CN105628882A

CN105628882A - On-site fast detection method for fruit and vegetable pesticide residues

Info

Publication number: CN105628882A
Application number: CN201510978840.XA
Authority: CN
Inventors: 孙莹莹
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-12-24
Filing date: 2015-12-24
Publication date: 2016-06-01

Abstract

The invention discloses an on-site fast detection method for fruit and vegetable pesticide residues. The method comprises the following steps: extracting tool enzymes; immobilizing and packing the tool enzymes into columns; then detecting pesticides in fruits and vegetables; after detection, treating the fruits and the vegetables with a buffering solution, and then performing acid-alkali treatment on the fruits and the vegetables with alkali to recycle carriers of used enzyme columns. The tool enzymes for detection in the on-site fast detection method is low in cost and easy for standardized extraction, so that the cost can be reduced; adopted reference columns are inactivated enzymes of the same type for actual detection of the pesticides in food, so that pigment interference is eliminated, the detection precision is improved, the absolute difference of a detection result does not exceed 6 percent of an arithmetic mean value, and the coincidence rate of a positive result is up to 100 percent; a sample is treated for 7 to 9 minutes and detected for 9 minutes, and is suitable for regular use for multiple times; a technical support is provided for on-site fast detection of the pesticide residues.

Description

A kind of for garden stuff pesticide residue field fast detection method

Technical field

The present invention relates to a kind of for garden stuff pesticide residue field fast detection method, belong to pesticide monitoring technical field.

Background technology

China is pesticide producing, uses big country, and Residual Pesticides in Farm Produce problem is extremely serious. Health and ecological environment are not only constituted grave danger by this, also leverage China's food export trade. The foreign technology barrier that particularly Japanese government is the heaviest since " Positive List System " that in May, 2006 implements makes agricultural products in China meet with entry to WTO impacts, and the agricultural byproducts outlet of nearly 1/3rd is affected. Therefore, ripe effective the Detection Technologies of Pesticide Residues, especially to the residual technology carrying out Fast Monitoring of agriculture, in China, there is extremely wide application prospect. The Site Detection of pesticide residues can quickly obtain the pesticide residues data of fruit and vegetable, for solving the problem such as agricultural product drug residue and drug dependence, carrying out agricultural product quality and safety evaluation and risk analysis, the policies and regulations formulating agricultural product quality and safety supervision provide scientific basis; Trace to the source to manage for carrying out agricultural product quality and safety supervision, enforcement market for farm products access supervision and tracking technical support is provided; Therefore, regularly report and submit to governments at all levels and issue relevant agricultural product quality and safety information with to society, be conducive to improving reputation and the competitiveness of agricultural products in China, ensure food consumption safety and the social stability of the people.

But, the in-situ check and test method existing defects of existing pesticide residues, the toolenzyme price that mainly existing method uses is high, cannot reclaim, cause that testing cost is too high after using, and impact is repeatedly regularly published.

Summary of the invention

For overcoming the deficiencies in the prior art, the invention provides a kind of for garden stuff pesticide residue field fast detection method.

For reaching above-mentioned purpose, the technical solution adopted in the present invention is: a kind of for garden stuff pesticide residue field fast detection method, step is as follows: extracting tool enzyme; To being fixed of toolenzyme, dress post; Then the pesticide in fruit and vegetable is detected; Detect and first processed with buffer solution, then used alkali acid-alkali treatment, to realize the enzyme post after using is carried out carrier recovery.

Further, described toolenzyme is chicken liver-esterase.

Further, the reference column used in described detection uses toolenzyme same kind deactivated enzyme.

The method have the benefit that: the detection toolenzyme of employing is with low cost, be prone to standardization extracts, save cost, the reference column adopted is same kind deactivated enzyme, actual measurement for Pesticide, eliminate pigment interference, improve detection precision, the absolute difference of testing result is less than the 6% of arithmetic mean of instantaneous value, the coincidence rate of positive findings reaches 100%, sample processing time 7-9 minute, the detection time is 9 minutes, is suitable for repeatedly regularly using, provides technical support for pesticide residues field quick detection.

Detailed description of the invention

Below in conjunction with embodiment, the present invention is further elaborated.

Embodiment 1

A kind of method for garden stuff pesticide residue field quick detection, by immobilized enzyme preparation technology, adopts flow injection or direct immersion way that pesticide is detected. Described specifically include following steps for garden stuff pesticide residue field fast detection method:

A, adopt with low cost, be prone to chicken liver-esterase that standardization extracts as the toolenzyme of sensor, extract chicken liver-esterase;

B, use sensor are to being fixed of chicken liver-esterase, dress post;

Pesticide in fruit and vegetable is detected by C, sensor;

D, detect and first processed sensor with buffer solution, then used alkali acid-alkali treatment, to realize the enzyme post after using is carried out carrier recovery.

Said method, the drug residue problem for the maximum fruits and vegetables of common people's current consumption proposes new detection method. Adopt said method, the product pesticide residues situations such as the veterinary antibiotics in production base, wholesale market of agricultural products, the market of farm produce and supermarket are carried out routine testing.

(1) principal item detected

1, greengrocery: Chinese cabbage, Brassica campestris L. ssp.chinensis, Caulis et Folium Ipomoeae aquaticae, Caulis et Folium Brassicae junceae, Caulis et Folium Lactucae Sativae, Broccoli, Fructus Capsici, Fructus Solani melongenae, Fructus Cucumidis sativi, Fructus Momordicae charantiae, Semen vignae sinensis, sweet corn etc.

2, fruits: Fructus Litchi, Arillus Longan, Fructus Musae and mandarin orange etc.

In detection process, perform essentially according to " method of sampling of pesticide residue analysis sample " (NY/T789-2004) and " vegetable pesticide residue detection sampling specification " (NY/T762-2004).

(2) detection project

Qualitative (quickly) detection (fruit and vegerable class): Organophosphorus and carbamate pesticides pesticide residue.

(3) detection method

A kind of method for garden stuff pesticide residue field quick detection.

3, judgment basis and principle: on fruit and vegetable, the quick testing result of Organophosphorus and carbamate pesticides class residual toxicity judges according to computational methods about suppression ratio in " on vegetable Organophosphorus and carbamate pesticides class residual toxicity method for quick " (NY/T448-2001); Pesticide residues detection by quantitative result judges according to " MRL of Pesticide " (GB2763-2005). The whole eligible of detected project, it is determined that by " it is qualified that this product is examined project "; Wherein there is a detection unable to close the eyes lattice person, be namely judged to " this product is defective ".

Sample segment testing result is as follows: table 1 sample detection result table

Note: three Duplicate Samples of each sample detection, makes standard curve with standard dichlorvos solution, and the testing result of other pesticide is all in the dichlorvos of suitable toxicity.

In sum, what the present invention proposed is used for garden stuff pesticide residue field fast detection method, and the detection toolenzyme of employing is chicken liver-esterase with low cost, to be prone to standardization extraction. The reference column adopted is same kind deactivated enzyme, for the actual measurement of Pesticide, eliminates pigment interference, improving detection precision, the absolute difference of testing result is less than the 6% of arithmetic mean of instantaneous value, and the coincidence rate of positive findings reaches 100%, sample processing time 7-9 minute, the detection time is 9 minutes.

Embodiment 2

The toolenzyme adopted in step A can also have the toolenzyme of its characteristic for other except chicken liver-esterase.

Above-described embodiment is explanation of the invention, it is impossible to be illustrative as limitation of the present invention, description of the invention and application, is not wishing to limit the scope of the invention in above-described embodiment. The deformation of embodiments disclosed herein and change are possible, for those skilled in the art embodiment replace and the various parts of equivalence are known. It should be appreciated by the person skilled in the art that when without departing from the spirit of the present invention or substitutive characteristics, the present invention can in other forms, structure, layout, ratio, and realize with other assembly, material and parts. When without departing from the scope of the invention and spirit, it is possible to embodiments disclosed herein is carried out other deformation and changes.

Claims

1., for a garden stuff pesticide residue field fast detection method, step is as follows: extracting tool enzyme; To being fixed of toolenzyme, dress post; Then the pesticide in fruit and vegetable is detected; Detect and first processed with buffer solution, then used alkali acid-alkali treatment, to realize the enzyme post after using is carried out carrier recovery.

2. according to claim 1 for garden stuff pesticide residue field fast detection method, it is characterised in that: described toolenzyme is chicken liver-esterase.

3. according to claim 1 for garden stuff pesticide residue field fast detection method, it is characterised in that: the reference column used in described detection uses toolenzyme same kind deactivated enzyme.