CO6551678A2 - Contructo para la producción de capsides picornavirales vacias - Google Patents
Contructo para la producción de capsides picornavirales vaciasInfo
- Publication number
- CO6551678A2 CO6551678A2 CO12080730A CO12080730A CO6551678A2 CO 6551678 A2 CO6551678 A2 CO 6551678A2 CO 12080730 A CO12080730 A CO 12080730A CO 12080730 A CO12080730 A CO 12080730A CO 6551678 A2 CO6551678 A2 CO 6551678A2
- Authority
- CO
- Colombia
- Prior art keywords
- host cell
- construct
- capsid
- protease
- precursor protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4747—Apoptosis related proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/04—Inactivation or attenuation; Producing viral sub-units
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32111—Aphthovirus, e.g. footandmouth disease virus
- C12N2770/32122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32111—Aphthovirus, e.g. footandmouth disease virus
- C12N2770/32123—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/136—Screening for pharmacological compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4703—Regulators; Modulating activity
- G01N2333/4704—Inhibitors; Supressors
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Hospice & Palliative Care (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención proporciona un constructo que, cuando se expresa en una célula huésped, es capaz de producir cápsides vacías de virus, el constructo comprende: (i) una secuencia de nucleótidos que codifica una proteína precursora de la cápside; (ii) una secuencia de nucleótidos que codifica una proteasa capaz de escindir la proteína precursora de la cápside; y (iii) un elemento de control que controla la expresión de la proteasa de tal manera que, cuando el constructo está presente en la célula huésped, el elemento de control hace que la proteasa que se exprese a un nivel suficiente para escindir la proteína precursora de la cápside, pero no suficiente para inducir una toxicidad significativa en la célula huésped. La invención también proporciona un vector y una célula huésped que comprende dicho constructo y su uso para generar cápsides vacías de virus.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0918375.7A GB0918375D0 (en) | 2009-10-20 | 2009-10-20 | Construct |
Publications (1)
Publication Number | Publication Date |
---|---|
CO6551678A2 true CO6551678A2 (es) | 2012-10-31 |
Family
ID=41462637
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CO12080730A CO6551678A2 (es) | 2009-10-20 | 2012-05-16 | Contructo para la producción de capsides picornavirales vacias |
Country Status (15)
Country | Link |
---|---|
US (1) | US9243230B2 (es) |
EP (1) | EP2491118B1 (es) |
JP (1) | JP2013507948A (es) |
KR (1) | KR101818934B1 (es) |
CN (1) | CN102834507B (es) |
AU (1) | AU2010309648A1 (es) |
BR (1) | BR112012009475B1 (es) |
CO (1) | CO6551678A2 (es) |
ES (1) | ES2652443T3 (es) |
GB (1) | GB0918375D0 (es) |
HU (1) | HUE035889T2 (es) |
IN (1) | IN2012DN03093A (es) |
RU (1) | RU2565537C2 (es) |
WO (1) | WO2011048353A2 (es) |
ZA (1) | ZA201202882B (es) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB201111183D0 (en) | 2011-06-30 | 2011-08-17 | Animal Health Inst | Peptide |
KR101900460B1 (ko) | 2011-11-03 | 2018-11-08 | 센티넥스트 테라퓨틱스 에쓰디엔 비에이취디 | 인간 엔테로바이러스에 대한 백신 |
US10202423B2 (en) | 2012-09-05 | 2019-02-12 | Medicago Inc. of Quebec, Canada | Picornavirus-like particle production in plants |
US10294277B2 (en) | 2013-03-26 | 2019-05-21 | The Pirbright Institute | Stabilised FMDV capsids |
WO2015167710A1 (en) * | 2014-04-29 | 2015-11-05 | National Health Research Institutes | Adenoviral vector-based vaccine against enterovirus infection |
US11103570B2 (en) * | 2015-06-15 | 2021-08-31 | Bavarian Nordic A/S | Recombinant modified vaccinia virus Ankara (MVA) foot and mouth disease virus (FMDV) vaccine |
US9975926B2 (en) | 2015-12-08 | 2018-05-22 | The United States of America, as Represented by the Secretary of Homeland Security | Methods of making and using vaccines utilizing minicircle DNA expression vectors for production of foot-and-mouth-disease virus proteins and virus-like particles |
US10385319B2 (en) | 2016-09-08 | 2019-08-20 | The Governement of the United States of America, as represented by the Secretary of Homeland Security | Modified foot-and-mouth disease virus 3C proteases, compositions and methods thereof |
US10435695B2 (en) | 2016-09-08 | 2019-10-08 | The Government of the United States of America, as represented by the Secretary of Homeland Security | Fusion protein comprising Gaussia luciferase, translation interrupter sequence, and interferon amino acid sequences |
US10308927B2 (en) * | 2017-01-17 | 2019-06-04 | The United States of America, as Represented by the Secretary of Homeland Security | Processing of a modified foot-and-mouth disease virus P1 polypeptide by an alternative protease |
WO2019092027A1 (en) * | 2017-11-09 | 2019-05-16 | Boehringer Ingelheim Vetmedica Gmbh | Sapelovirus immunogenic compositions and uses thereof |
GB201812474D0 (en) | 2018-07-31 | 2018-09-12 | Autolus Ltd | Nucleic acid construct |
WO2020067027A1 (ja) * | 2018-09-28 | 2020-04-02 | 一般財団法人阪大微生物病研究会 | Vlp発現cho細胞株の構築 |
EP4232083A1 (en) | 2020-10-22 | 2023-08-30 | Intervet International B.V. | Baculovirus expression vector |
CN116348133A (zh) | 2020-10-22 | 2023-06-27 | 英特维特国际股份有限公司 | 杆状病毒表达载体 |
CN117897170A (zh) | 2021-08-20 | 2024-04-16 | 英特维特国际股份有限公司 | 具有稳定化突变的fmdv病毒样颗粒 |
IL310903A (en) | 2021-08-20 | 2024-04-01 | Intervet Int Bv | A method for producing a virus-like particle for foot-and-mouth disease virus |
WO2023021167A1 (en) | 2021-08-20 | 2023-02-23 | Intervet International B.V. | Method of producing a foot and mouth disease virus virus-like particle |
CN117881424A (zh) | 2021-08-20 | 2024-04-12 | 珀布赖特研究所 | 具有双稳定突变的fmdv病毒样颗粒 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19712899C1 (de) | 1997-03-27 | 1998-12-10 | Christian Dr Probst | Verfahren zur Herstellung nichtinfektiöser rekombinanter Picornavirus-Partikel |
RU2143921C1 (ru) * | 1999-04-21 | 2000-01-10 | Всероссийский научно-исследовательский институт защиты животных | Вакцина против ящура типа а и способ ее изготовления |
FR2810888B1 (fr) | 2000-06-29 | 2004-07-30 | Merial Sas | Vaccin contre la fievre aphteuse |
ES2307345B1 (es) * | 2004-01-21 | 2009-11-13 | Consejo Sup. Investig. Cientificas | Capsidas vacias quimericas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones. |
EP1632247A1 (en) | 2004-09-03 | 2006-03-08 | Consejo Superior De Investigaciones Cientificas | Nucleic acid sequences encoding FMDV proteins capable of associating into a virus-like particle |
NZ542111A (en) * | 2005-08-30 | 2008-04-30 | Otago Innovation Ltd | Novel assay |
-
2009
- 2009-10-20 GB GBGB0918375.7A patent/GB0918375D0/en not_active Ceased
-
2010
- 2010-09-24 KR KR1020127011421A patent/KR101818934B1/ko active IP Right Grant
- 2010-09-24 JP JP2012534759A patent/JP2013507948A/ja active Pending
- 2010-09-24 AU AU2010309648A patent/AU2010309648A1/en not_active Abandoned
- 2010-09-24 EP EP10777059.6A patent/EP2491118B1/en active Active
- 2010-09-24 IN IN3093DEN2012 patent/IN2012DN03093A/en unknown
- 2010-09-24 WO PCT/GB2010/001807 patent/WO2011048353A2/en active Application Filing
- 2010-09-24 RU RU2012120700/10A patent/RU2565537C2/ru active
- 2010-09-24 HU HUE10777059A patent/HUE035889T2/en unknown
- 2010-09-24 BR BR112012009475A patent/BR112012009475B1/pt active IP Right Grant
- 2010-09-24 US US13/502,014 patent/US9243230B2/en active Active
- 2010-09-24 ES ES10777059.6T patent/ES2652443T3/es active Active
- 2010-09-24 CN CN201080058064.7A patent/CN102834507B/zh active Active
-
2012
- 2012-04-19 ZA ZA2012/02882A patent/ZA201202882B/en unknown
- 2012-05-16 CO CO12080730A patent/CO6551678A2/es not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
ZA201202882B (en) | 2013-06-26 |
WO2011048353A2 (en) | 2011-04-28 |
EP2491118A2 (en) | 2012-08-29 |
JP2013507948A (ja) | 2013-03-07 |
RU2012120700A (ru) | 2013-12-10 |
KR101818934B1 (ko) | 2018-01-17 |
EP2491118B1 (en) | 2017-11-08 |
CN102834507B (zh) | 2016-09-07 |
US20120258133A1 (en) | 2012-10-11 |
KR20120069756A (ko) | 2012-06-28 |
RU2565537C2 (ru) | 2015-10-20 |
AU2010309648A1 (en) | 2012-05-03 |
CN102834507A (zh) | 2012-12-19 |
GB0918375D0 (en) | 2009-12-02 |
WO2011048353A3 (en) | 2011-09-29 |
BR112012009475A2 (pt) | 2015-09-15 |
ES2652443T3 (es) | 2018-02-02 |
IN2012DN03093A (es) | 2015-09-18 |
HUE035889T2 (en) | 2018-05-28 |
US9243230B2 (en) | 2016-01-26 |
BR112012009475B1 (pt) | 2020-06-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CO6551678A2 (es) | Contructo para la producción de capsides picornavirales vacias | |
BR112015018493A2 (pt) | Linhagens celulares para produção de vírus e métodos de uso | |
MY151062A (en) | Pseudoinfectious flavivirus and uses thereof | |
NZ597401A (en) | Chimeric influenza virus-like particles comprising hemagglutinin | |
BRPI0507700A (pt) | renderização de uma interface de usuário | |
AR083162A1 (es) | Uso de ingredientes activos para controlar nematodos en cultivos resistentes a nematodos | |
MX342388B (es) | Variantes y composiciones que comprenden variantes con alta estabilidad en presencia de un agente quelante. | |
CL2011002082A1 (es) | Composicion farmaceutica que comprende un inhibidor de nucleosido polimerasa y un inhibidor de proteasa macrociclica; proceso para preparar dicha composicion farmaceutica; metodo para tratar o mejorar una infeccion con virus de la hepatitis c, fibrosis hepatica y alteracion de la funcion hepatica. | |
GT200900097A (es) | Inhibidores de la proteasa ns3 del hcv. | |
WO2012072096A8 (en) | Method for cellular rna expression | |
CL2008003596A1 (es) | Fitasa enzimaticamente susceptible, polinucleotido que la codifica, casete de expresion, vector y celula huesped que comprenden dicho acido nucleico; metodo de preparacion de la fitasa, y composiciones que la comprenden. | |
NZ609564A (en) | Inhibitors of hepatitis c virus | |
AU2007240448A8 (en) | Methods and compositions for expressing negative-sense viral RNA in canine cells | |
PH12014502763A1 (en) | Method for the degradation of keratin and use of the keratin hydrolysate produced | |
PA8813901A1 (es) | Paramixovirus oncolíticos atenuados que codifican citoquinas de aves | |
AR070326A1 (es) | Vectores de closterovirus y metodos relacionados | |
CL2017002661A1 (es) | Método de producción de gonadotrofina | |
BR112012024224A2 (pt) | mutações da faixa de hospedeiro de flavivírus e seu uso. | |
AR114287A2 (es) | Constructo de un gen quimérico aislado; polipéptido codificado por dicho constructo; y método para conferir a una planta tolerancia a sequía, alta salinidad y congelamiento, excluida la planta obtenida por dicho método | |
CL2008003821A1 (es) | Metodo para producir el virus de la estomatitis vesicular (vsv) atenuado en celulas, que comprende: a) proporcionar una celula que comprende un gen g vsv optimizado e inducible, b) inducir la celula a expresar dicho gen, c) infectar la celula con un vsv atenuado, d) hacer crecer dichas celulas, y e) recuperar el vsv atenuado | |
PE20211303A1 (es) | Polipeptidos del factor vii de accion corta | |
MX2013012925A (es) | Metodos y composiciones para silenciar familias de genes mediante el uso de microarn artificiales. | |
CL2008003820A1 (es) | Metodo para producir el virus de estomatitis vesicular atenuado (vsv) en un cultivo celular que comprende introducir en un vector el gen vsv g optimizado; composicion inmunogenica que comprende el vsv atenuado; kit para producir el vsv. | |
MX2013013063A (es) | Plantas que tienen rasgos mejorados relacionados con el rendimiento y metodo para elaborar las mismas. | |
AR052088A1 (es) | Un promotor inducible de desoxihipusina sintetasa de maiz |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FC | Application refused |