein Database. Nucleic Acids Res. 30, 383–384
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Varadi et al., (2012), Gene Ther. 19: 800–809
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