CN213866184U - A built-in light source photobioreactor for little algae high density culture - Google Patents
A built-in light source photobioreactor for little algae high density culture Download PDFInfo
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- CN213866184U CN213866184U CN202022293345.4U CN202022293345U CN213866184U CN 213866184 U CN213866184 U CN 213866184U CN 202022293345 U CN202022293345 U CN 202022293345U CN 213866184 U CN213866184 U CN 213866184U
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Abstract
The utility model belongs to the technical field of microalgae culture, and discloses a built-in light source photobioreactor for microalgae high-density culture, wherein a light trough is arranged in a PBR shell, and a fluorescent lamp tube is arranged in the light trough; the upper end and the lower end of the PBR shell are respectively connected with a flange in a sealing way; a bearing layer is clamped in the PBR shell close to the lower end, and an aeration stone is arranged on the lower side of the bearing layer; one side of the PBR shell is uniformly provided with 4 liquid taking ports from top to bottom, and spherical valves are fixed on the liquid taking ports; an aeration port is arranged at the lower side of the liquid taking port, and a water inlet is arranged at the lower end of the other side of the PBR shell. The flanges are all provided with screws, and the adjacent screws are 90 degrees; two exhaust ports are arranged in the middle of the upper end flange. The aeration stone is hermetically connected with the aeration port through a pvc pipe. The bearing layer is provided with a plurality of through holes, and the diameter of each through hole is 3 mm. The utility model discloses little algae high density culture later stage still can high-efficiently utilize the light energy, has promoted little algae and has lasted, increase fast.
Description
Technical Field
The utility model belongs to the technical field of little algae is cultivateed, especially, relate to a built-in light source photobioreactor for little algae high density is cultivateed.
Background
At present, biomass energy produced by the microalgae biotechnology has the potential of replacing the traditional biomass energy, and can meet the energy required by the development of the future world. The high value-added products produced by utilizing microalgae have great potential in the fields of food, cosmetics, medicines, aquaculture and the like, and the biodiesel produced in large quantity can replace fossil fuels.
The photobiological reactor (PBR) is a main engineering device required for microalgae culture and is also a main factor influencing the large-scale culture of microalgae. Currently, PBR is largely classified into open type and closed type.
The enclosed light PBR has the advantages of stable culture condition, large illumination surface area-to-volume ratio, high culture density, high yield per unit area and the like. The closed PBR is divided into a flat plate type, a pipeline type, a columnar air-lift type, a stirring type fermentation tank, a floating type film bag and the like, wherein the columnar reactor has the advantages of simple structure, easiness in operation, simplicity in cleaning, lower energy consumption and the like, and the air-lift type system improves the utilization efficiency of CO2, does not need stirring in the microalgae culture process, avoids damage caused by mechanical stirring and is widely researched and applied.
Sunlight is used for microalgae culture, the microalgae culture cost can be effectively reduced, but ultraviolet components in the sunlight can cause damage to algae cells, and solar radiation is limited by regions, seasons and culture time, so that an artificial light source is mostly adopted in PBR, and the type of the PBR is mainly a fluorescent lamp.
The columnar airlift PBR has a main problem that as the cell density of algae is increased continuously, the shielding effect formed by microalgae prevents an external light source from entering, and the microalgae culture solution in the middle area of a cylinder cannot be sufficiently illuminated in the later stage of culture, so that the further increase of the cells of the algae is influenced.
Through the above analysis, the problems and defects of the prior art are as follows: in the later period of high-density culture of microalgae, the middle area of the cylinder can not be sufficiently illuminated.
The difficulty in solving the above problems and defects is: under the condition that the optical density of the algae liquid is increased sharply at the later stage of microalgae culture, if the center of a culture system is sufficiently illuminated, on one hand, the diameter of the columnar PBR can be reduced to reduce the light attenuation, and on the other hand, the light intensity can be increased to enable more light quanta to reach the PBR center. The former reduces the diameter and brings the reduction of PBR volume, the latter increases the light intensity and increases the demand of light source quantity and the input of electric energy, both methods increase the energy consumption investment and increase the culture cost of microalgae.
The significance of solving the problems and the defects is as follows: on the premise of not excessively reducing the diameter of the PBR and not additionally increasing the light source and the electric energy input, the aim of microalgae high-density culture is achieved by changing the structure of the PBR, which is very significant in the aspects of increasing the yield of microalgae and reducing the culture cost of the microalgae.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems existing in the prior art, the utility model provides a built-in light source photobioreactor for microalgae high-density culture.
The utility model discloses a realize like this, a built-in light source photobioreactor for little algae high density is cultivateed is provided with:
a PBR housing;
a lamp groove is arranged in the PBR shell, and a fluorescent lamp tube is arranged in the lamp groove; the upper end and the lower end of the PBR shell are respectively connected with a flange in a sealing way; a bearing layer is clamped in the PBR shell at a position close to the lower end, and an aeration stone is arranged on the lower side of the bearing layer;
one side of the PBR shell is uniformly provided with 4 liquid taking ports from top to bottom, and spherical valves are fixed on the liquid taking ports; an aeration port is arranged on the lower side of the liquid taking port, and a water inlet is arranged at the lower end of the other side of the PBR shell.
Further, the flanges are provided with screws, and the adjacent screws are 90 degrees; two exhaust ports are arranged in the middle of the upper end flange.
Further, the aeration stone is hermetically connected with the aeration port through a PVC pipe.
Furthermore, a plurality of through holes are formed in the bearing layer, and the diameter of each through hole is 3 mm.
Furthermore, a silica gel gasket is arranged between the flange and the PBR shell.
Further, a ballast and a power supply are arranged in the lamp groove and are electrically connected with the fluorescent lamp tube.
Combine foretell all technical scheme, the utility model discloses the advantage that possesses and positive effect are:
first, the utility model discloses improved current column airlift formula PBR's structure, established the lamp groove in, changed external light source into built-in light source the utility model discloses an among the column airlift formula PBR, little algae high density is cultivateed the later stage and still can high-efficiently utilize the light energy, has promoted little algae and has lasted, increase fast.
Second, the utility model discloses can promote the temperature of little algae culture solution, need not extra control by temperature change. Under the condition of room temperature at 24 ℃, the temperature of the external light source columnar airlift PBR algae liquid is stabilized at 26.5 +/-0.5 ℃, and the temperature of the internal light source columnar airlift PBR algae liquid is stabilized at 32 +/-0.5 ℃;
thirdly, the utility model is especially suitable for the high-density culture of microalgae fond of high temperature and the culture of microalgae carried out in winter;
fourthly, the utility model discloses can show promotion little algae growth rate and maximum biomass. Culturing until 6 days, the optical density of the columnar airlift PBR scenedesmus obliquus reaches 1.301, the external light source PBR is 1.035 of the control group, and the experimental group is about 1.3 times of the control group; in terms of biomass, the experimental group reached 0.623g/L at day 6, which was about 38% higher than the control group (0.452 g/L). In addition, the optimal harvesting time was day 5 as determined from the growth curve, at which time the optical density of the experimental group was 1.298 and the biomass was 0.609g/L, which were 45.2% and 52.9% higher than those of the control group, respectively.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a photobioreactor with built-in light source for high-density culture of microalgae according to an embodiment of the present invention;
in the figure: 1. a flange; 2. an exhaust port; 3. a screw; 4. a lamp slot; 5. a fluorescent lamp tube; 6. a liquid taking port; 7. a support layer; 8. a water inlet; 9. an aeration opening; 10. and (4) aerating stones.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
To solve the problems existing in the prior art, the utility model provides a built-in light source photobioreactor for microalgae high-density culture, which is described in detail with reference to the attached drawings.
As shown in fig. 1, the photobioreactor with built-in light source for high-density culture of microalgae provided in this embodiment includes: the device comprises a flange 1, an exhaust port 2, screws 3, a lamp groove 4, a fluorescent lamp tube 5, a liquid taking port 6, a bearing layer 7, a water inlet 8, an aeration port 9 and an aeration stone 10.
In the embodiment, the PBR shell is made of a transparent acrylic sheet, the thickness of the acrylic sheet is 10mm, and the effective volume is 4.0L; a lamp groove 4 is arranged in the PBR shell; a fluorescent lamp tube 5 is arranged in the lamp groove 4, a ballast and a power supply are also arranged outside the PBR, and the ballast and the power supply are electrically connected with the fluorescent lamp tube 5; the fluorescent lamp is of Philips Lifemax series, 30W, 6500K daylight color, and model number TL-D30W/54-765; the rated power of the ballast is 50W, and the power supply is a 220V power line.
The built-in light source PBR is characterized in that the fluorescent lamp tube 5 is built in the columnar airlift PBR, so that on one hand, the proper growth temperature is provided for the algae liquid through the self heating of the light source, on the other hand, the built-in light source can effectively penetrate through the algae liquid in the later stage of microalgae culture, the good light utilization efficiency is achieved, and the problem that the external light source cannot penetrate through the algae liquid to slow down the growth in the later stage of microalgae culture is solved; meanwhile, the lamp groove 4 is internally arranged, the dry and wet separation is realized, the light source is completely separated from the culture solution, and the electric shock risk is avoided.
In the embodiment, the upper end and the lower end of the PBR shell are respectively connected with a flange 1 in a sealing manner, two exhaust ports 2 are arranged in the middle of the upper end flange 1, screws 3 are arranged on the flanges 1, and the adjacent screws 3 are arranged at 90 degrees; seal through flange 1, screw 3 and silica gel gasket, through the fixed action of screw 3, the silica gel gasket in the middle of the flange 1 has played sealed effect under powerful squeezing action to reach isolated air and prevent the mesh that the algae liquid spills over.
In the embodiment, a supporting layer 7 is clamped in the PBR shell at a position close to the lower end, a plurality of through holes are formed in the supporting layer 7, and the diameter of each through hole is 3 mm; the lower side of the supporting layer 7 is provided with an aeration stone 10, and the aeration stone 10 is hermetically connected with the aeration port 9 through a PVC pipe; the aeration work is completed by an aeration stone 10, a PVC pipe, an aeration port 9, an adjustable air pump and a gas flowmeter, the aeration rate is controlled at 0.3VVM, namely the gas flow is controlled at 1.2L/min; the supporting layer 7 is made of a plurality of acrylic plates with the aperture diameter of 3mm, and bubbles can conveniently pass through the supporting layer 7 to supply CO2The purpose of (1).
In the embodiment, 4 liquid taking ports 6 are uniformly arranged on one side of the PBR shell from top to bottom, and spherical valves are fixed on the liquid taking ports 6; the lower end of the other side of the PBR shell is provided with a water inlet 8.
The utility model discloses a theory of operation does: placing the object to be cultured on the upper side of the supporting layer 7 in the PBR shell, during culture, supplying power to the fluorescent lamp tube 5 through the ballast and the power supply to generate light energy, controlling the aeration of the aeration stone 10 through the adjustable air pump, and detecting large gas flow through the gas flow meterSmall, bubbles penetrating the through holes to the upper side of the supporting layer 7 for supplying CO2。
Compared with the conventional external light source PBR, the utility model has better promotion effect on the growth of microalgae. In the aspect of little algae growth temperature, under the ambient condition that the room temperature is 24 ℃, the algae liquid temperature of conventional external light source PBR is stable at 26.5 +/-0.5 ℃, and the utility model discloses PBR algae liquid temperature is stable at 32 +/-0.5 ℃, consequently, the utility model discloses be particularly useful for the cultivation of the high density of the happy high temperature little algae and the little algae cultivation of going on in cold circumstance.
In the aspect of microalgae growth, the scenedesmus obliquus is cultured to the 6 th day, the optical density of the built-in light source columnar airlift PBR reaches 1.301, the external light source PBR is 1.035 of the control group, and the experimental group is about 1.3 times of the control group; on the biomass side, the experimental group reached 0.623 g.L on day 6-1About 0.452 g.L higher than the control group-1)38 percent. The optimum harvest time was day 5, determined from the growth curve, at which time the optical density of the experimental group was 1.298 and the biomass was 0.609 g.L-1Respectively 45.2% and 52.9% higher than the control group; for Spirulina platensis culture, the initial biomass at the time of inoculation was 0.300 g.L-1When the culture is finished, the biomass of the built-in light source PBR group spirulina platensis reaches 3.825 g.L-1The control group was 2.551 g.L-1。
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be covered within the protection scope of the present invention by those skilled in the art within the technical scope of the present invention.
Claims (6)
1. A built-in light source photobioreactor for high-density culture of microalgae is characterized in that the built-in light source photobioreactor for high-density culture of microalgae is provided with:
a PBR housing;
a lamp groove is arranged in the PBR shell, and a fluorescent lamp tube is arranged in the lamp groove; the upper end and the lower end of the PBR shell are respectively connected with a flange in a sealing way; a bearing layer is clamped in the PBR shell at a position close to the lower end, and an aeration stone is arranged on the lower side of the bearing layer;
one side of the PBR shell is uniformly provided with 4 liquid taking ports from top to bottom, and spherical valves are fixed on the liquid taking ports; an aeration port is arranged on the lower side of the liquid taking port, and a water inlet is arranged at the lower end of the other side of the PBR shell.
2. The photobioreactor with built-in light source for high-density culture of microalgae according to claim 1, wherein the flanges are both provided with screws, and the adjacent screws are at an angle of 90 °; two exhaust ports are arranged in the middle of the upper end flange.
3. The photobioreactor with built-in light source for high-density culture of microalgae according to claim 1, wherein the aeration stone is hermetically connected with the aeration port through a PVC pipe.
4. The photobioreactor with built-in light source for high-density culture of microalgae according to claim 1, wherein the supporting layer is provided with a plurality of through holes, and the diameter of the through holes is 3 mm.
5. The photobioreactor with built-in light source for high-density culture of microalgae according to claim 1, wherein a silica gel gasket is arranged between the flange and the PBR shell.
6. The photobioreactor with built-in light source for high-density culture of microalgae according to claim 1, wherein a ballast and a power supply are further arranged outside the PBR, and the ballast and the power supply are electrically connected with the fluorescent lamp tube.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022293345.4U CN213866184U (en) | 2020-10-15 | 2020-10-15 | A built-in light source photobioreactor for little algae high density culture |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022293345.4U CN213866184U (en) | 2020-10-15 | 2020-10-15 | A built-in light source photobioreactor for little algae high density culture |
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| CN213866184U true CN213866184U (en) | 2021-08-03 |
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