CS754487A1 - Method of saccharose hydrolysis by means of immobilized invertase - Google Patents

Method of saccharose hydrolysis by means of immobilized invertase Download PDF

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Publication number
CS754487A1
CS754487A1 CS877544A CS754487A CS754487A1 CS 754487 A1 CS754487 A1 CS 754487A1 CS 877544 A CS877544 A CS 877544A CS 754487 A CS754487 A CS 754487A CS 754487 A1 CS754487 A1 CS 754487A1
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CS
Czechoslovakia
Prior art keywords
caxapo3h
hydrolysis
pactbop
saccharose
sucrose solution
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CS877544A
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English (en)
Inventor
Joachim Rombach
Helmut Kluge
Alfred Schellenberger
Joachim Herrmann
Thomas Ernestin
Brigitte Klich
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Joachim Rombach
Helmut Kluge
Alfred Schellenberger
Joachim Herrmann
Thomas Ernestin
Brigitte Klich
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Application filed by Joachim Rombach, Helmut Kluge, Alfred Schellenberger, Joachim Herrmann, Thomas Ernestin, Brigitte Klich filed Critical Joachim Rombach
Publication of CS754487A1 publication Critical patent/CS754487A1/cs

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K3/00—Invert sugar; Separation of glucose or fructose from invert sugar

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Securing Of Glass Panes Or The Like (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)

Description

Ha3BaHHe Η300Ρ6Τθηηη
Mstou rřmpojiH3a caxapo3H npn noMomu Hwoóiura3OBaiiHoíí HHBepTasH OÓJiaCTL· ΙΓΡΗΜθΗΘΗΗΗ Η300ΡΘΤΘΗΗΗ Μ3θόρΘτθΗΗθ KacaeTca μθτοΑβ zyia πολυήθηηη cmscgíí rJiDKO3íí/$pyK-TO3H/caxapO3H H3 CaxapO3H npH HOMOIUH ΗΜΜ00ΗΛΗ30ΒΘΗΗ0β ΗΗΒθρ—Ta3H. Tairae cmgch rjnDKO3n/$pyicTO3H/caxapo3u b προ^κθ BCTpena-jqtch b ΒΗβθ TOKoro ΗΗΒβρτΗΟΓο caxapa 3M caxapHoro cupona H . . HBZLÍHQTCfl BaJKHUM IipOMeJKyTOHHHM ΠρΟβγΚΤΟΜ B ΠΗΙξθΒΟδ, φθ,ρΜΘΒΤΚ-ΗΘΟΚΟδ Jí XJÍMHHeCKOS ΙφΟΜΗΠΚΘΗΗΟΟΤΗ, OKU HCnOJIB3yK)TCa πρπ ΠΡ0Η3-ΒΟβΟΤΒΘ KOBflHTQpCKBX H3flejm2, ΗΒΠΗΤΚΟΒ, aHTHÓHOTHKOB, φθρΜΘΗ-TOB, ΓΟΡΜΟΗΟΒ Η Ιφ.
XapaKTepgoToa η3βθοτηηχ τθχηηηθοκηχ ροιπθηη# Η3Β3οτηο óoJiBiuoe μηοκθοτβο ΜθτοβΟΒ rnflpMH3a caxapo3H npH no- MOip HW4OÓHOT3OBaHHO0 HHBepTa3ÍJ, OTJIOaWXCH CJIOCOÓOM HMMO- ’ ÓIUIIí3aiBIH H HanOJIHJÍTOJIHMH* HHBepTa3a,: nanpuMep, πρπ πομοιιιη aucopÓBHH οβηβηββθτοη Mai<po-nopHCTHM H0H00ÓM6HHHK0M Ha Óa3G UOJIHCTHpGHa /AMÓGpjJHT HPA 93/ . / er-ps 2 233 334/ mra kocthhm yiuiGM / GB-PS 564 270/, ΚρΟΜΘ ΤΟΓΟ, H3BGCTHH MGTOflH M^OÓHJIHSaiW ΠρΗ ΠΟΜΟ^Η KOHBa-JIGHTHOS CBH3H. Β.ΚΒΗΘΟΤΒΘ npHMGpa MOÍtHO Ha3BaTB CBH3B Máifflóíi'Kpyiiofi /fl.MoHcan, XKomÓo jí Atamane, T., Bioteohnol. Bioeng, 658 /1984/, n.MoHcas H #,KomÓc, Biotechnol. Bioeng. 347 /1984/ H MaKponopHCTHM ΠΟθ/θΜΗΗΟΜΘΤΙ3ϋΙΟΤΗρθΗΟΜ//3θφΒΤΗΤΪΦ 58/ Al.MaHC$ejiBjj h A.ffleJuieHÓeprep, Acta Biotechnoi. 89 /1986//, . ;
BkJIIOHGHHG ΗΗΒθρΤΒ3Η B ΠΟΛΗΜθρΗΗβ MaTPW, ΗΒΠρΗΜθΡ,, i«GJiaTHHl_/A.H.KpayBe,? zesh. Nauk ser i £8, 39 /1981//. mra aJiÉWHaOajiB-HHH /O.JÍHHKO, JI.BeKUITpeM II n.JlHHKO, Food. Process Eng. Ind.1979, 81/ ΗΒΛΗΘΤΟΗ eme OJíHHM ΜΘΤΟβΟΜ ftJIH ΠΟΛΤΉΘΗΗΗ ΗΜΜΟ0ΗΛΗ3Ο-Baniíoro φθρΜβΗτβ. 2 B 3aJBHCHM0CTH OT MeTO^a HMM0ÓHJIIÍ30BaHHH H HOCHTGJie^ ÓOJIBIUHBCT-BO H3BG0THHX ΚΟΜΙϋΙΘΚΟΟΒ HOCHTGJieH HHBGpTa3H HJIH rií#pOJIH3a Ca-xapo3H hmgiot phh ηθηοοτητκοβ, Taic hto ηοποΛβ3οβθηηθ hmmoóh-λη3οβθηηοΜ BHBepTa3H b tgxhhhgckom MacuiTade nporpeccupyeTΟΗβΗΒ Μθ&amp;ΗΘΗΗΟ.
Taicne He^ocTaTKH Moryr upohbhtbch b we, ΜθχβΗΟθοκοίί cT'a-ÓH7IBH0CTH H CTaÓHJIBHOCTH ΟΤβθΛΒΗΗΧ ΤΘΧΗΟΛΟΓΗΗΘΟΚΗΧ OHGpaHHĚ . KOMHHGKOOB HOCHTGJiet HHBepTa3U, ΠΡΗΗΘΜ HOCJienHflfl B ÓOJIBUIOHMepe 3aBHCHT ot hhctoth h KoímenTpaiw cyócTpaTa, η ot tgm-nepaTypu peawm. Οτθπθηβ ejihhhhh 3HaHGHH0 pH na, cTaójuiBHOCTBonepanHĚ Taiae θββηοητ ot κοηπθητρθπηη cyócTpaTa.
KaTajiHTHHecKHíi 3φφβκτ Φθρμθητοβ Tawice 3ββηοητ ot 3HaneHHíi pE.1Rza HHBepTa3H flaeTca onTHMyM pH bgjihhhho8 ot 4,0 jio 5,5 /B.XapTMařiep,; Gornian mt. Πιώιιθβηθ npo^yKTH η τθχηολογηη imme-BLIX npojiyjKTOB 2θ» 320 /1978//, npHW npH HMMOÓHJIII3aHHH B 3a-BHCHMOCTH OT ΜΘΤΟβΗ HMM0ÓHJIH30BaHHH Η HOCHTGJIH ΠρΟΗΟΧΟΠΗΤ 0ΜΘ-ΙΙίθΗΗθ OnTHMyMa pH. JUh ycTajHOBJíeHHH onTHMyMa pE? ποθτομ^ b hsbgcthhx μθτοηθχ jyiaφθρΜθΗΤΗΟΓο rimpoJiH3a caxapo3H npeflycMOTpeHN gmkocth ληη pa3-MeuiHBaBHH, KOTopue, οληθκο, OTpHwejiBHo cKa3HBaioTca Ha Henpe-PHBHOCTB H 9K0H0MH0ÓTB ΜΘΤΟβΗ,Β CMHCJIG TpyHOGMKOOTH H CyMMHKaimTaHOBHOJKeHHĚ. .
Cxóoe ΟΤΡΗΕ^ΤΘΛΒΗΗΘ CTOpOHH HaÓJIlOWTCH ΠρΗ yCTaHOBTieHHH 3Ha-hghhž pH paoTBopoB oaxapa c ηοηοο0μθηηιικ8μη, κοτορκθ ncnojiB- ,3yraTCH b’ caxapHQ# προμηκκθηηοοτη jyin ynaneHiiH h3bgcth h otíec-i
- QQJlHBaHHH. —-----------------L Π3ΒΘ0ΤΗΗΘ ΜΘΤ0ΗΗ TaK5K0 ΓΟΒΟρΗΤ O TOM, HTO OHH AGĚCTByíQT yOUTHMyMa pE COOTBGTCTByWÍÍ HMM0ÓHJIH30BaHHQS HHBepTa3H, XOTHCpu H3BGCTHHX ΤΘΧΗΗΗΘΟΚΗΧ yCJIOBHHX 3TO 3HaHGHHG pH. OHGHB TpyH-HO yCTaHOBHTB Η Π00Τ0ΗΗΗ0 COÓJWfliaTB.
IlejIB Η300ΡΘΤΘΗΗΗ IíejIBH) Η3Ο0ρθΤθΗΗΗ HBJIHGTCH 3K0H0MH0G Η ΗΘΠρβρΗΒΗΟΘ ΠρΟΒΘβθΗΗΘrimpOOTSa CaxapO3H πρπ ΠΟΜΟΙξΗ HMM0ÓEJIH30BaHH0ÍÍ ΗΗΒβρΤΘΒΗ ΠρΗhgóojibiohx 3aTpaTax Ha annapaTH h pynHoíí Tpyn h hokjhow ho-Π0ΛΒ30Β3ΗΗΘ ΠρΗΜΘΟΘΖ flJIH nOJiyHGHHH OMGOGŘ ΓΛΚ)ΚΟ3η/ΦΡ3ΓΚΤΟ3Η/
3 caxapo3H bhcokoh KOHUGHTpauo η hhctoth., H3JI0?KGHHG OVUIHOCTH Ε3Ο0ΡΘΤΘΗΗΗ
I Β OCHOBG H30ÓpGTGHJÍH JIGJKHT 3£ζΠ£ΉΒ paGpaÓOTKH ΠρΟΟΤΟΓΟ Β ΟΤ-ηοιηθηηη annapaTypN η ηθπρθρηβηογο MeTojia ληη γηπροληθθ caxa-ρο3Η nocpejccTBQM ΗΜΜΟόΐϋΐΗβΟΒΗΗΗοδ: ηηβθρτβ3η, β pe3yjn>TaTG κοToporo mojkho nojiywTt jtokhS hhbgpthnS caxap min cupon bncokoSKOHUeHTpamiH H HHCTOTH. 3a#ana peinaeTcn tgm, hto HcnoJiB3yeTcn 50-70 $-HNt, a npemviy-ujgctbghho 60-70 %—hhS pacTBop caxapo3H,
PacTBop caxaposu co cneuH$n^ecKofí Harpy3K0H b 0,5-2,0 hπροχοηπτ ηβρθ3 oóecnBewBaíomyio KOjioHHy, KOTopaa HanojraeHa oónh-HNM ΚρθΠΚΗΜ Óa30BNM OÓGCnBGHHBaíQiHHM HOHOOÓMGHHHKOM, ΗΘΠρΗΜθρ,Bo$aTHTOM EA 60, c uejifcK) η3ΓΟΤοβλόηηη jýin rHnpojiH3a cyócTpaTaTaKOfi HHCTOTN,. HTOÓH ΒΟ-ΒρθΜΗ ΒΗββρίΚΚΗ HMM0ÓHJIH30BaHH0S HH-BepTa3H BO3fletoBHe HecaxapHCTHX bguigctb na bpgmh bnegpjkkh 'ówio hckjhohgho. HanpaaneHHe jtboghhh npn qtom mojkgt npoxo-ΠΗΤΒ KGK ΒΒθρΧ,ΤθΚ Η BHH3, C ΤΟΉΚΗ 3ΡΘΗΗΗ 3K0HDMOGCK0r0 Η0Π0ΛΒ30ΒΘΗΗΗ GMKOCTH CMOJINΠΡΗ OÓGCUBGHHBaHHH KJiepOBKH H CHpOHá, KaK H3BGOTHO, yCTanaB-JIHBaiOTCH ΠΟ B03M0JKH0CTH ÓOJIGG BHCOKHG ΟΠΘΙξΗφϋΗΘΟΚΗΘ Harpy3KH,HaXOflJBIÍHGCH, B 3aBHCHMOCTH OT OKpaCKH, B 30HG 3HaHGHKÍÍ MGJKfly2 H 6 h~-£, HpH 9T0M Ο0ΗΉΗΟ ΠρΟΗΟΧΟΛΗΤ JIHIHL HG3HaHHTGJH>H0GyMGHBIHGHHG 3HaHGHH&amp; pB Ha 0,3, ΚΟΤΟρΟβ BN3BaH0 OÓMGHHOÉ θβ-oopdiwft, πρκ KOTOpOH aHHOHH,. HM6I0IHH6CH B HGJIHHHH B HGÓOJIBHIHXKOHUGHTpaiíHSX COJIGÍÍ KHCJIHX COGOTGHE$, OÓMGHHBaKJTCH C ΗΟΗβΜΗXJIOPHHOB Λί.ΙΠΤΘΜόθρΓ H-B.BaJIBTGp, ΟόβΟΗβΗΗΒΘΙΟΠΙΗΘ OMOJIH,,. IÍ3J(.AKaflGMH-$Gpúiar, ΕθροΗ, 1970/. yHHMGHHG BH3HBaGT TOT φθΚΤ, ΗΤΟ ΠρΗ ΟόβΟΗΒΘΗΗΒΒΗΗΗ OÓHHHHXpacTBopoB caxapo3H co οπθίξΗφκΗβοκοϋ HarpysKofi: oóeciíBenHBaiomežcmojih - 0,5.-2,0 ii”1 HaójnoKaGTCfl cnan 3HanGHHíí pB ot 0,5 #02,,0 GflHHHH pH.,; HBJIHIOIHHHCn flOCTaTOHHHM flJIH HX BBOHa Β rH£pOJIH3COOTBGTCTBGHHO. OnTHMyMy HMM0ÓHJIH30BclHH0ÍÍ ΗΗΒθρΤΘ3Η, ΒΚΖΠΟΗβΗΘΓΟ BGPXHIOK) IHIOCKOCTB, B OÓJiaCTH pB OT 4,0 £0 7,0. 4
Hapj^y c Ha3BaHHot aflcopónnea oÓMeHa onpeflejieHnyio poal urpa-iot h flpyrne θφφθκτκ, Hanpmviep, TepMHHecKHfí pacnafl Hecaxapuc-thx βθαθοτβ, b pe3yflLTaTe κοτοροΓΟ n HaóAioflaeTCH ηθοοαθηηοBHCQKHÉ Cnafl 3HaWJHÉ pH. B UpOTHBOJIOAOJKHOCTL flaHHHM Η3ΒθΟΤΗΗΧ ΤΘΧΗΗΉΘΟΚΗΧ ρθΙΗΘΗΚΗ COM-H9HH9 ΒΗ3ΗΒΒΘΤ ΤΟΤ φΗΚΤ, ΗΤΟ ΓΗΑΡΟΑΗ3 caxapO3H ΠρΗ Η3ΒΘ0ΤΗΗΧΤΘΧΗΗΗΘΟΚΗΧ yCJIOBHHX ΜΟ5ΚΘΤ ÓHTL ΠρΟΒΘΑΘΗ C yfl0BA9TB0pHTeflLH0aΟΤΘΠΘΗΒΙΟ ΠρθΒρβΙξθΒΗίΙ TOULKO y OHTHMyMa pH ΗΜΜΟ0ΗΑΗ3ΟΒ3ΗΗΟ^ΗΗΒθρτβΒΗ. Οκορθθ Bcero Ha,n;o πρθΑΠΟΑΟΟΤΒ, τ,,θ. óluio Hai&amp;eHo,HTO mflP0JIH3 B OÓflaCTH, COOTBQTCTByWfí ΟΠΤΗΜΥΜΖ» ΒΚΧΙΟΗΒΗBepxHroio ero baockoctb, cooTBeTCTBytomeft ηηβθρτβ3η, npoTeKaeTόθ3 HaófliQflaeMoro 3HaHHTejiLHoro υμθηβιιιθηηη οτθπθηη npeBpameHHH.ΠρΗ 9ΤΟΜ CfleflyQT HCXOflHTL H3 Toroř ΗΤΟ CTaÓHALHOCTL ΤθΧΗΟΑΟ-ΓΗΗΘΟΚΗΧ onepaiflia COOTBGTOTByiOflea ΗΜΜΟ0ΗΑΗ3ΟΒθΐίΗθβ ΗΗΒθρΤΗ3Η.no ΒΟθδ ynoMHHyToS oóflacTH pH. cpaBHHMa co CTaóHjiLHocTLio y on-THMyMa;.
Htqóh flodHTLCfl naHóoflee βοθμομηογο πρη0αη3Κθηηη κ onTWW pHΗΜΜΟ0ΗΑΗ3ΟΒΒΗΗθίί ΗΗΒβρΤΒ3Η, paÓOTa ΠΟ COOTBeTCTByiOfleWiy H30Ópe-τθηηιο MeTOfly πρθημ^θοτβθηηο βθαθτοη co οπθηη<ΦηηθοκοΜ Harpy3-koS oóecflBeHHBaHHH MejKfly 0,5 h 1,0 h
Cpaey nocji© θτογο ο0θοιϊβθηθηηη0 pacTBop caxapo3H c ycTanoBJíeH-HHM 3ΗΗΗΘΗΗΘΜ pH, Η3ΒΘΟΤΗΗΜ OÓpa3OM npOXOflHŤ Ηθρβ3 HHBOpTHpy-ioqyio KOAOHHy, KOTopan HanoflHeHa ημμο<5ηαη3οβθηηο^ ηηβθρτββο^,HanpKMep, κομπαθκοομ HHBepTa3n BO$aTHTa y 58, πρηηθμ oópa3y-lOTCfl ΟΜΘΟΗ rflI0ÉO3IíZ5pyKTO3H/caxapO3H, flOflfl HHBepTHpOBamíOrO .caxapa κοτορκχ 3Ββηοητ οτ οπθΐίπφΗΗΘοκοκ Harpy3KH HHBepmpy-ioqea κοΑΟΗΗΗ, οτ κοηηθητρηπηη h TeMnepaTypH pacTBopa caxapo-3H, η Μο&amp;θτ óktl ycTanoBneHa coímacHo JKejiaHHio noKynaTejieS,
HanpaBJíeHne αβηκθηηη πρπ θτομ μο&amp;θτ Óktl Kax BBepx, TaK hBHH3. BoJILUIO© 3ΗΗΗΘΗΗΘ npH ΑΟΟΤΗ3ΚΘΗΗΗ ΠΡΟΟΤΟΤΗ ΠρΟΒθΑΘΗΗΗ co—OTBeTCTByiOflQIO Η300ρθΤΘΗΗΚ) MCTOfla ΗΜΘΘΤ ΤΟΤ φθΚΤ, HTO flflflycTaHOBJíeHHfl 'βΗβΗθΗΗ# pH ne TpeóyeTcn floóaBowx· θμκοοτθ# hXHMHKaTOB,, H Τ8Κ8£Θ TO, HTO B9CL npoflecc, HaHHHaa OT npH9MapacTBopa caxapo3H ao nojiyneHKfl κοηθηηογο npoflyKTa, npoTeKaeTÓecnpepHBHO. / i
I ΠΌΗΜθ-ρ ocvmecTBuemiH Η3ο6ρθτθηηη
Cjie^yioiupie npmviepH ocyiuecTBtfeHHH η300ρθτθηηη ójioe noncumoTCOOTBeTCTByWífi Κ300ρθΤθΗΗΚ) ΜΘΤΟβ.· B KanecTBe ημμο0ηο3οβηηηογο Φθρμθητβ ncnojn>3yeTCH κομπεθκοHHB©pTa3H y 58, CTaÓHJIBHOCTB ΚΟΤΟΡΟΓΟ B ΤΘΧΗΟΛΟΓΗηθΟΚΗΧ ΟΠβ-parw b 62 $-hom pacTBope caxapo3H npn 70°C, βηρθϊκθηηθη . Bpe-μθηθμ nojiypacnana, οοοτθβοθτ 131 a , a onTHMywi pH -4,5/4í,MaHC$ejiBfl n A.fflejuieHÓeprep, Acta Biotechnoi. £,97 /1986//.He3aBKCHM0 ΟΤ MeTOaa HMM0ÓHJIH3aUJIH Η ΗΟΟΗΤΘΛθΕ ΠΡΗΜΘΗΘΗΗΘ MO-ryT Ha0TH KOMimeKCH HocnTejieíí nHBepTa3H, KaTajiHTUHecioiíi 9φ-φθΚΤ H CTaÓHJIBHOCTB ΤθΧΗΟΛΟΙΌθΟΚΗΧ OnepaHHÍÍ KOTOPHX B OÓJiaCTHMeawy 4,0 h 7,0 cpaBHHBaioTCH c KaTajiHTHHecicuM θφφθκτοΜ h cTa-ÓHJIBHOCTBIO y ΟΟΟΤΒΘΤΟΤΒγίΟΙΠΘΓΟ OHTHMyMa.
Εριιμθρ I ΦΟΒΤρΟΒΒΗΗΗίί paCTBOp 66- $-HOÍÍ CaxapO3H CO 3HaH0BH©M pB - 8,03a 100 nacoB npoxofíHT nepe3 odeciíBentiBaiomyio KOJioHHy, Hanoji-HeuHyio 50 JiHTpaMH coBHoro óa30Boro oóeciíBeHUBaiomero noHoodMeH·HHKa Bc^THTa EA 60 β φορΜθ xjiopw. Padow TeivmepaTypa co- 'cTaBiineT 80°C, οπθΰΗφιπθοκβΗ Harpysica - 1,0. a”1, oKpacxa pac-TBopa B ορθβΗθΜ - 50-100 ICUMSA / Int. Coaua. Methods Su- , ΠΡΚΗΘΜ EflHHHim ICUMSA = E^gg . IO3/. gar Anal
Hocjie oóecnBewBaHHH ocTaeTCH ocTaTonnaa OKpacKa - 14-203Ha^eHH© pB pacTBopa caxapa b ορθληθμ cocTawineT 6,8 , ηθμEOCTKrae.TOH cna# 3HaHeHHt pH b 1,2 θλ. pH. lípu θτομ 3ΗβηθηηηpB ΟόθΟΙίΒθΗΘΗΗΗΜ paCTBOp CaxapO3U 3aT3M ;npOXOflHT nepes HHBep-THpyxqyra KQJiomiy, Hanojraemiyra 50 JiHTpaw KOMWieKca ιιηβθρτβ3ηy 58. Padonaa TeivmepaTypa cocTaanaeT 40°C, οπβΐϊΒφκΗθοκδυι na-rpysica - 1,0. u”1. KocTHTHyTaa οτθπθηβ otíMGHa πρϋ stom cocTaB-jirqt 89-94 %,
Ecjih cpaBHHTBr to npH coiuiacoBaHHoÉ οπθφΐφκΗβοκοδ Harpysice HHBepTHpyrameS kojiohhh, a Tarase TeMnepaTypn w KOHneHTpanjiH y
OHTHMyMa pB HMM0dHJIH30BaHH0&amp; ΗΗΒθρΤΘ3Η HOCTHTaeTCH Ο0ΜΘΗ B 89-95 %. ΟπρθκθΛθΗΗθ oóMeHa προκοχο^ΗΤ no odfceMHóaHaHHTireeoKOMy μθτο- 'ť'*i 6 'l
Ay M.X.MH-QííHÓHa / Official Methods of Anály sis , 494;
Aspociation of Official AgriculturáL Oheraists , BaJUMHTTOH, Jt.K./1965// jyiH οπρθαθαθηηα peAynHpoBaHHHX. caxapoB. 3ηβαθηηη pH.onpesejuuiHCB ποτθηιαιομθτρηηθοκημ nyyeM. Πόημθό 2 . ΟόθΟΤ-ΐΒΘΉΙίΒαΗΪΙΘ Η. ΗΗΒΘρΤΗρΟΒΒΗΙίθ ΠρΟΒΟβΗΤΟΒ, ΚΒΚ Π0ΚΒ3ΗΗ0 ΒπρΗΜβρθ I.
Hcxoahhm nponyKTOM ηβααθτοη 67 $-ηηη pacTBop caxapo3H co _3Ha-ηθηηθμ pB - 6,9, ΟπθίξΗφκΉθοκθΒ: Harpy3Ka oóeciíBeHHBaiiHfl η ηη-BepTHpoBaaHH cocTaanaeT 0,5 h coxpaHHBmaaca. ocTaTOwaOKpacKa hocaq ο0θοαβθήηββηηη cocTawincT 14-20 %, «HocraraeTcacnan 3HaH0HHĚ pH B 1,7 ΘΑΗΗΗΐχ.ρΗ, ΤΜ^ΉΤΟ ΓΗΑΡ0ΑΗ3 ΠρΟΤΘΚΒθΤπρκ pH - 5,2. Πρκ 9tom flocTHraeTCH oómqh b 95-98 %, cootbotot-ByroinHH oóMeny y onTHMywia pB ημμο0ιιαη3οββηηο0: nHBepTa3H npH•OflHHaKOBOíi οπΘίξκφΗΉθοκοίί Harpy3Ke HHBepTHpyiomeS kojiohhh, aTaioKe TewinepaTypH h KOHueHTpaumí pacTBopa oaxapo3H, ΠΌΗΜΘΌ 3 ’ ' ;··^ OóeciíBeHHBaHHe h nHBepTHpoBaHHe npoBOAOTca, kbk noKa3ano bnpHMepe I. f ,
KoHneHTpanHH pacTBopa caxapo3u οοοτββααθτ 67 %„ cnenH$snecKaHHarpysKa ο0θοαβθήηββηκη η ηηβθρτηροββηηα - 1,5 coxpaHHB-iuaHCB ocTaTOHHaa OKpacKa nocjie odecnBeHHBamia 14-20 %, BHaneraie pH pacTBopa uocjie odecnBeAHBaHHH b cpe^neM cocTaB-jiaeT 6,1 , αθμ AOCTuraeTca cnaA 3ηβαθηιι&amp; pH Β Ι,Ι θα. pH. Πρβ-BpameBHe wieamy 78 h 82 % cooTBeTCTByeo? TOMy, κοτοροθ AOCTura-eTca πρκ oAHHaKOBoň ΌΠθΐίΗφιΐΗβοκο0 Harpy3Ke HHBepuHpyiomea ko-λοηηη, a Tajíce TewinepaType h KOHiíeHTpamni pacTBopa caxapo3Hy OUTHMyMa pH HMM0ÓBAH30BaHH0É ΗΗΒθρΤΒ3Η, Πρημθό 4 v ΟόθΟΕξΒΘΉΗΒβΐυΐθ Η ΗΗΒθρΤΙίρΟΒΒΗΗΘ ΠρΟΒΟβΗΤΟΗ, Κ3Κ II0Ka3aH0 Β npHMepe I. Ηοχοληβμ nponyKTOM cnyxníT 66 $-hn0 pacTBop caxapo3H, 'kotophčínpoxoflHT nepee odecuBeWBaiomyio h HHBepTHpyiomyio kojiohhh co cne-ΐΒϊφΗΗθοκοδ Harpy3Koa - 2,0 η”1, πρηηβΜ^ποωΐθ odecnBentíBaHuncoxpaHHGTCH 16-20 $ oCTaTOHHoS OKpacKH, a 3HaneHne pH. pacTBo*pa caxapo3H cocTaBJLHeT b cpenneM 4,2 , ητο cooTBeTCTByeT cna#y3HaHeinďi pH b 0,8 θα, npeBpaineime Me?my 71 h 76 % m b upene^ax, flocTHraeMHX yOIITHMyMa pH HMMOdHJIH3OBaHHOt ΗΗΒβρΤΒ3Η npH OJÍHHaKOBoS cneují-φηηβοκοΐί narpy3Ke rniBeprapyiomefí kojiohhh, a Tarase TewinepaTypeη KOHneHTpanHH pacTBopa caxapo3H. Πρημθρη 5-12 $iunjTpoBaHHHií pacTBop caxapo3H npoxoAHT Hepee odecnBeHHBafflinyioKOJiOHHy, HanojiHeHHyio 250 mu οοβηογο óa3OBoro oóecnBenHBaiome-ro HOHOoÓMeHHHKa BO$aTHTa EA 60 b φορΜθ xjiopw npn TeMnepa-Type 80°C. .IIocjie oóeciíBewBaHHH ocTaTonHan oKpacKa cocTaBna-6T 11-38 %> 3aranoHeHne φΗΛΒτροΒθΗΗηίι η οόθΟΓξΒθΗθΗΗΗίί pacTBop CaxapO3H_—.. προχο^κτ nepe3 HHBepTupyiomyra KOJioHHy, KOTopas conepoT. 250 mji KOMEneicca nnBepTa3H Bo$araTa 7 58 npn TewinepaType 40°C, βοοτητ-nynie pe3yjiLTaTU, Kacaiomnecn cna^a 3Hanenníi pH nočně oóecnBe-HHBaHHH H OČMOHa, ΟΒΘ,ΣξθΗΗ B TaÓJIHUy I. IÍ3 3ΤΟΓΟ BK£HO, HTOnpn προ mix οαηηοκοβηχ ycjioBnnx ηθτ sHanuTejiLHHX pasjrantó wiejs-ny npeBpameHHHMH, onpenejieHHHWM b oójiacTH pB. - 4,0 n 7,0 , ny onTHMywa pB KOMiuieKca HHBepTa3H Bo$aTHTa 7 58. Β3ητηθ npoó npoBOwocB nocne HaoTyroiennn cTannoHapnoro cocto-hhhh, npunewi onpe^ejteHne npeBpan|eHna προΒΟβίαοοΒ ΦθρμθητηημΜβΤΟβΟΜ rJII0KO3a-OKCH£a3a-nepOKCH£a3a-£HaHH3OTH. SnaHeHHH pBonpeflejianncB ποτθηφϊονϊθτριϊηθοκημ nyrewu
.Γ> ΦοΡΜν,πα Η3Ο0Ρ6ΤΘΗΜΗ I. Μθτοη nmpo^H3a caxapo3ti πρη ποΜοιηπ ™oóoH30BaHHoK hh-Βθρτα3Η, Γβθ pacTBop caxapo3n cHa^ana προχοτριτ ηορθ3 oóec-uBe^HBaiomyio KOJiouHy, HanojiHenHyio chjibhhm óa3 oblím oóocnBe-HHBaiOqHM ΗΟΗΟΟ0ΜΘΗΗΗΚΟΜ, 3aTOM lIOftBOpraeTCH ΓΗβρ0Ο33Γ,, O T JI H H "a 10 HJ Jí &amp; O Η Τ θ Μ ,. ΉΤΟ 50-70 %-HHÍÍ pac-tbop oaxapo3H προχοβΗΤ Ηθρθ3 otíeciíBe^íBaromyio Kojioímy cocnei]#$oecKoSi narpý3KOíí ot 0,5 λο 2,0 , 3aT0M oóec·*· ΐΧΒΟΉΘΗΗΗδ pacTBop CaxapO3U npn 3HaH0HHH pH OT 4,0 RO 7,0HORBepraeTca riw;pojiH3yi 2; Motojí no nyHKTy I, o τ ji η n a io m % # c a τ e m , htonpeHMynjecTBeííHo HcnojiB3yeTCH 60-70 $-hh0 pacTBop caxapo3u. 3. Μθτο# no nyHKTawi I h 2,; oTJiHHaiomníicH τ e m ,ητο οπθίξκφΗΗβοίΦΗ Harpy3Ka oóeonBenHBaiomeíi kojiohhh npen-MymecTBOHHo cocTaBJíneT 0,5-1,0 h i
Πί *-&amp;
• -W , Λν“.W· ·. Ρ63Ι0ΜΘ
OnncHBaQTCH μθτοα ri'mpojiH3a caxapoau πρπ ποΜοιηκ hmmoóoh3o-BanHoÉ HHBepTa3H, πρΗ κοτοροΜ oóecuBewBaeTCfl η ΗΗΒθρτΗργθτ—ch 50-70 %-hhm pacTBop caxapo3u, íIocpeacTBOM HacTOBmero μθτο-#a MOJKHO nOJiyTOB CMOCH rjn0KO3u/$p3TKTO3u/caxapO3U, JIBJIHIOmHe-ca ΒευκΗΗΜκ προΜθκγτοΉΗΗΜΗ npoayKTaMK nraqeBot,, $apwianeBTm©e-KOfl H XMHeCKOÉ ΠρΟΜίώϋΙΘΗΗΟΟΤΗ. HO CpaBH6HIW C ApyTHMH H3-ΒΘΟΤΗΗΜΗ ΜΘΤΟβθΜΗ flaHHHÍi MOTOfl γπρΟΉθΗ, COTJiaCHO Κ3Ο0ΡΘΤΘΗΗΙ0οπθΐϊΗφΗ^θοκΗΗ Harpy3Ka oóeciíBewBaMnefi kojiohhu, HanojiHOHHoS .COBHHM OÓeCIÍBeaHBaiQmiIM HOHOOÓMGHHHKOM - paCTBOpOM OCHOBaUHH,ycTanoBJíeHa TaKHM oópa3OM, wo, 3HaaeHHa pH cyócTpaTa hoojigoóecnBeanBaHHH jiestaT b oÓJiacTH ot 4,0 50 7,0 , ητο cootbgtot-ByeT onTHMyMy nMMoÓEJiH3OBaHHoM HHBepTa3n, BKJiioaaa θθ βθρχηιοιοehockoctb'.· Tami oópa3OM rapaHTHpyeTca HenpepuBHaa paóoTa, Ha-WHaa. c npEGMa pacTBopa caxapo3u ao noayaeHHa κοηθήηογο npo-ayKTa. s* βθοκριπιτορΗ: ΕηΟΤΘΧΒΟΛΟΓΗΗ,; TGXHHHGCKaa Φθρμθητολογηη, nMMoóajiH3OBaHHUG ^opMeHTH^MGdE7iH30BaHHaa "HHBspTasa^^KOBajieHTHoe οοθ^ηηθηκθ ,ttoojih3 caxapowrijTS^ne^w^cMóaáT ' óóéoWηηβθηηθ ,' čně-"ΐίκφΗΕθοκθΗ HarpysKa, 3HaasHne pH, HEBeprapoBaHiíe, Bpewia bu-aepjKKH. ΙΓηπΊΌπιψίΜ iciwnercTnýe-t iist.’p:řír:;-.uvrcjz, «no nopeuij/i’ KCMeuuofiy opnnuíajiy. r. Ijťpjniir, '/9/ΜΛ Jí)//,·. I.N . , * f hitj . ,i, * ' r t . x v '. - « d . f i;;),'· r» /0,- (λΐίΛ1 ;s , , • ; J l yi<· rpynjiw; 'n/y ITepuwwwK: • 1 . i : í;r lííjfl

Claims (3)

  1. PŘEDMĚT VYNÁLEZU
    1. Způsob hydrolýzy sacharosy pomocí immobilizované in-vertázy, kde roztok sacharosy nejprve prochází přesodbarvovací kolonu, naplněnou silným bázovým odbar-vovacím ionovým výměníkem, potom se podrobuje hydro-lýze, se vyznačuje tím, že 50-70 %~tni roztok sacharosy prochází přes odbarvovací kolonuse specifickým zatížením od 0,5 do 2,0 n-\ potom od-barvený roztok sacharosy při hodnotě pH od 4,0 do 7,0se podrobuje hydrolýze.
  2. 2. Způsob podle bodu 1, se vyznačuje tím,že především se používá 60 - 70 %tní roztok sacharosy.
  3. 3. Způsob podle bodu la2, se vyznačujetím, že specifické zatížení odbarvovací kolony jepředevším 0,5 - 1,0 n“\
CS877544A 1986-11-20 1987-10-20 Method of saccharose hydrolysis by means of immobilized invertase CS754487A1 (en)

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US7435564B2 (en) * 2003-09-29 2008-10-14 Instituto Technologico Y De Estudios Superiores De Monterrey Production of invert syrup from sugarcane juice using immobilized invertase

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GB564270A (en) * 1943-03-15 1944-09-20 Tate & Lyle Ltd Improvements in and relating to the inversion of sugar liquers
BE792041A (fr) * 1971-11-30 1973-03-16 Adeola Ag Dispositif d'inversion pour la commande du piston d'un groupe moteur pneumatique
FR2233334A1 (en) * 1973-06-15 1975-01-10 Anvar Ion exchange resin contg. adsorbed invertase - bonded firmly to the resin for saccharose hydrolysis
US4085587A (en) * 1975-11-03 1978-04-25 Leslie H. Garlinghouse Fail safe liquid power device
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PL268884A2 (en) 1988-08-18
FI875077L (fi) 1988-05-21
AT392979B (de) 1991-07-25
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FI875077A7 (fi) 1988-05-21
CU21934A3 (cs) 1996-10-05
FR2607151A1 (fr) 1988-05-27
HUT46027A (en) 1988-09-28

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