DE102007024852A1 - Strongly enriching the amino acids and/or oligopeptides based on sweet whey powder, which is dissolved in water, comprises converting sugar to lactic acid and proteins to oligopeptides and amino acids using protease or lactic acid bacterium - Google Patents

Strongly enriching the amino acids and/or oligopeptides based on sweet whey powder, which is dissolved in water, comprises converting sugar to lactic acid and proteins to oligopeptides and amino acids using protease or lactic acid bacterium Download PDF

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DE102007024852A1
DE102007024852A1 DE102007024852A DE102007024852A DE102007024852A1 DE 102007024852 A1 DE102007024852 A1 DE 102007024852A1 DE 102007024852 A DE102007024852 A DE 102007024852A DE 102007024852 A DE102007024852 A DE 102007024852A DE 102007024852 A1 DE102007024852 A1 DE 102007024852A1
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lactic acid
amino acids
oligopeptides
acid
protease
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DE102007024852A
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German (de)
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Willi Dr. Jung
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BIOGON GmbH
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BIOGON GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C21/00—Whey; Whey preparations
    • A23C21/02—Whey; Whey preparations containing, or treated with, microorganisms or enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
    • A23J3/343—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/19—Dairy proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/20—Milk; Whey; Colostrum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/01—Hydrolysed proteins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid

Abstract

Strongly enriching the amino acids and/or oligopeptides based on sweet whey powder, which is dissolved in water, and in which the existing lactose and/or protein quantities are disintegrated, comprises converting sugar to lactic acid and proteins to oligopeptides and amino acids using at least a protease or a lactic acid bacterium, where the necessary acid medium is produced from lactic acid and also by the addition of another acid, an acid-forming substance and/or another acid supplying material.

Description

Stand der TechnikState of the art

Bekannt sind die Patente DE 2819940 , DE 3924559 und EP 0850044 u. a., welche aus Molke oder Molke und Eiweiß durch Biofermentation Flüssigkeiten liefern, die breite Anwendung finden. Allen gemeinsam ist als Ausgangsbasis preiswerte Molke, sei es Süßmolke, sei es Sauermolke.The patents are known DE 2819940 . DE 3924559 and EP 0850044 Among other things, which deliver from whey or whey and protein by biofermentation liquids that are widely used. All together, the starting point is inexpensive whey, be it sweet whey or sour whey.

Nachteilig an diesen Verfahren ist der der Molke anhaftende Geruch, der sich auch auf die Folgeprodukte auswirken kann. Nachteilig ist auch, dass Bakterien, Sporen, Pilze etc. in der Molke sein können. Das Transportproblem ist zu berücksichtigen, wenn man nicht direkt am Ort des Molkeanfalls diese weiterverarbeitet.adversely in this process is the whey adherent odor which is also affect the derived products. Another disadvantage is that bacteria, spores, fungi etc. can be in the whey. The transport problem has to be considered, if not directly at the location of the whey attack this further processed.

Andererseits gibt es Magermilch und Permeat, beides Produkte, dem Bestandteile wie Fett und/oder Eiweiß entzogen wurde in verschiedenen Verfahren. Die Magermilch enthält neben Eiweiß noch den kompletten Milchzucker und viele bis alle wasserlöslichen Elemente (Vitamine, Mineralstoffe) der Milch. Das Permeat enthält meist nur noch die reinen Molkeproteine und Milchzucker, kein Kasein und kein Fett, hat den Vorzug, fast geruchlos zu sein.on the other hand There are skim milk and permeate, both ingredients how fat and / or protein was extracted in various procedures. The skimmed milk contains in addition to protein nor the complete Lactose and many to all water-soluble elements (Vitamins, minerals) of the milk. The permeate contains usually only the pure whey proteins and lactose, no casein and no fat, has the advantage of being almost odorless.

Es gibt auch UF-Permeat, ein durch Ultrafiltration total reduziertes Produkt, dem fast alle Fettanteile, Eiweißanteile, der Milchzucker und die Molkeproteine entzogen sind.It also gives UF permeate, a totally reduced by ultrafiltration Product containing almost all fat, protein, protein Milk sugar and the whey proteins are withdrawn.

Magermilch und Permeat werden in verschiedenen Formen in den Nahrungsmittelkreislauf gebracht. Als Getränk, als Pulver, als Beimischung etc.skimmed milk and permeate are placed in various forms in the food cycle. As a drink, as a powder, as an admixture etc.

Das hier angemeldete Verfahren geht von Süßmolkenpulver als Ausgangsprodukt aus. Dies hat z. B. ca. 25% Proteine, die fast ausschließlich Molkeproteine sind. Auf Grund der Herstellung ist es praktisch keimfrei, leicht zu transportieren, gut zu lagern und sehr dosiert einzusetzen. Das Süßmolkenpulver wird in Wasser gelöst, kann nochmals erhitzt werden zur Sterilisation und wird dann auf ca. 35% abgekühlt. Nun wird es mit Proteasen und Bakterien angeimpft, und das Ganze bei Temperaturkontrolle gären gelassen, wobei hin und wieder gerührt wird. Das so erzeugte Aminosäurengemisch ist auf Grund seines pH-Wertes von meist unter 4 bis 3 sehr gut unter Luftabschluss haltbar. Mittels einer anschließenden Mikrofiltration wird es geklärt, von den Bakterien befreit und man erhält eine Lösung von Aminosäuren und Oligopeptiden, in der auch noch Spurenelemente aller Art vorhanden sind. Dieses Produkt kann nun industriell verwertet werden.The Processes notified here are of sweet whey powder as starting product. This has z. B. about 25% proteins, almost exclusively whey proteins. Due to the production It is practically germ-free, easy to transport, easy to store and to be used very dosed. The sweet whey powder is dissolved in water, can be reheated for sterilization and then cooled to about 35%. Now it will be with proteases and bacteria inoculated, and fermented at temperature control, every now and then stirring. The amino acid mixture thus produced is very good due to its pH of mostly below 4 to 3 durable under exclusion of air. By means of a subsequent Microfiltration clarifies it, removes it from the bacteria and a solution of amino acids is obtained and oligopeptides, in which there are also trace elements of all kinds are. This product can now be used industrially.

ZITATE ENTHALTEN IN DER BESCHREIBUNGQUOTES INCLUDE IN THE DESCRIPTION

Diese Liste der vom Anmelder aufgeführten Dokumente wurde automatisiert erzeugt und ist ausschließlich zur besseren Information des Lesers aufgenommen. Die Liste ist nicht Bestandteil der deutschen Patent- bzw. Gebrauchsmusteranmeldung. Das DPMA übernimmt keinerlei Haftung für etwaige Fehler oder Auslassungen.This list The documents listed by the applicant have been automated generated and is solely for better information recorded by the reader. The list is not part of the German Patent or utility model application. The DPMA takes over no liability for any errors or omissions.

Zitierte PatentliteraturCited patent literature

  • - DE 2819940 [0001] - DE 2819940 [0001]
  • - DE 3924559 [0001] - DE 3924559 [0001]
  • - EP 0850044 [0001] EP 0850044 [0001]

Claims (12)

Verfahren zur starken Anreicherung von Aminosäuren und/oder Oligopeptiden auf der Grundlage von Süssmolkepulver, das in Wasser aufgelöst wird und bei dem die vorhandenen Milchzucker- und/oder Eiweißmengen abgebaut werden, gekennzeichnet dadurch, dass mindestens eine Protease oder ein Milchsäurebakterium den Zucker zu Milchsäure umwandelt und die Eiweiße zu Oligopeptiden und Aminosäuren abbaut, wobei das notwendige saure Medium durch die Milchsäure entsteht oder erzeugt wird durch Zufügung einer anderen Säure, einer säurebildenden Substanz und oder einen anderen Säurelieferanten.Process for the strong accumulation of amino acids and / or oligopeptides based on sweet whey powder, which is dissolved in water and in which the existing Milk and / or protein quantities are degraded, characterized by that at least one protease or a lactic acid bacterium converts the sugar to lactic acid and the egg whites degrades to oligopeptides and amino acids, with the necessary Acid medium is produced or produced by the lactic acid is made by adding another acid, one acid-forming substance and or another acid supplier. Verfahren gemäß Anspruch 1, gekennzeichnet dadurch, dass zwei Proteasen oder Milchsäurebakterien zum Einsatz kommen, wobei eine zuerst den Milchzucker zu Milchsäure umwandelt, wodurch der pH-Wert gesenkt wird, die andere bevorzugt das Eiweiß abbaut.Method according to claim 1, characterized by that two proteases or lactic acid bacteria are used come, taking a first the milk sugar to lactic acid which lowers the pH, the other preferred the protein breaks down. Verfahren nach Anspruch 1 und/oder 2, gekennzeichnet dadurch, dass mindestens eine Protease als Mikroorganismus vorliegt.A method according to claim 1 and / or 2, characterized in that at least one protease is present as a microorganism. Verfahren nach Anspruch 1 und/oder 2 und/oder 3, gekennzeichnet dadurch, dass der oder die Mikroorganismen aus der Familie der bekannten und auch in der Milchwirtschaft eingesetzten Milchsäurebakterien stammen.Method according to claim 1 and / or 2 and / or 3, characterized in that the one or more microorganisms from the Family of known and also used in the dairy industry Lactic acid bacteria come. Verfahren nach Anspruch 1 bis 4, gekennzeichnet dadurch, dass die Aminosäuren in hoher Konzentration als Monoaminosäuren und Oligopeptide mit 2–9 Aminosäureneinheiten vorliegen. Method according to claims 1 to 4, characterized by that the amino acids are in high concentration as monoamino acids and oligopeptides with 2-9 amino acid units available. Verwendung der nach den Ansprüchen 1 bis 5 hergestellten Aminosäurenmischung zur Herstellung von Lebensmittel und/oder Getränken.Use of according to claims 1 to 5 prepared amino acid mixture for the preparation of Food and / or drinks. Verwendung der nach den Ansprüchen 1 bis hergestellten Aminosäurenmischung zur Herstellung von kosmetischen Produkten und/oder Körperpflegeprodukten und/oder Tierfutter und/oder Reinigungsprodukten und/oder Pharmastoffen.Use of according to claims 1 to produced Amino acid mixture for the production of cosmetic products and / or personal care products and / or animal feed and / or Cleaning products and / or pharmaceutical substances. Verwendung der nach den Ansprüchen 1 bis 5 hergestellten Lösungen als Ausgangsstoff für ein Trockenprodukt, das selbst als Ausgangsstoff für Anwendungen nach Anspruch 6 und 7 eingesetzt wird.Use of according to claims 1 to 5 prepared solutions as starting material for a dry product, itself as a feedstock for applications is used according to claim 6 and 7. Verwendung der nach Anspruch 1 bis 6 und 8 hergestellten Aminosäurenmischungen zum direkten Verkauf an Endverbraucher für deren eigene Mischungsrezepte.Use of the product according to claims 1 to 6 and 8 Amino acid mixtures for direct sale to end consumers for their own mixture recipes. Verfahren nach den Ansprüchen 1 bis 6, dadurch gekennzeichnet, dass die Protease/n enthalten in suspendierten in vitro Zellen vorliegt/vorliegen, dass diese Zellen in ein Nährmedium inokuliert und zur Bildung eines Kulturmediums kultiviert werden.Process according to claims 1 to 6, characterized characterized in that the protease / s contained in suspended in present / present in vitro cells, that these cells in a nutrient medium inoculated and cultured to form a culture medium. Verfahren nach den Ansprüchen 1–6, gekennzeichnet dadurch, dass es anaerob gehalten wird und dass die Reaktionstemperatur zwischen 20 und 40 Grad liegt, besonders bevorzugt bei 30–36 Grad C und/oder dass dabei gerührt wird.Process according to claims 1-6, characterized in that it is kept anaerobic and that the Reaction temperature between 20 and 40 degrees, more preferably at 30-36 degrees C and / or that is stirred. Verfahren nach den Ansprüchen 1–5 und 10–11, gekennzeichnet dadurch, dass der Produkt am Ende einer speziellen Filtration unterzogen wird, besonders bevorzugt einer Umkehrosmose oder einer Mikrofiltration.Process according to claims 1-5 and 10-11, characterized in that the product on End of a special filtration is subjected, particularly preferred reverse osmosis or microfiltration.
DE102007024852A 2007-05-29 2007-05-29 Strongly enriching the amino acids and/or oligopeptides based on sweet whey powder, which is dissolved in water, comprises converting sugar to lactic acid and proteins to oligopeptides and amino acids using protease or lactic acid bacterium Withdrawn DE102007024852A1 (en)

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DE102007024852A DE102007024852A1 (en) 2007-05-29 2007-05-29 Strongly enriching the amino acids and/or oligopeptides based on sweet whey powder, which is dissolved in water, comprises converting sugar to lactic acid and proteins to oligopeptides and amino acids using protease or lactic acid bacterium

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DE102007024852A DE102007024852A1 (en) 2007-05-29 2007-05-29 Strongly enriching the amino acids and/or oligopeptides based on sweet whey powder, which is dissolved in water, comprises converting sugar to lactic acid and proteins to oligopeptides and amino acids using protease or lactic acid bacterium

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2819940B1 (en) 1978-05-06 1979-06-13 Henry Dipl-Ing Zimzik Process for the production of detergent substances based on whey using lactic acid sticks
DE3924559A1 (en) 1989-07-25 1991-05-16 Nikolaus W Prof Dr Med Klehr Use of whey in cleaning and care compsn. - for feminine hygiene, using careful control of pH
EP0850044A1 (en) 1995-09-15 1998-07-01 Advance AG Process for obtaining amino acids from proteins and use of the amino-acid-containing products obtained

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2819940B1 (en) 1978-05-06 1979-06-13 Henry Dipl-Ing Zimzik Process for the production of detergent substances based on whey using lactic acid sticks
DE3924559A1 (en) 1989-07-25 1991-05-16 Nikolaus W Prof Dr Med Klehr Use of whey in cleaning and care compsn. - for feminine hygiene, using careful control of pH
EP0850044A1 (en) 1995-09-15 1998-07-01 Advance AG Process for obtaining amino acids from proteins and use of the amino-acid-containing products obtained

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