CN2837851Y - Biological sample vessel with real-time temperature control function - Google Patents
Biological sample vessel with real-time temperature control function Download PDFInfo
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- CN2837851Y CN2837851Y CN 200520077025 CN200520077025U CN2837851Y CN 2837851 Y CN2837851 Y CN 2837851Y CN 200520077025 CN200520077025 CN 200520077025 CN 200520077025 U CN200520077025 U CN 200520077025U CN 2837851 Y CN2837851 Y CN 2837851Y
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Abstract
The utility model provides a biological sample pool with the function of real-time temperature control. The utility model is characterized in that the upper part or the lower part of a sample chamber is provided with a temperature control pool which forms a circulation chamber by an upper and a lower glass sticking pieces of a circular ring; the side face of the circular ring is provided with two holes, a perfusion tube inserted in the two holes extends out of a base and is communicated with a temperature control liquid through a groove at the side face of the base; a probe of a thermocouple thermometer passes through the holes at the side face of the circular ring and is sealed in the circulation chamber; the base is of a flat block with a hole at the center; the bottom part of the hole is left with a step-shaped rim which projects inwards and is used for placing the temperature control pool and the sample chamber; the lateral face is provided with a pair of grooves for placing the perfusion tube; the depth of the grooves ensures that when the temperature control pool and the sample chamber are placed in the base, an upper cover and the base can be in contact closely, and the temperature control pool and the sample chamber in the base can be fixed. The sample pool of the utility model can regulate the temperature of samples in the sample pool through controlling the temperature or the flow rate of the temperature control liquid in the temperature control pool closely contacted with the samples, and the temperature of the temperature control liquid can be measured in real time through the thermocouple thermometer.
Description
Technical field:
The utility model belongs to the Bioexperiment container technical field, is specifically related to the sample cell that Bioexperiment is used.
Background technology:
In some experiments such as biology, chemistry, often need to make some sample cells.Practices well is to process the hole of a circle or other shapes in the middle of the certain thickness silicone rubber plate, covers with slide at the hole upper and lower surface and forms cavity, and the experiment specimen in use places among the cavity." a kind of be easy to install and remove the biological sample pond of cleaning sealing " that China Patent No. 200320110116.8 proposes, this sample cell can only carry out the experiment under the room temperature owing to there is not temperature control equipment, can not experimentize being below or above under the condition of room temperature.In fact, a lot of Bioexperiment need sample to keep specified temp (not necessarily room temperature) in experimentation, perhaps change temperature with experimental design, and existing sample cell can't reach this requirement.
The utility model content:
The utility model proposes a kind of real-time temperature control biological sample pond, in the realization experimentation temperature at sample cell place is controlled in real time and regulated.
This real-time temperature control biological sample pond comprises the sample chamber 4 that places in the base 2, forms sample cavities 7 with packing ring 9 and the slides 8 pasted up and down thereof in the sample chamber 4, and the loam cake 1 that cooperates by screw thread, hidden discount or magnet with base 2; It is characterized in that: on the sample chamber 4 or under be provided with temperature-controlled pool 3, this temperature-controlled pool 3 forms circulation chamber 6 by the sticking up and down slide 5 of annulus 13, annulus 13 sides have two holes, be inserted in the woven hose 12 groove 10 protuberate basic units 2 external communications temperature control liquid by base 2 sides in this two hole, thermocouple thermometer probe 14 holes of passing annulus 12 sides are sealed in the circulation chamber 6; Base 2 is middle porose flat, the inwardly outstanding step-like edge that is used to place temperature-controlled pool 3 and sample chamber 4 is left in the bottom in hole, the side has a pair of groove 10 that is used for placing woven hose 12, when its degree of depth guaranteed that base 2 is put in temperature-controlled pool 3 and sample chamber 4, loam cake 1 can closely contact with base 2 and temperature-controlled pool 3 in the base 2 and sample chamber 4 is fixing.
During use, base 2 is lain in a horizontal plane on the sample stage, sample chamber 4 is placed in the base 2,4 tops, sample chamber (also sample chamber 4 can be placed temperature-controlled pool 3 tops) of again temperature-controlled pool 3 being packed into, woven hose 12 embeds groove 10 and protuberate basic unit 2 outsides, one end is connected with the peristaltic pump of carrying temperature control liquid, the logical container that reclaims the afflux fluid of the other end.Open peristaltic pump,, utilize peristaltic pump to regulate flow velocity and make circulation chamber 6 interior fluid temperatures be stabilized in T to T with the liquid feeding temperature-controlled pool 3 of temperature T
Room temperatureBetween a certain temperature T
0, this moment, sample cavity 7 interior temperature also were T
0: if experiment needs sample temperature T
0Be higher than room temperature, can regulate flow velocity and heating voltage and make circulation chamber 6 interior fluid temperatures be stabilized in the T that is higher than room temperature earlier with the heating of temperature control liquid
0Temperature in the temperature-controlled pool 3 can be at any time by the flow velocity of regulating temperature control liquid and/or heat controlled; The temperature of temperature control liquid can be measured by thermocouple thermometer 11 at any time by being sealed in the probe 14 that directly contacts temperature control liquid in the circulation chamber 6; Circular groove 15 can be set, so that adopt tweezers clamping loam cake 1 to install at the upper surface of loam cake 1.
Because the real-time temperature control biological sample of the utility model pond is provided with temperature-controlled pool 3 in (or under) on the sample chamber 4, temperature or flow velocity by temperature control liquid in control and the temperature-controlled pool 3 that sample closely contacts, the temperature of sample in the adjustable sample cell, and can measure the temperature of temperature control liquid in real time by thermocouple thermometer.
Description of drawings:
Fig. 1 is the structural representation in the real-time temperature control biological sample of the utility model embodiment pond;
Fig. 2 is sample cell loam cake 1 synoptic diagram;
Fig. 3 is base 2 synoptic diagram of sample cell;
Fig. 4 is the structural representation of temperature-controlled pool 3.
Embodiment:
Embodiment 1:
Fig. 1 has provided the structural representation in the real-time temperature control biological sample of present embodiment pond; Fig. 2 and Fig. 3 have provided the synoptic diagram of loam cake 1 and base 2 respectively.Present embodiment adopts nylon to be processed into loam cake 1 and base 2.Loam cake 1: external diameter 40mm, internal diameter 28mm, high 5mm, the side is processed with screw thread, and upper surface is processed with the circular groove 15 of diameter 2mm, dark 2mm, and is convenient with tweezers installation loam cake 1 and base 2 combinations.Base 2: external diameter 43mm, internal diameter 35mm, high 10mm, the high 1mm in bottom stage shape edge, the side is processed with the rectangular recess 10 of wide 5mm, dark 6.5mm, be used for placing the woven hose 12 of temperature-controlled pool 3, base 2 inwalls are processed with screw thread, can combine with loam cake 1, fixedly put into wherein sample chamber 4 and temperature-controlled pool 3.
Fig. 4 has provided the structural representation of the temperature-controlled pool 3 of present embodiment sample cell; Annulus 13 adopts nylon to process: external diameter 33mm, internal diameter 28mm, high 3.5mm; Thermocouple thermometer 11 adopts homemade copper-Opinions-Pictures copper thermocouple thermometers, demarcating the back with K type chromel-alumel standard couple thermometer uses, the circular hole that its probe 14 is 2.5mm from aperture, annulus 13 side stretches in the circulation chamber 6, insert the steel pipe 12 of external diameter 2.5mm, internal diameter 2mm, long 20mm then, interface 703 silica gel sealings; Annulus 13 upper and lower surfaces are stained with slide 5 with silica gel and form circulation chambers 6, and circulation liquid circulates through steel pipe 12 in the chamber 6, flows out from other end steel pipe, and thermocouple thermometer is measured the temperature of liquid in the circulation chamber 6 in real time.Utilize circular silicone rubber plate to do packing ring 9, external diameter 33mm, thick 1mm can glue the sample chamber that slide is made different volumes with packing ring 9 two sides of different inner diameters according to the experiment needs.The slide 5,8 that present embodiment adopts all is the standard cap slide of diameter 32mm, thick 0.07mm.
If test the temperature required room temperature that is higher than, can the liquid of the chamber 3 that circulates be heated.Present embodiment adopts the condenser pipe of glass processing factory manufacturing as heating tube, and resistive heater places pipe in it, and two ends connect the liquid that making alive can heat the outer tube of flowing through with lead.Guan Yiduan silica gel sealing in the heating tube pours into pumping fluid, avoids heating tube to be heated inequality and bursts.
The BT01-100 model peristaltic pump that present embodiment adopts Baoding Lange company to produce, delivery rate is 0.2～10mL/min, and is adjustable continuously, output pressure-bearing 0.1MPa; With the silicone rubber tube of its coupling, internal diameter 1mm, maximum flow～50ml/min.Utilize silicone rubber tube to connect successively to place woven hose 12 and temperature control liquid collection container in the container, heating tube, peristaltic pump, temperature-controlled pool 3 of temperature control liquid.Form negative pressure in the circulation chamber 6 for protection temperature-controlled pool 3 is made, peristaltic pump is preferably between temperature-controlled pool 3 and temperature control liquid collection container.
Utilize the real-time temperature control biological sample pond of present embodiment to carry out the light tweezer and catch single spermatoblast, under different temperatures, measure the experiment of its vigor:
Temperature control equipment has been added in the real-time temperature control biological sample of the utility model pond on traditional biological sample cell basis, by the temperature of the liquid that flows through in control and the circulation chamber that sample closely contacts, control the temperature of sample indoor sample indirectly.Fluid temperature is measured in real time by thermocouple thermometer in the circulation chamber.Thermometer probe is sealed in the circulation chamber, directly contacts circulating liquid.This sample cell can satisfy the requirement that experimentizes under the different temperatures in the Bioexperiment.
Claims (1)
1, a kind of real-time temperature control biological sample pond, comprise the sample chamber (4) that places in the base (2), form sample cavity (7) with packing ring (9) and the slide (8) pasted up and down thereof in the sample chamber (4), and the loam cake (1) that cooperates by screw thread, hidden discount or magnet with base (2); It is characterized in that: on sample chamber (4) or under be provided with temperature-controlled pool (3), this temperature-controlled pool (3) forms circulation chamber (6) by the sticking up and down slide (5) of annulus (13), annulus (13) side has two holes, be inserted in groove (10) protuberate basic unit (2) the external communications temperature control liquid of woven hose (12) by base (2) side in this two hole, the hole that thermocouple thermometer probe (14) passes annulus (12) side is sealed in the circulation chamber (6); Base (2) is middle porose flat, the inwardly outstanding step-like edge that is used to place temperature-controlled pool (3) and sample chamber (4) is left in the bottom in hole, the side has a pair of groove (10) that is used for placing woven hose (12), when its degree of depth guaranteed that base (2) is put in temperature-controlled pool (3) and sample chamber (4), loam cake (1) can closely contact with base (2) and the temperature-controlled pool (3) in the base (2) and sample chamber (4) is fixing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520077025 CN2837851Y (en) | 2005-10-31 | 2005-10-31 | Biological sample vessel with real-time temperature control function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520077025 CN2837851Y (en) | 2005-10-31 | 2005-10-31 | Biological sample vessel with real-time temperature control function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2837851Y true CN2837851Y (en) | 2006-11-15 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520077025 Expired - Fee Related CN2837851Y (en) | 2005-10-31 | 2005-10-31 | Biological sample vessel with real-time temperature control function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2837851Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267766A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Real-time temperature-control micro sample room |
| CN106153436A (en) * | 2016-08-31 | 2016-11-23 | 广州深达生物制品技术有限公司 | A kind of annealing device of slide glass Samples detection |
| WO2017162149A1 (en) * | 2016-03-21 | 2017-09-28 | 广州深达生物制品技术有限公司 | Device and method of processing sample slide |
| WO2017162150A1 (en) * | 2016-03-21 | 2017-09-28 | 广州深达生物制品技术有限公司 | Heat treatment device for slide specimen testing |
| CN109642859A (en) * | 2016-08-12 | 2019-04-16 | 樱花精机株式会社 | Coverslip sticker |
| CN112147174A (en) * | 2020-08-24 | 2020-12-29 | 西安近代化学研究所 | Sample pool for testing vacuum stability of explosives and powders |
-
2005
- 2005-10-31 CN CN 200520077025 patent/CN2837851Y/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267766A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Real-time temperature-control micro sample room |
| WO2017162149A1 (en) * | 2016-03-21 | 2017-09-28 | 广州深达生物制品技术有限公司 | Device and method of processing sample slide |
| WO2017162150A1 (en) * | 2016-03-21 | 2017-09-28 | 广州深达生物制品技术有限公司 | Heat treatment device for slide specimen testing |
| JP2019510243A (en) * | 2016-03-21 | 2019-04-11 | シァメンタレントバイオメディカルテクノロジー カンパニーリミテッドＸｉａｍｅｎ Ｔａｌｅｎｔ Ｂｉｏｍｅｄｉｃａｌ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｍｐａｎｙ， Ｌｔｄ． | Heat treatment device for sample slide detection |
| US10830679B2 (en) | 2016-03-21 | 2020-11-10 | Xiamen Talent Biomedical Technology Company, Ltd. | Device and method for processing slide specimen thereof |
| US11221281B2 (en) | 2016-03-21 | 2022-01-11 | Xiamen Talent Biomedical Technology Company, Ltd. | Heat treatment device for slide specimen testing |
| CN109642859A (en) * | 2016-08-12 | 2019-04-16 | 樱花精机株式会社 | Coverslip sticker |
| CN109642859B (en) * | 2016-08-12 | 2021-05-18 | 樱花精机株式会社 | Cover glass pasting device |
| CN106153436A (en) * | 2016-08-31 | 2016-11-23 | 广州深达生物制品技术有限公司 | A kind of annealing device of slide glass Samples detection |
| CN112147174A (en) * | 2020-08-24 | 2020-12-29 | 西安近代化学研究所 | Sample pool for testing vacuum stability of explosives and powders |
| CN112147174B (en) * | 2020-08-24 | 2023-08-11 | 西安近代化学研究所 | Sample cell for testing vacuum stability of explosives and powders |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |