CN216051334U - Biochemical analysis and detection device for local surface plasma resonance - Google Patents
Biochemical analysis and detection device for local surface plasma resonance Download PDFInfo
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- CN216051334U CN216051334U CN202121683815.6U CN202121683815U CN216051334U CN 216051334 U CN216051334 U CN 216051334U CN 202121683815 U CN202121683815 U CN 202121683815U CN 216051334 U CN216051334 U CN 216051334U
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Abstract
The utility model discloses a local surface plasma resonance biochemical analysis and detection device. The device comprises a buffer liquid tank, an online degasser, a local surface plasma resonance optical system, a six-way valve and a sample injector. The utility model connects a set of on-line degasser in series at the entrance of the existing local surface plasma resonance optical system, eliminates the air bubble in the buffer solution of the local surface plasma resonance system, and the air bubble in the buffer solution enters the on-line degasser to be removed, thus eliminating the interference of the air bubble to the detection. The online degasser and the local surface plasma resonance optical system are connected in series through the six-way valve, and the mode can automatically complete system cleaning, reduce the possibility of sample pollution and save the sample processing time. The device is simple to operate, has obvious bubble eliminating effect, and greatly improves the accuracy of detection results, thereby ensuring the detection sensitivity of the system and facilitating the operation and maintenance of workers.
Description
Technical Field
The utility model relates to the technical field of photoelectric sensing detection, in particular to a local surface plasma resonance biochemical analysis and detection device.
Background
The Localized Surface Plasmon Resonance (LSPR) technology is a new generation SPR technology using gold nanoparticles as a detection unit. Different from the traditional SPR technology for detecting the deflection of an SPR angle caused by the change of the refractive index, LSPR detects the displacement of a light absorption peak generated by the change of the thickness of a molecular layer on the surface of a gold nanoparticle, and because the change of the light wavelength is little influenced by the environment and is insensitive to the interference of changes of volume, temperature, buffer solution refractive index and the like, the detection result of the series of products adopts a simpler light path system and an operation process while ensuring the precision and sensitivity similar to the traditional SPR technology, thereby greatly reducing the experimental threshold of the SPR technology and realizing a reproducible chip. Meanwhile, various costs of later use are reduced. The series of advantages enable the LSPR technology to be expected to become a necessary technical platform support for life science research experiments.
At present, in the use process of a local surface plasma resonance biochemical analysis and detection device, data obtained by analysis and detection are often inaccurate due to the fact that bubbles are dissolved in solution flowing into the system, so that the obtained analysis result has large error, and great trouble is caused to analysis and detection work.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to a localized surface plasmon resonance biochemical analysis and detection apparatus, which overcomes the above-mentioned shortcomings of the prior art.
In order to achieve the purpose, the utility model adopts the following technical scheme:
the utility model relates to a local surface plasma resonance biochemical analysis and detection device, which comprises a buffer liquid tank, an online degasser, a local surface plasma resonance optical system, a six-way valve and a sample injector, wherein the buffer liquid tank is connected with the online degasser;
the buffer tank with the inlet of online degasser is linked together, the liquid outlet of online degasser with the first valve of six-way valve is linked together, local surface plasmon resonance optical system's introduction port with the sixth valve of six-way valve is linked together, the sample injector includes quantitative ring, quantitative ring's both ends mouth respectively with the second valve and the fifth valve of six-way valve are linked together, the third valve and the appearance pipeline of advancing of six-way valve communicate mutually, the fourth valve and the first waste liquid jar intercommunication of six-way valve.
Furthermore, the buffer solution tank is communicated with a liquid inlet of the online degasser through a first pipeline, a filter is arranged in the buffer solution tank, one end of the first pipeline is communicated with a liquid outlet of the filter, and the other end of the first pipeline is detachably communicated with the liquid inlet of the online degasser.
Furthermore, the buffer liquid tank is arranged above the online degasser, the online degasser is arranged above the local surface plasmon resonance optical system, the first pipeline is provided with a liquid delivery pump, an inlet of the liquid delivery pump is communicated with the buffer liquid tank, and an outlet of the liquid delivery pump is communicated with the online degasser.
Further, online degasser includes working chamber, degasification pipe, pressure sensor, controller and vacuum pump, the degasification pipe sets up in the working chamber, the inlet end of vacuum pump with the working chamber intercommunication, pressure sensor is used for detecting the pressure of working chamber, pressure sensor with controller electric connection.
Furthermore, one end of the degassing pipe is connected with a liquid inlet of the online degasser, the other end of the degassing pipe is connected with a liquid outlet of the online degasser, and the liquid outlet of the online degasser is communicated with the first valve of the six-way valve through a second pipeline.
Further, the tube wall of the degassing tube is composed of a degassing film, and the degassing film is composed of a plurality of layers of central control fiber films.
Furthermore, a two-way valve is arranged on the second pipeline, one valve of the two-way valve is communicated with the first valve of the six-way valve, the other valve of the two-way valve is connected with a third pipeline, and the third pipeline is communicated with a second waste liquid tank.
Furthermore, a sample inlet of the local surface plasmon resonance optical system is communicated with a sixth valve of the six-way valve through a fourth pipeline, and a liquid outlet of the local surface plasmon resonance optical system is communicated with the second waste liquid tank.
Further, the sample injector still includes the sample bottle, the sample bottle passes through the appearance pipeline with the third valve intercommunication of six-way valve, be provided with the peristaltic pump on the appearance pipeline of advancing, the entry and the sample bottle intercommunication of peristaltic pump, the export of peristaltic pump with appearance pipeline intercommunication advances.
Furthermore, the first pipeline, the second pipeline, the third pipeline, the fourth pipeline and the sample injection pipeline are FEP pipes, silicone tubes or PEEK pipes.
The technical scheme provided by the utility model has the beneficial effects that:
(1) the utility model connects a set of on-line degasser in series at the entrance of the existing local surface plasma resonance optical system, and the air bubble in the buffer solution for washing the local surface plasma resonance optical system is removed after entering the on-line degasser, thus eliminating the interference of the air bubble to the detection. The online degasser and the local surface plasma resonance optical system are connected in series through the six-way valve, and by the mode, the system cleaning can be automatically completed, the possibility of sample pollution is reduced, and the sample processing time is saved.
(2) The device provided by the utility model is simple to operate, has an obvious bubble eliminating effect, and greatly improves the accuracy of a detection result, so that the detection sensitivity of the system is ensured, and the operation and maintenance of workers are facilitated.
Drawings
FIG. 1 is a schematic structural diagram of a localized surface plasmon resonance biochemical analysis and detection apparatus according to the present invention;
FIG. 2 is a schematic view of the structure of the on-line degasser of this embodiment.
1. A buffer liquid tank; 11. a first pipeline; 111. a filter; 112. an infusion pump; 2. an online degasser; 21. a working chamber; 22. a degassing tube; 221. a tube wall; 23. a pressure sensor; 24. a controller; 25. a vacuum pump; 26. a second pipeline; 261. a two-way valve; 262. a third pipeline; 3. a localized surface plasmon resonance optical system; 31. a fourth pipeline; 4. a six-way valve; 41. a first valve; 42. a second valve; 43. a third valve; 44. a fourth valve; 45. a fifth valve; 46. a sixth valve; 5. a sample injector; 51. a dosing ring; 52. a peristaltic pump; 521. a sample introduction pipeline; 53. a sample bottle; 6. a first waste liquid tank; 7. a second waste liquid tank.
Detailed Description
The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.
As shown in fig. 1, the local surface plasmon resonance biochemical analysis and detection device provided by the present invention comprises a buffer tank 1, an online degasser 2, a local surface plasmon resonance optical system 3, a six-way valve 4 and a sample injector 5; the buffer liquid tank 1 is communicated with a liquid inlet of the online degasser 2, a liquid outlet of the online degasser 2 is communicated with a first valve 41 of the six-way valve 4, a sample inlet of the local surface plasmon resonance optical system 3 is communicated with a sixth valve 46 of the six-way valve, the sample injector 5 comprises a quantitative ring 51, two ports of the quantitative ring 51 are respectively communicated with a second valve 42 and a fifth valve 45 of the six-way valve 4, a third valve 43 of the six-way valve 4 is communicated with a sample inlet pipeline 521, and a fourth valve 44 of the six-way valve 4 is communicated with the first waste liquid tank 6. The utility model connects a set of on-line degasser in series at the entrance of the existing local surface plasma resonance optical system, and the air bubble in the buffer solution for washing the local surface plasma resonance optical system is removed after entering the on-line degasser, thus eliminating the interference of the air bubble to the detection. In addition, the online degasser and the local surface plasma resonance optical system are connected in series through the six-way valve, and the system can be automatically cleaned in such a way, so that the possibility of sample pollution is reduced, and the sample processing time is saved. The device provided by the utility model is simple to operate, has an obvious bubble eliminating effect, and greatly improves the accuracy of a detection result, so that the detection sensitivity of the system is ensured, and the operation and maintenance of workers are facilitated.
The positions of the components are placed in various ways, which are not limited herein, in this embodiment, in order to achieve better de-bubbling effect and reduce de-bubbling time, the buffer tank 1 may be disposed above the online degasser 2, the online degasser 2 is disposed above the localized surface plasmon resonance optical system 3, the buffer tank 1 is communicated with the liquid inlet of the online degasser 2 through a first pipeline 11, a filter may be further disposed in the buffer tank 1, one end of the first pipeline 11 is communicated with the liquid outlet of the filter, and the other end of the first pipeline 11 is detachably communicated with the liquid inlet of the online degasser 2. The filter can filter out impurity particles in the buffer solution, so as to prevent the online degasser from being blocked and further ensure the removal of bubbles.
In order to control the liquid flow rate and ensure a stable pressure of the system, an infusion pump 112 may be further provided on the first tubing 11, an inlet of the infusion pump 112 being in communication with the buffer tank, and an outlet of the infusion pump 112 being in communication with the in-line degasser 2.
The structure of the in-line degasser 2 has various forms, which are not limited herein, and in the present embodiment, as shown in fig. 2, the structure of the in-line degasser 2 may include a working chamber 21, a degassing pipe 22, a pressure sensor 23, a controller 24 and a vacuum pump 25, the degassing pipe 22 may be disposed in the working chamber 21, an inlet end of the vacuum pump 25 is communicated with the working chamber 21, the pressure sensor 23 is used for detecting the pressure of the working chamber 21, and the pressure sensor 23 is electrically connected with the controller 24. Vacuum pump 25 is turned on by controller 24, vacuum pump 25 is operated to create a partial vacuum in chamber 21, the vacuum being measured by pressure transducer 23, and as solvent flows through degassing tube 22, dissolved gases in the solvent will permeate through the degassing membrane of degassing tube 22 and into chamber 21. In order to achieve complete removal of bubbles, the wall 221 of the degassing tube 22 may be composed of a degassing membrane in which a plurality of groups of central control fiber membranes are arranged. When the solvent flows into the outlet of the in-line degasser, the solvent is almost completely degassed and does not contain any gas.
In order to realize the series connection of the in-line degasser and the localized surface plasmon resonance system, one end of the degassing tube 22 is connected to the liquid inlet of the in-line degasser 2, the other end of the degassing tube is connected to the liquid outlet of the in-line degasser 2, and the liquid outlet of the in-line degasser 2 is connected to the first valve of the six-way valve 4 through a second line 26. The sample inlet of the local surface plasmon resonance optical system 3 is communicated with the sixth valve of the six-way valve 4 through a fourth pipeline 31, and the liquid outlet of the local surface plasmon resonance optical system 3 is communicated with the second waste liquid tank 7.
In order to effectively prevent air bubbles from entering the system, a two-way valve 261 may be further provided on the second pipeline 26, one valve of the two-way valve 261 is communicated with the first valve 41 of the six-way valve 4, the other valve of the two-way valve 261 is connected with a third pipeline 262, and the third pipeline 262 is communicated with the second waste liquid tank 7.
The sample injector 5 further comprises a sample bottle 53, the sample bottle 53 is communicated with the third valve 43 of the six-way valve 4 through a sample pipeline 521, in order to ensure the stability of the sample injection system of the device and improve the analysis and detection efficiency, a peristaltic pump 52 can be further arranged on the sample pipeline 521, the inlet of the peristaltic pump 52 is communicated with the sample bottle 53, and the outlet of the peristaltic pump 52 is communicated with the sample pipeline 521.
The pipeline material in this device requires for needing high pressure resistant, acidproof, alkali-proof and resistant organic reagent, in order to reduce the pollution and be convenient for observe, the pipeline material can be FEP pipe, silicone tube or PEEK pipe.
In order to make the workers more clearly understand the pipeline connection of the analysis and detection device provided by the utility model and facilitate the maintenance and the repair of the workers, the following detailed description is made according to different working states of the local surface plasmon resonance biochemical analysis and detection device provided by the utility model:
(1) when the analysis and detection device provided by the utility model carries out the defoaming work of the solvent in the buffer liquid tank 1, the infusion pump 112 is started, the solvent in the buffer liquid tank 1 flows through the first pipeline 11 through the filter 111, flows into the liquid inlet of the online degasser 2, then flows into the degassing pipe 22, carries out the defoaming, flows out from the liquid outlet of the online degasser 2, and the solvent continuously flows through the two-way valve 261, then flows into the third pipeline 262 and is finally discharged into the second waste liquid tank 7;
(2) when the analysis and detection device provided by the utility model carries out de-bubbling work of the detection device, the infusion pump 112 is started, the solvent in the buffer liquid tank 1 flows through the first pipeline 11 through the filter 111, flows into the liquid inlet of the online degasser 2, then flows into the degassing pipe 22, carries out de-bubbling, flows out of the liquid outlet of the online degasser 2, continuously flows through the two-way valve 261, flows into the first valve 41 of the six-way valve 4, flows into the sixth valve 46 through the first valve 41, flows into the liquid inlet of the local surface plasmon resonance optical system 3 through the fourth pipeline 31, washes the local surface plasmon resonance optical system 3, and then is discharged into the second waste liquid tank 7 from the liquid outlet of the local surface plasmon resonance optical system 3;
(3) when the analysis and detection device provided by the utility model performs sample analysis and detection, the peristaltic pump 52 is started, the sample solution sample introduction pipeline 521 in the sample bottle 53 flows through the third valve 43 of the six-way valve 4, flows into the second valve 42 through the third valve 43, flows into one port of the quantitative ring 51, the sample solution fills the quantitative ring 51, flows into the fifth valve 45 of the six-way valve 4 from the other port of the quantitative ring 51, flows into the sixth valve 46 through the fifth valve 45, flows into the liquid inlet of the localized surface plasmon resonance optical system 3 through the fourth pipeline 31, is detected in the localized surface plasmon resonance optical system, and is discharged into the second waste liquid tank 7 from the liquid outlet of the localized surface plasmon resonance optical system 3;
(4) when the analyzer provided by the present invention performs the sample injector flushing operation, the peristaltic pump 52 is activated, the sample solution injection line 521 in the sample bottle 53 flows through the third valve 43 of the six-way valve 4, flows into the second valve 42 through the third valve 43, flows into one port of the quantitative loop 51, fills the quantitative loop 51 with the sample solution, flows into the fifth valve 45 of the six-way valve 4 from the other port of the quantitative loop 51, flows into the fourth valve 44 through the fifth valve 45, and then is discharged into the first waste liquid tank 6.
The above is not relevant and is applicable to the prior art.
While certain specific embodiments of the present invention have been described in detail by way of illustration, it will be understood by those skilled in the art that the foregoing is illustrative only and is not limiting of the scope of the utility model, as various modifications or additions may be made to the specific embodiments described and substituted in a similar manner by those skilled in the art without departing from the scope of the utility model as defined in the appending claims. It should be understood by those skilled in the art that any modifications, equivalents, improvements and the like made to the above embodiments in accordance with the technical spirit of the present invention are included in the scope of the present invention.
Claims (10)
1. A local surface plasma resonance biochemical analysis and detection device is characterized in that: the device comprises a buffer liquid tank (1), an online degasser (2), a local surface plasma resonance optical system (3), a six-way valve (4) and a sample injector (5);
buffer tank (1) with the inlet of online degasser (2) is linked together, the liquid outlet of online degasser (2) with first valve (41) of six-way valve (4) are linked together, the introduction port of local area surface plasmon resonance optical system (3) with sixth valve (46) of six-way valve (4) are linked together, sample injector (5) are including ration ring (51), the both ends mouth of ration ring (51) respectively with second valve (42) and fifth valve (45) of six-way valve (4) are linked together, third valve (43) and introduction pipeline (521) of six-way valve (4) are linked together, fourth valve (44) and first waste liquid jar (6) intercommunication of six-way valve (4).
2. The localized surface plasmon resonance biochemical analysis detection apparatus of claim 1, wherein: the buffer liquid tank (1) is communicated with a liquid inlet of the online degasser (2) through a first pipeline (11), a filter is arranged in the buffer liquid tank (1), one end of the first pipeline (11) is communicated with a liquid outlet of the filter, and the other end of the first pipeline (11) is detachably communicated with the liquid inlet of the online degasser (2).
3. The apparatus for biochemical analysis by localized surface plasmon resonance as defined in claim 2, wherein: buffer solution jar (1) is arranged in online degasser (2) top, online degasser (2) are arranged in the top of local area surface plasmon resonance optical system (3), be provided with transfer pump (112) on first pipeline (11), the entry of transfer pump (112) with buffer solution jar (1) intercommunication, the export of transfer pump (112) with online degasser (2) intercommunication.
4. The apparatus for biochemical analysis by localized surface plasmon resonance as defined in claim 2, wherein: online degasser (2) include working chamber (21), degasification pipe (22), pressure sensor (23), controller (24) and vacuum pump (25), degasification pipe (22) set up in working chamber (21), the inlet end of vacuum pump (25) with working chamber (21) intercommunication, pressure sensor (23) are used for detecting the pressure of working chamber (21), pressure sensor (23) with controller (24) electric connection.
5. The apparatus according to claim 4, wherein the plasma resonance biochemical analysis and detection device comprises: one end of the degassing pipe (22) is connected with a liquid inlet of the online degasser (2), the other end of the degassing pipe is connected with a liquid outlet of the online degasser (2), and the liquid outlet of the online degasser (2) is communicated with a first valve of the six-way valve (4) through a second pipeline (26).
6. The localized surface plasmon resonance biochemical analysis detection apparatus of claim 5, wherein: the tube wall (221) of the degassing tube (22) is composed of a degassing film which is composed of a plurality of layers of central control fiber films.
7. The localized surface plasmon resonance biochemical analysis detection apparatus of claim 5, wherein: a two-way valve (261) is arranged on the second pipeline (26), one valve of the two-way valve (261) is communicated with the first valve (41) of the six-way valve (4), the other valve of the two-way valve (261) is connected with a third pipeline (262), and the third pipeline (262) is communicated with the second waste liquid tank (7).
8. The apparatus according to claim 7, wherein the apparatus comprises: and a sample inlet of the local surface plasma resonance optical system (3) is communicated with a sixth valve of the six-way valve (4) through a fourth pipeline (31), and a liquid outlet of the local surface plasma resonance optical system (3) is communicated with the second waste liquid tank (7).
9. The localized surface plasmon resonance biochemical analysis detection apparatus of claim 1, wherein: injector (5) still includes sample bottle (53), sample bottle (53) pass through advance kind pipeline (521) with third valve (43) intercommunication of six-way valve (4), be provided with peristaltic pump (52) on advance kind pipeline (521), the entry and sample bottle (53) intercommunication of peristaltic pump (52), the export of peristaltic pump (52) with advance kind pipeline (521) intercommunication.
10. The localized surface plasmon resonance biochemical analysis detection apparatus of claim 8, wherein: the first pipeline (11), the second pipeline (26), the third pipeline (262), the fourth pipeline (31) and the sample injection pipeline (521) are all FEP pipes, silicone tubes or PEEK pipes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121683815.6U CN216051334U (en) | 2021-07-22 | 2021-07-22 | Biochemical analysis and detection device for local surface plasma resonance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121683815.6U CN216051334U (en) | 2021-07-22 | 2021-07-22 | Biochemical analysis and detection device for local surface plasma resonance |
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| Publication Number | Publication Date |
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| CN216051334U true CN216051334U (en) | 2022-03-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121683815.6U Active CN216051334U (en) | 2021-07-22 | 2021-07-22 | Biochemical analysis and detection device for local surface plasma resonance |
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- 2021-07-22 CN CN202121683815.6U patent/CN216051334U/en active Active
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