CN2574048Y

CN2574048Y - Organic phosphorous pesticide quick tester by disposable immobilized enzyme column

Info

Publication number: CN2574048Y
Application number: CN 02261084
Authority: CN
Inventors: 许学勤; 徐斐; 华泽钊
Original assignee: University of Shanghai for Science and Technology
Current assignee: University of Shanghai for Science and Technology
Priority date: 2002-10-29
Filing date: 2002-10-29
Publication date: 2003-09-17
Anticipated expiration: 2012-10-29

Abstract

The utility model relates to an organic phosphorous pesticide speed tester by a disposable immobilized enzyme column, which is composed of color display liquid, a buffer solution container, an infusion unit, a test enzyme column unit, a delaying pipe unit, a detection unit, and an output unit for control and result, wherein the fluid infusion unit, the test enzyme column unit, the delaying pipe unit, and the detection unit are connected via flexible corrosion resistance rubber pipes from the front side to the back side. Because the utility model uses an immobilized enzyme column, the utility model has the advantages of high sensitivity and stability. The handling technology of the enzyme column has quicker speed than the testing speed of liquid enzyme. By using the measure of pre-suppression reference enzyme column, the false positive rate can be efficiently reduced compared with the existing speed tester.

Description

The organophosphorus pesticide tacheometer of one-time fixing of employing enzyme post

Technical field the utility model relates to the disposable immobilized enzyme column of employing carries out fast detecting to organophosphorus pesticide instrument.

Background technology

How the technology of fast measuring organophosphorus pesticide residue in agricultural product such as fruit, vegetables all detects based on the supression principle of organophosphorus pesticide to certain enzyme (as e cholinesterase che or plant esterase) reaction at present.Occur on the market all utilizing liquid resolvase reaction system to measure at the instrument of organophosphorus pesticide fast detecting.Plurality of reagents need be used in the place that this quasi-instrument comes with some shortcomings, and prepares used apparatus and vessel then and there, and it is still cumbersome partially to detect formality.And the sensitivity of measuring is low, does not reach fixedly maximum residue limit(MRL) standard-required.

Summary of the invention

The purpose of this utility model is the cumbersome and low shortcoming of mensuration sensitivity for the formality that overcomes organophosphorus pesticide fast measuring instrument existence in the market, and a kind of its structure organophosphorus pesticide tacheometer of one-time fixing of the employing enzyme post of fast detecting organophosphorus pesticide at the scene is provided.

The technical solution of the utility model is: it is by the colour developing liquid container, the transfusion unit that buffer container and binary channels peristaltic pump constitute, be equipped with sample enzyme post, the test enzyme pole unit of reference enzyme post and inlet, the time-delay pipe unit, by light source, the detecting unit that extinction chamber and light signal receiving element are formed reaches by power supply, amplifier, analog to digital converter, microprocessor, control that display and data output interface are formed and output unit formation as a result, transfusion unit, the test enzyme pole unit, the time-delay pipe unit, be formed by connecting by the corrosion-resistant sebific duct of soft flexibility before and after between the detecting unit.

Principle of work of the present utility model is as follows: the peristaltic pump of transfusion unit is sent into sample enzyme post and reference enzyme post with damping fluid and colour developing liquid equally with certain flow velocity, processed fruits, the sample liquid of agricultural product such as vegetables is gone into the inlet of enzyme post with syringe, make it enter the enzyme post with damping fluid, under the immobilised enzymes effect of enzyme post, generate product, this product converges the generation chromogenic reaction with colour developing liquid when flowing forward with damping fluid, reactant liquor is behind the time-delay pipe unit, detected by photodetector unit, enzyme post and the different chromogenic reaction that reference enzyme post is produced then can record the organophosphorus pesticide residue amount fast per sample.

Because the utility model adopts immobilized enzyme column, has compared following advantage with the liquid enzyme reaction system:

1. owing on the special carrier material, realize the immobilization of enzyme, can improve Pesticides Testing sensitivity and

Stability.

2, the rapid handling technique of enzyme post can improve than the detection speed of liquid enzyme.

3, adopt the pre-reference enzyme post means that suppress, can more effectively reduce false positive rate by existing tacheometer

Description of drawings

Fig. 1 is that disposable enzyme pillar agricultural chemicals fast detecting instrument constitutes the unit graph of a relation;

Fig. 2 is an enzyme pole unit structural front view;

Fig. 3 is an enzyme pole unit structure vertical view;

Fig. 4 is an enzyme pole unit structure side view;

Fig. 5 is the A-A sectional view of Fig. 2;

Fig. 6 is extinction cell structure figure;

Fig. 7 is an extinction cell structure vertical view;

Fig. 8 is an extinction cell structure side view.

Embodiment

By shown in Figure 1, the organophosphorus pesticide tacheometer of one-time fixing of employing enzyme post comprises colour developing liquid container 1, the transfusion unit 3 that buffer container 2 and binary channels peristaltic pump constitute, be equipped with sample enzyme post 4, the test enzyme pole unit 7 of reference enzyme post 5 and sample liquid inlet 6, time-delay pipe unit 8, by monochromatic source 12, the detecting unit 9 that extinction chamber 13 and light signal receiving element 14 are formed reaches by power supply, amplifier, analog to digital converter, microprocessor, control that display and data output interface are formed and output unit 10. transfusion units 3 as a result, test enzyme pole unit 7, time-delay pipe unit 8, be formed by connecting by the corrosion-resistant sebific duct 11 of soft flexibility before and after between the detecting unit 9.

By Fig. 2～shown in Figure 5, enzyme pole unit 7 is made of enzyme pylon and disposable enzyme post 4,5.The enzyme pylon is by up and down two enzyme post clamping plate 18,19 and spring pilot pin 16 and two backstays 17 constitute.Enzyme post clamping plate respectively have two enzyme post undertaking mouths, and enzyme post undertaking mouth is connected by the hose adapter of silica gel hose with the clamping plate reverse side respectively.Sample introduction threeway 20 is used for sample injection and working fluid shunting.Two enzyme post clamping plate 18,19 are in the same place by spring pilot pin 16 and two backstays, 17 strings, and train wheel bridge 18 can move up and down within the specific limits.The sealing load of enzyme post and enzyme post undertaking mouth is provided by the spring 15 of string on spring pilot pin 16.By shown in Figure 1, the time-delay pipe unit 8 that enzyme pole unit 7 is connected is made of two isometric time-delay flexible pipes, and respectively going here and there at the front end of pipe has threeway, links to each other with the color solution pipe with post enzyme effluent pipe.

Shown in Fig. 1, Fig. 6～8, detecting unit 9 is made of 12 two mobile extinctions of monochromatic source chamber 13 and two light signal receiving elements 14.Monochromatic source can add the color filter combination by ordinary light source and form, also semiconductor monochromatic light element, power supply is provided by control and output unit 10 as a result, and two mobile extinction chambers are made of two parallel glass sheets 24, plastic frame 22, packing ring 25 and holding screw 26 respectively.The lower end of plastic frame is equipped with an inflow liquid hose connection 27 that is connected with the time-delay pipe, and the upper end is equipped with the effluent of guiding into outside the casing and is discarded liquid hose connection 21, and the both sides of plastic frame are equipped with and compress frame 23.But light signal receiving element photoelectric tube, also light resistance receives photosignal and is sent to the control display unit.

Control and output unit as a result by power supply, signal amplification, analog to digital conversion, microprocessor, operating knob, instrument state indication and as a result output constitute.Power unit is other part power supplies of transfusion unit, monochromatic light unit and this unit.Action button mainly contains power switch, infusion pump switch adjusting knob, signals collecting start button, signal terminating time adjusting knob.The instrument state indicating section is a pilot lamp.Output can have following three kinds of forms as a result: 1, color shows, represents yin and yang attribute respectively by two kinds of different colours; 2, numeral shows, specifically with numeral pesticide concentration ppm units), 3, data output interface, can be connected with computing machine, printer etc.

This agricultural chemicals tacheometer operation steps is as follows:

After the power connection, detector enters warm.Stable back instrument state pilot lamp shows steady state (SS), just can enter test operating procedure.

1, press the infusion pump switch, damping fluid and colour developing liquid flow into the pipeline of enzyme pole unit 7 front and back respectively with certain flow rate under the conveying of the binary channels peristaltic pump of transfusion unit 3, converged follow-up afterflow unit 9 after testing before delay unit 8, discharge outside the instrument at last.

2, etc. damping fluid-colour developing liquid mixed liquor flows out from instrument, and the sample liquid that pre-service is good makes it enter the enzyme post with damping fluid with the injection port that syringe injects the enzyme post, generates product under the immobilised enzymes effect of enzyme post.This product flows forward with damping fluid and colour developing liquid converges.Colour developing generation chromogenic reaction in product in the damping fluid and the colour developing liquid.Chromogenic reaction continues to carry out detecting up to detected unit 9 in time-delay pipe unit 8.The reactant liquor of process detecting unit becomes waste liquid and continues to flow forward to be discharged to outside the instrument always.

3, press the signals collecting start button when injecting sample liquid, collected detected signal enters control and output unit 10 as a result, and detection signal is through amplifying, delivering to microprocessor after the analog to digital conversion.Through the deduction reference value, relatively wait calculating with standard value.The result shows with color demonstration, numeral or interface is exported to computing machine or printer etc.

Reference value is the signal value from the reaction of reference enzyme post.What reference enzyme post was used is the enzyme post that agricultural chemicals suppresses inactivation in advance, and sample is through behind this enzyme post, and the color signal that produces during chromogenic reaction is that non-enzyme factor causes in the back.Therefore after deducting this part signal, be the color reaction that the pure product that is produced by the enzyme effect causes.Get rid of the false positive factor with this.Standard value is the signal value of testing under variable concentrations with certain organophosphorus pesticide, the sample signal of deducting the reference signal value with it relatively after, through linear interpolation, can make instrument provide the value that is equivalent to this pesticide concentration.

Claims

1, a kind of organophosphorus pesticide tacheometer of one-time fixing of employing enzyme post, it is characterized in that, it is by colour developing liquid container (1), the transfusion unit (3) that buffer container (2) and binary channels peristaltic pump constitute, be equipped with sample enzyme post (4), the test enzyme pole unit (7) of reference enzyme post (5) and inlet (6), time-delay pipe unit (8), by light source (12), the detecting unit (9) that extinction chamber (13) and light signal receiving element (14) are formed reaches by power supply, amplifier, analog to digital converter, microprocessor, control that display and data output interface are formed and output unit (10) formation as a result, transfusion unit (3), test enzyme pole unit (7), time-delay pipe unit (8), be formed by connecting by the corrosion-resistant sebific duct of soft flexibility (11) before and after between the detecting unit (9).

2, the organophosphorus pesticide tacheometer of one-time fixing of employing enzyme post according to claim 1, it is characterized in that, described test enzyme pole unit (7) is by enzyme pylon and disposable enzyme post (4), (5) constitute, the enzyme pylon is by two enzyme post clamping plate (18) up and down, (19) constitute with spring pilot pin (16) and two backstays (17), enzyme post clamping plate respectively have two enzyme post undertaking mouths, enzyme post undertaking mouth is connected by the hose adapter of silica gel hose with the clamping plate reverse side respectively, enzyme post sample liquid inlet (6) is connected to the sample introduction threeway (20) for sample injection and working fluid shunting usefulness, two enzyme post clamping plate (18), (19) gone here and there together by spring pilot pin (16) and two backstays (17), going up string at spring pilot pin (16) has the spring (15) that enzyme post and enzyme post undertaking mouth sealing load are provided.

3, the organophosphorus pesticide tacheometer of one-time fixing of employing enzyme post according to claim 1 is characterized in that, described detecting unit (9) is made of (12) two mobile extinction chambers of a monochromatic source (13) and two light signal receiving elements (14).

4, according to the organophosphorus pesticide tacheometer of claim 1,4 described one-time fixing of employing enzyme posts, it is characterized in that, described mobile extinction chamber is made of two parallel glass sheets (24), plastic frame (22), packing ring (25) and holding screw (26) respectively, the lower end of plastic frame (22) is equipped with an inflow liquid hose connection (27) that is connected with the time-delay pipe, the upper end is equipped with effluent interface (21), and the both sides of plastic frame are equipped with and compress frame (23).