CN2667494Y - Tissue chip micro-array point sample instrument - Google Patents
Tissue chip micro-array point sample instrument Download PDFInfo
- Publication number
- CN2667494Y CN2667494Y CN 200320120918 CN200320120918U CN2667494Y CN 2667494 Y CN2667494 Y CN 2667494Y CN 200320120918 CN200320120918 CN 200320120918 CN 200320120918 U CN200320120918 U CN 200320120918U CN 2667494 Y CN2667494 Y CN 2667494Y
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- China
- Prior art keywords
- axis
- point sample
- muscle platform
- anchor clamps
- band muscle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000002493 microarray Methods 0.000 title claims abstract description 16
- 210000003205 muscle Anatomy 0.000 claims description 36
- 239000004575 stone Substances 0.000 claims description 28
- 230000008520 organization Effects 0.000 claims description 15
- 238000001467 acupuncture Methods 0.000 claims description 8
- 230000004308 accommodation Effects 0.000 claims description 3
- 238000013461 design Methods 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 2
- 239000001993 wax Substances 0.000 description 49
- 239000000523 sample Substances 0.000 description 18
- 210000001519 tissue Anatomy 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000004080 punching Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000005070 sampling Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 208000034189 Sclerosis Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 230000002969 morbid Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
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Abstract
The utility model relates to a tissue chip micro array point sample instrument. A web platform is arranged on a base plate. A Z axes vertical sliding board is arranged on the web platform and is perpendicular to the base plate. An X precision moving device and a Y axes precision moving device are arranged between the web platform and the base plate. A converter is fixed on and perpendicular to the Z axes vertical sliding board. A wax clamp is arranged on the web platform. The face and the back of the web platform are respectively provided with an anchor point and a permanent magnet sheet. A limit knob is arranged between the Z axes vertical sliding board and the web platform. An X axes thousand point knob axes and a Y axes thousand point knob are respectively arranged at the ends of a precise guide rail. The converter is provided with two point sample needle chucks which are respectively provided with a donor needle and a receptor needle. The wax clamp is a trough clamp which is provided with a bolt for fixing a wax block and the back of the clamp is provided with three to five positioning sags. The utility model has the advantages of reasonable design, convenient utilization, handy operation, accurate orientation, easy control and good repeatability.
Description
(1) technical field
The utility model relates to the point sample equipment in a kind of tissue array technology, particularly a kind of microarray point sample instrument.
(2) background technology
Tissue array technology belongs to novel high-tech biotechnology field, and it is that tissue specimen with dozens of even thousands of Different Individual is integrated in institute's formative tissue microarray on the solid phase carrier.Organization chip provides a kind of high flux for Medical Molecular Biology, the multisample and the analysis tool of molecular level fast, it has overcome some defective that exists in traditional pathology method and the biochip technology, makes the mankind can effectively utilize hundreds and thousands of parts of natures or be in tissue specimen under the morbid state to study specific gene and expressed albumen and the correlationship between the disease.
Organization chip then is the situation that research is expressed in thousands of kinds of cell or tissues with a kind of gene or protein molecular, so tissue array technology further developing of traditional pathological technique still not also is the further extension of genetic chip and protein chip.Its advantage: high flux, multisample saves time fast, and easy economy is of many uses, and reliable results be convenient to the contrived experiment contrast, but automated analysis can combine with the other biological technology.And discovery, amplification and expression, the distribution research of protein expression profile in tissue to new gene are significant.
Organization chip microarray point sample instrument is one of most critical equipment in the tissue array technology, and its tissue specimen with dozens of even thousands of Different Individual is integrated in the point sample equipment of formative tissue tissue micro-array chip on the solid phase carrier.Present most organization chip microarray point sample instrument dependence on import, with high costs, for the research work of vast scientific research institutions and medical institutions is made troubles.The development of domestic same category of device still is in the junior stage, and is comparatively backward.
(3) summary of the invention
Deficiencies such as homemade specialized equipment, location are inaccurate at having in the prior art for the utility model, inefficiency, qualification rate are low, a kind of reasonable in design, easy to use, simple operation is provided, accurate positioning, control freely, the organization chip microarray point sample instrument of good reproducibility.
A kind of organization chip microarray point sample instrument, form by main frame and wax stone anchor clamps, main frame comprises base, band muscle platform, Z axle vertical slide, the accurate mobile device of X-axis, the accurate mobile device of Y-axis and converter, band muscle platform is installed on the base, and Z axle vertical slide is installed on the band muscle platform and perpendicular to base; Be used for accommodation zone muscle platform about on the base or the accurate mobile device of the X-axis of front and back position, the accurate mobile device of Y-axis be installed between band muscle platform and the base; Converter is fixed on the Z axle vertical slide and perpendicular to Z axle vertical slide; The wax stone anchor clamps are positioned on the band muscle platform.
The front of band muscle platform is provided with 3-5 anchor point, and its back side is provided with permanent magnets.
Be provided with spacing knob between Z axle vertical slide and the band muscle platform.
The accurate mobile device of accurate mobile device of X-axis and Y-axis returns thousand fens rotation axiss of tight device, big swing handle X-axis by precise guide rail, gap spring respectively and thousand fens knobs of Y-axis are formed, the gap spring is connected with band muscle platform and base respectively, and thousand fens knobs of thousand fens rotation axiss of X-axis and Y-axis are installed in the end of precise guide rail respectively.
Converter is provided with two spotting needle chucks, the donor pin is installed respectively on two spotting needle chucks and is subjected to body acupuncture.
The wax stone anchor clamps are the flute profile anchor clamps, and anchor clamps are provided with the bolt that is used for fixing wax stone, and the back side of anchor clamps is provided with 3-5 location depression.
Use basic operational steps of the present utility model as follows:
1) acceptor wax block is soaked 10min in 39～42 ℃ of water-baths, its softening being convenient to is punched.
2) from water-bath, take out acceptor wax block, lay and be fixed in the wax stone anchor clamps, and be placed on the band muscle platform, the wax stone anchor clamps are near the anchor point of band muscle platform, rapid stationary fixture of magnetic platform and wax stone. with on acceptor wax, punched by body acupuncture, once can make a call to 10 holes continuously as early as possible.When hardening, wax stone stops punching.Otherwise the punching of sclerosis back can destroy the paraffin around the wax hole.
3) take off acceptor wax block, use converter vertically downward, the donor wax stone is fixed on the band muscle platform, with the sampling of donor pin to the donor pin.
4) afterwards, the donor wax stone is taken off, change acceptor wax block, use the donor pin that the sample that takes out is inserted in the acceptor wax hole.
5) from the wax stone anchor clamps, take out wax stone, with the softening again acceptor wax block of the method for step 1).Repeating step 1) to step 4), until the punching of whole acceptor wax block with insert and finish.
When 6) tissue samples is inserted the wax hole, write down the particular location of each sample in two-dimensional array, or design a form consistent (as: row and column uses 1,2 respectively, 3...n and a, b, c.... mark) in advance with acceptor wax hole.The orientation that marks organization chip on this external certain position is (as getting two wax holes and insert sample in the 1st or the 2nd initial row outside.
The utlity model has following characteristics:
1), X, the accurate removable sampling point sample of Y direction system, bearing accuracy can reach 0.01 millimeter;
2), magnetic recording tape muscle platform, guarantee machining precision, guarantee the smooth precision of surface level, magnetic design is that use equipment personnel provide convenience, and can lay freely;
3), multipoint location system, 3-5 anchor point of magnetic recording tape muscle platform, 3-5 anchor point of wax stone anchor clamps guarantees to use the equipment personnel in use, location repeatability is consistent;
4), with spacing Z axle vertical slide, the control sampling point sample degree of depth is with vertical, and the unanimity of the degree of depth;
5), converter, make the donor pin, it is more convenient be used alternatingly by body acupuncture;
6), big thousand fens knobs of handle, good hand touch during use.
Entire machine design is reasonable, easy to use, simple operation, accurate positioning, control freely, the characteristics of good reproducibility, can satisfy the requirement that 1 to 600 organization chip of scientific research needs is made.
(4) description of drawings
Fig. 1 is the utility model structural representation.
Fig. 2 is a vertical view of the present utility model after Fig. 1 rotates 90 °.
Fig. 3 is the fast clamp structure synoptic diagram of wax in the utility model.
Wherein, 1, base, 2, band muscle platform, 3, anchor point, 4, spacing knob, 5, Z axle vertical slide, 6, converter, 7, the spotting needle chuck, 8, the Y-axis gap returns tight device, 9, thousand fens knobs of Y-axis, 10, permanent magnets, 11, thousand fens knobs of X-axis, 12, tight device is returned in the X-axis gap, and 13, precise guide rail, 14, the wax stone anchor clamps, 15, bolt, 16, location depression.
(5) embodiment
Embodiment: structure of the present utility model such as Fig. 1, Fig. 2 and shown in Figure 3, form by main frame and wax stone anchor clamps, main frame comprises base 1, band muscle platform 2, Z axle vertical slide 5, the accurate mobile device of X-axis, the accurate mobile device of Y-axis and converter 6, band muscle platform 2 is installed on the base 1, and Z axle vertical slide 5 is installed on the band muscle platform 2 and perpendicular to base 1; Be used for accommodation zone muscle platform 2 about on the base or the accurate mobile device of the X of front and back position, the accurate mobile device of Y-axis be installed between band muscle platform 2 and the base 1; Converter 6 is fixed on the Z axle vertical slide 5 and perpendicular to Z axle vertical slide 5; Wax stone anchor clamps 14 are positioned on the band muscle platform 2; The front of band muscle platform 2 is provided with 3-5 anchor point 3, and its back side is provided with permanent magnets 10; Be provided with spacing knob 4 between Z axle vertical slide 5 and the band muscle platform 2; The accurate mobile device of accurate mobile device of X-axis and Y-axis returns thousand fens rotation axiss 11 of tight device 8 and 12, big swing handle X-axis by precise guide rail 13, gap spring respectively and thousand fens knobs 9 of Y-axis are formed, the gap is returned tight device 8 and is connected with band muscle platform 2 and base 1 respectively with 12, and thousand fens knobs 9 of thousand fens rotation axiss 11 of X-axis and Y-axis are installed in the end of precise guide rail 13 respectively; Converter 6 is provided with to be installed the donor pin respectively and is subjected to body acupuncture on 7, two spotting needle chucks of two spotting needle chucks; Wax stone anchor clamps 14 are the flute profile anchor clamps, and anchor clamps are provided with the bolt 15 that is used for fixing wax stone, and the back side of anchor clamps is provided with 3-5 location depression 16.
During use, the preparation fusing point is 57 ℃～59 ℃ earlier, the acceptor wax block of suitable size.Donor and acceptor wax block are separately fixed in the wax stone anchor clamps 14.With donor pin and be installed in respectively in the probe chuck 7 of converter 6 by body acupuncture, the donor pin is fixed in the right probe chuck 7 of converter 6, be used for taking a sample at pathology or biological sample wax embedding block. be fixed in the left probe chuck 7 of converter 6 by body acupuncture, be used for acceptor wax block and punch.Acceptor wax block soaks 10min in 39～42 ℃ of water-baths, its softening being convenient to is punched.Take out acceptor wax block from water-bath, lay and be fixed in the wax stone anchor clamps 14, and be placed on the band muscle platform 2, wax stone anchor clamps 14 are near the anchor point of band muscle platform 2, rapid stationary fixture of magnetic platform and wax stone.With on acceptor wax, punched by body acupuncture, once can make a call to 10 holes continuously as early as possible.When hardening, wax stone stops punching.Otherwise the punching of sclerosis back can destroy the paraffin around the wax hole.Take off acceptor wax block, use converter 6 vertically downward, the donor wax stone is fixed on the band muscle platform 2, with the sampling of donor pin to the donor pin.Afterwards, the donor wax stone is taken off, change acceptor wax block, use the donor pin that the sample that takes out is inserted in the acceptor wax hole.
Claims (6)
1, a kind of organization chip microarray point sample instrument, form by main frame and wax stone anchor clamps, main frame comprises base, band muscle platform, Z axle vertical slide, the accurate mobile device of X-axis, the accurate mobile device of Y-axis and converter, it is characterized in that, band muscle platform is installed on the base, and Z axle vertical slide is installed on the band muscle platform and perpendicular to base; Be used for accommodation zone muscle platform about on the base or the accurate mobile device of the X of front and back position, the accurate mobile device of Y-axis be installed between band muscle platform and the base; Converter is fixed on the Z axle vertical slide and perpendicular to Z axle vertical slide; The wax stone anchor clamps are positioned on the band muscle platform.
2, organization chip microarray point sample instrument as claimed in claim is characterized in that, the front of described band muscle platform is provided with 3-5 anchor point, and its back side is provided with permanent magnets.
3, organization chip microarray point sample instrument as claimed in claim is characterized in that, is provided with spacing knob between described Z axle vertical slide and the band muscle platform.
4, organization chip microarray point sample instrument as claimed in claim, it is characterized in that, accurate mobile device of described X-axis and the accurate mobile device of Y-axis return thousand fens rotation axiss of tight device, big swing handle X-axis by precise guide rail, gap spring respectively and thousand fens knobs of Y-axis are formed, the gap spring is connected with band muscle platform and base respectively, and thousand fens knobs of thousand fens rotation axiss of X-axis and Y-axis are installed in the end of precise guide rail respectively.
5, organization chip microarray point sample instrument as claimed in claim is characterized in that, described converter is provided with two spotting needle chucks, the donor pin is installed respectively on two spotting needle chucks and is subjected to body acupuncture.
6, organization chip microarray point sample instrument as claimed in claim is characterized in that, described wax stone anchor clamps are the flute profile anchor clamps, and anchor clamps are provided with and are used for fixing the fast bolt of wax, and the back side of anchor clamps is provided with 3-5 location depression.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200320120918 CN2667494Y (en) | 2003-12-24 | 2003-12-24 | Tissue chip micro-array point sample instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200320120918 CN2667494Y (en) | 2003-12-24 | 2003-12-24 | Tissue chip micro-array point sample instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2667494Y true CN2667494Y (en) | 2004-12-29 |
Family
ID=34348213
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200320120918 Expired - Fee Related CN2667494Y (en) | 2003-12-24 | 2003-12-24 | Tissue chip micro-array point sample instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2667494Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102768175A (en) * | 2012-07-30 | 2012-11-07 | 深圳市开立科技有限公司 | Mobile station and blood cell analyzer thereof |
| CN105467077A (en) * | 2015-11-17 | 2016-04-06 | 重庆大学 | Spotting head for producing microarray sensor |
| CN117351077A (en) * | 2023-09-14 | 2024-01-05 | 广东凯普科技智造有限公司 | Visual correction method for dynamic prediction of sample application instrument |
-
2003
- 2003-12-24 CN CN 200320120918 patent/CN2667494Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102768175A (en) * | 2012-07-30 | 2012-11-07 | 深圳市开立科技有限公司 | Mobile station and blood cell analyzer thereof |
| CN102768175B (en) * | 2012-07-30 | 2013-07-31 | 深圳市开立科技有限公司 | Mobile station and blood cell analyzer thereof |
| CN105467077A (en) * | 2015-11-17 | 2016-04-06 | 重庆大学 | Spotting head for producing microarray sensor |
| CN117351077A (en) * | 2023-09-14 | 2024-01-05 | 广东凯普科技智造有限公司 | Visual correction method for dynamic prediction of sample application instrument |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP03 | Change of name, title or address |
Patentee address after: 250061, No. ten, No. 73, Ji'nan, Shandong Patentee address before: 250012 No. 44, Wenhua West Road, Shandong, Ji'nan |
|
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |