CS272496B1 - Method of substrate pasteurization made from cotton waste and mixture of cotton waste with other lignocellulosic materials by means of selective fermentation - Google Patents

Method of substrate pasteurization made from cotton waste and mixture of cotton waste with other lignocellulosic materials by means of selective fermentation Download PDF

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CS272496B1
CS272496B1 CS884893A CS489388A CS272496B1 CS 272496 B1 CS272496 B1 CS 272496B1 CS 884893 A CS884893 A CS 884893A CS 489388 A CS489388 A CS 489388A CS 272496 B1 CS272496 B1 CS 272496B1
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waste
cotton
substrate
cotton waste
pasteurization
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CS884893A
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Czech (cs)
Slovak (sk)
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CS489388A1 (en
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Anna Ing Lazarova
Anastazia Rndr Csc Ginterova
Miroslav Ing Zilincik
Olga Ing Kvopkova
Ludovit Ing Mikula
Marian Ing Grestiak
Jan Ing Frtus
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Anna Ing Lazarova
Anastazia Rndr Csc Ginterova
Miroslav Ing Zilincik
Olga Ing Kvopkova
Ludovit Ing Mikula
Marian Ing Grestiak
Jan Ing Frtus
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Priority to CS884893A priority Critical patent/CS272496B1/en
Publication of CS489388A1 publication Critical patent/CS489388A1/en
Publication of CS272496B1 publication Critical patent/CS272496B1/en

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Abstract

Riešenie sa týká aposobu pasterizácia subatrátov, ktorých hlavnou zložkou ja bavlněný odpad, pre ziskanie biomasy alebo metabolitov húb, selektivnou fermentáciou. Vychádza zo schopnosti samozáhrevu odpadovej bavlny, ku ktorému dochádza po úpravě teploty a vlhkosti^na optimálně podmlsnky pre kličsnie spor mikroorganizmov(iktoré sa v odpadovej bavlně nachádzajú.Trojstupňgvou selektivnou fermentáciou za anaerobně aerobnych podmianok sa substrát biologicky očistí a po ochládáni vzduchom zbaveným zárodkov naočkuje kultúrou húb. Riešenie znižuje energetické náklady na pasterizáciu subatrátov, ktorých hlavnou zložkou je odpadová bavlnThe solution relates to a pasteurization process subatrates whose main component is i cotton waste, to obtain biomass or. \ t metabolites of fungi, by selective fermentation. It is based on self-heating ability waste cotton that occurs after adjusting the temperature and humidity to optimum conditions for worm spores of microorganisms (though are in the waste cotton selective fermentation under anaerobic aerobic conditions the substrate is biologically cleaned and post-treated inoculated with germ-free air mushroom culture. The solution reduces energy costs pasteurization of the subatrates, the main ones component is waste cotton

Description

Vynález sa týká sposobu pasterizácie substrátu z bavlněného odpadu určeného pre získanie biomasy alebo metabolitov húb, pričom využívá schopnost samozáhrevu odpadovej bavlny po úpravě vlhkosti a teploty.The present invention relates to a method of pasteurizing a substrate from cotton waste intended to obtain biomass or fungal metabolites, using the self-heating ability of the waste cotton after adjusting the humidity and temperature.

Lignocelulózově materiály sa vo všeobecnosti stávajú vhodným substrátom pře kultiváciu húb po úpravě vlhkosti na 70% a tepslnom ošetření, ktorého cielom je zničenie zárodkov prirodzenej mikroflóry východiskového materiálu. Prirodzená mikroflora je tvořená zárodkami mikroorganizmov a hmyzu. V súčasnosti sa substráty pre pestovanie húb, fermentované alebo náfarmentované, ošetrujú parou vyrábanou vo vyvíjačoch na rozny zdroj energie. Pře pestovanie hlivy je takéto ošetrenie substrátu jednou z najdrahšich technologických operácií.Lignocellulosic materials generally become a suitable substrate for the cultivation of fungi after adjusting the humidity to 70% and heat treatment, the aim of which is to destroy the germs of the natural microflora of the starting material. The natural microflora is formed by the germs of microorganisms and insects. At present, substrates for mushroom growing, fermented or fermented, are treated with steam produced in various energy source generators. For growing mushrooms, such treatment of the substrate is one of the most expensive technological operations.

Novým substrátom pře pestovanie húb je bavlněný odpad, ktorý vzniká ako druhotná surovina v textilnom priemysle. 3e známy sposob pestovania jedlých húb na textilných a iných lignocelulózových materiálech, kedy sa substrát připravuje z bavlněného alebo iného lignocelulózového materiálu pomocou pasterizácie alebo sterilizácie z vonkajšieho zdroja energie.A new substrate for mushroom growing is cotton waste, which is generated as a secondary raw material in the textile industry. 3e is a known method of growing edible mushrooms on textile and other lignocellulosic materials, wherein the substrate is prepared from cotton or other lignocellulosic material by pasteurization or sterilization from an external energy source.

Doležitou vlastnosťou bavlněného odpadu je jeho schopnost samozáhrevu, ku ktorému dochádza po úpravo vlhkosti a teploty, ktoré sú optimálně pre klíčenie spor mikroorganizmov v odpade přítomných. Z tejto vlastnosti vychádza nový sp'osob přípravy substrátov. Odpadová bavlna sa može fermentovať s cielom ziskania vhodného substrátu pře biotechnologie alebo samotná alebo v kombinácii s inými lignocelulózovými odpadmi textilného priemyslu (odpady po spracovaní lanu, konop, juty) drevárskeho priemyslu (piliny), alebo polnohospodársksj prvovýroby. Známy sposob anaeróbno aeróbnej fermentácie substrátu pre pestovanie húb nezahrnuje fermentáciu bavlněného odpadu, ktorý je zvlášť vhodný pře .samozáhrev bez pridania akýchkolvek přísad podporujúcich samozáhrsv a nezahrnuje možnosti takto připraveného substrátu pre pestovanie iných druhov húb na získanie vzácných metabolitov pre Salšie biotechnologie.An important property of cotton waste is its ability to self-heat, which occurs after adjusting the humidity and temperature, which are optimal for the germination of spores of microorganisms present in the waste. A new method of preparing substrates is based on this property. Waste cotton can be fermented in order to obtain a suitable substrate for biotechnology or alone or in combination with other lignocellulosic wastes of the textile industry (wastes after processing of rope, hemp, jute) of the wood industry (sawdust), or agricultural primary production. The known method of anaerobic aerobic fermentation of a mushroom growing substrate does not involve the fermentation of cotton waste, which is particularly suitable for self-heating without the addition of any self-heating additives, and does not include the possibility of such a prepared substrate for growing other fungi to obtain rare metabolites for safer biotechnology.

Podstatou riešenia je příprava biologicky vhodného substrátu, ktorého hlavnou složkou je odpadová bavlna. Bavlněný odpad z textilnej výroby alebo zmes bavlněného odpadu s inými lignocelulózovými odpadmi sa v tepelne izolovanom fermentore zvlhčí teplou vodou na vlhkost 50 až 60% a teplotu 40 °C. Vytvoria sa tým optimálně podmienky pře klíčenie zárodkov mikroorganizmov, hlavně baktérii v surovině. Spotrebovávajú sa živiny a kyslík, vylučuje sa oxid uhličitý, voda a teplo. Teplota materiálu stúpne nad 60 °C. Vyklíčené spory húb pri tejto teplote hynú. Materiál sa nechá anaerábne fermentovať 12 hodin, potom sa premieša, provzdušni a ochladí. Teplota sa znova nechá vyštípit na 60 °C. Fermentuje sa óalšich 12 hodin, po čom sa substrát ochladí vzduchom zbaveným zárokov.The essence of the solution is the preparation of a biologically suitable substrate, the main component of which is waste cotton. Cotton waste from textile production or a mixture of cotton waste with other lignocellulosic waste is moistened in a thermally insulated fermenter with warm water to a humidity of 50 to 60% and a temperature of 40 ° C. This will create optimal conditions for the germination of germs of microorganisms, especially bacteria in the raw material. Nutrients and oxygen are consumed, carbon dioxide, water and heat are excreted. The temperature of the material rises above 60 ° C. Sprouted fungal spores die at this temperature. The material is allowed to ferment anaerabically for 12 hours, then mixed, aerated and cooled. The temperature is again cleaved to 60 ° C. It is fermented for a further 12 hours, after which the substrate is cooled with free air.

Riešenie je možné použit všade tam, kde sa bude substrát z bavlněného odpadu použivať pre kultiváciu húb s cielom ziskania biomasy alebo metabolitov.The solution can be used wherever a substrate from cotton waste will be used for the cultivation of fungi in order to obtain biomass or metabolites.

Sposob dokumentujú ale neobmedzujú následovně příklady:The method is documented but not limited by the following examples:

Přiklad 1Example 1

Bavlněný odpad sa rovnoměrně zvlhčí rovnakým hmotnostným množstvem vody tak, aby výsledná vlhkosť materiálu bola 50 až 60% a teplota 40 °C. Materiál sa nschá formontovať v tvare s najmenším povrchom. Od vonkajšieho prostredia je materiál izolovaný. Po vystúpení teploty nad 60 °C sa materiál fermentuje 12 hodin, potom sa premieša, prevzdušni a ochladí na 40 až 45 °C. Znova sa nschá fermentovať a po vystúpení teploty nad 60 °C sa fermentácia ponechá 5 hodin. Potom sa materiál znovu premieša, provzdušni a ochladí na 40 až 50 °C a znovu sa nechá anaerobně fermentovať pri teplote nad 60 °C 12 hodin. Po ochladsni na teplotu pod 30 °C čistým vzduchom sa substrát očkuje 1,5 až 2% sadiva hlivy.The cotton waste is uniformly moistened with the same amount by weight of water so that the resulting moisture content of the material is 50 to 60% and the temperature is 40 ° C. The material can be assembled in the shape with the smallest surface. The material is insulated from the outside environment. After the temperature rises above 60 ° C, the material is fermented for 12 hours, then mixed, aerated and cooled to 40-45 ° C. It can be fermented again and the fermentation is left for 5 hours after the temperature rises above 60 ° C. The material is then mixed again, aerated and cooled to 40-50 ° C and re-fermented anaerobically at a temperature above 60 ° C for 12 hours. After cooling to below 30 ° C with clean air, the substrate is inoculated with 1.5 to 2% oyster seedlings.

CS 272 496 BlCS 272 496 Bl

Přiklad 2Example 2

Bavlněný odpad sa rovnoměrně zvlhčí rovnakým hmotnostným množstvem teplej vody, tak aby výsledná vlhkost bola 50 až 60% a výsledná teplota 40 °C. Přidá sa 2,5% sádry a po zmiešaní sa materiál nechá fermentovať pri teplote nad 60 °C 12 hodin. Potom sa ochladi vzduchom zbaveným zárodkov, premieša a znovu nechá fermentovat pri teplote nad 60 C 5 hodin. Opakuje sa chladenie, premiešanis a znova sa fermentuje pri teplote nad 60 °C 12 hodin. Po ochladeni vzduchom zbaveným zárodkov sa substrát očkuje produkčným kmenom huby produkujúcej celulózy.The cotton waste is uniformly moistened with the same amount of hot water by weight so that the resulting humidity is 50 to 60% and the resulting temperature is 40 ° C. 2.5% gypsum is added and, after mixing, the material is allowed to ferment at a temperature above 60 ° C for 12 hours. It is then cooled in germ-free air, mixed and fermented again at a temperature above 60 DEG C. for 5 hours. Cool, mix and stir again at above 60 ° C for 12 hours. After cooling with germ-free air, the substrate is inoculated with a production strain of a cellulose-producing fungus.

Claims (2)

Sposob pasterizácio substrátu z bavlněného odpadu textilnej výroby alebo zmesi bavlněného odpadu s inými lignocelulózovými materiálmi selektivnou fermentáciou pře ziskanie biomasy alebo metabolitov háb vyznačujúci sa tým, že materiál sa zvlhči na 50 až 60% vlhkost teplou vodou tak, aby výsledná teplota zvlhčeného materiálu bola 40 až 45 °C a znovuvystúpeni teploty nad 60 °C 5 hodin a po opatovnom premiešani, prevzdušnsni a ochladeni na 40 až 45 °C a vystúpeni teploty nad 60 °C Salšich 12 hodin, na čo sa zmes ochladi vzduchom zbaveným zárodkov pod 30 °C a očkuje sa produkčným kmeňom húb.Process for pasteurization of a substrate from cotton waste of textile production or a mixture of cotton waste with other lignocellulosic materials by selective fermentation to obtain biomass or metabolite of habs characterized in that the material is moistened to 50 to 60% moisture with warm water so that the resulting temperature of the moistened material is 40 to 45 ° C and a rise in temperature above 60 ° C for 5 hours and after stirring again, aeration and cooling to 40 to 45 ° C and a rise in temperature above 60 ° C for a further 12 hours, after which the mixture is cooled with germ-free air below 30 ° C and it is inoculated with a production strain of fungi.
CS884893A 1988-07-06 1988-07-06 Method of substrate pasteurization made from cotton waste and mixture of cotton waste with other lignocellulosic materials by means of selective fermentation CS272496B1 (en)

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