CO6741187A2 - Métodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas - Google Patents

Métodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas

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Publication number
CO6741187A2
CO6741187A2 CO13152090A CO13152090A CO6741187A2 CO 6741187 A2 CO6741187 A2 CO 6741187A2 CO 13152090 A CO13152090 A CO 13152090A CO 13152090 A CO13152090 A CO 13152090A CO 6741187 A2 CO6741187 A2 CO 6741187A2
Authority
CO
Colombia
Prior art keywords
compositions
methods
arnip
arn
plants
Prior art date
Application number
CO13152090A
Other languages
English (en)
Inventor
Xiang Huang
Thomas Mcneill
Michael Schweiner
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of CO6741187A2 publication Critical patent/CO6741187A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La presente invención proporciona una molécula de ARN bicatenario que comprende una hebra antisentido y una hebra sentido, donde la secuencia de nucleótidos de la hebra antisentido es complementaria respecto a una porción de la secuencia de nucleótidos del gen Hg-rps-23 de un nematodo que induce quistes en la soya, moléculas de ácido nucleico que codifican las moléculas de ARN y composiciones que comprenden las moléculas de ácido nucleico y moléculas de ARN de esta invención, así como también sus métodos de empleo para mejorar la resistencia de una planta o célula vegetal frente a la infestación e infección por parte de nematodos.
CO13152090A 2010-12-09 2013-06-26 Métodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas CO6741187A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US42127510P 2010-12-09 2010-12-09

Publications (1)

Publication Number Publication Date
CO6741187A2 true CO6741187A2 (es) 2013-08-30

Family

ID=45418816

Family Applications (1)

Application Number Title Priority Date Filing Date
CO13152090A CO6741187A2 (es) 2010-12-09 2013-06-26 Métodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas

Country Status (9)

Country Link
US (1) US20140007296A1 (es)
CN (1) CN103261423A (es)
AR (1) AR083029A1 (es)
BR (1) BR112013014082A2 (es)
CA (1) CA2818997A1 (es)
CO (1) CO6741187A2 (es)
IN (1) IN2013MN01235A (es)
RU (1) RU2013131164A (es)
WO (1) WO2012078949A2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR085359A1 (es) 2011-02-14 2013-09-25 Syngenta Participations Ag Arn pequeños de interferencia con secuencias de semilla de objetivo especifico
AR093909A1 (es) 2012-12-12 2015-06-24 Bayer Cropscience Ag Uso de ingredientes activos para controlar nematodos en cultivos resistentes a nematodos
US10889825B2 (en) 2015-02-21 2021-01-12 The Trustees Of The University Of Pennsylvania Compositions and methods thereof for down-regulation of genes following oral delivery of an RNAi molecule bioencapsulated within plant cells
US10947558B2 (en) * 2015-07-22 2021-03-16 Rutgers, The State University Of New Jersey Compositions and methods for inducing resistance to soybean cyst nematode via RNAi
WO2017123787A1 (en) * 2016-01-13 2017-07-20 University Of Massachusetts Antihelminthic medications for pathogenic nematodes
WO2019171272A1 (en) * 2018-03-05 2019-09-12 Stellenbosch University Method for controlling pest infestations
CN113614225A (zh) * 2019-12-17 2021-11-05 耐克基因有限责任公司 细小病毒载体及其制备方法和用途

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Also Published As

Publication number Publication date
AR083029A1 (es) 2013-01-23
IN2013MN01235A (es) 2015-06-12
CN103261423A (zh) 2013-08-21
US20140007296A1 (en) 2014-01-02
BR112013014082A2 (pt) 2016-11-29
WO2012078949A3 (en) 2012-08-16
CA2818997A1 (en) 2012-06-14
WO2012078949A2 (en) 2012-06-14
RU2013131164A (ru) 2015-01-20

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