RU93019968A - MUS MUSCULUS HYBRID CULTIVATED ANIMAL CELLS STRAIN - A PRODUCER OF MONOCLONAL ANTIBODIES TO THE C-END PART OF PROTEIN P24 OF THE HUMAN IMMUNODEZHDITITITE VIRUS OF TYPE - Google Patents

MUS MUSCULUS HYBRID CULTIVATED ANIMAL CELLS STRAIN - A PRODUCER OF MONOCLONAL ANTIBODIES TO THE C-END PART OF PROTEIN P24 OF THE HUMAN IMMUNODEZHDITITITE VIRUS OF TYPE

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Publication number
RU93019968A
RU93019968A RU93019968/13A RU93019968A RU93019968A RU 93019968 A RU93019968 A RU 93019968A RU 93019968/13 A RU93019968/13 A RU 93019968/13A RU 93019968 A RU93019968 A RU 93019968A RU 93019968 A RU93019968 A RU 93019968A
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RU
Russia
Prior art keywords
type
protein
cells
monoclonal antibodies
human
Prior art date
Application number
RU93019968/13A
Other languages
Russian (ru)
Other versions
RU2070926C1 (en
Inventor
В.Б. Локтев
Е.Е. Коновалов
Н.В. Федюк
А.Г. Покровский
С.А. Куляндин
Original Assignee
В.Б. Локтев
Е.Е. Коновалов
Н.В. Федюк
А.Г. Покровский
С.А. Куляндин
Filing date
Publication date
Application filed by В.Б. Локтев, Е.Е. Коновалов, Н.В. Федюк, А.Г. Покровский, С.А. Куляндин filed Critical В.Б. Локтев
Priority to RU93019968A priority Critical patent/RU2070926C1/en
Priority claimed from RU93019968A external-priority patent/RU2070926C1/en
Publication of RU93019968A publication Critical patent/RU93019968A/en
Application granted granted Critical
Publication of RU2070926C1 publication Critical patent/RU2070926C1/en

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Claims (1)

Штамм гибридных перевиваемых клеток NS5E4 был получен слиянием клеток мышиной миеломы P3 NS1/1-Ag4.1 с клетками сингенных мышей BALB/c, иммунизированных лизатом клеток МТ-4, инфицированных вирусом иммунодефицита человека 1 типа. Гибридома NS5E4 секретирует моноклональные антитела (МА) IgG2a субкласса, специфически взаимодействующие с С-концевой частью молекулы белка р24 вируса иммунодефицита человека 1 типа, способные связывать указанный белок из раствора и используемые в тест-системе для определения концентрации белка р24 в биологических жидкостях. МА NS5E4 являются типоспецифическими и не реагируют с антигенами вируса иммунодефицита человека 2 типа. Штамм гибридных клеток растет в виде стационарной суспензии и вызывает асцитную опухоль у мышей BALB/c. Штамм депонирован во Всесоюзной коллекции перевиваемых клеток позвоночных ВСКК(П) под N 484Д.The strain of hybrid transplantable NS5E4 cells was obtained by fusing mouse P3 NS1 / 1-Ag4.1 myeloma cells with cells from syngeneic BALB / c mice immunized with the lysate of MT-4 cells infected with human immunodeficiency virus type 1. Hybridoma NS5E4 secretes subclass monoclonal antibodies (MA) of IgG2a, specifically interacting with the C-terminal part of the human immunodeficiency virus p24 protein type 1, capable of binding this protein from solution and used in the test system to determine the concentration of p24 protein in biological fluids. MA NS5E4 are type-specific and do not react with type 2 human immunodeficiency virus antigens. The hybrid cell strain grows as a stationary suspension and causes ascites tumor in BALB / c mice. The strain deposited in the All-Union collection of transplanted vertebrate cells VSKK (P) under N 484D.
RU93019968A 1993-04-19 1993-04-19 Strain of hybrid cultured cells mus musculus l used for preparing monoclonal antibodies to the c-terminal part of protein p24 of human immunodeficiency virus type 1 RU2070926C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU93019968A RU2070926C1 (en) 1993-04-19 1993-04-19 Strain of hybrid cultured cells mus musculus l used for preparing monoclonal antibodies to the c-terminal part of protein p24 of human immunodeficiency virus type 1

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU93019968A RU2070926C1 (en) 1993-04-19 1993-04-19 Strain of hybrid cultured cells mus musculus l used for preparing monoclonal antibodies to the c-terminal part of protein p24 of human immunodeficiency virus type 1

Publications (2)

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RU93019968A true RU93019968A (en) 1996-07-10
RU2070926C1 RU2070926C1 (en) 1996-12-27

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RU93019968A RU2070926C1 (en) 1993-04-19 1993-04-19 Strain of hybrid cultured cells mus musculus l used for preparing monoclonal antibodies to the c-terminal part of protein p24 of human immunodeficiency virus type 1

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2094611C (en) * 1991-08-22 2000-07-25 Tsuneya Ohno Hiv immunotherapeutics
RU2134724C1 (en) * 1992-08-24 1999-08-20 Ниссин Сокухин Кабусики Кайся Humanized antibody and method of preparation thereof, polynucleotyl (versions), and hybridon cell line (versions)
RU2283497C1 (en) * 2005-04-07 2006-09-10 Общество с ограниченной ответственностью Научно-производственное объединение "Диагностические системы" (ООО НПО "Диагностические системы") IMMUNOENZYMATIC TEST SYSTEM FOR IDENTIFYING THE SPECTRUM OF ANTIBODIES TO HIV 1 AND 2 AND DETECTING ANTIGEN HIV 1 (p24) NAMED "DS-IEA-ANTI-HIV 1 AND 2, HIV 1 GROUP O-SPECTRUM+AG p24 HIV 1"

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