CN1297692A - Animal feed additive and its preparation - Google Patents
Animal feed additive and its preparation Download PDFInfo
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- CN1297692A CN1297692A CN99125260A CN99125260A CN1297692A CN 1297692 A CN1297692 A CN 1297692A CN 99125260 A CN99125260 A CN 99125260A CN 99125260 A CN99125260 A CN 99125260A CN 1297692 A CN1297692 A CN 1297692A
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Abstract
By using artificially cultured housefly larva as material, and through induction, separation to obtain nutritious components and medicinal components and mixing in certain proportion, the animal feed additive is prepared, which contains rich protein, amino acids, fat, antibiotic peptide, agglutinin, lysozyme, chitosan, etc. and has the functions of promoting growth of animal, raising immunity and resisting disease. As one kind of natural preparation and green product, the additive has no toxic side effect, no residue in animal body and no environmental pollution. The simple and advanced production process utilizes the housefly larva resource comprehensively.
Description
The present invention relates to the induced product of insect and extraction of substance animal feed additive and preparation method as effective ingredient, particularly a kind of with common house-fly grub (fly maggot) induced product and extraction of substance as animal feed additive of effective ingredient and preparation method thereof.
Documents and materials confirm, fly maggot is nutritious, contain crude protein 59～65% in the dry, fat 2.6～12%, contain the needed several amino acids of animal in its albumen, and each amino acid content all is higher than fish meal, its TEAA is 2.3 times of fish meal, methionine content is 2.7 times of fish meal, and lysine content is 2.6 times of fish meal.The TEAA of fly maggot dry powder is 43.83%, surpass the reference value 40% that Food and Agriculture Organization/World Health Organization (FAO/WHO) proposes, its TEAA/nonessential amino acid total amount value (E/N) is 0.78, surpasses the reference value 0.6 that FAO/WHO proposes.
Simultaneously, housefly has unique effectively defense mechanism, and it shows extremely strong adaptive capacity to adverse circumstances, even also extensive infectious disease can not take place and death under the condition of artificial feeding.Data has confirmed that the housefly body contains a kind of " antibacterial substance " of strong bactericidal action, and this active material is as long as ten thousand/concentration just is enough to kill the germ of invasion.Moreover, also contain the agglutinin of tool antitumor action in its hemolymph.And also contain a kind of Alcaligenes faecalis in the fly maggot body, multiple germ can be suppressed, and the generation of epidermis and the healing that sore is hindered can be promoted.In addition, also contain 2～4% phosphatide in the housefly body, phosphatide has the protection cell membrane, effects such as disease preventing and treating, so fly maggot also has certain medical value.
Moreover, the contained shitosan of common house-fly grub shell be the unique existence of occurring in nature cation edibility fiber, it have promote cell division with growth, strengthen beneficial bacterium in the intestines, suppress harmful bacterium, reinforced immunological function, reinforcement liver function, physiological effect such as anti-infective.Experiment confirm adds a small amount of shitosan in feed, can improve the result of use of fish meal.And, be made into the aqueous solution with shitosan and can be used as feed adhesive and improve the stabilization time of feed in water.
Both at home and abroad the development and use of fly maggot are had more report in recent years, concentrate on mostly and develop fly maggot and substitute fish meal as feedstuff, though produce market is bigger, but the added value of utilization is lower, has not more given play to the pharmaceutical potential of fly maggot.Patent publication No. be the patent of invention of CN1141130 proposed a kind of utilize fly maggot exploitation for human edible nutritional activities dry powder, for the development and use of fly maggot resource provide a high-level application approach.But be limited to the traditional diet idea of people, it is bigger that the prepared product promotion of this invention is used difficulty, and this invention is owing to adopt without any fly maggot of propagating artificially of inducing processing, and the immunologic mechanism of fly maggot is difficult to transfer, and the composition in the polypide is difficult to reach comprehensively, effectively utilizes.Moreover, this invention not with the centrifugal residue that goes out handle again and prepare shitosan, both wasted resource, more do not given play to the advantage of shitosan.
The producer that produces feed addictive at present both at home and abroad is more, but owing to adopt artificial synthetic chemicals mostly, the product toxic and side effect is big, and is residual many in the body, and environment and people are had certain pollution, and this utmost point is not suitable for the trend of current animal-breeding greenization.Therefore it is very necessary to develop the pure natural fodder additive with certain effect.The present invention adapts to the product of this background just.
The objective of the invention is to overcome the defective of existing animal feed additive, provide that a kind of to select the common house-fly grub (fly maggot) of artificial large-scale production for use be raw material, by dual physics inducement, make in the fly maggot body and roll up antibacterial material, medical substance such as agglutinin and lysozyme, adopt the production technology of system then, with the active ingredient in the fly maggot, be that medicinal ingredient is (as antibacterial peptide, agglutinin, lysozyme, shitosan etc.) and nutritional labeling (as protein, amino acid, lipid etc.) extract to greatest extent, and make the bioactive animal feed additive of tool with active ingredient, in animal feed, add this additive, can reach and promote growth to improve immunity, resist the disease, the effect of nourishing healthy.
Embodiment of the present invention are as follows:
Animal feed additive provided by the invention, it is characterized in that: be raw material with the common house-fly grub, comprise fly maggot nutrition and medicinal component, its nutrition comprises protein, amino acid and lipid, medicinal component comprises agglutinin, antibacterial peptide, lysozyme and shitosan, and is contained in per 100 these animal feed additives of gram:
Protein 50-60 gram
Amino acid 45-55 gram
Lipid 2-7 gram
Agglutinin 3-8 milligram
Antibacterial peptide 5-12 milligram
Lysozyme 5-10 milligram
Shitosan 1-10 gram
The preparation method of animal feed additive provided by the invention is characterized in that: with the common house-fly grub that grows to 3 ages in days is raw material, under room temperature to 80 ℃ condition, separates, extracts its nutrition and medicinal component by following processing step:
1, thermal induction is handled: will grow to 3 age in days fly maggots and handle 15-45min with 45-55 ℃ of hot blast;
2, worm/material separates: with the light separation facilities fly maggot of being induced is separated with feed;
3, stab and induce processing: handle the fly maggot 15～30min that is separated with pin type electric vibrating sieve, to stab the housefly polypide and clean housefly polypide adheres to feed;
4, follow-up cultivation: the housefly polypide that will stab after the processing is cultivated 24～48Hr under the environmental condition of 25 ± 2 ℃ of temperature, humidity 60～70%, stops to cultivate during larvae pupation about 30%, stops the nurturing period forbidding only supplying suitable water for food;
5, broken, homogenate: fly maggot after will stopping to cultivate or first pupa grind and homogenate with colloid mill;
6, centrifugal treating: collect the fly maggot slurries after grinding, carry out centrifugal, separation with supercentrifuge, and collect its filter residue and filtrate respectively;
7, spray-drying: filtrate is carried out spray-drying with drying machine with centrifugal spray make fly maggot dry powder, and fly maggot dry powder is sterilized;
8, extract chitin: the 6th step is obtained filter residue put into stirred autoclave, add the hydrochloric acid of 2～3 times of filter residue gross weights, concentration of hydrochloric acid is 3%, to slough the calcium in the maggot shell, the sodium hydroxide solution that adds 2～3 times of filter residue gross weights again, concentration of sodium hydroxide solution is 10%, to remove the albumen in the maggot shell, puts it into the stirring clay kettle at last, in stirring clay kettle, add filter residue gross weight 3-5 2～3% liquor potassic permanganates doubly, soak bleaching 1 hour, promptly get white chitin solid;
9, preparation shitosan: above-mentioned chitin is immersed in 50% sodium hydroxide solution, is warming up to 100-120 ℃, stirring reaction 1-2 hour, filtering alkali lye, be washed to the pH value for neutral, carry out vacuum drying, promptly make white or slightly have the translucent shitosan of pearly luster;
11, allotment: above-mentioned 7 fly maggot dry powder that make and 9 prepared chitosan are allocated for the ratio of 90-99: 10-1 by weight, and just making of the present invention is the animal feed additive of raw material with the fly maggot.
According to the above mode of production, can produce and be rich in antibacterial material, agglutinin, lysozyme, shitosan and protein, amino acid whose natural animal feed addictive.
Involved shitosan also can directly be bought from market and allocate with fly maggot dry powder among the present invention, so can reduce because of producing the equipment investment that the preparation shitosan brings.
The present invention makes fly maggot increase medical value on high nutrition foundation by the physics inducement method, for the deep level development utilization of fly maggot provides effective way, compares with background technology, and patented technology of the present invention has the special efficacy of following aspect:
1, can obviously improve the growth rate of livestock and poultry and aquatic livestock by the prepared animal feed additive of the present invention
And resistance against diseases.
2, be pure natural preparation by the prepared animal feed additive of the present invention, have no side effect, in the no body
Residual, pollution-free to environment and people, belong to green feed additive.
3, simple, advanced person of preparation method involved in the present invention and actual effect make common house-fly grub (fly maggot) resource obtain comprehensive utilization preferably.
The animal feed additive of the present invention's preparation can reach following index:
1, organoleptic indicator:
Outward appearance: cross 60 orders with top sieve, homogeneous disperses; Color: white or faint yellow; Smell: slightly seafood is distinguished the flavor of.
2, nutritional labeling:
Crude protein accounts for the 50-60% of gross weight; 2～7% of crude fat gross weight, wherein each seed amino acid percentage by weight of constitutive protein matter sees Table 1.
Amino acid content (%) in table 1. feed addictive provided by the invention
| Amino acid | Percentage by weight | Amino acid | Percentage by weight |
| Aspartic acid | ????5.60～7.60 | Isoleucine | ????2.60～3.87 |
| Threonine | ????2.03～3.10 | Leucine | ????4.05～5.70 |
| Serine | ????1.53～2.60 | Tyrosine | ????3.24～5.27 |
| Glutamic acid | ????8.21～10.55 | Phenylalanine | ????3.18～4.90 |
| Glycine | ????2.66～3.84 | Lysine | ????4.40～5.07 |
| Alanine | ????2.50～3.30 | Arginine | ????3.18～3.98 |
| Cystine | ????0.30～0.67 | Histidine | ????1.50～2.10 |
| Valine | ????3.17～3.71 | Proline | ????2.19～3.43 |
| Methionine | ????1.25～2.30 |
3, medicinal ingredient:
Antibacterial peptide 0.05-0.12mg/g; Agglutinin 0.03-0.08mg/g; Lysozyme 0.05-0.10mg/g; Shitosan 1-10%.
4. mineral matter and micronutrient levels: as table 2.
Table 2. feed addictive mineral provided by the invention and micronutrient levels
| Element | Content (%) | Element | Content (mg/kg) |
| Mg Ca P K Na | 1.23～1.45 0.18～0.30 1.70～1.90 1.56～1.86 0.27～0.40 | Fe Mn Zn Cu | 300～450 200～400 180～300 39～60 |
5. sanitary index:
Total number of bacteria＜45/g; Escherichia coli＜3/g; Pathogenic bacteria must not detect.
6. heavy metal index:
Lead＜6mg/kg; Arsenic＜1mg/kg; Cadmium＜1mg/kg; Mercury＜0.1mg/kg.
In each step of dry powder preparation of the present invention, temperature all should remain on below 80 ℃.Because when temperature is higher than 80 ℃, the active ingredient in the fly maggot polypide has ruined possibility.In addition, because " high rhythm " characteristics of maggot grow, thereby between above each production link, must closely connect, to avoid rotten or browning phenomenon occurs.Production between the every batch of additive also must closely connect.
Further describe the present invention below in conjunction with drawings and Examples:
Embodiment 1:
Preparation method with animal feed additive provided by the invention produces animal feed additive, with the fly maggot that grows to 3 ages in days is raw material, under room temperature to 80 ℃, separate, extract the effective ingredient in the fly maggot body with following processing step: nutrition and medicinal component:
1, thermal induction is handled: will grow to 3 age in days fly maggots and handle 30min with 50 ℃ of hot blasts;
2, worm/material separates: with light separation facilities (seperator) fly maggot of being induced is separated with feed;
3, stab and induce processing: the 5kg that separation is obtained is placed on pin type electric vibrating sieve and handles 30min, stabs the purpose that housefly polypide and clean housefly polypide adhere to feed to reach;
4, follow-up cultivation: the housefly polypide that will stab after the processing is cultivated 24Hr under the environmental condition of 25 ℃ of temperature, humidity 70%, stops to cultivate during larvae pupation to about 30%, stops the nurturing period forbidding only supplying suitable water for food;
5, broken, homogenate: fly maggot after will stopping to cultivate or first pupa grind and homogenate with colloid mill;
6, centrifugal treating: the fly maggot slurries after collect grinding are that the supercentrifuge of 3500rmp separates and obtains filter residue and filtrate with rotating speed;
7, spray-drying: above-mentioned filtrate is carried out spray-drying with drying machine with centrifugal spray make fly maggot dry powder, fly maggot dry powder is sterilized;
8, extract chitin: it is that fly maggot maggot shell is put into stirred autoclave that the 6th step is obtained filter residue, add the hydrochloric acid of 2 times of filter residue gross weights, concentration of hydrochloric acid is 3%, to slough the calcium in the maggot shell, the sodium hydroxide solution that adds 2 times of filter residue gross weights again, concentration of sodium hydroxide solution is 10%, to remove the albumen in the maggot shell, puts it into the stirring clay kettle at last, in stirring clay kettle, 3% liquor potassic permanganate that adds 3 times of filter residue gross weights soaks bleaching 1 hour, promptly gets white chitin solid;
9, preparation shitosan: chitin is immersed in 50% sodium hydroxide solution, is warming up to 120 ℃, stirring reaction 1 hour, filtering alkali lye is washed to the pH value for neutral, carries out vacuum drying, promptly makes white or slightly has the translucent shitosan of pearly luster;
10, allotment, packing.The above-mentioned 7 fly maggot dry powder that make and the 9 prepared chitosan ratio in 99: 1 is mixed, mixes well, making of the present invention is the animal feed additive 1.25kg of raw material with the housefly maggot.
The animal feed additive that present embodiment 1 makes, with the fly maggot is raw material, comprises fly maggot nutrition and medicinal component, and its nutrition comprises protein, amino acid and lipid, medicinal component comprises agglutinin, antibacterial peptide, lysozyme and shitosan, and is contained in per 100 these animal feed additives of gram:
Protein 60 grams
Amino acid 50 grams
Lipid 4.5 grams
5 milligrams of agglutinins
12 milligrams of antibacterial peptides
6 milligrams of lysozymes
Shitosan 1 gram
Shitosan involved in the present embodiment can directly be bought from market and be mixed with fly maggot dry powder, so can reduce because of producing to prepare the equipment investment that shitosan brings.
Embodiment 2:
Preparation method with animal feed additive provided by the invention produces animal feed additive, with the housefly maggot that grows to 3 ages in days is raw material, under room temperature to 80 ℃, separate, extract the effective ingredient in the housefly maggot body with following processing step: nutrition and medicinal component:
1, thermal induction is handled: will grow to 3 age in days fly maggots and handle 45min with 45 ℃ of hot blasts;
2, worm/material separates: with light separation facilities (seperator) fly maggot of being induced is separated with feed;
3, stab and induce processing: the 30kg that separation is obtained is placed on pin type electric vibrating sieve and handles 15min, stabs the purpose that housefly polypide and clean housefly polypide adhere to feed to reach;
4, follow-up cultivation: the housefly polypide that will stab after the processing is cultivated 48Hr under the environmental condition of 27 ℃ of temperature, humidity 60%, stops to cultivate during larvae pupation to about 30%, stops the nurturing period forbidding only supplying suitable water for food;
5, broken, homogenate: fly maggot after will stopping to cultivate or first pupa grind and homogenate with colloid mill;
6, centrifugal treating: the fly maggot slurries after collect grinding are that the supercentrifuge of 3500rmp separates and obtains filter residue and filtrate with rotating speed;
7, spray-drying: above-mentioned filtrate is carried out spray-drying with drying machine with centrifugal spray make fly maggot dry powder, fly maggot dry powder is sterilized;
8, extract chitin: it is that fly maggot maggot shell is put into stirred autoclave that the 6th step is obtained filter residue, add the hydrochloric acid of 2.5 times of filter residue gross weights, concentration of hydrochloric acid is 3%, to slough the calcium in the maggot shell, the sodium hydroxide solution that adds 3 times of filter residue gross weights again, concentration of sodium hydroxide solution is 10%, to remove the albumen in the maggot shell, puts it into the stirring clay kettle at last, in stirring clay kettle, 3% liquor potassic permanganate that adds 3.5 times of filter residue gross weights soaks bleaching 1 hour, promptly gets white chitin solid;
9, preparation shitosan: chitin is immersed in 50% sodium hydroxide solution, is warming up to 100 ℃, stirring reaction 1 hour, filtering alkali lye is washed to the pH value for neutral, carries out vacuum drying, promptly makes white or slightly has the translucent shitosan of pearly luster;
10, allotment, packing.The above-mentioned 7 fly maggot dry powder that make and the 9 prepared chitosan ratio in 95: 5 is mixed, mixes well, making of the present invention is the animal feed additive 7.8kg of raw material with the fly maggot.
The animal feed additive that present embodiment 2 makes, with the fly maggot is raw material, comprise fly maggot nutrition and medicinal component, its fly maggot nutrition comprises protein, amino acid and lipid, medicinal component comprises agglutinin, antibacterial peptide, lysozyme and shitosan, and is contained in per 100 these animal feed additives of gram:
Protein 53 grams
Amino acid 50 grams
Lipid 3.5 grams
4 milligrams of agglutinins
5 milligrams of antibacterial peptides
6 milligrams of lysozymes
Shitosan 5 grams
Shitosan involved in the present embodiment can directly be bought from market and be mixed with fly maggot dry powder, so can reduce because of producing to prepare the equipment investment that shitosan brings.
Embodiment 3:
Preparation method with animal feed additive provided by the invention produces animal feed additive, with the fly maggot that grows to 3 ages in days is raw material, under room temperature to 80 ℃, separate, extract the effective ingredient in the housefly maggot body with following processing step: nutrition and medicinal component:
1, thermal induction is handled: will grow to 3 age in days fly maggots and handle 30min with 50 ℃ of hot blasts;
2, worm/material separates: with light separation facilities (seperator) fly maggot of being induced is separated with feed;
3, stab and induce processing: the 100kg that separation is obtained is placed on homemade pin type electric vibrating sieve and handles 25min, stabs the purpose that housefly polypide and clean housefly polypide adhere to feed to reach;
4, follow-up cultivation: the housefly polypide that will stab after the processing is cultivated 36Hr under the environmental condition of 23 ℃ of temperature, humidity 65%, stops to cultivate during larvae pupation to about 30%, stops the nurturing period forbidding only supplying suitable water for food;
5, broken, homogenate: fly maggot after will stopping to cultivate or first pupa grind and homogenate with colloid mill;
6, centrifugal treating: the fly maggot slurries after collect grinding are that the supercentrifuge of 3500rmp separates and obtains filter residue and filtrate with rotating speed;
7, spray-drying: above-mentioned filtrate is carried out spray-drying with drying machine with centrifugal spray make fly maggot dry powder, and fly maggot dry powder is sterilized;
8, extract chitin: it is that fly maggot maggot shell is put into stirred autoclave that the 6th step is obtained filter residue, add the hydrochloric acid of 3 times of filter residue gross weights, concentration of hydrochloric acid is 3%, to slough the calcium in the maggot shell, the sodium hydroxide solution that adds 3 times of filter residue gross weights again, concentration of sodium hydroxide solution is 10%, to remove the albumen in the maggot shell, puts it into the stirring clay kettle at last, in stirring clay kettle, 3% liquor potassic permanganate that adds 4 times of filter residue gross weights soaks bleaching 1 hour, promptly gets white chitin solid;
9, preparation shitosan: chitin is immersed in 50% sodium hydroxide solution, is warming up to 110 ℃, stirring reaction 1.5 hours, filtering alkali lye is washed to pH value for neutral, carries out vacuum drying, promptly makes white or slightly has the translucent shitosan of pearly luster;
11, allotment, packing.The above-mentioned 7 fly maggot dry powder that make and the 9 prepared chitosan ratio in 90: 10 is mixed, mixes well, making of the present invention is the animal feed additive 26.5kg of raw material with the fly maggot.
The animal feed additive that present embodiment 3 makes, with the fly maggot is raw material, comprise fly maggot nutrition and medicinal component, its fly maggot nutrition comprises protein, amino acid and lipid, medicinal component comprises agglutinin, antibacterial peptide, lysozyme and shitosan, and is contained in per 100 these animal feed additives of gram:
Protein 50 grams
Amino acid 45 grams
Lipid 2 grams
4 milligrams of agglutinins
7 milligrams of antibacterial peptides
5 milligrams of lysozymes
Shitosan 10 grams
Shitosan involved in the present embodiment can directly be bought from market and be mixed with fly maggot dry powder, so can reduce because of producing to prepare the equipment investment that shitosan brings.
Embodiment 4:
Preparation method with animal feed additive provided by the invention produces animal feed additive, with the fly maggot that grows to 3 ages in days is raw material, under room temperature to 80 ℃, separate, extract the effective ingredient in the fly maggot body with following processing step: nutrition and medicinal component:
1, thermal induction is handled: will grow to 3 age in days fly maggots and handle 15min with 55 ℃ of hot blasts;
2, the worm material separates: with light separation facilities (seperator) fly maggot of being induced is separated with feed;
3, stab and induce processing: the 120kg that separation is obtained is placed on pin type electric vibrating sieve and handles 15min, stabs the purpose that housefly polypide and clean housefly polypide adhere to feed to reach;
4, follow-up cultivation: the housefly polypide that will stab after the processing is cultivated 24Hr under the environmental condition of 25 ℃ of temperature, humidity 60%, stops to cultivate during larvae pupation to about 30%, stops the nurturing period stopping only to supply suitable water for food;
5, broken, homogenate: fly maggot after will stopping to cultivate or first pupa grind and homogenate with colloid mill;
6, centrifugal treating: the fly maggot slurries after collect grinding are that the supercentrifuge of 3500rmp separates and obtains filter residue and filtrate with rotating speed;
7, spray-drying: above-mentioned filtrate is carried out spray-drying with drying machine with centrifugal spray make fly maggot dry powder, fly maggot dry powder is sterilized;
8, extract chitin: it is that fly maggot maggot shell is put into stirred autoclave that the 6th step is obtained filter residue, add the hydrochloric acid of 2 times of filter residue gross weights, concentration of hydrochloric acid is 3%, to slough the calcium in the maggot shell, the sodium hydroxide solution that adds 2 times of filter residue gross weights again, concentration of sodium hydroxide solution is 10%, to remove the albumen in the maggot shell, puts it into the stirring clay kettle at last, in stirring clay kettle, 3% liquor potassic permanganate that adds 5 times of filter residue gross weights soaks bleaching 1 hour, promptly gets white chitin solid;
9, preparation shitosan: chitin is immersed in 50% sodium hydroxide solution, is warming up to 100 ℃, stirring reaction 2 hours, filtering alkali lye is washed to the pH value for neutral, carries out vacuum drying, promptly makes white or slightly has the translucent shitosan of pearly luster;
10, allotment, packing.The above-mentioned 7 fly maggot dry powder that make and the 9 prepared chitosan ratio in 97: 3 is mixed, mixes well, making of the present invention is the animal feed additive 125kg of raw material with the fly maggot.
The animal feed additive that present embodiment 4 makes, with the fly maggot is raw material, comprise fly maggot nutrition and medicinal component, its fly maggot nutrition comprises protein, amino acid and lipid, medicinal component comprises agglutinin, antibacterial peptide, lysozyme and shitosan, and is contained in per 100 these animal feed additives of gram:
Protein 54 grams
Amino acid 55 grams
Lipid 7 grams
8 milligrams of agglutinins
5 milligrams of antibacterial peptides
10 milligrams of lysozymes
Shitosan 3 grams
Shitosan involved in the present embodiment can directly be bought from market and be mixed with fly maggot dry powder, so can reduce because of producing to prepare the equipment investment that shitosan brings.
Raise 30 laying hens with the animal feed additive that the foregoing description 1,2,3,4 and 5 makes, add every day by every chicken and to feed this animal feed additive of 10g, after feeding 100 days, egg production increases by 310 pieces, laying rate improves 11%, and 1 kilogram of egg of every product expends feed and reduces 0.38kg, and chicken is few sick, and survival rate is up to more than 95%.
Raise 5 pigs with the animal feed additive that the foregoing description 1,2,3,4 and 5 makes, add hello this animal feed additive of 50g every day by every pig, feed after 10 days, the piggy body weight increases by 9.2%, and the every weightening finish of piggy 1kg body weight, cost also descends 15.2%.
The present invention makes fly maggot increase medical value on the basis of high nutrition by certain physics inducement method, for the deep level development utilization of fly maggot provides an effective way. Compare with background technology, patented technology of the present invention has the special efficacy of following aspect:
Use the animal feed additive of the present invention's preparation, can obviously improve growth rate and the resistance against diseases of livestock and poultry and aquatic livestock.
The animal feed additive of the present invention's preparation is pure natural preparation, has no side effect, and is pollution-free to environment and people without residual in the body, belongs to green feed additive.
Preparation method elder generation involved in the present invention and then tool actual effect can make the common house-fly grub resource obtain preferably comprehensive utilization.
Claims (3)
1. animal feed additive, it is characterized in that: be raw material with the fly maggot, comprise fly maggot nutrition and medicinal component, its fly maggot nutrition comprises protein, amino acid and lipid, medicinal component comprises agglutinin, antibacterial peptide, lysozyme and shitosan, and is contained in per 100 these animal feed additives of gram:
Protein 50-60 gram
Amino acid 45-55 gram
Lipid 2-7 gram
Agglutinin 3-8 milligram
Antibacterial peptide 5-12 milligram
Lysozyme 5-10 milligram
Shitosan 1-10 gram
2. by the preparation method of the described animal feed additive of claim 1, it is characterized in that: with the fly maggot that grows to 3 ages in days is raw material, under room temperature to 80 ℃ condition, separates, extracts its nutrition and medicinal component by following processing step:
1) thermal induction is handled: will grow to 3 age in days fly maggots and handle 15-45min with 45-55 ℃ of hot blast;
2) worm/material separates: with the light separation facilities fly maggot of being induced is separated with feed;
3) stab and induce processing: handle the fly maggot 15～30min that is separated with pin type electric vibrating sieve, to stab the housefly polypide and clean housefly polypide adheres to feed;
4) follow-up cultivation: the housefly polypide that will stab after the processing is cultivated 24～48Hr under the environmental condition of 25 ± 2 ℃ of temperature, humidity 60～70%, stops to cultivate during larvae pupation about 30%, stops the nurturing period forbidding only supplying suitable water for food;
5) broken, homogenate: fly maggot after will stopping to cultivate or first pupa grind and homogenate with colloid mill;
6) centrifugal treating: collect the fly maggot slurries after grinding, carry out centrifugal, separation with supercentrifuge, and collect filter residue and filtrate respectively;
7) spray-drying: filtrate is carried out spray-drying with drying machine with centrifugal spray make fly maggot dry powder, and fly maggot dry powder is sterilized;
8) extract chitin: the 6th step is obtained filter residue put into stirred autoclave, add the hydrochloric acid of 2～3 times of filter residue gross weights, concentration of hydrochloric acid is 3%, to slough the calcium in the maggot shell, the sodium hydroxide solution that adds 2～3 times of filter residue gross weights again, concentration of sodium hydroxide solution is 10%, to remove the albumen in the maggot shell, puts it into the stirring clay kettle at last, in stirring clay kettle, add filter residue gross weight 3-5 2～3% liquor potassic permanganates doubly, soak bleaching 1 hour, promptly get white chitin solid;
9) preparation shitosan: above-mentioned chitin is immersed in 50% sodium hydroxide solution, is warming up to 100-120 ℃, stirring reaction 1-2 hour, filtering alkali lye, be washed to the pH value for neutral, carry out vacuum drying, promptly make white or slightly have the translucent shitosan of pearly luster;
10) allotment: fly maggot dry powder and the 9th shitosan that goes on foot preparation that above-mentioned the 7th step makes are allocated in the ratio of 90-99: 10-1, and just making of the present invention is the animal feed additive of raw material with the fly maggot.
3. by the preparation method of the described animal feed additive of claim 2, it is characterized in that described shitosan directly buys from market and be mixed with fly maggot dry powder.
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| CN99125260A CN1297692A (en) | 1999-12-01 | 1999-12-01 | Animal feed additive and its preparation |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102026555A (en) * | 2008-05-12 | 2011-04-20 | 罗姆股份有限公司 | Feed, method for producing feed and apparatus for injecting larvae |
| CN102150752A (en) * | 2010-12-20 | 2011-08-17 | 上海高科联合生物技术研发有限公司 | Method for preparing antimicrobial feed additive |
| CN102755260A (en) * | 2012-07-20 | 2012-10-31 | 姚春宝 | Female pudendum cleaning health-care fluid and preparation method thereof |
| CN102755258A (en) * | 2012-07-20 | 2012-10-31 | 梁小毛 | Skin cleaning healthcare solution and preparation method thereof |
| CN103734538A (en) * | 2014-01-09 | 2014-04-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of feed capable of protecting liver of GIFT oreochromis niloticus and boosting immunity of GIFT oreochromis niloticus and feeding method of feed |
| CN106432404A (en) * | 2016-10-25 | 2017-02-22 | 蚌埠学院 | Method for separating antibacterial peptides |
| CN109170173A (en) * | 2018-10-17 | 2019-01-11 | 江门鑫肽生物蛋白有限公司 | A kind of insect metabolin additive and its preparation method and application |
| CN113786000A (en) * | 2021-08-28 | 2021-12-14 | 广西贺州农贝贝农牧科技有限公司 | Equipment and process method for producing antibiotic-free laying hen feed by fly maggots |
| KR102393124B1 (en) * | 2021-01-13 | 2022-05-03 | 농업회사법인 (주)한국유용곤충연구소 | Method for preparing fermented insect feed additive comprising Ptecticus tenebrifer and insect chitin, and fermented insect feed additive prepared by the same |
-
1999
- 1999-12-01 CN CN99125260A patent/CN1297692A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102026555A (en) * | 2008-05-12 | 2011-04-20 | 罗姆股份有限公司 | Feed, method for producing feed and apparatus for injecting larvae |
| CN102150752A (en) * | 2010-12-20 | 2011-08-17 | 上海高科联合生物技术研发有限公司 | Method for preparing antimicrobial feed additive |
| CN102755260A (en) * | 2012-07-20 | 2012-10-31 | 姚春宝 | Female pudendum cleaning health-care fluid and preparation method thereof |
| CN102755258A (en) * | 2012-07-20 | 2012-10-31 | 梁小毛 | Skin cleaning healthcare solution and preparation method thereof |
| CN103734538A (en) * | 2014-01-09 | 2014-04-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of feed capable of protecting liver of GIFT oreochromis niloticus and boosting immunity of GIFT oreochromis niloticus and feeding method of feed |
| CN103734538B (en) * | 2014-01-09 | 2015-12-02 | 中国水产科学研究院淡水渔业研究中心 | A kind of gift tilapia protects the liver and the feed formulation method of develop immunitypty and feeding method |
| CN106432404A (en) * | 2016-10-25 | 2017-02-22 | 蚌埠学院 | Method for separating antibacterial peptides |
| CN109170173A (en) * | 2018-10-17 | 2019-01-11 | 江门鑫肽生物蛋白有限公司 | A kind of insect metabolin additive and its preparation method and application |
| KR102393124B1 (en) * | 2021-01-13 | 2022-05-03 | 농업회사법인 (주)한국유용곤충연구소 | Method for preparing fermented insect feed additive comprising Ptecticus tenebrifer and insect chitin, and fermented insect feed additive prepared by the same |
| CN113786000A (en) * | 2021-08-28 | 2021-12-14 | 广西贺州农贝贝农牧科技有限公司 | Equipment and process method for producing antibiotic-free laying hen feed by fly maggots |
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