DE102004034474A1 - Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren - Google Patents

Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren Download PDF

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Publication number
DE102004034474A1
DE102004034474A1 DE102004034474A DE102004034474A DE102004034474A1 DE 102004034474 A1 DE102004034474 A1 DE 102004034474A1 DE 102004034474 A DE102004034474 A DE 102004034474A DE 102004034474 A DE102004034474 A DE 102004034474A DE 102004034474 A1 DE102004034474 A1 DE 102004034474A1
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Germany
Prior art keywords
nucleic acids
column
buffer
hollow body
membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
DE102004034474A
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German (de)
English (en)
Inventor
Thorsten Singer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Priority to DE102004034474A priority Critical patent/DE102004034474A1/de
Priority to JP2007520771A priority patent/JP5230196B2/ja
Priority to PCT/EP2005/007726 priority patent/WO2006008085A1/de
Priority to US11/572,076 priority patent/US8685742B2/en
Priority to EP05761702.9A priority patent/EP1771242B1/de
Publication of DE102004034474A1 publication Critical patent/DE102004034474A1/de
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
    • B01D15/125—Pre-filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/28033—Membrane, sheet, cloth, pad, lamellar or mat
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28078—Pore diameter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28078—Pore diameter
    • B01J20/28085—Pore diameter being more than 50 nm, i.e. macropores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/86—Sorbents applied to inner surfaces of columns or capillaries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G01N2030/527—Physical parameters structural properties sorbent material in form of a membrane
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/177692—Oxides of nitrogen
    • Y10T436/178459—Only nitrogen dioxide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Nanotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE102004034474A 2004-07-15 2004-07-15 Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren Ceased DE102004034474A1 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE102004034474A DE102004034474A1 (de) 2004-07-15 2004-07-15 Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren
JP2007520771A JP5230196B2 (ja) 2004-07-15 2005-07-15 核酸のより効率的な単離のための器具および方法
PCT/EP2005/007726 WO2006008085A1 (de) 2004-07-15 2005-07-15 Vorrichtung und verfahren zur effizienteren isolierung von nukleinsäuren
US11/572,076 US8685742B2 (en) 2004-07-15 2005-07-15 Apparatus and method for the more efficient isolation of nucleic acids
EP05761702.9A EP1771242B1 (de) 2004-07-15 2005-07-15 Verfahren zur effizienten isolierung von nukleinsäuren

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102004034474A DE102004034474A1 (de) 2004-07-15 2004-07-15 Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren

Publications (1)

Publication Number Publication Date
DE102004034474A1 true DE102004034474A1 (de) 2006-02-16

Family

ID=35058358

Family Applications (1)

Application Number Title Priority Date Filing Date
DE102004034474A Ceased DE102004034474A1 (de) 2004-07-15 2004-07-15 Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren

Country Status (5)

Country Link
US (1) US8685742B2 (https=)
EP (1) EP1771242B1 (https=)
JP (1) JP5230196B2 (https=)
DE (1) DE102004034474A1 (https=)
WO (1) WO2006008085A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202015104264U1 (de) 2014-08-13 2015-10-23 Axagarius Gmbh & Co. Kg Vorrichtung zur Aufreinigung von Nukleinsäuren

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2527216A1 (en) * 2003-05-28 2004-12-09 Qiagen Gmbh Process for obtaining plasmid dna by means of an aqueous 2-phase system
DE102004034474A1 (de) 2004-07-15 2006-02-16 Qiagen Gmbh Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren
ATE468917T1 (de) 2007-10-31 2010-06-15 Hoffmann La Roche Verfahren und vorrichtung zur reinigung von nukleinsäuren
US10883100B2 (en) * 2011-03-29 2021-01-05 Phynexus, Inc Devices and methods for plasmid purification
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
RU2729113C2 (ru) 2014-11-21 2020-08-04 Оккам Байолэбс, Инк. Система и способ сбора образца нуклеиновой кислоты
WO2023056558A1 (en) * 2021-10-06 2023-04-13 Fredsense Technologies Corp. Methods and kits for isolating nucleic acids

Family Cites Families (21)

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Publication number Priority date Publication date Assignee Title
US4647380A (en) * 1982-09-22 1987-03-03 Dasgupta Purnendu K Annular dual membrane permselective device and method
JP2816739B2 (ja) * 1989-03-23 1998-10-27 旭光学工業株式会社 ウイルス及び細胞の分離器
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
JPH0436198A (ja) * 1990-05-31 1992-02-06 Shimadzu Corp 食中毒菌の検査方法
EP0461477A1 (en) 1990-05-31 1991-12-18 Shimadzu Corporation Method of bacterial examination and apparatus therefor
DE4139664A1 (de) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren
US5503816A (en) * 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
US5578459A (en) * 1993-11-24 1996-11-26 Abbott Laboratories Method and apparatus for collecting a cell sample from a liquid specimen
DE19512369A1 (de) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh Vorrichtung zur Isolierung von Nukleinsäuren
WO1998059076A1 (en) * 1997-06-25 1998-12-30 Promega Corporation Method of isolating rna
DE19734135A1 (de) * 1997-08-07 1999-02-11 Boehringer Mannheim Gmbh System zum Zurverfügungstellen biologischer Materialien
JP2000162208A (ja) * 1998-11-27 2000-06-16 Fuji Photo Film Co Ltd ガラス繊維濾紙の製造方法
DE19856064C2 (de) * 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
JP2002209580A (ja) * 2001-01-15 2002-07-30 Jsr Corp 核酸の分離方法および球状担体
JP2002345465A (ja) * 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 核酸精製ユニット及び核酸精製方法
US6818762B2 (en) * 2001-05-25 2004-11-16 Maine Molecular Quality Controls, Inc. Compositions and methods relating to nucleic acid reference standards
JP2002191351A (ja) * 2001-10-19 2002-07-09 Hitachi Ltd 核酸の精製用装置および核酸捕捉用チップ
EP1321176A1 (en) 2001-12-18 2003-06-25 Boehringer Ingelheim International GmbH Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale
US7407742B2 (en) * 2002-02-27 2008-08-05 Sanko Junyaku Co., Ltd. Plasma or serum separator, plasma or serum sampling method, plasma or serum separating method, test carrier and glass fiber
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
DE102004034474A1 (de) 2004-07-15 2006-02-16 Qiagen Gmbh Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202015104264U1 (de) 2014-08-13 2015-10-23 Axagarius Gmbh & Co. Kg Vorrichtung zur Aufreinigung von Nukleinsäuren
EP2985062A1 (de) 2014-08-13 2016-02-17 AXAGARIUS GmbH & Co. KG Vorrichtung zur aufreinigung von nukleinsäuren
DE102014216016A1 (de) 2014-08-13 2016-02-18 Axagarius Gmbh & Co. Kg Vorrichtung zur Aufreinigung von Nukleinsäuren

Also Published As

Publication number Publication date
US8685742B2 (en) 2014-04-01
EP1771242B1 (de) 2020-04-22
US20080039619A1 (en) 2008-02-14
JP5230196B2 (ja) 2013-07-10
EP1771242A1 (de) 2007-04-11
WO2006008085A1 (de) 2006-01-26
JP2008506932A (ja) 2008-03-06

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Effective date: 20110711

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