IN2012DN03093A - - Google Patents

Download PDF

Info

Publication number
IN2012DN03093A
IN2012DN03093A IN3093DEN2012A IN2012DN03093A IN 2012DN03093 A IN2012DN03093 A IN 2012DN03093A IN 3093DEN2012 A IN3093DEN2012 A IN 3093DEN2012A IN 2012DN03093 A IN2012DN03093 A IN 2012DN03093A
Authority
IN
India
Prior art keywords
construct
host cell
protease
precursor protein
capsid precursor
Prior art date
Application number
Inventor
Charleston Bryan
Jones Ian
Original Assignee
Pirbright Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pirbright Inst filed Critical Pirbright Inst
Publication of IN2012DN03093A publication Critical patent/IN2012DN03093A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4747—Apoptosis related proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/04—Inactivation or attenuation; Producing viral sub-units
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32111—Aphthovirus, e.g. footandmouth disease virus
    • C12N2770/32122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32111—Aphthovirus, e.g. footandmouth disease virus
    • C12N2770/32123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4703—Regulators; Modulating activity
    • G01N2333/4704—Inhibitors; Supressors

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Hospice & Palliative Care (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention provides a construct which, when expressed in a host cell, is capable of producing empty virus capsids, the construct comprising: (i) a nucleotide sequence encoding a capsid precursor protein; (ii) a nucleotide sequence encoding a protease capable of cleaving the capsid precursor protein; and (iii) a control element which controls the expression of the protease such that, when the construct is present in the host cell, the control element causes the protease to be expressed at a level sufficient to cleave the capsid precursor protein, but not sufficient to induce significant toxicity in the host cell. The invention also provides a vector and a host cell comprising such a construct and their use to generate empty virus capsids.
IN3093DEN2012 2009-10-20 2010-09-24 IN2012DN03093A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0918375.7A GB0918375D0 (en) 2009-10-20 2009-10-20 Construct
PCT/GB2010/001807 WO2011048353A2 (en) 2009-10-20 2010-09-24 Construct

Publications (1)

Publication Number Publication Date
IN2012DN03093A true IN2012DN03093A (en) 2015-09-18

Family

ID=41462637

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3093DEN2012 IN2012DN03093A (en) 2009-10-20 2010-09-24

Country Status (16)

Country Link
US (1) US9243230B2 (en)
EP (1) EP2491118B1 (en)
JP (1) JP2013507948A (en)
KR (1) KR101818934B1 (en)
CN (1) CN102834507B (en)
AU (1) AU2010309648A1 (en)
BR (1) BR112012009475B1 (en)
CO (1) CO6551678A2 (en)
ES (1) ES2652443T3 (en)
GB (1) GB0918375D0 (en)
HU (1) HUE035889T2 (en)
IN (1) IN2012DN03093A (en)
PH (1) PH12012500662A1 (en)
RU (1) RU2565537C2 (en)
WO (1) WO2011048353A2 (en)
ZA (1) ZA201202882B (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201111183D0 (en) 2011-06-30 2011-08-17 Animal Health Inst Peptide
US9987350B2 (en) 2011-11-03 2018-06-05 Sentinext Therapeutics Sdn Bhd Antigens and vaccines directed against human enteroviruses
ES2911774T3 (en) 2012-09-05 2022-05-20 Medicago Inc Production of enterovirus 71-like particles in plants
AU2014243149B2 (en) 2013-03-26 2017-11-23 The Pirbright Institute Stabilised FMDV capsids
KR20170068410A (en) * 2014-04-29 2017-06-19 내셔날 헬스 리서치 인스티튜트 Adenoviral vector-based vaccine against enterovirus infection
WO2016202828A1 (en) * 2015-06-15 2016-12-22 Bavarian Nordic A/S Recombinant modified vaccinia virus ankara (mva) foot and mouth disease virus (fmdv) vaccine
US9975926B2 (en) 2015-12-08 2018-05-22 The United States of America, as Represented by the Secretary of Homeland Security Methods of making and using vaccines utilizing minicircle DNA expression vectors for production of foot-and-mouth-disease virus proteins and virus-like particles
US10385319B2 (en) 2016-09-08 2019-08-20 The Governement of the United States of America, as represented by the Secretary of Homeland Security Modified foot-and-mouth disease virus 3C proteases, compositions and methods thereof
US10435695B2 (en) 2016-09-08 2019-10-08 The Government of the United States of America, as represented by the Secretary of Homeland Security Fusion protein comprising Gaussia luciferase, translation interrupter sequence, and interferon amino acid sequences
US10308927B2 (en) * 2017-01-17 2019-06-04 The United States of America, as Represented by the Secretary of Homeland Security Processing of a modified foot-and-mouth disease virus P1 polypeptide by an alternative protease
WO2019092027A1 (en) * 2017-11-09 2019-05-16 Boehringer Ingelheim Vetmedica Gmbh Sapelovirus immunogenic compositions and uses thereof
GB201812474D0 (en) * 2018-07-31 2018-09-12 Autolus Ltd Nucleic acid construct
JPWO2020067027A1 (en) * 2018-09-28 2021-08-30 一般財団法人阪大微生物病研究会 Construction of VLP-expressing CHO cell lines
AU2021363609A1 (en) 2020-10-22 2023-05-11 Intervet International B.V. Baculovirus expression vector
US20230390380A1 (en) 2020-10-22 2023-12-07 Intervet Inc. Baculovirus expression vector
WO2023020737A1 (en) 2021-08-20 2023-02-23 Intervet International B.V. Fmdv virus-like particle with stabilizing mutation
WO2023020738A1 (en) 2021-08-20 2023-02-23 The Pirbright Institute Fmdv virus-like particle with double stabilizing mutation
IL310905A (en) 2021-08-20 2024-04-01 Intervet Int Bv Method of producing a foot and mouth disease virus virus-like particle
KR20240046569A (en) 2021-08-20 2024-04-09 인터벳 인터내셔널 비.브이. Method for producing foot-and-mouth disease virus virus-like particles

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19712899C1 (en) 1997-03-27 1998-12-10 Christian Dr Probst Process for the production of non-infectious recombinant picornavirus particles
RU2143921C1 (en) * 1999-04-21 2000-01-10 Всероссийский научно-исследовательский институт защиты животных Vaccine against foot and mouth of type a and method of its preparing
FR2810888B1 (en) 2000-06-29 2004-07-30 Merial Sas VACCINE AGAINST FOOT AND MOUTH DISEASE
ES2307345B1 (en) * 2004-01-21 2009-11-13 Consejo Sup. Investig. Cientificas CHEMICAL EMPTY CAPSIDES OF THE VIRUS CAUSING THE INFECTIOUS BURSITIS DISEASE (IBDV), ITS PROCEDURE FOR OBTAINING AND APPLICATIONS.
EP1632247A1 (en) 2004-09-03 2006-03-08 Consejo Superior De Investigaciones Cientificas Nucleic acid sequences encoding FMDV proteins capable of associating into a virus-like particle
NZ542111A (en) 2005-08-30 2008-04-30 Otago Innovation Ltd Novel assay

Also Published As

Publication number Publication date
RU2012120700A (en) 2013-12-10
KR20120069756A (en) 2012-06-28
CN102834507B (en) 2016-09-07
US20120258133A1 (en) 2012-10-11
WO2011048353A2 (en) 2011-04-28
AU2010309648A1 (en) 2012-05-03
BR112012009475A2 (en) 2015-09-15
ZA201202882B (en) 2013-06-26
ES2652443T3 (en) 2018-02-02
HUE035889T2 (en) 2018-05-28
WO2011048353A3 (en) 2011-09-29
CN102834507A (en) 2012-12-19
KR101818934B1 (en) 2018-01-17
CO6551678A2 (en) 2012-10-31
US9243230B2 (en) 2016-01-26
EP2491118B1 (en) 2017-11-08
BR112012009475B1 (en) 2020-06-09
GB0918375D0 (en) 2009-12-02
JP2013507948A (en) 2013-03-07
RU2565537C2 (en) 2015-10-20
EP2491118A2 (en) 2012-08-29
PH12012500662A1 (en) 2012-10-22

Similar Documents

Publication Publication Date Title
IN2012DN03093A (en)
MX339581B (en) Subtilase variants.
EA201100225A1 (en) NEW REGULATORY ELEMENTS
IN2014CN00688A (en)
MX352023B (en) Alpha-amylase variants and polynucleotides encoding same.
MX2013003681A (en) Engineered nucleic acids and methods of use thereof.
MY151062A (en) Pseudoinfectious flavivirus and uses thereof
MX336112B (en) Vaccines and immunotherapeutics using codon optimized il-15 and methods for using the same.
MX337984B (en) Alpha amylase variants and polynucleotides encoding same.
NZ597401A (en) Chimeric influenza virus-like particles comprising hemagglutinin
MX2015014720A (en) Psicose epimerase mutant and method for preparing psicose by using same.
MX354516B (en) Vectors encoding rod-derived cone viability factor.
MX355356B (en) Alpha-amylase variants and polynucleotides encoding same.
MX354704B (en) Alpha-amylase variants and polynucleotides encoding same.
MX2012006096A (en) Polypeptides having glucoamylase activity and polynucleotides encoding same.
EP3805348A3 (en) Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
MX2009010492A (en) Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells.
MX369726B (en) Polypeptides having protease activity.
IN2014DN07017A (en)
CO6430475A2 (en) POLIPEPTIDES THAT HAVE CELLULASE ACTIVITY
MX349517B (en) Polypeptides having protease activity and polynucleotides encoding same.
MX2014015667A (en) Use of polypeptides having protease activity in animal feed and detergents.
EA201200186A1 (en) VECTOR, CONTAINING MANNOSE PROMOTER, AND MANNOSE PROMOTER
MX350391B (en) Polypeptides having protease activity and polynucleotides encoding same.
WO2010133668A3 (en) Eukaryotic host cell comprising an expression enhancer