DE102011006610B3 - Aptamere, die spezifisch sind für Immunoglobulin bindende Zellwandproteine - Google Patents

Aptamere, die spezifisch sind für Immunoglobulin bindende Zellwandproteine Download PDF

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Publication number
DE102011006610B3
DE102011006610B3 DE201110006610 DE102011006610A DE102011006610B3 DE 102011006610 B3 DE102011006610 B3 DE 102011006610B3 DE 201110006610 DE201110006610 DE 201110006610 DE 102011006610 A DE102011006610 A DE 102011006610A DE 102011006610 B3 DE102011006610 B3 DE 102011006610B3
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Prior art keywords
protein
aptamer
aptamers
binding
seq
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Expired - Fee Related
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DE201110006610
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German (de)
English (en)
Inventor
Dr. Stoltenburg Regina
Beate Strehlitz
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Helmholtz Zentrum fuer Umweltforschung GmbH UFZ
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Helmholtz Zentrum fuer Umweltforschung GmbH UFZ
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Priority to DE201110006610 priority Critical patent/DE102011006610B3/de
Priority to EP12713665.3A priority patent/EP2691527A1/fr
Priority to PCT/EP2012/055655 priority patent/WO2012130951A1/fr
Application granted granted Critical
Publication of DE102011006610B3 publication Critical patent/DE102011006610B3/de
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56944—Streptococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56938—Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE201110006610 2011-03-31 2011-03-31 Aptamere, die spezifisch sind für Immunoglobulin bindende Zellwandproteine Expired - Fee Related DE102011006610B3 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE201110006610 DE102011006610B3 (de) 2011-03-31 2011-03-31 Aptamere, die spezifisch sind für Immunoglobulin bindende Zellwandproteine
EP12713665.3A EP2691527A1 (fr) 2011-03-31 2012-03-29 Aptamères ayant une spécificité pour les protéines de la paroi cellulaire qui fixent l'immunoglobuline
PCT/EP2012/055655 WO2012130951A1 (fr) 2011-03-31 2012-03-29 Aptamères ayant une spécificité pour les protéines de la paroi cellulaire qui fixent l'immunoglobuline

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE201110006610 DE102011006610B3 (de) 2011-03-31 2011-03-31 Aptamere, die spezifisch sind für Immunoglobulin bindende Zellwandproteine

Publications (1)

Publication Number Publication Date
DE102011006610B3 true DE102011006610B3 (de) 2012-06-21

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DE201110006610 Expired - Fee Related DE102011006610B3 (de) 2011-03-31 2011-03-31 Aptamere, die spezifisch sind für Immunoglobulin bindende Zellwandproteine

Country Status (3)

Country Link
EP (1) EP2691527A1 (fr)
DE (1) DE102011006610B3 (fr)
WO (1) WO2012130951A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103048369A (zh) * 2013-01-18 2013-04-17 江南大学 一种基于还原氧化石墨烯-纳米金复合材料的金黄色葡萄球菌无标记电化学适配体传感器
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
JP2023528551A (ja) * 2019-11-13 2023-07-05 クロスライフ テクノロジーズ インク． 操作されたｇｙｒｉ様ムテインアプタマー、および関連する方法
CN111004805B (zh) * 2019-12-30 2023-08-01 广西医科大学 T细胞免疫检查点pd-l1的适配体筛选、鉴定方法及抗肿瘤应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US20050250094A1 (en) 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
DE10336809B4 (de) 2003-08-07 2007-08-02 Charité - Universitätsmedizin Berlin EKG-System zur grossflächigen Messung von EKG-Signalen
US7645582B2 (en) * 2006-07-21 2010-01-12 Hitachi Chemical Co., Ltd. Aptamers that bind to listeria surface proteins
KR100930974B1 (ko) * 2007-09-27 2009-12-10 고려대학교 산학협력단 레티놀 결합 단백질(ｒｂｐ4)에 특이적으로 결합하는ｄｎａ 앱타머 및 그 제조방법
KR101086026B1 (ko) * 2008-11-27 2011-11-23 고려대학교 산학협력단 비스파틴에 특이적으로 결합하는 ｄｎａ 앱타머 및 그 제조방법
JP5804304B2 (ja) * 2008-12-09 2015-11-04 国立大学法人東京農工大学 Ｃ反応性タンパク質結合性アプタマー及びその用途
KR101024860B1 (ko) * 2009-01-15 2011-03-31 고려대학교 산학협력단 디클로페낙에 특이적으로 결합하는 ｄｎａ 앱타머 및 그 제조방법
KR101097365B1 (ko) * 2009-06-02 2011-12-23 고려대학교 산학협력단 키랄 구조의 이부프로펜에 입체선택적으로 결합하는 ｄｎａ 앱타머 및 그 제조방법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CAO, X. u.a.: Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus. Nucleic Acids Res. (2009) 37 (14) 4621-8 *
KIM, D.C. u.a.: Molecular recognition and specific interactions for biosensing applications. Sensors (2008) 8 (10) 6605-6641 *
KULBACHINSKIY, A.V.: Methods for selection of aptamers to protein targets. Biochemistry (Mosc). (2007) 72 (13) 1505-18 *

Also Published As

Publication number Publication date
WO2012130951A1 (fr) 2012-10-04
EP2691527A1 (fr) 2014-02-05

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