CO2018011808A1 - Método in vitro para identificar genotípicamente ambos alelos de al menos un locus de un gen hla en muestras de adn genómico de un sujeto - Google Patents

Método in vitro para identificar genotípicamente ambos alelos de al menos un locus de un gen hla en muestras de adn genómico de un sujeto

Info

Publication number
CO2018011808A1
CO2018011808A1 CONC2018/0011808A CO2018011808A CO2018011808A1 CO 2018011808 A1 CO2018011808 A1 CO 2018011808A1 CO 2018011808 A CO2018011808 A CO 2018011808A CO 2018011808 A1 CO2018011808 A1 CO 2018011808A1
Authority
CO
Colombia
Prior art keywords
alleles
genotypically
locus
identify
subject
Prior art date
Application number
CONC2018/0011808A
Other languages
English (en)
Inventor
Ramos Alberto Gómez
Ferrero Ricardo Rosa
Hervás Diana María Valero
Pardo Juan Carlos Triviño
Palomares Lucía Bernad
Díez Miguel Cabo
Micó María Carmen Collado
Gomis Celia Buades
Belert Manuel Gorriz
Pedrosa María Victoria Fernández
Original Assignee
Sist Genomicos S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sist Genomicos S L filed Critical Sist Genomicos S L
Publication of CO2018011808A1 publication Critical patent/CO2018011808A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

El método de la invención permite realizar un test genético a pacientes con el fin de conocer los haplotipos (combinación de alelos de un mismo loci de un cromosoma que son transmitidos juntos) de sus antígenos leucocitarios humanos (HLA) con una precisión, exactitud y fiabilidad mejorados respecto a los métodos conocidos en el estado de la técnica. La invención establece, mediante análisis bioinformático, la determinación precisa de estos haplotipos y permite saber qué individuos son compatibles para trasplantes; diagnóstico/pronóstico de enfermedades autoinmunes; tests de paternidad; reacciones adversas a fármacos en medicina personalizada; vacunación, etc.... 
CONC2018/0011808A 2018-09-18 2018-10-31 Método in vitro para identificar genotípicamente ambos alelos de al menos un locus de un gen hla en muestras de adn genómico de un sujeto CO2018011808A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18382671.8A EP3626835A1 (en) 2018-09-18 2018-09-18 Method for genotypically identifying both alleles of at least one locus of a subject's hla gene

Publications (1)

Publication Number Publication Date
CO2018011808A1 true CO2018011808A1 (es) 2020-05-05

Family

ID=64308684

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2018/0011808A CO2018011808A1 (es) 2018-09-18 2018-10-31 Método in vitro para identificar genotípicamente ambos alelos de al menos un locus de un gen hla en muestras de adn genómico de un sujeto

Country Status (2)

Country Link
EP (1) EP3626835A1 (es)
CO (1) CO2018011808A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023161633A1 (en) * 2022-02-23 2023-08-31 Nhs Blood And Transplant Method and reagents for hla typing
CN114854737B (zh) * 2022-03-11 2023-07-14 郑州大学 基于三代测序平台的i类hla基因扩增引物、试剂盒及分型方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8050870B2 (en) * 2007-01-12 2011-11-01 Microsoft Corporation Identifying associations using graphical models
JP5833922B2 (ja) * 2008-08-12 2015-12-16 ジンファンデル ファーマシューティカルズ インコーポレイテッド 疾患危険因子を同定する方法
WO2012000445A1 (zh) 2010-06-30 2012-01-05 深圳华大基因科技有限公司 新的的pcr测序方法及其在hla基因分型中的应用
WO2013011734A1 (ja) 2011-07-21 2013-01-24 ジェノダイブファーマ株式会社 Ｈｌａ遺伝子のｄｎａタイピング方法及びキット
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
JP2016010318A (ja) 2012-10-26 2016-01-21 ジェノダイブファーマ株式会社 Ｈｌａ遺伝子のｄｎａタイピング方法及びキット
WO2014116729A2 (en) 2013-01-22 2014-07-31 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of hla loci with ultra-deep shotgun sequencing
US10176294B2 (en) 2013-03-15 2019-01-08 The Broad Institute, Inc. Accurate typing of HLA through exome sequencing
JP6308724B2 (ja) 2013-05-09 2018-04-11 ジェノダイブファーマ株式会社 Ｈｌａ遺伝子のマルチプレックスｄｎａタイピング方法及びキット
CN106103738B (zh) 2013-11-27 2020-08-28 吉诺戴夫制药株式会社 利用超大规模平行测序仪进行简便的hla基因的dna分型方法和试剂盒
US20150379195A1 (en) 2014-06-25 2015-12-31 The Board Of Trustees Of The Leland Stanford Junior University Software haplotying of hla loci
WO2016054135A1 (en) 2014-10-01 2016-04-07 Georgetown University Next-generation sequencing for phased hla class i antigen recognition domain exons
JP2019530476A (ja) * 2016-09-26 2019-10-24 シロナ ゲノミクス, インコーポレイテッドSirona Genomics, Inc. ヒト白血球抗原遺伝子型決定方法およびサンプル集団におけるｈｌａハプロタイプの多様性の決定
JP6798697B2 (ja) * 2017-02-13 2020-12-09 国立大学法人京都大学 Ｈｌａ遺伝子のｐｃｒプライマーセット及びそれを用いたシークエンス法

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Publication number Publication date
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