CN2606113Y - Closed fluorescent light bioreactor - Google Patents

Closed fluorescent light bioreactor Download PDF

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Publication number
CN2606113Y
CN2606113Y CN 01230843 CN01230843U CN2606113Y CN 2606113 Y CN2606113 Y CN 2606113Y CN 01230843 CN01230843 CN 01230843 CN 01230843 U CN01230843 U CN 01230843U CN 2606113 Y CN2606113 Y CN 2606113Y
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China
Prior art keywords
elastic clip
bioreactor
column
reactor
fluorescent tube
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Expired - Fee Related
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CN 01230843
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Chinese (zh)
Inventor
陈必链
王明兹
黄键
庄惠如
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Fujian Normal University
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Fujian Normal University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/02Means for providing, directing, scattering or concentrating light located outside the reactor

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to a closed fluorescent light bioreactor which belongs to bioreactors. The outer side of a prior reactor is provided with a system of cold white fluorescent light source which comprises a light frame formed by directly welding a circular base and 3-8 columns which are connected with a concave elastic clip through screws together. The light source comprises annular fluorescent tubes or straight fluorescent tubes and is clip on the concave elastic clip. The power cord is in and out from the mouths for entering lines and outgoing lines. The adjustment of the light intensity is achieved by controlling turning on or off. The utility model is easily produced and can easily develop the scale culture of alga cells.

Description

The cold white fluorescence bioreactor of novel closed-type
The utility model relates to bioreactor, belongs to the bio-reactor class, is used for scale and cultivates alga cells.
Bioreactor is divided into closed photo bioreactor and open type biological reactor.Closed photo bioreactor has the higher efficiency of light energy utilization, can carry out continuous or semicontinuous cultivation, can utilize the computer controlled automatic culturing process, keep purebred cultivation, substratum water evaporates amount is little and keep constant salt alkali concn, realize the high-density culture of little algae and obtain higher unit surface or volumetric production.Therefore, since the eighties in 20th century, the progress that closed photo bioreactor is used for little algae is rapid, and Italy, France, Britain, the U.S., Israel, Canada, Singapore, Russia and China etc. carry out the research of the closed photo bioreactor that is used to cultivate little algae.Closed photo bioreactor is divided into tubular type bioreactor, board-like bioreactor and other specific type, as photoconductive fiber bioreactor etc.The tubular type bioreactor is the transparent pipeline that utilizes glass or synthetic glass to make, and produces the mode of algae by carrying out batch production under outside natural light or the source of artificial light condition, and becomes the principal mode of outdoor large scale culturing spirulina very soon.Long-pending in order to reach high daylighting rate and high light transmitting surface, the caliber of the transparent pipeline that glass or synthetic glass are made constantly diminishes, and has adopted narrow caliber, but when high-density culture, still exist frustule inevitably and be subjected to the light inequality, it is not smooth to circulate, factors such as sticking wall and dissolved oxygen accumulation.Board-like bioreactor is mainly made by transparent glass or poly (methyl methacrylate) plate, and it can regulate the suitableeest daylighting direction according to the variation of sun light intensity and incident direction, increases transmittance; Different reactors is thick keeps short light-path by regulating, and guarantees that effective liquid layer fully is subjected to light; Control constant gas speed impels frustule being subjected to eddy current circulation turbulence on the light direction, strengthens luminous energy and absorbs conversion.But board-like bioreactor structure bulky complex, the power consumption height, optical path length is narrow, has limited the development of reactor.Closed photo thing melon device design alternative optical path length can only judge that relevant optimum theory research is very weak according to existing research case experience.Closed photo bioreactor is divided into two classes by its form of accepting light, and a class is to adopt the reactor of for example full spectrum sunlight of external light source, cold white luminescent lamp etc.Algae is subjected to multifactor impacts such as reactor daylighting surface-area, algae liquid layer thickness, frustule concentration to the absorption and the conversion of luminous energy, especially under the natural light condition, temperature and light intensity with region, season, round the clock change too big, have a strong impact on luminous energy is absorbed and the growth of algae, how to control light intensity, temperature is the key of outdoor bioreactor large scale culturing algae.Adopt Optical Fiber Transmission and light dispersion system other reactors as internal light source, because propagation path of light is short, luminous energy with even light intensity is radiated on the interior algae liquid surface of bioreactor as far as possible effectively, efficiency of light energy utilization height, but in the additional cover complex illumination system of inside reactor, the solution of problems such as photo-thermal effect, leakproof insulation makes the structure of reactor complexity, and it is extremely expensive to manufacture cost, is difficult to be applied to little algae and commercially produces.
The purpose of the utility model design is exactly at above-mentioned deficiency, designs a kind of controlled cold white luminescent lamp external light source closed photo bioreactor.
The utility model is achieved through the following technical solutions: in the external side of the glass pot of stirring type bioreactor cold white fluorescence light source system is set.Light source is made up of some (general 3-8 root) annular fluorescent tubes or straight shape fluorescent tube bulb, and fluorescent tube bulb is fixed on the special stainless steel lamp bracket with a screw by spill elastic clip (every root post have 2-8 individual).This lamp bracket is made of round pedestal and some root posts.Realize the adjusting of light intensity by controlling opening or closing of every fluorescent tube bulb.
Below in conjunction with accompanying drawing book and embodiment the utility model is further described.
Fig. 1 is a longitudinal sectional drawing of the present utility model;
Fig. 2 is a lamp bracket three-dimensional structure diagram of the present utility model.
Bio-reactor tank body (1) is the 5L stirring type bioreactor among Fig. 1.The tank skin of bio-reactor is made of transparent material such as glass, and cold white fluorescence light source system is arranged at the external side of glass pot.Cold white fluorescence light source system by garden type pedestal (6) and the fixing column of fluorescent tube bulb (2) directly welding structure become.Garden type pedestal (6) directly is enclosed within on the worktable in reacting tank body (1) outside.Utilize a tired silk (3) that spill elastic clip (5) is fixed on the column (2) on the column 2, each column (2) can be provided with four (general 2-8) spill elastic clips (5), the entrance hole (6) of a fluorescent tube bulb is arranged on the tube wall of each intermediate plate lower position, and standard type annular lamp tube (4) is fixed on the spill elastic clip (5).Every root post (2) the upward quantity of spill elastic clip (5) is decided according to the quantity of fluorescent tube, but the elastic clip on every root post (2) is no less than two.Column 2 is the stainless steel tube of hollow, and its lower end and garden type pedestal (7) are weldingly connected, and garden shape pedestal (7) can adopt stainless steel to make, and the side tube wall has wire hole.Lead goes out respectively with garden type pedestal wire hole by column (2) entrance hole (6) and links to each other with circline (4), circuit controling switch box.By the quantity that this controller can be regulated the Push And Release of fluorescent tube easily, reach the purpose of adjusting light intensity.
Fig. 2 is the lamp bracket three-dimensional structure diagram in the cold white fluorescence light source system, and it is welded by the column (1) of a garden type pedestal (4) and three or more (as there being four among Fig. 2).Be provided with the spill elastic clip (2) of some (2-8) on the column (1).This spill elastic clip (2) is fixed on the column (1) by a screw (3), and can do 90 ° rotation.The quantity of the living intermediate plate of bullet (2) is decided according to the quantity of fluorescent tube, but the elastic clip (2) on every root post (1) is no less than two.An entrance hole (4) is arranged on the tube wall of each intermediate plate lower position,, be used for fixing annular fluorescent tube when elastic clip (2) when being in transverse state.When elastic clip 2 is in vertical state, be used for fixing the straight-pipe fluorescent lamp spot.
When adopting the utility model to cultivate spirulina plalensis, range of light intensities is 1000-6000Lx, and is constant in culture temperature, under the air flow fixed condition, cultivate through 10d, the biomass of spirulina is 1.3-1.7g (dry weight)/L during results, the biomass when being higher than static the cultivation.When adopting the utility model to carry out condition experiment, light intensity is easy to regulation and control, temperature, air flow and mixing speed may command.
When adopting the utility model to cultivate Porphyridium cruentum (Porphyridium), range of light intensities is 1000-6000Lx, and is constant in culture temperature, under mixing speed, the air flow fixed condition, cultivate through 15d, the biomass during results is 1.1-2.0g (dry weight)/L, the biomass when being higher than static the cultivation.
Above-described only is preferred embodiment of the present utility model.Should be understood that; for the ordinary skill of this area, under the prerequisite that does not break away from the design's principle, also can make some distortion and improvement; as add power supply control timing device, garden shape pedestal garden shape pedestal etc. in two, also should be considered as protection domain of the present utility model.
The utility model is easy to make, and light intensity control is convenient, and loading and unloading are simple, and cost of investment is few, is convenient to carry out the alga cells scale and cultivates and the work of culture condition Study on optimized.

Claims (6)

1. the bioreactor that constitutes of the stirring type bioreactor made by glass of a tank skin is characterized in that this reactor is by utilizing screw to be fixed with the column of spill elastic clip and lamp bracket that garden type pedestal directly is welded and being clipped in the outside that the glass tank body is formed and placed to fluorescent tube bulb in the spill elastic clip.
2. reactor according to claim 1 is characterized in that the column of this lamp bracket has 3-8.
3. reactor according to claim 1 is characterized in that being provided with on each column of this lamp bracket 2-8 elastic clip.
4. reactor according to claim 1 is characterized in that each elastic clip on the column of this lamp bracket is fixed with a screw, and can do the rotations of 90 degree.
5. reactor according to claim 1, it is characterized in that the spill elastic clip on the lamp bracket column of this reactor has 2-8, when being transverse state, can be used for the stationary annular fluorescent tube bulb, when the spill elastic clip becomes vertical state, can be used for fixing straight shape fluorescent tube bulb.
6. reactor according to claim 1 is characterized in that the fluorescent tube bulb of this reactor can be made up of the straight ring shape fluorescent tube that is no less than 2, and horizontal or vertical direction directly is clipped on the spill elastic clip.
CN 01230843 2001-07-19 2001-07-19 Closed fluorescent light bioreactor Expired - Fee Related CN2606113Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 01230843 CN2606113Y (en) 2001-07-19 2001-07-19 Closed fluorescent light bioreactor

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Application Number Priority Date Filing Date Title
CN 01230843 CN2606113Y (en) 2001-07-19 2001-07-19 Closed fluorescent light bioreactor

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1298834C (en) * 2004-11-11 2007-02-07 上海交通大学 Large-scale seaweed cell or tissue reactor pre-culturing system
CN102399681A (en) * 2011-11-30 2012-04-04 镇江绿能环保科技有限公司 Cold-cathode tube flat energy-saving lamp light source light bioreactor
CN106872424A (en) * 2017-01-09 2017-06-20 浙江大学 Phytoplankton original position on-line measuring device based on photosynthetic pigments fluorescence

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1298834C (en) * 2004-11-11 2007-02-07 上海交通大学 Large-scale seaweed cell or tissue reactor pre-culturing system
CN102399681A (en) * 2011-11-30 2012-04-04 镇江绿能环保科技有限公司 Cold-cathode tube flat energy-saving lamp light source light bioreactor
CN106872424A (en) * 2017-01-09 2017-06-20 浙江大学 Phytoplankton original position on-line measuring device based on photosynthetic pigments fluorescence
CN106872424B (en) * 2017-01-09 2019-06-18 浙江大学 Phytoplankton original position on-line measuring device based on photosynthetic pigments fluorescence

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