CN2541463Y - Culture appts. for single cell Algae - Google Patents
Culture appts. for single cell Algae Download PDFInfo
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- CN2541463Y CN2541463Y CN 02214168 CN02214168U CN2541463Y CN 2541463 Y CN2541463 Y CN 2541463Y CN 02214168 CN02214168 CN 02214168 CN 02214168 U CN02214168 U CN 02214168U CN 2541463 Y CN2541463 Y CN 2541463Y
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- inlet pipe
- cavity
- unicellular algae
- culture apparatus
- algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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Abstract
The utility model provides a unicellular algae cultivating device, comprising a sealing cavity which is at least partially transparent, the upper part of the cavity is provided with an air vent, the cavity is connected with a water inlet pipe and an air inlet pipe, and generally the circumference of the cavity is equipped with manpower light source; the optimal scheme of the cavity is a soft film bag, and a rigid net is circled around the outer side of the film bag; and the air inlet pipe is connected with a disinfecting and a sterilizing devices of the air, and the water inlet pipe is connected with a filtering and the disinfecting devices of the liquid. Disinfecting seawater and nutrient salt are added into the cavity through the water inlet pipe; and the air or the mixed air which contains 1 to 10 % of CO2 is added into the cavity through the air inlet pipe. The unicellular algae cultivated in the device is not easy to pollute; the translucent degree is good and can guarantee enough light for the algae to grow; the utility model guarantees to provide enough CO2 required by the photosynthetic activity of the unicellular algae; and the accommodation area is small, the cultivation saves the time and the labor, the temperature condition required by the algae production is easy to control, and the quality of the unicellular algae is high.
Description
Described technical field
The utility model relates to a kind of biological culture device, is specifically related to the culture apparatus of a kind of unicellular algae.
Background technology
Unicellular algae such as chlorella, benthic diatom etc. are the bait of the young such as fishery products such as shrimp, scallop, sea cucumber, and along with the development of aquaculture, the unicellular algae demand is increasing.Unicellular algae is cultivated and generally adopts the open cultivation of cement pit at present, there are many shortcomings in this training mode, mainly show: 1, owing to adopt open cultivation, algae liquid and air contact surface are big, and many adrift harmful organisms in the air are easy to be splashed into addle, fall into foul, some insects are also invaded cultivation pool easily, all can bring harmful organisms come into, cause pollution.Because the harm of harmful organisms, this training method can only be done limited cultivation, can only cultivate about 15 days from being inoculated into fully results, even lower, just need to insert new algae kind, otherwise harmful organisms will breed in a large number, causes and cultivates failure.2, illumination is the important factor of algae photosynthesis, and is very big to cultivating the algae grows relation.At present used cement pit is cultivated, and can only penetrate light from the water surface.Light penetrates in the algae liquid because the absorption and the scattering of frustule, along with the increase intensity of illumination of the degree of depth reduces rapidly, only the cell of upper strata Chi Shuizhong can absorb and carry out photosynthetic saturated intensity of illumination, and the intensity of illumination that most of cell absorbs is insufficient, even the cell at the bottom of the pond is actual is among " dark ", has had a strong impact on the production breeding of unicellular algae.3, the growth and breeding of unicellular algae needs enough CO
2Carry out photosynthesis, the cement pit training method water surface is excessive, and modes such as hand mixing can not provide competent CO
2Under the situation of artificial culture unicellular algae, algae cell density is big, carries out the required CO of photosynthesis
2Supply is often not enough, becomes the limiting factor of micro algae growth, thereby has influenced the growth and breeding of unicellular algae.4, the cement pit floor space is big, and space availability ratio is little, the management trouble, and workload is big, and the necessary temperature condition of algae culture is difficult to control.The various shortcoming that the mode of above-mentioned cement pit cultivation algae exists, cause that the unicellular algae speed of growth of cultivating is slow, food quality is poor, culture density is little (at present, the chrysophyceae culture density is about 2,000,000/ml, little diatom is about 3,000,000/ml), harmful organisms is seriously polluted, can not produce for growing seedlings the bait of guaranteeing both quality and quantity is provided, seriously restrict the development of growing seedlings and producing.
Summary of the invention
The utility model has overcome above-mentioned cement pit and has cultivated unicellular algae easily by the murder by poisoning biological pollution; The illumination deficiency; The CO that algae photosynthesis is required
2Under-supply; Floor space is big, and space availability ratio is little, and workload is big, and the unmanageable shortcoming of temperature condition that algae culture is necessary provides a kind of novel unicellular algae culture apparatus, and the unicellular algae that this device is cultivated is difficult for contaminated; Good transmittance can guarantee the enough light of algal grown; Assurance provides algae photosynthesis required enough CO
2Floor space is little, and it is time saving and energy saving to cultivate, and algae produces essential temperature condition and is easy to control, the algae quality height of cultivation.
The utility model discloses the culture apparatus of a kind of unicellular algae.This device comprises the enclosed housing of partially transparent at least, and cavity top sets out pore, and described cavity links to each other with water inlet pipe, inlet pipe.The culturing process of unicellular algae is in the utility model: the cavity that unicellular algae is placed partially transparent, in the cavity splendid attire after filtration with the disinfectant nutrient solution in, nutrient solution is generally after filtration, the disinfectant seawater, charges into the 1-10%CO that contains through sterilization and sterilization in nutrient solution continuously or regularly
2And nutritive salt.Unicellular algae is growth and breeding in illumination and competent gnotobasis.Because the cultivation of unicellular algae needs bubbling air or contains 1-10%CO
2Mixing air, therefore production well need be left in the top of cavity, with with unnecessary air scavenge cavity, described production well can be provided with separately, also can work as inlet pipe and/or water inlet pipe when being arranged on the top of cavity, and inlet pipe and/or water inlet pipe are connected with the cavity non-tight, sterilization seawater and nutritive salt add from water inlet pipe or air outlet, and when water inlet pipe and inlet pipe when the cavity bottom is connected with cavity, leak outside in cavity for preventing nutritive medium, inlet pipe and water inlet pipe need be tightly connected with cavity.
Source of artificial light is set around the cavity of common above-mentioned partially transparent.Because growth and the breeding of unicellular algae need light source, for bonding cell algal grown and breeding have competent light source, also can grow and breed the indoor or night a little less than light, at culture apparatus source of artificial light is set on every side.
Usually cavity is set to the software bag film, wound film bag arranged outside rigidity rack.In the utility model, all can be used for process chamber as long as can guarantee the cavity material transparent, as glass, transparent plastics etc.Because the polythene film bag cost is low, good transmittance is so preferred version is selected the bag film by PE Processing for use, because the polyethylene film bag is soft, so at the arranged outside rigid support of wound film bag cavity.
Described adding air or contain 1-10%CO
2The inlet pipe of mixing air link to each other with sterilization, the sterilising plant of gas, add the air in the cavity or contain 1-10%CO
2Mixing air behind sterilization, add by inlet pipe; Described water inlet pipe links to each other with liquid filtering, sterilizing unit.Add in the cavity nutrient solution after filtration, disinfect the back and add by water inlet pipe.On liquid filtering, the sterilizing unit market multiple model is arranged, as the NLC-2000 and the NLC-5000 uv steriliser of the New World, Fujian company production.And in the sterilization of gas, sterilising plant known technology multiple device is arranged.
Usually the air outlet of inlet pipe places the bottom or the bottom of cavity, to guarantee air or to contain 1-10%CO
2Mixing air many to the greatest extent dissolve in nutrient solution, help the growth of unicellular algae; Gas stone is the close porous stone material of a germplasm, and the air outlet can expose setting, also can connect gas stone in the air outlet, connects gas stone and can make the increase air or contain 1-10%CO
2Mixing air and the contact area of nutrient solution, increase CO
2Meltage is beneficial to growth and the breeding of unicellular algae.
The cavity bottom connects collection tube, is convenient to collect unicellular algae.
By regulating CO in the gas that adds
2Content, can be to a certain extent provide optimum CO in the nutritive medium for unicellular algae growth and breeding
2Concentration, thus on the inlet pipe in the outside that the sterilization of gas described in the utility model, sterilising plant link to each other with described cavity gas mixer is set, to regulate the CO in the gas that adds in the cavity
2Content.
Owing to adopt the culture apparatus of the transparent cavity of sealing as unicellular algae, good airproof performance reduces even has cut off and the contacting of the external world, and has reduced the harm of harmful organisms, can carry out long-term cultivation in the utility model.When unicellular algae density reaches the results degree, results parts, another part adds cultivations that continue of new nutrient solution, and cultivation capable of circulation like this is more than 2 months, and has the biological appearance of harmful animal; This culture apparatus light transmission is good, and all frustules can both absorb and carry out the required saturated intensity of illumination of photosynthesis, have accelerated the growth and breeding speed of unicellular algae; By adding the disinfectant air in the nutrient solution of inlet pipe in cavity or containing 1-10%CO
2Mixing air, guaranteed CO
2Supply fully helps the growth and breeding of unicellular algae; This device cultivation unicellular algae floor space is little, cultivates simply, and is time saving and energy saving, can place suitable location with cultivating cavity as required simultaneously, regulates culture temperature as required.By culture apparatus of the present utility model, the fast growth of unicellular algae, culture density is big, and is with short production cycle.Because the density of unicellular algae is big, reduced nutritive salt in the bait water body to cultivating the pollution of water body, food quality is good, and the algae of cultivating with this culture apparatus is difficult for aging, has reduced the harm of wearing out and causing growing seedlings because of bait.
Description of drawings
Fig. 1 is the stereographic map of the unicellular algae culture apparatus of tool embodiment 1.
Fig. 2 is the stereographic map of the unicellular algae culture apparatus of tool embodiment 2.
Fig. 3 is the synoptic diagram of embodiment 3.
Embodiment
Embodiment 1
Culture apparatus as unicellular algae that Fig. 1 looks, comprise software sealed transparent plastics bag 1, around the arranged outside rigidity rack 2 of plastics bag 1, the top of plastics bag 1 is provided with two openings, water inlet pipe 3 passes opening one end and places plastics bag 1, air or contain 1-10%CO
2The inlet pipe 4 of mixing air pass another opening one end and be arranged in the plastics bag 1, inlet pipe 4 is connected with cavity 1 non-tight with water inlet pipe 3, so that unnecessary gas is discharged in cavity; The air outlet of inlet pipe 4 is arranged in the bottom of cavity, and the place, air outlet connects gas stone 9.Inlet pipe 4 links to each other 6 with gas depoisoning, sterilising plant, and water inlet pipe 3 links to each other with liquid filtering, sterilizing unit 5.Plastics bag 1 bottom connects collection tube 7.
As the culture apparatus of unicellular algae that Fig. 2 looks, comprise software sealed transparent plastics bag 1, around the arranged outside rigidity rack 2 of plastics bag 1, the top of plastics bag 1 is provided with an opening, and water inlet pipe 3 is with air or contain 1-10%CO
2The inlet pipe 4 of mixing air pass described opening one end and be arranged in the plastics bag 1, the opening end of inlet pipe 4 is exposed, inlet pipe 4 is connected with the upper opening non-tight of water inlet pipe 3 with cavity 1, so that unnecessary gas is discharged in cavity; The air outlet of inlet pipe 4 is arranged in the bottom of cavity.Inlet pipe 4 links to each other with gas depoisoning, sterilising plant 6, on the inlet pipe in the outside that the sterilization of described gas, sterilising plant 6 link to each other with described cavity gas mixer 8 is set; Water inlet pipe 3 links to each other with liquid filtering, sterilizing unit 5.Plastics bag 1 bottom connects collection tube 7.Source of artificial light 10 is set around the cavity 1.
In the utility model, culture apparatus that can unicellular algae is set to the culture apparatus looked as Fig. 3, being about to a plurality of transparent plastics bag 1 couples together by pipeline, and only use a liquid filtering, sterilizing unit 5 and gaseous sterilization, sterilizing unit 6, so setting can be carried out large batch of unicellular algae cultivation, with time saving and energy saving.
The utility model can link to each other inlet pipe and/or water inlet pipe with the lower seal of cavity, the outlet orifice of gas is arranged on the top of cavity to discharge unnecessary gas, can realize the purpose of this utility model and effect equally, in the utility model because air or contain 1-10%CO
2Mixing air and nutritive medium can not add simultaneously, so can inlet pipe and water inlet pipe be set to same pipeline.Above-mentioned design belongs to protection domain of the present utility model equally.
Claims (10)
1, the culture apparatus of a kind of unicellular algae is characterized in that: this device comprises the enclosed housing of partially transparent at least, and cavity top sets out pore, and described cavity links to each other with water inlet pipe, inlet pipe.
2, the culture apparatus of unicellular algae according to claim 1 is characterized in that: source of artificial light is set around the described cavity.
3, the culture apparatus of unicellular algae according to claim 1 and 2 is characterized in that: described cavity is the software bag film, wound film bag arranged outside rigidity rack.
4, the culture apparatus of unicellular algae according to claim 1 and 2 is characterized in that: described inlet pipe links to each other with sterilization, the sterilising plant of gas, and described water inlet pipe links to each other with liquid filtering, sterilizing unit.
5, the culture apparatus of unicellular algae according to claim 3 is characterized in that: described inlet pipe links to each other with sterilization, the sterilising plant of gas, and described water inlet pipe links to each other with liquid filtering, sterilizing unit.
6, the culture apparatus of unicellular algae according to claim 1 and 2 is characterized in that: the air outlet of described inlet pipe is arranged in the bottom of cavity, the exposed or connection gas stone in air outlet.
7, the culture apparatus of unicellular algae according to claim 3 is characterized in that: the air outlet of described inlet pipe is arranged in the bottom of cavity, the exposed or connection gas stone in air outlet.
8, the culture apparatus of unicellular algae according to claim 1 and 2 is characterized in that: the cavity bottom connects collection tube.
9, the culture apparatus of unicellular algae according to claim 3 is characterized in that: the cavity bottom connects collection tube.
10, the culture apparatus of unicellular algae according to claim 4 is characterized in that: on the inlet pipe in the outside that the sterilization of described gas, sterilising plant link to each other with described cavity gas mixer is set.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02214168 CN2541463Y (en) | 2002-04-22 | 2002-04-22 | Culture appts. for single cell Algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02214168 CN2541463Y (en) | 2002-04-22 | 2002-04-22 | Culture appts. for single cell Algae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2541463Y true CN2541463Y (en) | 2003-03-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02214168 Expired - Fee Related CN2541463Y (en) | 2002-04-22 | 2002-04-22 | Culture appts. for single cell Algae |
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| CN (1) | CN2541463Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100375783C (en) * | 2006-03-21 | 2008-03-19 | 中盐制盐工程技术研究院 | Method for culturing 'Yuanshizao' alga in large scale outdoor, and its processing method |
| CN102268375A (en) * | 2011-06-03 | 2011-12-07 | 中国海洋大学 | Method and system for continuously collecting algae liquid cultured from unicellular algae |
| CN102408981A (en) * | 2010-09-26 | 2012-04-11 | 新奥科技发展有限公司 | Photo-bioreactor |
| CN104783155A (en) * | 2014-12-30 | 2015-07-22 | 甘肃德福生物科技有限公司 | A vehicle-mounted rescue nutrient solution supplying system |
| FR3101639A1 (en) * | 2019-10-04 | 2021-04-09 | La Belle Ondulée | DEVICE FOR THE PRODUCTION OF BIOMASS FROM CELL CULTURE OF MICROALGAE OR CYANOBACTERIA. |
| CN115404168A (en) * | 2022-11-01 | 2022-11-29 | 山东滨州八匹马塑料化纤制品有限公司 | Marine microalgae cultivation method |
-
2002
- 2002-04-22 CN CN 02214168 patent/CN2541463Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100375783C (en) * | 2006-03-21 | 2008-03-19 | 中盐制盐工程技术研究院 | Method for culturing 'Yuanshizao' alga in large scale outdoor, and its processing method |
| CN102408981A (en) * | 2010-09-26 | 2012-04-11 | 新奥科技发展有限公司 | Photo-bioreactor |
| CN102408981B (en) * | 2010-09-26 | 2014-09-17 | 新奥科技发展有限公司 | Photo-bioreactor |
| CN102268375A (en) * | 2011-06-03 | 2011-12-07 | 中国海洋大学 | Method and system for continuously collecting algae liquid cultured from unicellular algae |
| CN102268375B (en) * | 2011-06-03 | 2012-10-03 | 中国海洋大学 | Method and system for continuously collecting algae liquid cultured from unicellular algae |
| CN104783155A (en) * | 2014-12-30 | 2015-07-22 | 甘肃德福生物科技有限公司 | A vehicle-mounted rescue nutrient solution supplying system |
| FR3101639A1 (en) * | 2019-10-04 | 2021-04-09 | La Belle Ondulée | DEVICE FOR THE PRODUCTION OF BIOMASS FROM CELL CULTURE OF MICROALGAE OR CYANOBACTERIA. |
| CN115404168A (en) * | 2022-11-01 | 2022-11-29 | 山东滨州八匹马塑料化纤制品有限公司 | Marine microalgae cultivation method |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |