CN102121044A - System and method for rapidly detecting pesticide residues in fruits and vegetables on site - Google Patents
System and method for rapidly detecting pesticide residues in fruits and vegetables on site Download PDFInfo
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- CN102121044A CN102121044A CN201010022537XA CN201010022537A CN102121044A CN 102121044 A CN102121044 A CN 102121044A CN 201010022537X A CN201010022537X A CN 201010022537XA CN 201010022537 A CN201010022537 A CN 201010022537A CN 102121044 A CN102121044 A CN 102121044A
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Abstract
The invention discloses a system and method for rapidly detecting pesticide residues in fruits and vegetables on site. The system comprises at least a sensor, wherein the sensors adopt chicken liver esterase as tool enzyme; the sensors are used for extracting the chicken liver esterase, immobilizing the chicken liver esterase and packing the chicken liver esterase in a column; then the pesticides in the fruits and vegetables are detected with the enzyme column; and after detection, the enzyme column is firstly treated with buffer solution and then treated with acid base to realize carrier recovery of the used enzyme column. The system and the method have the following beneficial effects: the adopted detecting tool enzyme is the chicken liver esterase which has low cost and is easy to extract in a standard manner, so the cost is saved and the tool enzyme is easy to extract; the adopted reference column is homologous deactivated enzyme which is used for actually detecting the pesticides in the food and eliminates pigment interference, thus improving the detection precision; the absolute different value of the detection results does not exceed 6% of the arithmetic mean value; the coincidence rate of the positive results is 100%; the sample treatment time is 7-9 minutes; and the detection time is 9 minutes.
Description
Technical field
The invention belongs to agricultural product quality and safety detection technique field, relate to the on-the-spot detection system of a kind of pesticide residue, relate in particular to a kind of garden stuff pesticide residue field quick detection system that is used for; Simultaneously, the invention still further relates to the detection method of above-mentioned field quick detection system.
Background technology
China be in the world pesticide producing, use big country, the agricultural chemicals residue problem is very serious in the agricultural-food.This not only constitutes grave danger to HUMAN HEALTH and ecotope, has also influenced China's food export trade greatly.Particularly Japanese government's the heaviest foreign technology barrier since " the positive list system " of in May, 2006 enforcement makes agricultural products in China meet with entry to WTO impacts, and nearly 1/3rd agricultural byproducts outlet is affected.Therefore, ripe effectively Detecting Pesticide technology especially to the residual technology of monitoring fast of farming, has extremely wide application prospect in China.
The scene that utilizes advanced science and technology to carry out pesticide residue is detected, obtain the pesticide residue data of fruit and vegetable fast, for solving problems such as agricultural-food drug residue and drug abuse, carry out agricultural product quality and safety evaluation and venture analysis, the policies and regulations of formulating the agricultural product quality and safety supervision provide scientific basis; For carrying out agricultural product quality and safety supervision, implementing the supervision of market for farm products access and the tracking management of tracing to the source technical support is provided; Regularly report and submit and issue relevant agricultural product quality and safety information to society, improve the reputation and the competitive power of agricultural products in China, ensure the people's food consumption safety and social stability to governments at all levels.
Yet the defective of the in-situ check and test method of existing pesticide residue is that the toolenzyme price that existing method is used is high, and toolenzyme can't reclaim after using simultaneously.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of garden stuff pesticide residue field quick detection system that is used for, to solve the deficiencies in the prior art part.
In addition, the present invention also provides the detection method of above-mentioned field quick detection system.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
A kind of garden stuff pesticide residue field quick detection system that is used for, described system comprises at least one transmitter, described transmitter adopts the chicken gizzard esterase as toolenzyme; Described transmitter extracts the chicken gizzard esterase, and the chicken gizzard esterase is carried out immobilization, dress post; Then the agricultural chemicals in the fruit and vegetable is detected; Detected earlier and handled, used the alkali acid-alkali treatment again, to realize that the enzyme post after using is carried out carrier recovery with buffered soln.
As a preferred embodiment of the present invention, described detection system further comprises a server, and this server connects each transmitter, in order to write down the data that each sensor detecting arrives.
As a preferred embodiment of the present invention, described system comprises wireless router, and described server, each transmitter comprise wireless communication unit, in order to exchange information.
A kind of above-mentioned detection method that is used for garden stuff pesticide residue field quick detection system, described method comprises the steps:
A, adopt the toolenzyme of chicken gizzard esterase, extract the chicken gizzard esterase as transmitter;
B, transmitter carry out immobilization, dress post to the chicken gizzard esterase;
C, transmitter detect the agricultural chemicals in the fruit and vegetable;
D, detected earlier and handled, used the alkali acid-alkali treatment again, to realize that the enzyme post after using is carried out carrier recovery with buffered soln.
As a preferred embodiment of the present invention, described method further comprises step e: each transmitter is sent to the data that collect in one server.
As a preferred embodiment of the present invention, the reference column that described method adopts is a same kind deactivated enzyme.
Beneficial effect of the present invention is: the present invention proposes is used for garden stuff pesticide residue field quick detection system and method, the testing tool enzyme of employing be with low cost, be easy to the chicken gizzard esterase that stdn is extracted, save cost, and be easy to extract.The reference column that adopts is a same kind deactivated enzyme, is used for the actual measurement of food agricultural chemicals, has got rid of the pigment interference, improve and detect precision, the absolute difference of detected result is no more than 6% of arithmetical av, and the coincidence rate of positive findings reaches 100%, 7-9 minute sample preparation time, be 9 minutes detection time.
Description of drawings
Fig. 1 is used for the composition synoptic diagram of garden stuff pesticide residue field quick detection system for the present invention.
Fig. 2 is the schema of detection method of the present invention.
Embodiment
Describe the preferred embodiments of the present invention in detail below in conjunction with accompanying drawing.
Embodiment one
The present invention has disclosed a kind of system and method that is used for the garden stuff pesticide residue field quick detection, by immobilized enzyme preparation technology, adopts flow injection or direct immersion way that agricultural chemicals is detected.
See also Fig. 1, in the present embodiment, the present invention is used for garden stuff pesticide residue field quick detection system and comprises server 10, at least one transmitter 20; Server connects each transmitter, in order to write down the data that each sensor detecting arrives.
Described transmitter adopts the chicken gizzard esterase as toolenzyme; Described transmitter extracts the chicken gizzard esterase, and the chicken gizzard esterase is carried out immobilization, dress post; Then the agricultural chemicals in the fruit and vegetable is detected; Detected earlier and handled, used the alkali acid-alkali treatment again, to realize that the enzyme post after using is carried out carrier recovery with buffered soln.
Preferably, described system comprises wireless router, and correspondingly, described server 10, each transmitter 20 comprise wireless communication unit 11,21, in order to exchange information.Certainly, server 10 and each transmitter 20 also can be wired.
More than introduced the garden stuff pesticide residue field quick detection system that is used for of the present invention, the present invention also discloses the above-mentioned detection method that is used for garden stuff pesticide residue field quick detection system when disclosing said detecting system; See also Fig. 2, described detection method comprises the steps:
Steps A, adopt with low cost, be easy to chicken gizzard esterase that stdn extracts toolenzyme as transmitter, extract the chicken gizzard esterase;
Step B, transmitter carry out immobilization, dress post to the chicken gizzard esterase;
Step C, transmitter detect the agricultural chemicals in the fruit and vegetable;
Step D, detected earlier and handled, used the alkali acid-alkali treatment again, to realize that the enzyme post after using is carried out carrier recovery with buffered soln.
Step e, each transmitter are sent to the data that collect in one server.
In addition, the reference column of described method employing is a same kind deactivated enzyme.
Said system and method have proposed new detection method at the drug residue problem of the maximum fruits and vegetables of common people's current consumption.
Adopt aforesaid method, vegetables, the products such as fruit pesticide residue situation in production base, wholesale market of agricultural products, the market of farm produce and supermarket are carried out daily detection.
(1) principal item of Jian Ceing
1, greengrocery: Chinese cabbage, cabbage heart, water spinach, leaf mustard, romaine lettuce, cabbage, capsicum, eggplant, cucumber, balsam pear, cowpea, sweet corn etc.
2, fruits: lichee, longan, banana and citrus etc.
In testing process, mainly carry out according to " method of sampling of pesticide residue analysis sample " (NY/T 789-2004) and " vegetable pesticide residue detects the sampling standard " (NY/T 762-2004).
(2) test item
Qualitative (fast) detects (fruits and vegetables class): organophosphorus and carbamate pesticide residue.
(3) detection method
A kind of method that is used for the garden stuff pesticide residue field quick detection.
3, judgment basis and principle:
Organophosphorus and carbamate chemicals for agriculture residual hazard rapid detection result are according to judging about the method for calculation of inhibiting rate in " organophosphorus and carbamate chemicals for agriculture residual hazard method for quick on the vegetables " (NY/T 448-2001) on the fruit and vegetable; Pesticide residue detection by quantitative result judges according to " maximum residue limit of agricultural chemicals in the food " (GB 2763-2005).The whole eligibles of institute's test item are judged to be " this product institute inspection project is qualified "; A test item failure is wherein arranged, promptly be judged to be " this product is defective ".
The sample segment detected result is as follows:
| Pesticide species | Sample | Amount of reinforcement (mg/kg) | Detected result is (in SD-1750, mg/kg) | The rate of recovery | Remarks |
| SD-1750 Rogor parathion-methyl acephatemet Malathion | Pterocladia tenuis Okam. | 0.50 0.49 0.50 0.49 0.47 | 0.43 0.12 0.12 0.14 0.13 | 86％ / / / / | The sample of Qiang Huaing has not been measured by the farming inspection GC of institute and has not been contained organophosphorus and carbamate chemicals for agriculture |
| The SevinCarbaryl methomyl | Cucumber | 0.52 0.54 | 0.30 0.27 | / / |
Table 1 sample detection is table as a result
Annotate: three parallel samples of each sample detection, make typical curve with standard SD-1750 solution, the detected result of other agricultural chemicals is all in quite toxic SD-1750.
In sum, the present invention proposes is used for the garden stuff pesticide residue field fast detection method, the testing tool enzyme of employing be with low cost, be easy to the chicken gizzard esterase that stdn is extracted.The reference column that adopts is a same kind deactivated enzyme, is used for the actual measurement of food agricultural chemicals, has got rid of the pigment interference, improve and detect precision, the absolute difference of detected result is no more than 6% of arithmetical av, and the coincidence rate of positive findings reaches 100%, 7-9 minute sample preparation time, be 9 minutes detection time.
Embodiment two
In the present embodiment, the toolenzyme that adopts in the steps A can also have the toolenzyme of its characteristic for except that the chicken gizzard esterase other.
Embodiment three
Do not comprise server in the present embodiment, but individual transmitter can work independently also.On the transmitter display unit can be set, in order to show detected result.
Here description of the invention and application is illustrative, is not to want with scope restriction of the present invention in the above-described embodiments.Here the distortion of disclosed embodiment and change are possible, and the various parts of the replacement of embodiment and equivalence are known for those those of ordinary skill in the art.Those skilled in the art are noted that under the situation that does not break away from spirit of the present invention or essential characteristic, and the present invention can be with other form, structure, layout, ratio, and realize with other assembly, material and parts.Under the situation that does not break away from the scope of the invention and spirit, can carry out other distortion and change here to disclosed embodiment.
Claims (6)
1. one kind is used for garden stuff pesticide residue field quick detection system, it is characterized in that described system comprises at least one transmitter, and described transmitter adopts the chicken gizzard esterase as toolenzyme;
Described transmitter extracts the chicken gizzard esterase, and the chicken gizzard esterase is carried out immobilization, dress post; Then the agricultural chemicals in the fruit and vegetable is detected; Detected earlier and handled, used the alkali acid-alkali treatment again, to realize that the enzyme post after using is carried out carrier recovery with buffered soln.
2. the garden stuff pesticide residue field quick detection system that is used for according to claim 1 is characterized in that:
Described detection system further comprises a server, and this server connects each transmitter, in order to write down the data that each sensor detecting arrives.
3. the garden stuff pesticide residue field quick detection system that is used for according to claim 2 is characterized in that:
Described system comprises wireless router, and described server, each transmitter comprise wireless communication unit, in order to exchange information.
4. the described detection method that is used for garden stuff pesticide residue field quick detection system of one of claim 1 to 3 is characterized in that described method comprises the steps:
A, adopt the toolenzyme of chicken gizzard esterase, extract the chicken gizzard esterase as transmitter;
B, transmitter carry out immobilization, dress post to the chicken gizzard esterase;
C, transmitter detect the agricultural chemicals in the fruit and vegetable;
D, detected earlier and handled, used the alkali acid-alkali treatment again, to realize that the enzyme post after using is carried out carrier recovery with buffered soln.
5. detection method according to claim 4 is characterized in that:
Described method further comprises step e: each transmitter is sent to the data that collect in one server.
6. detection method according to claim 4 is characterized in that:
The reference column that described method adopts is a same kind deactivated enzyme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010022537XA CN102121044A (en) | 2010-01-07 | 2010-01-07 | System and method for rapidly detecting pesticide residues in fruits and vegetables on site |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010022537XA CN102121044A (en) | 2010-01-07 | 2010-01-07 | System and method for rapidly detecting pesticide residues in fruits and vegetables on site |
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| CN102121044A true CN102121044A (en) | 2011-07-13 |
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| CN201010022537XA Pending CN102121044A (en) | 2010-01-07 | 2010-01-07 | System and method for rapidly detecting pesticide residues in fruits and vegetables on site |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103513012A (en) * | 2012-06-30 | 2014-01-15 | 句容鑫林农业科技服务有限公司 | Vegetable safety detection system and method |
| CN105628882A (en) * | 2015-12-24 | 2016-06-01 | 孙莹莹 | On-site fast detection method for fruit and vegetable pesticide residues |
| CN107202610A (en) * | 2017-05-27 | 2017-09-26 | 湖北第二师范学院 | A kind of measurement techniques for quality detection of meat detection means |
-
2010
- 2010-01-07 CN CN201010022537XA patent/CN102121044A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| YI-HUA ZHENG ET AL: "Detection of dichlorvos residue by flow injection calorimetric biosensor based on immobilized chicken liver esterase", 《JOURNAL OF FOOD ENGINEERING》 * |
| 吴燕雯等: "提高鸡肝酯酶稳定性的保护剂选择", 《食品工业科技研究与探讨》 * |
| 徐斐等: "鸡肝酯酶在有机磷和氨基甲酸脂类农药检测中的应用研究", 《食品科学》 * |
| 毛斌等: "生物传感器在食品农药和抗生素残留检测中的应用", 《甘肃科学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103513012A (en) * | 2012-06-30 | 2014-01-15 | 句容鑫林农业科技服务有限公司 | Vegetable safety detection system and method |
| CN105628882A (en) * | 2015-12-24 | 2016-06-01 | 孙莹莹 | On-site fast detection method for fruit and vegetable pesticide residues |
| CN107202610A (en) * | 2017-05-27 | 2017-09-26 | 湖北第二师范学院 | A kind of measurement techniques for quality detection of meat detection means |
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Application publication date: 20110713 |