DE102014012304A1 - Fermentation of whey powder solution with bacteria - Google Patents

Fermentation of whey powder solution with bacteria Download PDF

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Publication number
DE102014012304A1
DE102014012304A1 DE102014012304.9A DE102014012304A DE102014012304A1 DE 102014012304 A1 DE102014012304 A1 DE 102014012304A1 DE 102014012304 A DE102014012304 A DE 102014012304A DE 102014012304 A1 DE102014012304 A1 DE 102014012304A1
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Prior art keywords
lactic acid
whey
protein
powder
oligopeptides
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Willi Jung
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BIOGON GMBH, DE
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BIOGON GmbH
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/04—Animal proteins
    • A23J3/08—Dairy proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C21/00—Whey; Whey preparations
    • A23C21/02—Whey; Whey preparations containing, or treated with, microorganisms or enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C21/00—Whey; Whey preparations
    • A23C21/02—Whey; Whey preparations containing, or treated with, microorganisms or enzymes
    • A23C21/026—Whey; Whey preparations containing, or treated with, microorganisms or enzymes containing, or treated only with, lactic acid producing bacteria, bifidobacteria or propionic acid bacteria
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C21/00—Whey; Whey preparations
    • A23C21/06—Mixtures of whey with milk products or milk components
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
    • A23J3/343—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A23K10/28—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin from waste dairy products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/66—Proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/68—Acidifying substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/175—Amino acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A61K8/986—Milk; Derivatives thereof, e.g. butter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/10—General cosmetic use
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

Herstellung einer Lösung von Aminosäuren und Oligopeptiden in Wasser durch Fermentation von Eiweißquellen in wässriger Lösung mit oder ohne Frischmolkezusatz auf der Ausgangsbasis von in Wasser aufgelöstem Molkenpulver und/oder anderem Milchpulver und Permeatpulver und/oder auf der Basis von Gemischen der hier genannten Quellen jeglicher Art unter Zusatz von Milchsäurebakterien und/oder Proteasen und/oder Säurequellen zur Erzielung eines Milchsäurespiegels oder Säurespiegels mit pH von 4 oder kleiner, zur Spaltung und zum Abbau der vorhandenen oder zugefügten Eiweiße sowie die Verwendung dieses Compounds in flüssiger oder getrockneter Form als Grundstoff und/oder Additiv für Lebensmittel, Körperpflegeprodukte, Pharma- und Medizinprodukte sowie Tierfutter in allen Formen.Preparation of a solution of amino acids and oligopeptides in water by fermentation of protein sources in aqueous solution with or without fresh whey addition on the basis of dissolved in water whey powder and / or other milk powder and permeate powder and / or based on mixtures of the sources mentioned here under any kind Addition of lactic acid bacteria and / or proteases and / or acid sources to achieve a lactic acid level or acidity of pH 4 or smaller, for cleavage and degradation of the existing or added proteins and the use of this compound in liquid or dried form as a raw material and / or additive for food, personal care products, pharmaceutical and medical products and animal feed in all forms.

Description

Stand der TechnikState of the art

Bekannt sind die Patente DE 2819940 , DE 3924559 und EP 0850044 u. a., welche aus Molke oder Molke und Eiweiß durch Biofermentation Flüssigkeiten liefern, die breite Anwendung finden. Allen gemeinsam ist als Ausgangsbasis preiswerte Molke, sei es Süßmolke, sei es Sauermolke.The patents are known DE 2819940 . DE 3924559 and EP 0850044 Among other things, which deliver from whey or whey and protein by biofermentation liquids that are widely used. All together, the starting point is inexpensive whey, be it sweet whey or sour whey.

Nachteilig an diesen Verfahren ist der der Molke anhaftende Geruch, der sich auch auf die Folgeprodukte auswirken kann. Nachteilig ist auch, dass Bakterien, Sporen, Pilze etc. in der Molke sein können. Das Transportproblem ist zu berücksichtigen, wenn man nicht direkt am Ort des Molkeanfalls diese weiterverarbeitet.A disadvantage of this process is the odor adhering to the whey, which can also affect the secondary products. Another disadvantage is that bacteria, spores, fungi, etc. can be in the whey. The transport problem has to be taken into account if you do not process it directly at the location of the whey seizure.

Andererseits gibt es Magermilch und Permeat, beides Produkte, dem Bestandteile wie Fett und/oder Eiweiß entzogen wurde in verschiedenen Verfahren. Die Magermilch enthält neben Eiweiß noch den kompletten Milchzucker und viele bis alle wasserlöslichen Elemente (Vitamine, Mineralstoffe) der Milch.On the other hand, skimmed milk and permeate, both products that have been deprived of ingredients such as fat and / or protein, have been used in various processes. In addition to protein, skimmed milk also contains the complete milk sugar and many or all of the water-soluble elements (vitamins, minerals) in the milk.

Das Permeat enthält meist nur noch die reinen Molkeproteine und Milchzucker, kein Kasein und kein Fett, hat den Vorzug, fast geruchlos zu sein.The permeate usually contains only the pure whey protein and lactose, no casein and no fat, has the advantage of being almost odorless.

Es gibt auch UF-Permeat, ein durch Ultrafiltration total reduziertes Produkt, dem fast alle Fettanteile, Eiweißanteile, der Milchzucker und die Molkeproteine entzogen sind.There is also UF permeate, a totally reduced by ultrafiltration product, almost all fat, protein, lactose and whey proteins are removed.

Magermilch und Permeat werden in verschiedenen Formen in den Nahrungsmittelkreislauf gebracht. Als Getränk, als Pulver, als Beimischung etc.Skim milk and permeate are put into the food cycle in various forms. As a drink, as a powder, as an admixture etc.

Der Markt hat gezeigt, dass einerseits Interesse vorhanden ist, andererseits der Geruch und der Geschmack eine große Rolle spielen. Daher ist eine Standardisierung aller Produktquellen wichtig. Das geht über den Weg von Trockenpulver.The market has shown that, on the one hand, there is interest but, on the other hand, smell and taste play a major role. Therefore, standardization of all product sources is important. That goes over the way of dry powder.

Das hier angemeldete Verfahren geht von Molkenpulver als Ausgangsprodukt aus. Dies hat z. B. ca. 25% Proteine, die fast ausschließlich Molkeproteine sind. Auf Grund der Herstellung ist es praktisch keimfrei, leicht zu transportieren, gut zu lagern und sehr dosiert einzusetzen. Das Molkenpulver wird in Wasser gelöst, kann nochmals erhitzt werden zur Sterilisation und wird dann auf ca. 35 Grad C abgekühlt. Nun wird es mit Proteasen und Bakterien angeimpft, und das Ganze bei Temperaturkontrolle gären gelassen, wobei hin und wieder gerührt wird. Weitere Eiweißquellen (Quark, Trockenquark, andere Eiweißpulver und Eiweißquellen) werden zudosiert. Das so erzeugte Aminosäurengemisch ist auf Grund seines pH-Wertes von deutlich unter 4. Bevorzugt von 3,5 oder weniger sehr gut unter Luftabschluss haltbar.The process notified here starts from whey powder as starting material. This has z. B. about 25% proteins, which are almost exclusively whey proteins. Due to the production, it is practically germ-free, easy to transport, easy to store and very metered. The whey powder is dissolved in water, can be heated again for sterilization and is then cooled to about 35 degrees C. Now it is inoculated with proteases and bacteria and allowed to ferment at temperature control, stirring occasionally. Other protein sources (quark, dry quark, other protein powder and protein sources) are added. The amino acid mixture thus produced is due to its pH of well below 4. Preferably 3.5 or less very well preserved under exclusion of air.

Mittels einer anschließenden Mikrofiltration wird es geklärt, von den Bakterien befreit und man erhält eine Lösung von Aminosäuren und Oligopeptiden, in der auch noch Spurenelemente aller Art vorhanden sind. Dieses Produkt/Serum kann nun industriell verwertet werden.By means of a subsequent microfiltration, it is clarified, freed from the bacteria and gives a solution of amino acids and oligopeptides, in which even trace elements of all kinds are present. This product / serum can now be used industrially.

ZITATE ENTHALTEN IN DER BESCHREIBUNG QUOTES INCLUDE IN THE DESCRIPTION

Diese Liste der vom Anmelder aufgeführten Dokumente wurde automatisiert erzeugt und ist ausschließlich zur besseren Information des Lesers aufgenommen. Die Liste ist nicht Bestandteil der deutschen Patent- bzw. Gebrauchsmusteranmeldung. Das DPMA übernimmt keinerlei Haftung für etwaige Fehler oder Auslassungen.This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Zitierte PatentliteraturCited patent literature

  • DE 2819940 [0001] DE 2819940 [0001]
  • DE 3924559 [0001] DE 3924559 [0001]
  • EP 0850044 [0001] EP 0850044 [0001]

Claims (12)

Verfahren zur starken Anreicherung von Aminosäuren und/oder Oligopeptiden auf der Grundlage von Molkepulver, das in Wasser aufgelöst wird und dem wahlweise beliebige Mengen von Frischmolke zugesetzt werden und bei dem die vorhandenen Milchzucker- und/oder vorhandenen oder zugefügten Eiweißmengen abgebaut werden, gekennzeichnet dadurch, dass mindestens eine Protease oder ein Milchsäurebakterium den Milchzucker zu Milchsäure umwandelt und die Eiweiße zu Oligopeptiden und Aminosäuren abbaut, wobei das notwendige saure Medium durch die aus Laktose entstehende Milchsäure entsteht oder erzeugt wird durch Zufügung einer anderen Säure, einer säurebildenden Substanz und oder eines anderen Säurelieferanten.Process for the strong accumulation of amino acids and / or oligopeptides on the basis of whey powder, which is dissolved in water and to which optionally any amounts of fresh whey are added and in which the available milk sugar and / or existing or added protein amounts are broken down, characterized that at least one protease or a lactic acid bacterium converts the milk sugar to lactic acid and degrades the proteins to oligopeptides and amino acids, the necessary acidic medium being produced or produced by the lactic acid resulting from lactose by addition of another acid, an acidifying substance and / or another acid supplier , Verfahren gemäß Anspruch 1, gekennzeichnet dadurch, dass zwei Proteasen oder Milchsäurebakterien zum Einsatz kommen, wobei eine zuerst den Milchzucker zu Milchsäure umwandelt, wodurch der pH-Wert gesenkt wird, die andere bevorzugt das Eiweiß, sowohl das im Molkepulver inkludierte als auch gezielt zugefügtes, abbaut. Das zugsetzte Eiweiß kann Frischquark, Quarkpulver oder eine andere Eiweißquelle sein.A process according to claim 1, characterized in that two proteases or lactic acid bacteria are used, one first converting the milk sugar to lactic acid, thereby lowering the pH, the other preferably the protein, both included in the whey powder and deliberately added, degrades. The added protein may be fresh quark, quark powder or another source of protein. Verfahren nach Anspruch 1 und/oder 2, gekennzeichnet dadurch, dass mindestens eine Protease als Mikroorganismus vorliegt.A method according to claim 1 and / or 2, characterized in that at least one protease is present as a microorganism. Verfahren nach Anspruch 1 und/oder 2 und/oder 3, gekennzeichnet dadurch, dass der oder die Mikroorganismen aus der Familie der bekannten und auch in der Milchwirtschaft eingesetzten Milchsäurebakterien stammen.A method according to claim 1 and / or 2 and / or 3, characterized in that the microorganism or organisms come from the family of the known and used in the dairy industry lactic acid bacteria. Verfahren nach Anspruch 1 bis 4, gekennzeichnet dadurch, dass hinterher die Aminosäuren in hoher Konzentration als Monoaminosäuren und Oligopeptide mit 2–9 Aminosäureneinheiten vorliegen.A method according to claim 1 to 4, characterized in that afterwards the amino acids are present in high concentration as monoamino acids and oligopeptides with 2-9 amino acid units. Verwendung der nach den Ansprüchen 1 bis 5 hergestellten Aminosäurenmischung zur Herstellung von Lebensmittel und/oder Getränken.Use of the amino acid mixture prepared according to claims 1 to 5 for the production of food and / or drinks. Verwendung der nach den Ansprüchen 1 bis 5 hergestellten Aminosäurenmischung zur Herstellung von kosmetischen Produkten und/oder Körperpflegeprodukten und/oder Tierfutter und/oder Reinigungsprodukten und/oder Pharmastoffen.Use of the amino acid mixture prepared according to claims 1 to 5 for the production of cosmetic products and / or personal care products and / or animal feed and / or cleaning products and / or pharmaceutical substances. Verwendung der nach den Ansprüchen 1 bis 5 hergestellten Lösungen als Ausgangsstoff für ein Trockenprodukt, das selbst als Ausgangsstoff für Anwendungen nach Anspruch 6 und 7 eingesetzt wird.Use of the solutions prepared according to claims 1 to 5 as starting material for a dry product which itself is used as starting material for applications according to claims 6 and 7. Verwendung der nach Anspruch 1 bis 6 und 8 hergestellten Aminosäurenmischungen zum direkten Verkauf an Endverbraucher für deren eigene Mischungsrezepte.Use of the amino acid mixtures prepared according to claims 1 to 6 and 8 for direct sale to end consumers for their own mixture recipes. Verfahren nach den Ansprüchen 1 bis 6, dadurch gekennzeichnet, dass die Protease/n enthalten in suspendierten in vitro Zellen vorliegt/vorliegen, dass diese Zellen in ein Nährmedium inokuliert und zur Bildung eines Kulturmediums kultiviert werden.Process according to claims 1 to 6, characterized in that the protease (s) contained in suspended in vitro cells are / are present in that these cells are inoculated into a nutrient medium and cultured to form a culture medium. Verfahren nach den Ansprüchen 1–6, gekennzeichnet dadurch, dass es anaerob gehalten wird und dass die Reaktionstemperatur zwischen 20 und 40 Grad liegt, besonders bevorzugt bei 26–36 Grad C und/oder dass dabei gerührt wird.Process according to claims 1-6, characterized in that it is kept anaerobic and that the reaction temperature is between 20 and 40 degrees, more preferably at 26-36 degrees C and / or that it is stirred. Verfahren nach den Ansprüchen 1–5 und 10–11, gekennzeichnet dadurch, dass der Produkt am Ende einer speziellen Filtration unterzogen wird, besonders bevorzugt einer Umkehrosmose oder einer Mikrofiltration.Process according to claims 1-5 and 10-11, characterized in that the product is subjected at the end of a special filtration, more preferably a reverse osmosis or a microfiltration.
DE102014012304.9A 2014-08-24 2014-08-24 Fermentation of whey powder solution with bacteria Withdrawn DE102014012304A1 (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2819940B1 (en) 1978-05-06 1979-06-13 Henry Dipl-Ing Zimzik Process for the production of detergent substances based on whey using lactic acid sticks
DE3924559A1 (en) 1989-07-25 1991-05-16 Nikolaus W Prof Dr Med Klehr Use of whey in cleaning and care compsn. - for feminine hygiene, using careful control of pH
EP0850044A1 (en) 1995-09-15 1998-07-01 Advance AG Process for obtaining amino acids from proteins and use of the amino-acid-containing products obtained

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2819940B1 (en) 1978-05-06 1979-06-13 Henry Dipl-Ing Zimzik Process for the production of detergent substances based on whey using lactic acid sticks
DE3924559A1 (en) 1989-07-25 1991-05-16 Nikolaus W Prof Dr Med Klehr Use of whey in cleaning and care compsn. - for feminine hygiene, using careful control of pH
EP0850044A1 (en) 1995-09-15 1998-07-01 Advance AG Process for obtaining amino acids from proteins and use of the amino-acid-containing products obtained

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