DE102009056944A1 - RNA-Aptamere, die den löslichen Interleukin-6-Rezeptor spezifisch binden - Google Patents

RNA-Aptamere, die den löslichen Interleukin-6-Rezeptor spezifisch binden Download PDF

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Publication number
DE102009056944A1
DE102009056944A1 DE200910056944 DE102009056944A DE102009056944A1 DE 102009056944 A1 DE102009056944 A1 DE 102009056944A1 DE 200910056944 DE200910056944 DE 200910056944 DE 102009056944 A DE102009056944 A DE 102009056944A DE 102009056944 A1 DE102009056944 A1 DE 102009056944A1
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DE
Germany
Prior art keywords
aptamer
rna
sil
rna aptamer
binding
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Withdrawn
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DE200910056944
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German (de)
English (en)
Inventor
Ulrich Prof. Dr. Hahn
Stefan Prof. Dr. Rose-John
Cindy Meyer
Tijana Zivkovic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Christian Albrechts Universitaet Kiel
Universitaet Hamburg
Original Assignee
Christian Albrechts Universitaet Kiel
Universitaet Hamburg
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Application filed by Christian Albrechts Universitaet Kiel, Universitaet Hamburg filed Critical Christian Albrechts Universitaet Kiel
Priority to DE200910056944 priority Critical patent/DE102009056944A1/de
Priority to PCT/DE2010/001412 priority patent/WO2011069486A1/fr
Publication of DE102009056944A1 publication Critical patent/DE102009056944A1/de
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE200910056944 2009-12-07 2009-12-07 RNA-Aptamere, die den löslichen Interleukin-6-Rezeptor spezifisch binden Withdrawn DE102009056944A1 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE200910056944 DE102009056944A1 (de) 2009-12-07 2009-12-07 RNA-Aptamere, die den löslichen Interleukin-6-Rezeptor spezifisch binden
PCT/DE2010/001412 WO2011069486A1 (fr) 2009-12-07 2010-12-06 Aptamères d'arn se liant spécifiquement au récepteur soluble de l'interleukine 6

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE200910056944 DE102009056944A1 (de) 2009-12-07 2009-12-07 RNA-Aptamere, die den löslichen Interleukin-6-Rezeptor spezifisch binden

Publications (1)

Publication Number Publication Date
DE102009056944A1 true DE102009056944A1 (de) 2011-06-09

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Family Applications (1)

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DE200910056944 Withdrawn DE102009056944A1 (de) 2009-12-07 2009-12-07 RNA-Aptamere, die den löslichen Interleukin-6-Rezeptor spezifisch binden

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DE (1) DE102009056944A1 (fr)
WO (1) WO2011069486A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019813A1 (fr) 1990-06-11 1991-12-26 The University Of Colorado Foundation, Inc. Ligands d'acide nucleique

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019813A1 (fr) 1990-06-11 1991-12-26 The University Of Colorado Foundation, Inc. Ligands d'acide nucleique

Non-Patent Citations (17)

* Cited by examiner, † Cited by third party
Title
Ellington und Szostak (1990), Nature 346, 818-822
Fischer, M., et al., I. A bioactive designer cytokine for human hematopoietic progenitor cell expansion. Nat Biotechnol, 1997. 15(2): p. 142-5
Gopinath (2007), Anal. Bioanal. Chem. 387, 171-182
Hirota, H. et al., (1995) Proc. Natl. Acad. Sci. U.S.A, 92, 4862-4866
Jones et al. (2001), The FASER Journal 15, 43-57
JONES, S.A., HORIUCHI. S., TOPLEY, N., [u.a.]: The soluble interleukin 6 receptor: mechanisms of production and implications in disease, 2001, In: FASEB, Vol. 15, S. 43-58 *
Jostock, T., et al., Soluble gp130 is the natural inhibitor of soluble interleukin-6 receptor transsignaling responses. Eur J Biochem, 2001. 268(1): S. 160-7
Mackiewicz, A. et al. (1992) J. Immunol., 149, 2021-2027
Osborne et al. (1997), Curr. Opin. Chem. Biol. 1, 5-9
Rose-John et al., (2006), J. J. Leukocyte Biol. 80, 227-235
Saito, M. et al. (1992 J. Immunol., 148, 4066-4071
Scheller und Rose-John, Med. Microbiol. Immunol. (2006), 195, 173-183
Scheller, J., Grötzinger, J., and Rose-John, Stefan, Zytokinsignale über lösliche und membranständige Rezeptoren. Biospektrum, 2007, 13. Jahrgang: S. 250-253
Tags, T. et al. (1989), Cell, 58, 573-581
Tuerk, C. und L. Gold, Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science, 1990. 249(4968): S. 505-10
ULRICH, H.: DNA and RNA aptamers as modulators of protein function, 2005 Vol. 1, S. 199-208, Abstract *
Version 3.2, Zuker, M., Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 2003. 31(13): p. 3406-3415

Also Published As

Publication number Publication date
WO2011069486A1 (fr) 2011-06-16

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R118 Application deemed withdrawn due to claim for domestic priority

Effective date: 20120608