CN86104864A - Extraction method for compound amino acid - Google Patents
Extraction method for compound amino acid Download PDFInfo
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- CN86104864A CN86104864A CN198686104864A CN86104864A CN86104864A CN 86104864 A CN86104864 A CN 86104864A CN 198686104864 A CN198686104864 A CN 198686104864A CN 86104864 A CN86104864 A CN 86104864A CN 86104864 A CN86104864 A CN 86104864A
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- amino acid
- silkworm chrysalis
- hydrolysis
- yield
- raw material
- Prior art date
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Abstract
The extraction of aminoacids complex is to be raw material with the silkworm chrysalis, at 120～190 ℃, and 2.5～30kg/cm
2Be hydrolyzed under the pressure.Hydrolyzed solution is made compound amino acid powder through processes such as degreasing, decolouring, spraying dryings.
The inventive method, technological process is simple, and amino acid is not destroyed, and yield is higher, especially utilizes cocoon chrysalis to do raw material, and its yield is high 10 times than previous methods.
The aminoacids complex that this law is extracted can be used for manufacturing the additive of medical injection of fish and food, feed, daily cosmetics.
Description
A kind of medicine and food of being used as, fodder additives be the extraction method for compound amino acid of raw material with the silkworm chrysalis.
Aminoacids complex is the nutrition agent that current people pay attention to, and the injection of fish made from aminoacids complex is to be used for the active drug that patients such as wound, hepatitis heighten the effect of a treatment.Aminoacids complex is used for nutrient fortified food and feed, can reconcile the nitrogen equilibrium of humans and animals, and its growth is had obvious facilitation.
The extraction of aminoacids complex is to be raw material with the natural protein class.Pharmaceutical arms such as the East Sea, Shanghai pharmaceutical factory are to be raw material with the cruel element of suckling, and are hydrolyzed in the presence of hydrochloric acid and produce.With the silkworm chrysalis is that raw material extraction amino acid is noticeable for a long time research project.Silkworm chrysalis is that the filature of silk textile industry is paid product, utilizes silkworm chrysalis as raw material, is used for industrial production and does not also realize.Pupa albumen is made up of multiple amino acids, is to extract amino acid whose desirable feedstock, and it has cheap, the characteristics of wide material sources.
Just utilize silkworm chrysalis to extract the nutrient protein agent as far back as the forties abroad, China just extracts amino acid to silkworm chrysalis and studies from the fifties, and dandong silk institute utilizes the cocoon chrysalis hydrolysis to extract amino acid." comprehensive utilization of silkworm and mulberry by product " literary composition that south China agricultural college in 1979 delivered in " silkworm and mulberry circular " the special issue third phase has been introduced and has been utilized mulberry silkworm chrysalis to extract amino acid.
Extract amino acid and close alive method for hydrolysis, method for hydrolysis in the past mainly contains acid system hydrolysis, alkali process hydrolysis, enzymatic hydrolysis.These methods are complex process not only, and in hydrolytic process, amino acid there is destruction, for example, milk is cruel plain in hydrolysis and mulberry silkworm chrysalis hydrolysis in the presence of sulfuric acid in the presence of the hydrochloric acid, tryptophane all is damaged, cause when the preparation injection of fish, need to add the straight color propylhomoserin, cost is improved.Remove this, silkworm chrysalis hydrolysis yield is lower, and mulberry silkworm chrysalis can reach about 30%, and cocoon chrysalis is below 5%.
The object of the present invention is to provide a kind of in leaching process amino acid be not damaged, be the aminoacids complex method for making of the high yield of raw material with the silkworm chrysalis.
Method of the present invention is to be raw material with silkworm chrysalis or pupa powder, under high temperature, high pressure, be hydrolyzed, hydrolyzed solution by usual method handle, drying produces aminoacids complex.
High-temperature high-pressure hydrolysis of the present invention, hydrolyzed solution have following 18 seed amino acids after measured:
mg/ml
Threonine 0.103 aspartic acid 0.283
Xie Ansuan 0.280 Serine 0.071
Methionine(Met) 0.014 L-glutamic acid 0.733
Phenylalanine 0.263 glycine 0.194
Methionin 0.116 L-Ala 0.279
Arginine 0.166 Gelucystine 0.021
Histidine 0.018 Isoleucine 0.172
Leucine 0.312 proline(Pro) 0.107
Tyrosine 0.242 tryptophane 0.102
Be applicable to that silkworm chrysalis of the present invention can be that fresh silkworm chrysalis also can adopt silk reeling pupa; The pupa powder can be pupa powder degreasing or unskimmed.
Method of the present invention, silkworm chrysalis or the pupa powder of pulverizing adds a certain amount of water in advance, at 120～190 ℃, 2.5～30kg/cm
2Following hydrolysis 4-10 hour, to filter then, the filtrate of collecting is through degreasing, decolouring, drying and must the brown powder-like product.
The amount that adds entry during hydrolysis, the general about water ratio of silkworm chrysalis is about 75%, the water yield is that heavy 0.5～2 times of pupa is advisable.The pupa powder is moisture lower, and amount of water is that 5～10 times of pupa grain weight amount are advisable.The water yield is very few, the hydrolyzed solution filtration difficulty.Aminoacids content height in residue, thereby loss is big, the water yield is excessive, and plant factor is low, influences economic benefit.
Hydrolysis temperature and pressure are cruxs of the present invention, and it is all influential to hydrolysis rate and percent hydrolysis, and in certain temperature and pressure scope, hydrolysis rate, percent hydrolysis are to improve along with the raising of temperature, pressure.But weigh from production unit condition and efficient, with 120～190 ℃, 2.5～30kg/cm
2Be advisable.
Aftertreatment can be by usual method, and degreasing can be adopted standing demix or tell grease with the cream-milk separator centrifuging; Decolouring can be adopted materials such as gac, potter's clay, diatomite to decolour with absorption method and slough group ammonia; Dry to adopt spraying drying to be advisable.Also can adopt film drying or additive method drying.
The aminoacids complex that adopts the inventive method to produce can be used for manufacturing injection of fish, oral key element mixture and food, feed, daily cosmetics additive.
Embodiment 1
Silkworm chrysalis adds 0.5 kg of water for 1 kilogram after crushed, is warming up to 120～125 ℃ in autoclave, keeps 2.5kg/cm
2Pressure, hydrolysis 10 hours, cooling then treats that pressure disappears, drive still and take out hydrolyzate, after filtration, standing demix, isolate oil reservoir, in the degreasing hydrolyzed solution, add 2% gac of its weight and 4% potter's clay then, carry out decolouring in 1 hour at 90～100 ℃.Take off the group ammonia treatment, handle after-filtration, filtrate is carried out spraying drying under 100 ℃.Can obtain the aminoacids complex of 18 seed amino acids, yield is the wet pupa restatement of 8.12%()
Embodiment 2
Silkworm chrysalis adds 05 kg of water for 1 kilogram after crushed, at 150～160 ℃, and 10kg/cm
2Hydrolysis is 8 hours under the pressure, handles according to the method for example 1 then, and its yield is 9.3%.
Embodiment 3
Silkworm chrysalis adds 1 kg of water for 1 kilogram after crushed, at 180～190 ℃, and 30kg/cm
2Hydrolysis is 5 hours under the pressure, handles according to the method for example 1 then, and its yield is 10.2%.
Embodiment 4
According to the method for example 1,2 and 3, condition, adopting the pupa powder is raw material, the amount that adds entry was respectively 1: 5, and 1: 5,1: 10(weight).The yield of gained aminoacids complex is respectively 31.2%; 35.1%; 41.4%(is in dry pupa powder)
Claims (2)
1, a kind of manufacture method that is used as medicine, reaches the compounded amino of food, fodder additives is to adopt silkworm chrysalis or pupa powder to be hydrolyzed, and hydrolyzed solution is characterized in that through degreasing, decolouring, drying: silkworm chrysalis or pupa powder add a certain amount of former water.At 120～190 ℃, 2.5～30kg/cm
2Under carry out hydrolysis in 4～10 hours.
2, according to the said method of claim 1, it is characterized in that: the amount that adds entry is 0.5～2 times of silkworm chrysalis weight: 5～10 times of pupa grain weight amount.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN198686104864A CN86104864A (en) | 1986-07-30 | 1986-07-30 | Extraction method for compound amino acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN198686104864A CN86104864A (en) | 1986-07-30 | 1986-07-30 | Extraction method for compound amino acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN86104864A true CN86104864A (en) | 1988-02-10 |
Family
ID=4802629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN198686104864A Pending CN86104864A (en) | 1986-07-30 | 1986-07-30 | Extraction method for compound amino acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN86104864A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1049824C (en) * | 1993-06-30 | 2000-03-01 | 李俊杰 | Anti-senility medicine made of compound amino-acids and trace elements |
| CN1054505C (en) * | 1996-12-11 | 2000-07-19 | 林镇才 | Active constitution cosmetic additive |
| KR20020023866A (en) * | 2001-12-24 | 2002-03-29 | 이성희 | Preparation method of silk amino acid and silk peptide by protein hydrolysis of cocoon and its anticancer and antigenetic effects |
| CN100464647C (en) * | 2001-06-08 | 2009-03-04 | 理化学研究所 | Body temp-raising agent of amino acidds for eating or drinking and for medical use |
-
1986
- 1986-07-30 CN CN198686104864A patent/CN86104864A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1049824C (en) * | 1993-06-30 | 2000-03-01 | 李俊杰 | Anti-senility medicine made of compound amino-acids and trace elements |
| CN1054505C (en) * | 1996-12-11 | 2000-07-19 | 林镇才 | Active constitution cosmetic additive |
| CN100464647C (en) * | 2001-06-08 | 2009-03-04 | 理化学研究所 | Body temp-raising agent of amino acidds for eating or drinking and for medical use |
| KR20020023866A (en) * | 2001-12-24 | 2002-03-29 | 이성희 | Preparation method of silk amino acid and silk peptide by protein hydrolysis of cocoon and its anticancer and antigenetic effects |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| WD01 | Invention patent application deemed withdrawn after publication |