CS269050B1 - Lymphocyte Mice VU VIH7 - Google Patents

Abstract

The solution concerns a mouse lymphocyte hybridoma producing an IgG 2a subclass antibody against the nucleoprotein of influenza A virus. The hybridoma is stored in the hybridoma collection of the Institute of Virology of the Slovak Academy of Sciences in Bratislava under the designation VU VIH7.

Description

EN 269 050 B1 1

The invention and the invention relates to a mouse lymphocyte hybridoma, i.e., a hybrid single-cell organism, driven by cell fusion of a mouse Sp2 / 0a myeloma line, and a mouse spleen cell producing an anti-influenza A antibody,

Antibodies to influenza virus proteins have so far been prepared so that the viral or its isolated proteins are repeatedly injected as an antigen by experimental animals, most often rabbits, mice, or ferrets. The serum immunized in this way, removed after a certain period of antigen challenge, serves as a source of antibodies used primarily for the quantitative or qualitative analysis of the influenza virus - in research and practice. F

This process, termed conventional immunization, has several disadvantages. A heterogeneous mixture of antibodies whose spectrum is in each individual organism is non-repeatable is found in the sera of immunized animals. Typically, the antibody creates antibodies to the antigen and antibody to the antigenic impurity impurities needed to remove them from the sera. Consequently, batches of conventional sera can be standardized and used in a wide range of quality. For the production of each batch, pure immunization antigen and 3 other antigens were prepared to saturate the non-purification ballast antibodies.

The above drawbacks of the aforementioned and hitherto used method, when hybrid is available, produce a monoclonal antibody against influenza A virus nucleoprotein, deposited in the hybrid collection of the Virological Institute of the SAS in Brač, Mill Valley 1, under the name VUVIH7,

Said hybridoma was obtained from a known literature (Kohler, G., Milstein, C .: Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature, 256, (1975), 4951 Gerhard.W ,: Fusion of cells in suspensionand outgrowth Hybrids: A New Oimension in Blological Analysis, R, H. Kennet, T.3, McKearn, KB Bechtol, eds., Nex York, Plenum Press (1980), 370.) by cloning the hybrid cell array by fusing the mouse myeloma line Sp2 / 0 and the spleen cells derived from the BALB / c mouse immunized with influenza virus A / Dunadln / 4/73 (H3N2), the advantage of which is that it produces a homogeneous antibody, a so-called monoclonal antibody that is capable of specifically reacting with a nucleoprotein-influenza virus of type A. The VU VIH7 hybrid can be cultured in vitro in animal cell media, or in vivo in the peritoneal cavity of the BALB / c mouse, conserved Preserved in liquid nitrogen, production of antibody by more immunization with antigen may be initiated. Example

For the purpose of propagating hybridoma cells in vivo, 5 x 10 6 cells were injected into the peritoneal cavity of the mouse, For better attachment of the applied cells, the bda mouse

Claims (1)

SUBJECT OF THE INVENTION Mouse lymphocyte hybrid VU VIH7, producing monoclonal IgG subclass 2a against the nucleoprotein of influenza A virus. antibody