CN201277972Y - Cell perfusion apparatus used matching with fluorescent microscope - Google Patents
Cell perfusion apparatus used matching with fluorescent microscope Download PDFInfo
- Publication number
- CN201277972Y CN201277972Y CNU2008202002533U CN200820200253U CN201277972Y CN 201277972 Y CN201277972 Y CN 201277972Y CN U2008202002533 U CNU2008202002533 U CN U2008202002533U CN 200820200253 U CN200820200253 U CN 200820200253U CN 201277972 Y CN201277972 Y CN 201277972Y
- Authority
- CN
- China
- Prior art keywords
- cell
- perfusate
- fluorescent microscope
- memotron
- cell perfusion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses a cell perfusion device used in conjunction with a fluorescent microscope, comprising a peristaltic pump, the fluorescent microscope, a temperature controller, a locule in which cells are sealed, at least two perfusate storage tubes, at least two iron stands and at least two electrothermal sleeves. The locule is arranged on the object stage of the fluorescent microscope, and the perfusate outlet of the locule is connected with the peristaltic pump through a connecting tube; and the perfusate storage tubes are fixed on the two iron stands by a fixer, are connected with the perfusate inlet of the locule through a connecting tube and are sleeved in the electrothermal sleeves connected with the temperature controller. The cell perfusion device is convenient to obtain material, can be conveniently assembled based on experiment use, has good cell perfusion effect, convenient application, simple structure, stable performance, low energy consumption and low cost, is durable and is convenient to change components at any time.
Description
Technical field
The utility model relates to a kind of cell perfusion device, especially a kind of cell perfusion device that is used with fluorescent microscope.
Background technology
The cells in vitro culture technique has become one of common experimental method of current scientific research, and impressive progresses such as cell cycle and regulation and control thereof, canceration mechanism, aging, gene expression and regulation and control all be unable to do without this technology.It is under given conditions that cells in vitro is cultivated, and by isolated cells is studied, the result who is obtained has very important meaning to molecular biology, cell biology, biochemical development, thereby provides foundation for disclosing secrets of life.Now, the research of ion channel on the cultured cell in vitro film is also become the hot issue of each subject research, for example studied the aspects such as pathogenesis of pulmonary hypertension.We know opening of ion channel and close the adjusting that is subjected to multiple factor, and the variation of extracellular fluid ion concentration or composition all can opening and closing and exert an influence ion channel.
At present, abroad the commodity of existing similar cell perfusion system occur, and still, several problems below existing: the cell perfusion system price of (1) import is very expensive, and ordinary laboratory is generally not conventional is furnished with.(2) operation of the cell perfusion system of import is comparatively complicated, is difficult for grasping.(3) the cell perfusion system accessory of import is difficult for buying, and damaging as an accessory needs a whole set of instrument to change.
The utility model content
The purpose of this utility model provides a kind of cell perfusion device that is used with fluorescent microscope.
The purpose of this utility model realizes by following technical measures: a kind of cell perfusion device that is used with fluorescent microscope, and it comprises that peristaltic pump, fluorescent microscope, thermostat, envelope have the cell of cell, at least two perfusate memotrons, at least two iron stands and at least two electric heating heating jackets; Described envelope has the cell of cell to place on the objective table of fluorescent microscope, and the liquid outlet of cell is connected with peristaltic pump by connecting pipe; Described perfusate memotron is fixed on the iron stand by fixator, and the perfusate memotron is connected with the inlet of cell by connecting pipe; The perfusate memotron is with the electric heating heating jacket outward, and described electric heating heating jacket is connected with thermostat.
Connecting pipe between the described perfusate memotron is connected by three-way valve, is connected with the inlet of cell by connecting pipe after making the parallel connection of two perfusate memotrons.
Described perfusate memotron is combination in pairs, and the parallel connection of per two perfusate memotrons is one group, also is by having the cell inlet of cell to be connected with envelope after the three-way valve parallel connection between each group.
Described fluorescent microscope is the calcium ion fluorescent microscope.
Principle of the present utility model: open three-way valve, be contained in perfusate in the perfusate memotron and flow into and be positioned over fluorescent microscope objective table the inside envelope and have in the cell of cell, perfusate by peristaltic pump, flows in the waste liquid cylinder after flowing out by cell at last.Use the calcium ion concentration and the variation thereof on the interior basis of analysis software record cell of fluorescent microscope.The flow velocity of the perfusate in this perfusion device is controlled at 0.5～1 milliliter of per minute usually by the rotating speed control of peristaltic pump.
The utility model compared with prior art has the following advantages: draw materials and easy to assembly, need assemble in experiment and get final product; The cell perfusion is effective; It is convenient to use, durable; Simple in structure, stable performance; Low consumption, low cost; Each parts of system can be changed at any time.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment one.
Embodiment
The cell perfusion device that is used with the calcium ion fluorescent microscope shown in Figure 1 is an embodiment of the present utility model, and it comprises that peristaltic pump 1, calcium ion fluorescent microscope 2, thermostat 4, envelope have the cell 3 of cell, four iron stands 10, two electric heating heating jackets 11 and four perfusate memotron 5A, 5B, 5C, 5D; Envelope has the cell 3 of cell to place on the objective table 21 of calcium ion fluorescent microscope, and the liquid outlet of cell 3 is connected with peristaltic pump 1 by connecting pipe 8; Four iron stands 10 in a row side by side, four perfusate memotrons 5 are fixed on the fixed bar of iron stand 10 with fixator 6 respectively.Connecting pipe 8 usefulness three-way valves under the liquid outlet of perfusate memotron 5A and 5B are connected, and make perfusate memotron 5A, 5B and are unified into one group; Perfusate memotron 5C, 5D are by same mode and be unified into one group, promptly four perfusate memotrons are divided into two groups, and the connecting pipe 8 usefulness three-way valves 7 between two groups of perfusate memotrons are connected on the connecting pipe 8 that is connected with cell 3 inlets that seal cell after being connected in parallel again.
During use, by above-mentioned connected mode assembling cell perfusion system, open and debugged calcium ion fluorescent microscope 2 then earlier, in perfusate memotron 5A, 5B, add and prepared different perfusate A, B, open thermostat 4 and make the perfusate temperature be elevated to 37 degrees centigrade can to experimentize.Open three-way valve 7 successively, after the outlet valve of opening perfusate memotron 5A again makes perfusate A be full of envelope the cell of cell is arranged, open peristaltic pump 1 control flow velocity at per minute 0.5-1 milliliter.Use the calcium ion concentration on the interior basis of analysis software record cell of calcium ion fluorescent microscope 2 simultaneously.Close perfusate memotron 5A valve, open perfusate memotron 5B valve again, perfusate B is flowed out, write down the variation of intracellular calcium concentration again with the analysis software of calcium ion fluorescent microscope 2.Open perfusate memotron 5A at last once more, use perfusate A flushing cell to make intracellular calcium concentration be returned to initial level, continuing experiment as need can add perfusate in perfusate memotron 5C and 5D, carry out same operation and carry out the cell perfusion experiment.
Claims (4)
1, the cell perfusion device that is used of a kind of and fluorescent microscope is characterized in that: it comprises that peristaltic pump, calcium ion fluorescent microscope, envelope have the cell of cell, at least two perfusate memotrons, at least two iron stands, thermostat and at least two electric heating heating jackets; Described envelope has the cell of cell to place on the objective table of calcium ion fluorescent microscope, and the liquid outlet of cell is connected with peristaltic pump by connecting pipe; Described perfusate memotron is fixed on the iron stand by fixator, and the perfusate memotron is connected with the inlet of cell by connecting pipe; The perfusate memotron is with the electric heating heating jacket outward, and described electric heating heating jacket is connected with thermostat.
2, the cell perfusion device that is used of according to claim 1 and fluorescent microscope, it is characterized in that: the connecting pipe between the described perfusate memotron is connected by three-way valve, is connected with the inlet of cell by connecting pipe after the parallel connection of two perfusate memotrons.
3, the cell perfusion device that is used of according to claim 1 and 2 and fluorescent microscope, it is characterized in that: described perfusate memotron is combination in pairs, the parallel connection of per two perfusate memotrons is one group, each the group between by having the cell inlet of cell to be connected with envelope after the three-way valve parallel connection.
4, the cell perfusion device that is used of according to claim 1 and fluorescent microscope, it is characterized in that: described fluorescent microscope is the calcium ion fluorescent microscope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008202002533U CN201277972Y (en) | 2008-09-09 | 2008-09-09 | Cell perfusion apparatus used matching with fluorescent microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008202002533U CN201277972Y (en) | 2008-09-09 | 2008-09-09 | Cell perfusion apparatus used matching with fluorescent microscope |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201277972Y true CN201277972Y (en) | 2009-07-22 |
Family
ID=40895557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008202002533U Expired - Fee Related CN201277972Y (en) | 2008-09-09 | 2008-09-09 | Cell perfusion apparatus used matching with fluorescent microscope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201277972Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102809593A (en) * | 2012-08-15 | 2012-12-05 | 西安交通大学 | Method and device for measuring or monitoring tissue or cell transmembrane potential changes |
| CN105018332A (en) * | 2015-07-17 | 2015-11-04 | 徐州医学院 | Cell perfusion separation device |
| CN105154327A (en) * | 2015-09-30 | 2015-12-16 | 重庆大学 | Blood vessel fluid bionic cell experimental instrument |
| CN105255723A (en) * | 2015-09-30 | 2016-01-20 | 重庆大学 | Using method for blood vessel fluid bionic cell experiment instrument |
-
2008
- 2008-09-09 CN CNU2008202002533U patent/CN201277972Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102809593A (en) * | 2012-08-15 | 2012-12-05 | 西安交通大学 | Method and device for measuring or monitoring tissue or cell transmembrane potential changes |
| CN102809593B (en) * | 2012-08-15 | 2014-07-02 | 西安交通大学 | Method and device for measuring or monitoring tissue or cell transmembrane potential changes |
| CN105018332A (en) * | 2015-07-17 | 2015-11-04 | 徐州医学院 | Cell perfusion separation device |
| CN105154327A (en) * | 2015-09-30 | 2015-12-16 | 重庆大学 | Blood vessel fluid bionic cell experimental instrument |
| CN105255723A (en) * | 2015-09-30 | 2016-01-20 | 重庆大学 | Using method for blood vessel fluid bionic cell experiment instrument |
| CN105255723B (en) * | 2015-09-30 | 2017-09-12 | 重庆大学 | A kind of vascular fluid artificial cell experiment instrument application method |
| US9784662B2 (en) | 2015-09-30 | 2017-10-10 | Chongqing University | Device for vascular hemodynamic bionic cell experiment and methods for using the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201277972Y (en) | Cell perfusion apparatus used matching with fluorescent microscope | |
| CN201003052Y (en) | Simulated pipe biological film test reactor | |
| CN101899391B (en) | Special spectrum airlift photobioreactor | |
| CN201731727U (en) | Experimental saturated wooden antiquity freezing dryer | |
| CN100410139C (en) | Sea thermocline imitation system for test of underwater delivery means | |
| WO2023173495A1 (en) | High-pressure environment marine microorganism enrichment culture and gravity-type isolation apparatus | |
| CN101788452B (en) | Dynamic corrosion test method and equipment thereof | |
| CN202994647U (en) | Multi-channel flow sample pool used for microscope | |
| CN102183397B (en) | Fully-automatic closed type tissue dehydrator | |
| CN207418712U (en) | A kind of biology spirulina cultivation apparatus | |
| CN104560688B (en) | A kind of for cultivating device and the cultural method thereof of mixed microorganism | |
| CN201729835U (en) | Cell constant temperature perfusion device | |
| CN101858844B (en) | Agarose gel micro-flow chamber device for detecting cell migration | |
| Su et al. | An economical device for carbon supplement in large-scale micro-algae production | |
| CN206799614U (en) | A kind of microalgae culturing device adopting of aerostatic buoyancy and GRAVITY CONTROL loop direction | |
| CN207276620U (en) | A kind of energy saving bacterium continuous fermentation tank and its sampler | |
| CN105675864A (en) | Automatic bacteria sorting and marking device based on immune method | |
| CN201555735U (en) | Sample cooler | |
| RU71118U1 (en) | UNIVERSAL AUTOMATED INSTALLATION FOR CULTIVATION OF PHOTO-DEPENDENT MICROORGANISMS | |
| JP4117341B2 (en) | Hot water circulation microscope culture chamber | |
| CN102999066A (en) | Temperature control system | |
| CN203838926U (en) | Temperature control device of variable-temperature viscosity coefficient experimental instrument | |
| CN202995505U (en) | Temperature control system | |
| CN204097465U (en) | A kind of laboratory simple liquid fermentor tank | |
| CN202255904U (en) | Lubricant-fresh water heat exchanger test device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090722 Termination date: 20110909 |