CN221740304U - An intelligent cell preparation workstation - Google Patents

Abstract

The utility model discloses an intelligent cell preparation workstation which comprises a cell centrifugal processing station, a grabbing and transferring mechanism, a cell culture workstation, an empty bottle stock and a cell resuscitating mechanism, wherein the grabbing and transferring mechanism can receive a cell liquid plate frame assembly, the cell resuscitating mechanism can receive the cell liquid plate frame assembly on the grabbing and transferring mechanism and resuscitate cells in a cryopreservation tube in the cell liquid plate frame assembly, and the cell centrifugal processing station can receive the resuscitated cell liquid plate frame assembly and extract cell liquid in the cryopreservation tube on the cell liquid plate frame assembly for pipetting and centrifuging; and the cell centrifugal processing station can receive the feeding bottle for supplementary feeding, and the cell culture workstation can receive the cell liquid of the cell centrifugal processing station for cell culture. The utility model has the beneficial effects that the device completely realizes automation, does not need manual operation, saves a great amount of manual operation time, and avoids the defect of inaccurate operation of personnel, thereby improving the quality and efficiency of cell culture.

Description

An intelligent cell preparation workstation

Technical Field

The utility model relates to the technical field of biological cells, in particular to an intelligent cell preparation workstation.

Background Art

With the development of science and technology, bioengineering has higher standards for cell culture. Meeting the high consistency and large-scale cultivation of cells has become a trend of future development.

Traditional cell culture equipment has a low degree of automation. For example, operations such as loading and placing cell culture bottles, using cell culture incubators for cell culture, and labeling cell culture bottles need to be completed manually, and the corresponding work equipment is distributed in different locations, resulting in low work efficiency and high labor intensity for staff. In cell culture work, it is necessary to add a variety of culture media to the cell culture bottles, and different culture media need to be used in different culture processes. Therefore, there are strict requirements for operators and operating procedures. Operators should be familiar with the amount of culture media required in each process. Due to the different experience and techniques of operators, it is very easy to cause cell culture work to obtain very different results, which seriously affects cell culture. quality and efficiency; the entire cell culture process requires the use of multiple devices, which requires a large floor space, and there is a lack of automated connection between the multiple devices, which requires operators to spend a lot of time and effort, and cannot meet the needs of more efficient cell culture; and the current incubator can store fewer cell culture bottles, and it is necessary to manually observe the cell growth status of the culture bottles inside, which is time-consuming and laborious, and there is a possibility of misjudgment. It is necessary to manually place the required various bottles in fixed positions in advance, and load them regularly, which is cumbersome and easy to forget. After the cells are made, they need to be put into the box at a specific location, which is extremely inconvenient to use. Therefore, there is an urgent need for an intelligent cell preparation workstation.

Utility Model Content

The purpose of this section is to summarize some aspects of the embodiments of the utility model and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and invention title of this application to avoid blurring the purpose of this section, specification abstract and invention title, and such simplifications or omissions cannot be used to limit the scope of the utility model.

In view of the above problems or problems existing in the prior art, the present utility model is proposed.

Therefore, the purpose of the utility model is to provide an intelligent cell preparation workstation, which can automatically realize cell production without manual liquid addition, thereby ensuring the accuracy of liquid addition and greatly improving work efficiency.

In order to solve the above technical problems, the utility model provides the following technical solutions: an intelligent cell preparation workstation, which includes a cell centrifugation processing station, a grabbing and transporting mechanism, a cell culture workstation, an empty bottle material warehouse, and a cell recovery mechanism;

A cell centrifugation processing station and a cell recovery mechanism are arranged on one side of the grabbing and transporting mechanism, and a cell culture workstation and an empty bottle material warehouse are arranged on the other side;

The grabbing and transporting mechanism can receive the cell liquid plate rack assembly, the cell resuscitation mechanism can receive the cell liquid plate rack assembly on the grabbing and transporting mechanism, and perform cell resuscitation on the cryopreservation tubes in the cell liquid plate rack assembly, the cell centrifugal processing station can receive the resuscitated cell liquid plate rack assembly, and extract the cell liquid in the cryopreservation tubes on the cell liquid plate rack assembly for pipetting and centrifugation operations; and the cell centrifugal processing station can receive the feed bottle for supplementary feeding;

The cell culture workstation can receive cell fluid from the cell centrifugation processing station for cell culture;

The empty bottle material warehouse at least stores empty cell tubes, empty cell reaction bottles and cryopreservation tubes; the empty bottle material warehouse can provide empty cell tubes, empty cell reaction bottles and cryopreservation tubes for the cell centrifugation processing station and/or the cell culture workstation through a grabbing and transporting mechanism;

The cell centrifugation processing station can receive empty cell tubes and empty cell reaction bottles grabbed from the empty bottle storage by the grabbing and transporting mechanism.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the cell centrifugal processing station comprises an upper operating chamber and a centrifugal mechanism;

A centrifugal mechanism is provided at the lower end of the upper operating chamber;

The upper operating chamber is provided with a suction mechanism, a clamping mechanism, and a bottle support platform;

The bottle support table can be used to place cell tubes, cell reaction bottles and supply bottles;

The suction mechanism can suck the cell liquid in the cell liquid plate rack assembly and the culture liquid in the feed bottle into the cell tube, and the clamping mechanism can clamp the cell tube and put it into the centrifugal mechanism for centrifugation;

The suction mechanism can suck the cell liquid in the cell liquid plate rack assembly and the culture liquid in the feed bottle into the cell reaction bottle, and the grabbing and transporting mechanism can grab the cell reaction bottle and put it into the cell culture workstation for cell culture.

As a preferred solution of the intelligent cell preparation workstation of the utility model, a feeding mechanism is arranged below the upper operation chamber, and the feeding mechanism can supplement and feed the feeding bottles on the bottle support table.

As a preferred solution of the intelligent cell preparation workstation of the utility model, a first manipulator is arranged in the upper operation chamber, and the first manipulator can grab the feeding bottle on the feeding mechanism and place it on the feeding bottle support table.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the bottle support platform includes a cell tube support platform, a cell reaction bottle support platform, and a supply bottle support platform;

At least one group of cell tubes is stored on the cell tube support table, at least one group of cell reaction bottles is stored on the cell reaction bottle support table, and at least one group of supply bottles is stored on the supply bottle support table.

As a preferred solution of the utility model intelligent cell preparation workstation, the upper operating chamber also includes a cryotube rotating opening assembly; the cryotube rotating opening assembly can open the tube caps of the cryotubes on the cell liquid plate rack assembly in the upper operating chamber.

As a preferred solution of the intelligent cell preparation workstation of the utility model, it also includes a cell plate rack in-and-out storage component, which can receive an external cell liquid plate rack component.

As a preferred solution of the utility model intelligent cell preparation workstation, it also includes an identification component, which can scan and identify the cell liquid plate rack component.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the cell tube support platform includes a cell tube support plate driving member, a cell tube support plate, an upper limit tube slot, a lower limit tube rack, and a limiting rotating member;

The cell tube support disk driving member is connected to the cell tube support disk. The cell tube support disk is provided with at least one set of upper limit tube grooves and lower limit tube racks. The cell tubes can be placed in the upper limit tube grooves and the lower limit tube racks. The limiting rotation member can fix the lower end of the cell tube placed in the lower limit tube rack.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the cell reaction bottle support platform includes a cell reaction bottle turntable drive, a cell reaction bottle turntable, and a cell reaction bottle notch;

The cell reaction bottle turntable driving member is connected to the cell reaction bottle turntable. At least one group of cell reaction bottle notches is arranged on the cell reaction bottle turntable. Cell reaction bottles can be stored in the cell reaction bottle notches. The cell reaction bottle turntable driving member can drive the cell reaction bottle turntable to rotate.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the feed bottle support platform includes a feed bottle support plate, a feed bottle support plate driving member, and a storage bottle limiting hole;

The feeding bottle supporting plate driving member drives the feeding bottle supporting plate to rotate. At least one group of storage bottle limiting holes is arranged on the feeding bottle supporting plate, and the feeding bottles can be placed in the storage bottle limiting holes.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the cell liquid plate rack assembly includes a cryotube box support disk drive, a cryotube box support disk, a cryotube box support disk rotating shaft, and a cryotube box;

The cryotube box support disk driving member drives the cryotube box support disk to rotate via the cryotube box support disk rotating shaft. At least one set of sample box slots is arranged on the cryotube box support disk. Cryotube boxes can be stored in the sample box slots, and cryotubes can be stored in the cryotube boxes.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the upper operating chamber also includes an upper connecting frame; an upper connecting frame is arranged at the upper end of the upper operating chamber, an upper sliding guide rail is arranged on the upper connecting frame, and the suction mechanism and the clamping mechanism can move along the upper sliding guide rail.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the suction mechanism includes a first upper horizontal sliding guide member, a first upper vertical guide member, a second slider, a suction head driving mechanism, a suction head connecting groove, and a suction head;

The first upper horizontal sliding guide is slidably connected to the upper sliding guide, the first upper vertical guide is slidably connected to the first upper horizontal sliding guide, the second slider is vertically slidably connected to the first upper vertical guide, the second slider is connected to the suction head driving mechanism, and the suction head connecting groove is connected to the suction head.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the clamping mechanism includes a second upper horizontal sliding guide rail, a second upper vertical sliding guide rail, a first driving member, a first slider, a third upper vertical sliding guide rail, a second driving member, a rotating clamping portion, and a first connecting plate;

The second upper horizontal sliding guide rail is slidably connected to the upper sliding guide rail, the second upper vertical sliding guide rail is slidably connected to the second upper horizontal sliding guide rail, the second upper vertical sliding guide rail is provided with a first driving member, the first driving member can drive the first slider to slide vertically along the second upper vertical sliding guide rail, the third upper vertical sliding guide rail is connected to the first slider, the first connecting plate is vertically slidably connected to the second upper vertical sliding guide rail, the first connecting plate is provided with a second driving member, and the second driving member is connected to the rotating clamping portion.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the upper operating chamber also includes a first quick-change head assembly, which can be used by the first manipulator to replace different clamps, and the first quick-change head assembly includes at least one group of clamps.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the cryotube rotary opening assembly includes a vertical support seat, a first drive lifting mechanism, a second drive lifting mechanism, a turret opening member, a lifting fixing plate, a sensor; and a turret through hole;

A first driving lifting mechanism and a second driving lifting mechanism are arranged on the vertical support seat. The first driving lifting mechanism can drive the rotary head opening member to lift and lower, and the second driving lifting mechanism can drive the lifting fixing plate to lift and lower. The sensor is connected to the lifting fixing plate, and a rotary head through hole is opened on the lifting fixing plate.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the rotor opening member includes a side connection support plate, a middle connection support plate, a first rotating wheel, a second rotating wheel, a rotating belt, a transmission gear, a rotating shaft, a spring, a connecting tooth, and a cover opening rotor;

Side connecting support plates are connected to both sides of the middle connecting support plate, a top plate is arranged above the middle connecting support plate, the first rotating wheel is connected to the second rotating wheel through a rotating belt, the inner side of the rotating belt is meshed and connected with the transmission gear, the transmission gear is connected to the rotating shaft, the spring is arranged on the outer side of the rotating shaft, and the lower end of the rotating shaft is connected to the cover opening rotating head through connecting teeth.

As a preferred solution of the utility model intelligent cell preparation workstation, wherein: the centrifugal mechanism includes a centrifuge and a centrifuge support frame; the centrifuge support frame supports the centrifuge;

At least one group of centrifugal slots is arranged in the centrifuge, and cell tubes can be placed in the centrifugal slots. The centrifuge can centrifuge the cell tubes.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the upper operation chamber also includes a cell tube cell collection component, which can collect cells after centrifugation of the cell tube.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the feeding mechanism includes a feeding movable base, a feeding driving member, a moving track, a feeding movable plate, a feeding turntable driving member, a feeding turntable, and a storage bottle limiting hole;

A loading driving member is arranged on the loading movable base, and a moving track is arranged on the upper end of the loading driving member. The loading driving member can drive the loading movable plate to slide along the moving track. The upper end of the loading movable plate is connected with the loading turntable driving member, and the loading turntable driving member can drive the loading turntable to rotate. At least one group of storage bottle limiting holes is arranged on the loading turntable, and supply bottles are placed in the storage bottle limiting holes.

As a preferred solution of the intelligent cell preparation workstation of the utility model, a first laminar flow hood is arranged above the upper operation chamber, and a second laminar flow hood is arranged above the first laminar flow hood, the cell culture workstation, and the empty bottle material warehouse.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the upper operating chamber also includes a tip assembly and a counterweight assembly; a counterweight assembly is arranged on the side of the tip assembly, the tip assembly includes at least one group of tips, and the counterweight assembly is provided with at least one group of counterweights.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the upper operation chamber also includes a waste liquid and waste material collection mechanism, which collects waste water or waste material.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the waste liquid and waste material collection mechanism comprises a first waste liquid collection port, a second waste liquid collection port, a waste material docking port, a waste liquid storage tank, and a waste material storage tank;

The first waste liquid collection port, the second waste liquid collection port and the waste docking port are all arranged in the upper operating chamber, the waste liquid storage tank and the waste storage tank are arranged below the upper operating chamber, the first waste liquid collection port and the second waste liquid collection port are discharged into the waste liquid storage tank through a pipeline, and the waste docking port is discharged into the waste storage tank through a pipeline.

As a preferred solution of the intelligent cell preparation workstation of the utility model, a front flap door mechanism and a rear sealing door mechanism are respectively arranged on the two side walls of the upper operation chamber.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the grabbing and transporting mechanism includes a second manipulator and a second manipulator track;

The bottom end of the second robot is slidably connected to the second robot track.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: a second quick-change head assembly is arranged at the side end of the second robot rail, and at least one group of quick-change heads is arranged on the second quick-change head assembly.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the cell recovery mechanism includes a lifting and pressing component and a recovery sample slot;

The lifting and pressing component can immerse the cell liquid plate rack component in the recovery sample tank.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the lifting and lowering pressing assembly includes a pressing support, a first horizontal driving member, a second vertical driving member, a sample box support limit plate, a sample box pressing plate, and a pressing plate driving member;

A first horizontal driving member is arranged at the upper end of the pressing support member, the first horizontal driving member is connected to the second vertical driving member, the second vertical driving member is connected to the sample box support limiting plate, a pressing plate driving member is arranged on the sample box support limiting plate, and the pressing plate driving member drives the sample box pressing plate to rise and fall.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: a heating element, a temperature measuring element, and an inlet and outlet liquid pipe are arranged in the resuscitation sample tank;

The inlet and outlet liquid pipes can inject or discharge liquid into the resuscitation sample tank, the heating element can heat the liquid, and the temperature measuring element can measure the temperature of the liquid.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the cell culture workstation includes a carbon dioxide incubator and an incubator door opening and closing mechanism;

An incubator door opening and closing mechanism is arranged on the side of the carbon dioxide incubator. The carbon dioxide incubator can store at least one group of cell reaction bottles. The fetching and transporting mechanism can grab the cell reaction bottles inside the carbon dioxide incubator through the incubator door opening and closing mechanism.

As a preferred solution of the intelligent cell preparation workstation of the utility model, at least one group of circular turntables is arranged in the carbon dioxide incubator, and a monitoring and lifting mechanism is arranged on the side of the circular turntable, which can monitor the internal state of the cell reaction bottle and grab it; at least one group of cell reaction bottles can be stored on the circular turntable.

As a preferred solution of the intelligent cell preparation workstation of the utility model, a rotating shaft is arranged in the carbon dioxide incubator, and multiple groups of circular turntables are vertically arranged around the rotating shaft.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: multiple groups of circular turntables can rotate independently or simultaneously.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the carbon dioxide incubator also includes a turntable drive mechanism; a turntable drive mechanism is arranged on the inner wall of the carbon dioxide incubator, and the turntable drive mechanism can drive the circular turntable to rotate around the rotation axis.

As a preferred solution of the intelligent cell preparation workstation of the utility model, each set of circular turntables is correspondingly provided with a turntable driving mechanism.

As a preferred solution of the intelligent cell preparation workstation of the utility model, a cell reaction bottle slot is provided on the circular turntable, side limit blocks are provided on both sides of the cell reaction bottle slot, and a top limit block is provided in front of the side limit block at one end close to the rotating shaft.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the monitoring and lifting mechanism includes a visual observation mechanism, a clamping mechanism, and a clamping drive member;

The clamping drive can drive the visual observation mechanism and the clamping mechanism to rise and fall, the clamping mechanism can support the visual observation mechanism, and the clamping mechanism can grab the cell reaction bottle on the circular turntable.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the empty bottle material warehouse includes at least one group of supporting material plates, on which cell reaction bottles, cell tubes and cryopreservation tubes can be placed.

As a preferred solution of the intelligent cell preparation workstation of the utility model, multiple groups of support material plates can be arranged vertically.

As a preferred solution of the intelligent cell preparation workstation of the utility model, the supporting material plate can be rotated or fixed.

As a preferred solution of the intelligent cell preparation workstation of the utility model, wherein: the supporting material plate includes a first supporting plate and a second supporting plate;

A second support plate is arranged on the upper end of the first support plate. Empty cell reaction bottles can be placed on the first support plate, and empty cryopreservation tubes and empty cell tubes can be placed on the second support plate.

As a preferred solution of the utility model intelligent cell preparation workstation, wherein: the grabbing and transporting mechanism can grab the target empty bottle on the supporting material plate and place it into the upper operating chamber.

As a preferred solution of the utility model intelligent cell preparation workstation, it also includes a transfer tank conveying mechanism, which is arranged on the side of the empty bottle material warehouse, the transfer tank conveying mechanism can receive external transfer tanks, and the grabbing and transporting mechanism can open the tank cover of the transfer tank.

The beneficial effects of the utility model are as follows: the utility model can receive the cell liquid plate rack assembly through the grabbing and transporting mechanism, and put it into the cell resuscitation mechanism for resuscitation; the resuscitated cell liquid plate rack assembly can be put into the cell centrifuge processing station, and the cell centrifuge processing station can extract the cells in the cell liquid plate rack assembly for pipetting and centrifugation operations; the cell culture workstation can receive the cell liquid from the cell centrifuge processing station for cell culture; the empty bottle material warehouse can provide empty cell tubes, empty cell reaction bottles and cryopreservation tubes for the cell centrifuge processing station and/or the cell culture workstation through the grabbing and transporting mechanism. The device is fully automated and does not require manual operation, thus saving a lot of manual operation time and avoiding the disadvantages of inaccurate human operation, thereby improving the quality and efficiency of cell culture.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following briefly introduces the drawings required for the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

Figure 1 is an overall schematic diagram of the intelligent cell preparation workstation.

FIG2 is a schematic diagram of the interior of the intelligent cell preparation workstation.

FIG. 3 is an internal top view of the intelligent cell preparation workstation.

FIG4 is a schematic diagram of the grabbing and transporting mechanism of the intelligent cell preparation workstation.

FIG. 5 is a schematic diagram of the second quick-change head assembly of the intelligent cell preparation workstation.

FIG. 6 is a schematic diagram of the transfer tank transmission mechanism of the intelligent cell preparation workstation.

FIG. 7 is a schematic diagram of the cell plate rack storage and retrieval components of the intelligent cell preparation workstation.

FIG8 is a schematic diagram of the empty bottle material warehouse of the intelligent cell preparation workstation.

FIG. 9 is a schematic diagram of a cell recovery mechanism of an intelligent cell preparation workstation.

FIG. 10 is a schematic diagram of a cell recovery mechanism of an intelligent cell preparation workstation.

FIG. 11 is a partial schematic diagram of the cell recovery mechanism of the intelligent cell preparation workstation.

FIG. 12 is a schematic diagram of a cell centrifugation processing station of an intelligent cell preparation workstation.

FIG. 13 is a schematic diagram of the interior of the cell centrifugation processing station of the intelligent cell preparation workstation.

FIG. 14 is a schematic diagram of the interior of the cell centrifugation processing station of the intelligent cell preparation workstation.

FIG. 15 is a partial schematic diagram of the interior of the cell centrifugation processing station of the intelligent cell preparation workstation.

FIG. 16 is a schematic diagram of the clamping mechanism of the intelligent cell preparation workstation.

FIG. 17 is a schematic diagram of the suction mechanism of the intelligent cell preparation workstation.

FIG. 18 is a schematic diagram of a cell liquid plate rack assembly of an intelligent cell preparation workstation.

FIG. 19 is a schematic diagram of a cell tube support platform of an intelligent cell preparation workstation.

FIG. 20 is a schematic diagram of a feed bottle support platform of an intelligent cell preparation workstation.

FIG. 21 is a schematic diagram of the first quick-change head assembly of the intelligent cell preparation workstation.

FIG. 22 is a schematic diagram of the waste liquid and waste material collection mechanism of the intelligent cell preparation workstation.

FIG. 23 is a schematic diagram of a pipette tip assembly of an intelligent cell preparation workstation.

FIG. 24 is a schematic diagram of a cell tube cell collection component of an intelligent cell preparation workstation.

FIG. 25 is a schematic diagram of the cryotube rotary opening assembly of the intelligent cell preparation workstation.

FIG. 26 is a schematic diagram of the cryotube rotary opening assembly of the intelligent cell preparation workstation.

FIG. 27 is a schematic diagram of the cryotube rotary opening assembly of the intelligent cell preparation workstation from another perspective.

FIG. 28 is a schematic diagram of the rotor opening component of the intelligent cell preparation workstation.

FIG. 29 is a schematic diagram of the lifting and fixing plate of the intelligent cell preparation workstation.

FIG30 is a schematic diagram of the feeding mechanism of the intelligent cell preparation workstation.

Figure 31 is a schematic diagram of the centrifugal mechanism of the intelligent cell preparation workstation.

FIG32 is a schematic diagram of a cell culture workstation of an intelligent cell preparation workstation.

FIG33 is a schematic diagram of the interior of the carbon dioxide incubator of the intelligent cell preparation workstation.

FIG. 34 is a schematic diagram showing the interior of the carbon dioxide incubator of the intelligent cell preparation workstation from another perspective.

FIG35 is a schematic diagram of a circular turntable of an intelligent cell preparation workstation.

FIG36 is a schematic diagram of the monitoring and lifting mechanism of the intelligent cell preparation workstation.

FIG37 is a schematic diagram of the clamping mechanism of the intelligent cell preparation workstation.

DETAILED DESCRIPTION

In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and understandable, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

Example

Referring to Figures 1 to 6, the first embodiment of the utility model is provided, which provides an intelligent cell preparation workstation, which includes a cell centrifugal processing station 1, a grabbing and transporting mechanism 2, a cell culture workstation 3, an empty bottle material warehouse 4, and a cell recovery mechanism 5; the cell liquid plate rack assembly 6 can be received by the grabbing and transporting mechanism 2 and placed in the cell recovery mechanism 5 for recovery, and the recovered cell liquid plate rack assembly 6 is placed in the cell centrifugal processing station 1 by the grabbing and transporting mechanism 2 for liquid transfer centrifugation operation, and the cell culture workstation 3 can receive the cell liquid from the cell centrifugal processing station 1 for cell culture; the cell centrifugal processing station 1 can receive empty cell tubes 13105 and empty cell reaction bottles 13205 grabbed by the grabbing and transporting mechanism 2 from the empty bottle material warehouse 4;

Specifically, it includes a cell centrifugation processing station 1, a grabbing and transporting mechanism 2, a cell culture workstation 3, an empty bottle material warehouse 4, and a cell recovery mechanism 5;

A cell centrifugation processing station 1 and a cell recovery mechanism 5 are arranged on one side of the grabbing and transporting mechanism 2, and a cell culture workstation 3 and an empty bottle material warehouse 4 are arranged on the other side;

The grabbing and transporting mechanism 2 can receive the cell liquid rack assembly 6, the cell resuscitation mechanism 5 can receive the cell liquid rack assembly 6 on the grabbing and transporting mechanism 2, and perform cell resuscitation on the cryopreservation tubes in the cell liquid rack assembly 6, the cell centrifugal processing station 1 can receive the resuscitated cell liquid rack assembly 6, and extract the cell liquid in the cryopreservation tubes on the cell liquid rack assembly 6 for pipetting and centrifugation operations; and the cell centrifugal processing station can receive the feed bottle 13304 for supplementary feeding;

The cell culture workstation 3 can receive the cell fluid from the cell centrifugation processing station 1 for cell culture;

The empty bottle material warehouse 4 at least stores empty cell tubes 13105, empty cell reaction bottles 13205 and cryopreservation tubes; the empty bottle material warehouse 4 can provide empty cell tubes 13105, empty cell reaction bottles 13205 and cryopreservation tubes to the cell centrifugation processing station 1 and/or the cell culture workstation through the grabbing and transporting mechanism 2;

The cell centrifugation processing station 1 can receive empty cell tubes 13105 and empty cell reaction bottles 13205 grabbed from the empty bottle storage 4 by the grabbing and transporting mechanism 2.

In summary, the utility model can receive the cell liquid plate rack assembly 6 through the grabbing and transporting mechanism 2, and place it into the cell resuscitation mechanism 5 for resuscitation. The resuscitated cell liquid plate rack assembly 6 can be placed in the cell centrifugal processing station 1, and the cell centrifugal processing station 1 can extract the cells in the cell liquid plate rack assembly 6 for pipetting and centrifugation operations; the cell culture workstation 3 can receive the cell liquid from the cell centrifugal processing station 1 for cell culture; the empty bottle material warehouse 4 can provide empty cell tubes 13105, empty cell reaction bottles 13205 and cryopreservation tubes for the cell centrifugal processing station 1 and/or the cell culture workstation through the grabbing and transporting mechanism 2. The device is fully automated and does not require manual operation, thus saving a lot of manual operation time and avoiding the disadvantages of inaccurate human operation, thereby improving the quality and efficiency of cell culture.

Example

Referring to Figures 1 to 37, the second embodiment of the utility model is shown. In the previous embodiment, the intelligent cell preparation workstation includes a cell centrifugal processing station 1, a grabbing and transporting mechanism 2, a cell culture workstation 3, an empty bottle material warehouse 4, and a cell recovery mechanism 5; the cell liquid plate rack assembly 6 can be received by the grabbing and transporting mechanism 2 and placed in the cell recovery mechanism 5 for recovery, and the recovered cell liquid plate rack assembly 6 is placed in the cell centrifugal processing station 1 by the grabbing and transporting mechanism 2 for liquid transfer and centrifugation operation, and the cell culture workstation 3 can receive the cell liquid from the cell centrifugal processing station 1 for cell culture; the cell centrifugal processing station 1 can receive empty cell tubes 13105 and empty cell reaction bottles 13205 grabbed by the grabbing and transporting mechanism 2 from the empty bottle material warehouse 4;

Specifically, it includes a cell centrifugation processing station 1, a grabbing and transporting mechanism 2, a cell culture workstation 3, an empty bottle material warehouse 4, and a cell recovery mechanism 5;

A cell centrifugation processing station 1 and a cell recovery mechanism 5 are arranged on one side of the grabbing and transporting mechanism 2, and a cell culture workstation 3 and an empty bottle material warehouse 4 are arranged on the other side;

The grabbing and transporting mechanism 2 can receive the cell liquid rack assembly 6, the cell resuscitation mechanism 5 can receive the cell liquid rack assembly 6 on the grabbing and transporting mechanism 2, and perform cell resuscitation on the cryopreservation tubes in the cell liquid rack assembly 6, the cell centrifugal processing station 1 can receive the resuscitated cell liquid rack assembly 6, and extract the cell liquid in the cryopreservation tubes on the cell liquid rack assembly 6 for pipetting and centrifugation operations; and the cell centrifugal processing station can receive the feed bottle 13304 for supplementary feeding;

The cell culture workstation 3 can receive the cell fluid from the cell centrifugation processing station 1 for cell culture;

The empty bottle material warehouse 4 stores at least empty cell tubes 13105, empty cell reaction bottles 13205 and cryopreservation tubes; the empty bottle material warehouse 4 can provide empty cell tubes 13105, empty cell reaction bottles 13205 and cryopreservation tubes to the cell centrifugation processing station 1 and/or the cell culture workstation through the grabbing and transporting mechanism 2;

The cell centrifugation processing station 1 can receive empty cell tubes 13105 and empty cell reaction bottles 13205 grabbed from the empty bottle storage 4 by the grabbing and transporting mechanism 2.

Furthermore, the cell centrifugal processing station 1 includes an upper operating chamber 11, and a centrifugal mechanism 12;

A centrifugal mechanism 12 is provided at the lower end of the upper operating chamber 11;

The upper operating chamber 11 is provided with a suction mechanism 101, a clamping mechanism 102, and a bottle support platform 103;

The bottle support table 103 can be used to place cell tubes 13105, cell reaction bottles 13205 and supply bottles 13304;

The suction mechanism 101 can suck the cell liquid in the cell liquid plate rack assembly 6 and the culture liquid in the feed bottle 13304 into the cell tube 13105, and the clamping mechanism 102 can clamp the cell tube 13105 and put it into the centrifugal mechanism 12 for centrifugation;

The suction mechanism 101 can suck the cell liquid in the cell liquid plate rack assembly 6 and the culture liquid in the supply bottle 13304 into the cell reaction bottle 13205, and the grabbing and transporting mechanism 2 can grab the cell reaction bottle 13205 and put it into the cell culture workstation 3 for cell culture.

Furthermore, a feeding mechanism 104 is provided below the upper operating chamber 11 , and the feeding mechanism 104 can supplement the feeding bottles 13304 on the bottle support platform 103 .

Furthermore, a first manipulator 105 is disposed in the upper operating chamber 11 , and the first manipulator 105 can grab the feeding bottle 13304 on the feeding mechanism 104 and place it on the bottle support platform 103 .

Preferably, the first manipulator 105 is composed of multiple rotation axes, which can realize multi-directional and multi-angle rotation.

Further, the material bottle support platform 103 includes a cell tube support platform 1031, a cell reaction bottle support platform 1032, and a supply bottle support platform 1033;

At least one group of cell tubes 13105 is stored on the cell tube support 1031 , at least one group of cell reaction bottles 13205 is stored on the cell reaction bottle support 1032 , and at least one group of supply bottles 13304 is stored on the supply bottle support 1033 .

Preferably, the cell tube support 1031, the cell reaction bottle support 1032, and the feed bottle support 1033 can all be rotated.

Furthermore, the upper operating chamber 11 also includes a cryotube rotating opening assembly 106 ; the cryotube rotating opening assembly 106 can open the tube caps of the cryotubes on the cell liquid plate rack assembly 6 in the upper operating chamber 11 as a whole plate.

Furthermore, it also includes a cell plate rack in-and-out storage component 7, which can receive an external cell liquid plate rack component 6.

Preferably, the cell plate rack storage and retrieval assembly 7 can be extended to the outside, and retracted into the device after receiving the cell liquid plate rack assembly 6.

Furthermore, it also includes an identification component 8, which can scan and identify the cell liquid plate rack component 6.

Furthermore, the cell tube support platform 1031 includes a cell tube support plate driving member 103101, a cell tube support plate 103102, an upper limit tube slot 103103, a lower limit tube rack 103104, and a limiting rotation member 103106;

The cell tube support disk driving member 103101 is connected to the cell tube support disk 103102. At least one set of upper limit tube grooves 103103 and lower limit tube racks 103104 are provided on the cell tube support disk 103102. The cell tube 13105 can be placed in the upper limit tube grooves 103103 and the lower limit tube racks 103104. The limiting rotating member 103106 can fix the lower end of the cell tube 13105 placed in the lower limit tube rack 103104.

Preferably, the cryotube box support disk driver 601, the feed bottle support disk driver 103302, the cell reaction bottle turntable driver, and the cell tube support disk driver 103101 may be driven by a motor, a cylinder, a hydraulic cylinder, etc., or other drive mechanisms in the prior art.

Preferably, the limiting rotating member 103106 can be driven by a motor or a cylinder, a hydraulic cylinder or other driving mechanism, and then the driving mechanism drives the fixed claw connected thereto to fix the bottom of the cell tube 13105, and then the clamping mechanism 102 rotates the tube cap of the cell tube 13105 to open it.

Preferably, the cell tube support plate driving member 103101 can drive the cell tube support plate 103102 to rotate.

Further, the cell reaction bottle support platform 1032 includes a cell reaction bottle turntable driving member 103201, a cell reaction bottle turntable 103203, and a cell reaction bottle notch 103204;

The cell reaction bottle turntable driver 103201 is connected to the cell reaction bottle turntable 103203. At least one group of cell reaction bottle notches 103204 is arranged on the cell reaction bottle turntable 103203. The cell reaction bottle notches 103204 can store cell reaction bottles 13205. The cell reaction bottle turntable driver 103201 can drive the cell reaction bottle turntable 103203 to rotate.

Furthermore, the supply bottle support platform 1033 includes a supply bottle support plate 103301, a supply bottle support plate driving member 103302, and a storage bottle limiting hole 103303;

The supply bottle support plate driving member 103302 drives the supply bottle support plate 103301 to rotate. At least one group of storage bottle limiting holes 103303 is arranged on the supply bottle support plate 103301, and the supply bottle 13304 can be placed in the storage bottle limiting holes 103303.

It should be noted that the multiple supply bottles 13304 can store four types of liquids, namely complete culture medium, buffer solution, transfer solution and freezing solution, and the barcode scanner will tell the control system the location of each liquid bottle.

Further, the cell liquid plate rack assembly 6 includes a cryotube box support disk driving member 601, a cryotube box support disk 602, a cryotube box support disk rotating shaft 603, and a cryotube box 605;

The cryotube box support disk driver 601 drives the cryotube box support disk 602 to rotate via the cryotube box support disk shaft 603. At least one set of sample box slots 604 is provided on the cryotube box support disk 602. The sample box slots 604 can store cryotube boxes 605, and the cryotube boxes 605 can store cryotubes.

Preferably, the cryotube box support disk driver 601 can drive the cryotube box support disk 602 to rotate, so that one group of cryotube boxes 605 can be rotated to the bottom side of the cryotube rotation opening assembly 106 to open the cryotube caps as a whole.

Furthermore, the upper operating chamber 11 also includes an upper connecting frame 107 ; the upper connecting frame 107 is disposed at the upper end of the upper operating chamber 11 , and an upper sliding guide rail 108 is disposed on the upper connecting frame 107 , and the suction mechanism 101 and the clamping mechanism 102 can move along the upper sliding guide rail 108 .

Further, the suction mechanism 101 includes a first upper horizontal sliding guide member 10101, a first upper vertical guide member 10102, a second slider 10103, a suction head driving mechanism 10104, a suction head connecting groove 10105, and a suction head 11102;

The first upper horizontal sliding guide member 10101 is slidably connected to the upper sliding guide rail 108, the first upper vertical guide member 10102 is slidably connected to the first upper horizontal sliding guide member 10101, the second slider 10103 is vertically slidably connected to the first upper vertical guide member 10102, the second slider 10103 is connected to the suction head driving mechanism 10104, and the suction head connecting groove 10105 is connected to the suction head 11102.

Preferably, the suction head driving mechanism 10104 and the suction head 11102 can realize XYZ axis movement to facilitate suction and pipetting.

Further, the clamping mechanism 102 includes a second upper horizontal sliding rail 10201, a second upper vertical sliding rail 10202, a first driving member 10203, a first slider 10204, a third upper vertical sliding rail 10205, a second driving member 10207, a rotating clamping portion 10208, and a first connecting plate 10209;

The second upper horizontal sliding guide rail 10201 is slidably connected to the upper sliding guide rail 10208, the second upper vertical sliding guide rail 10202 is slidably connected to the second upper horizontal sliding guide rail 10201, the second upper vertical sliding guide rail 10202 is provided with a first driving member 10203, the first driving member 10203 can drive the first slider 10204 to slide vertically along the second upper vertical sliding guide rail 10202, the third upper vertical sliding guide rail 10205 is connected to the first slider 10204, the first connecting plate 10209 is vertically slidably connected to the second upper vertical sliding guide rail 10202, the first connecting plate 10209 is provided with a second driving member 10207, and the second driving member 10207 is connected to the rotating clamping portion 10208.

Preferably, the second driving member 10207 and the rotating clamping portion 10208 can realize movement of the XYZ axis, making it convenient to grasp and move the cell tube 13105.

It should be noted that the driving members in this embodiment may be motors, electric cylinders, air cylinders, hydraulic cylinders, or existing driving mechanisms in the prior art.

Preferably, the second driving member 10207 can rotate the clamping part 10208 to grab and move the cell tube 13105, and can also drive the rotating clamping part 10208 to rotate and unscrew the tube cap of the cell tube 13105.

Furthermore, the upper operating chamber 11 also includes a first quick-change head assembly 109 . The first quick-change head assembly 109 can allow the first manipulator 105 to replace different grippers. The first quick-change head assembly 109 includes at least one group of grippers.

Preferably, the first quick-change head assembly 109 can be provided with a variety of grippers, so that the first manipulator 105 can grab the cryotube, the cell tube 13105, the cell reaction bottle 13205 and the feed bottle 13304 by simply replacing the corresponding grippers;

Furthermore, the cryotube rotary opening assembly 106 includes a vertical support seat 10601, a first driving lifting mechanism 10602, a second driving lifting mechanism 10603, a rotary opening member 10604, a lifting fixing plate 10605, a sensor 10606; and a rotary through hole 10607;

A first driving lifting mechanism 10602 and a second driving lifting mechanism 10603 are provided on the vertical support seat 10601. The first driving lifting mechanism 10602 can drive the rotary head opening member 10604 to be lifted and lowered, and the second driving lifting mechanism 10603 can drive the lifting and fixing plate 10605 to be lifted and lowered. The sensor 10606 is connected to the lifting and fixing plate 10605, and a rotary head through hole 10607 is opened on the lifting and fixing plate 10605.

Preferably, the first drive lifting mechanism 10602 includes a first motor and a first screw rod; the second drive lifting mechanism 10603 may include a second motor and a second screw rod; the first motor can drive the first screw rod to rotate, and the first screw rod drives the rotary head opening piece 10604 to lift and lower, and the first screw rod and the rotary head opening piece 10604 are threadedly connected; the second motor drives the second screw rod to rotate, and the second screw rod drives the lifting and fixing plate 10605 to lift and lower, and the second screw rod and the lifting and fixing plate 10605 are threadedly connected; the rotary head opening piece 10604 and the lifting and fixing plate 10605 are both lifted and lowered along the guide rail on the side of the vertical support seat 10601.

Further, the rotary opening member 10604 includes a first side support plate 1060401, a second middle support plate 1060402, a first rotating wheel 1060403, a second rotating wheel 1060404, a rotating belt 1060405, a transmission gear 1060406, a rotating shaft 1060407, a spring 1060408, a connecting tooth 1060409, and a cover opening rotary head 1060410;

The first side support plates 1060401 are connected to both sides of the second middle support plate 1060402, a top plate is arranged above the second middle support plate 1060402, the first rotating wheel 1060403 is connected to the second rotating wheel 1060404 through a rotating belt 1060405, the inner side of the rotating belt 1060405 is meshed and connected with the transmission gear 1060406, the transmission gear 1060406 is connected to the rotating shaft 1060407, the spring 1060408 is arranged on the outer side of the rotating shaft 1060407, and the lower end of the rotating shaft 1060407 is connected to the cover opening rotating head 1060410 through a connecting tooth 1060409.

Preferably, the first rotating wheel 1060403 can be driven to rotate by a motor, and the first rotating wheel 1060403 is connected to the second rotating wheel 1060404 through a rotating belt 1060405, and the inner side of the rotating belt 1060405 can drive the transmission gear 1060406 to rotate, and the transmission gear 1060406 drives the rotating shaft 1060407 to rotate, and the rotating shaft 1060407 drives the opening lid rotor 1060410 to rotate through the connecting teeth 1060409, and the opening lid rotor 1060410 can be inserted into the jar lid of the cryogenic tube, so as to open the tube lids of multiple groups of cryogenic tubes as a whole.

Furthermore, the centrifugal mechanism 12 includes a centrifuge 121 and a centrifuge support frame 122; the centrifuge support frame 122 supports the centrifuge 121;

At least one group of centrifugal slots is provided in the centrifuge 121 , and the cell tube 13105 can be placed in the centrifugal slots. The centrifuge 121 can centrifuge the cell tube 13105 .

Preferably, the clamping mechanism 102 can clamp the cell tube 13105 and place it into the centrifuge 121 for centrifugation. After the centrifugation is completed, the cell tube 13105 is placed on the cell tube support table 1031 again.

Furthermore, the upper operation chamber 11 also includes a cell tube cell collection component 110 , and the cell tube cell collection component 110 can collect cells after centrifugation of the cell tube 13105 .

Further, the feeding mechanism 104 includes a loading movable base 10401, a loading driving member 10402, a moving track 10403, a loading movable plate 10404, a loading turntable driving member 10405, a loading turntable 10406, and a bottle storage limiting hole 103303;

A loading driving member 10402 is provided on the loading movable base 10401, and a moving track 10403 is provided on the upper end of the loading driving member 10402. The loading driving member 10402 can drive the loading movable plate 10404 to slide along the moving track 10403. The upper end of the loading movable plate 10404 is connected to the loading turntable driving member 10405. The loading turntable driving member 10405 can drive the loading turntable 10406 to rotate. At least one group of storage bottle limiting holes 103303 is provided on the loading turntable 10406, and the supply bottles 13304 are placed in the storage bottle limiting holes 103303.

Preferably, the first manipulator 105 can grab the supply bottle 13304 on the loading turntable 10406 and place it on the supply bottle support platform 1033 for standby use.

Furthermore, a first laminar flow hood 911 is arranged above the upper operating chamber 11 , and a second laminar flow hood 912 is arranged above the first laminar flow hood 911 , the cell culture workstation 3 , and the empty bottle material warehouse 4 .

Preferably, the clean laminar flow hood forms a uniform flow layer after the air passes through the high-efficiency filter at a certain wind speed, so that the clean air flows vertically in one direction, thereby ensuring that the cleanliness required by the process is achieved in the working area.

Preferably, the first laminar flow hood 911 adopts a Class 100 clean laminar flow hood, which is a device that creates a local high-cleanliness environment in a low-cleanliness environment, and can make the local working environment reach Class 100. It can independently form a return air system or be connected to a pipeline system, with a flexible purification area, and is equipped with a low-noise centrifugal fan and a shock absorbing device. It is installed in a suspended or bracket-supported form. The box body is made of high-quality 304 stainless steel, with a solid structure and a safe and reliable electrical system. It is widely used in electronics, medicine, national defense, science and technology, bioengineering and other fields, and is an ideal equipment for achieving high purification level requirements in low-purification areas.

Preferably, the second laminar flow hood 912 adopts a Class 10,000 laminar flow hood with high purification efficiency and reaches the Class 10,000 cleanliness standard.

Furthermore, the upper operating chamber 11 also includes a suction head assembly 111 and a counterweight assembly 112; a counterweight assembly 112 is arranged on the side of the suction head assembly 111, the suction head assembly 111 includes at least one group of suction heads 11102, and the counterweight assembly 112 is provided with at least one group of counterweights.

Preferably, a plurality of groups of suction heads 11102 are arranged in the suction head assembly 111, and each time the suction head 11102 sucks liquid, the suction head needs to be replaced to ensure the purity of the liquid without contamination.

Preferably, the counterweight assembly 112 is provided with at least one set of counterweights, and the counterweights can be placed in the centrifuge, or placed in the cell tube support 1031 to maintain balance.

Furthermore, the upper operating chamber 11 also includes a waste liquid and waste material collection mechanism 115, which collects waste water or waste material.

Furthermore, the waste liquid and waste material collection mechanism 115 includes a first waste liquid collection port 11501, a second waste liquid collection port 11502, a waste material docking port 11503, a waste liquid storage tank 11504, and a waste material storage tank 11505;

The first waste liquid collecting port 11501, the second waste liquid collecting port 11502 and the waste docking port 11503 are all arranged in the upper operating chamber 11, and the waste liquid storage tank 11504 and the waste storage tank 11505 are arranged below the upper operating chamber 11. The first waste liquid collecting port 11501 and the second waste liquid collecting port 11502 are discharged into the waste liquid storage tank 11504 through a pipe, and the waste docking port 11503 is discharged into the waste storage tank 11505 through a pipe.

Furthermore, a front flap door mechanism 116 and a rear sealing door mechanism 117 are respectively provided on the two side walls of the upper operating chamber 11 .

Preferably, the front door and the rear door can be opened by the front flip door mechanism 116 and the rear sealing door mechanism 117 .

Furthermore, the grabbing and transporting mechanism 2 includes a second manipulator 201 and a second manipulator track 202;

The bottom end of the second robot arm 201 is slidably connected to the second robot arm track 202 .

Preferably, the second robot 201 can slide along the second robot track 202 under the action of the motor.

Furthermore, a second quick-change head assembly 203 is disposed at a side end of the second robot rail 202 , and at least one set of quick-change heads 204 is disposed on the second quick-change head assembly 203 .

Preferably, the second quick-change head assembly is provided with multiple sets of quick-change heads 204, which can open the upper cover of the transfer tank, the grippers of the cryopreservation tubes, cell tubes 13105, cell reaction bottles 13205, and the cryopreservation tube box 605, and the second manipulator 201 can be quickly replaced.

Furthermore, the cell resuscitation mechanism 5 includes a lifting and pressing component 51 and a resuscitation sample slot 52;

The lifting and pressing assembly 51 can immerse the cell liquid plate rack assembly 6 in the recovery sample tank 52 .

Furthermore, the lifting and lowering pressing assembly 51 includes a pressing support member 5101, a first horizontal driving member 5102, a second vertical driving member 5103, a sample box support limit plate 5104, a sample box pressing plate 5105, and a pressing plate driving member 5106;

A first horizontal driving member 5102 is arranged at the upper end of the pressing support member 5101, and the first horizontal driving member 5102 is connected to the second vertical driving member 5103, and the second vertical driving member 5103 is connected to the sample box support limiting plate 5104, and a pressing plate driving member 5106 is arranged on the sample box support limiting plate 5104, and the pressing plate driving member 5106 drives the sample box pressing plate 5105 to rise and fall.

Preferably, the first horizontal driving member 5102 can drive the second vertical driving member 5103 to move horizontally, and the second vertical driving member 5103 can drive the sample box supporting limiting plate 5104 to move up and down.

Preferably, the cryotube box 605 can be placed on the sample box support limit plate 5104, and the sample box pressing plate 5105 can press the cryotube box 605 to prevent the cryotube from loosening due to water pressure.

Furthermore, a heating element 53, a temperature measuring element 54, and an inlet and outlet pipe 55 are provided in the resuscitation sample tank 52;

The liquid inlet and outlet pipes 55 can inject or discharge liquid into the resuscitation sample tank 52, the heating element 53 can heat the liquid, and the temperature measuring element 54 can measure the temperature of the liquid.

Furthermore, the cell culture workstation 3 includes a carbon dioxide incubator 31 and an incubator door opening and closing mechanism 32;

An incubator door opening and closing mechanism 32 is provided on the side of the carbon dioxide incubator 31 , and the carbon dioxide incubator 31 can store at least one group of cell reaction bottles 13205 . The transport mechanism 2 can grab the cell reaction bottles 13205 inside the carbon dioxide incubator 31 through the incubator door opening and closing mechanism 32 .

Furthermore, at least one group of circular turntables 3101 is arranged in the carbon dioxide incubator 31, and a monitoring and lifting mechanism 3102 is arranged on the side of the circular turntable 3101. The monitoring and lifting mechanism 3102 can monitor the internal state of the cell reaction bottle 13205 and can grab it; at least one group of cell reaction bottles 13205 can be stored on the circular turntable 3101.

Furthermore, a rotating shaft 3103 is provided in the carbon dioxide incubator 31 , and multiple groups of circular turntables 3101 are vertically arranged around the rotating shaft 3103 .

Furthermore, the multiple groups of circular turntables 3101 can rotate individually or simultaneously.

Furthermore, the carbon dioxide incubator 31 also includes a turntable driving mechanism 3104 ; the turntable driving mechanism 3104 is disposed on the inner wall of the carbon dioxide incubator 31 , and the turntable driving mechanism 3104 can drive the circumferential turntable 3101 to rotate around the rotation axis 3103 .

Furthermore, each set of circular turntables 3101 is correspondingly provided with a turntable driving mechanism 3104 .

Furthermore, a cell reaction bottle notch 103204 is provided on the circumferential turntable 3101 , side limit blocks 3106 are provided on both sides of the cell reaction bottle notch 103204 , and a top limit block 3107 is provided in front of the side limit block 3106 at one end close to the rotating shaft 3103 .

Further, the monitoring and lifting mechanism 3102 includes a visual observation mechanism 310201, a clamping mechanism 310202, and a clamping driving member 310203;

The clamping drive 310203 can drive the visual observation mechanism 310201 and the clamping mechanism 310202 to rise and fall, which is to facilitate observation and clamping. The clamping mechanism 310202 can support the visual observation mechanism 310201, and the clamping mechanism 310202 can grab the cell reaction bottle 13205 on the circular turntable 3101.

Preferably, the visual observation mechanism 310201 includes an upper observation camera and a lower observation camera, which facilitates the monitoring of the cell reaction bottle 13205.

Preferably, the clamping mechanism 310202 is composed of an upper clamping plate and a lower clamping plate, and the upper clamping plate and the lower clamping plate clamp the cell reaction bottle 13205. The upper clamping plate and the lower clamping plate can be driven by a motor or other driving mechanisms in the prior art.

Preferably, the upper clamping plate and the lower clamping plate can support the upper observation camera and the lower observation camera.

Preferably, the clamping drive member 310203 can be composed of a driving mechanism of the prior art, such as a motor and a screw rod, or other driving lifting mechanisms of the prior art.

Furthermore, the empty bottle material warehouse 4 includes at least one group of supporting material plates 401, on which the cell reaction bottles 13205, the cell tubes 13105 and the cryopreservation tubes can be placed.

Furthermore, multiple groups of supporting material plates 401 can be arranged vertically.

Furthermore, the supporting plate 401 can be self-rotating or fixed.

Furthermore, the supporting plate 401 includes a first supporting plate 4011 and a second supporting plate 4012;

A second support plate 4012 is disposed at the upper end of the first support plate 4011 . The first support plate 4011 can be used to place empty cell reaction bottles 13205 , and the second support plate 4012 can be used to place empty cryopreservation tubes and empty cell tubes 13105 .

Furthermore, the grabbing and transferring mechanism 2 can grab the target empty bottle on the supporting material plate 401 and place it into the upper operating chamber 11 .

Furthermore, it also includes a transfer tank conveying mechanism 211, which is arranged on the side of the empty bottle warehouse 4. The transfer tank conveying mechanism 211 can receive external transfer tanks, and the grabbing and transferring mechanism 2 can open the can lid of the transfer tank.

During use, 1. The experimenter presses the equipment start button, and the first laminar flow hood 911 and the second laminar flow hood 912 start working, so that the internal area of the entire workstation reaches the 10,000-level cleanliness standard, and the cell centrifugation processing station 1 reaches the 100-level cleanliness standard; 2. The experimenter presses the loading button, and the feeding mechanism 104 extends outward. The experimenter places four culture fluids on the feeding mechanism 104. After the loading is completed, the experimenter presses the loading button again, and the feeding mechanism 104 retracts; 3. The experimenter presses the plate rack input button, and the cell plate rack in-and-out storage component 7 extends. The experimenter places the cell liquid plate rack component 6 with the cell freezing solution on the plate rack placement position of the cell plate rack in-and-out storage component 7, and the experimenter presses the plate rack input button again. The cell plate rack in-and-out storage component 7 is retracted; 4. The experimenter presses the transfer tank input button, the transfer tank conveying mechanism 211 extends, and the experimenter places the transfer tank in the transfer tank placement position. The experimenter presses the transfer tank input button again and the transfer tank conveying mechanism 211 retracts; 5. The grabbing and transporting mechanism 2 grabs the cell liquid plate rack component 6, moves it above the identification component 8, and the barcode scanner scans the code. After the scanning is completed, the grabbing and transporting mechanism 2 places the cell liquid plate rack component 6 on the sample box support limit plate 5104 of the cell recovery mechanism 5, and the sample box pressing plate 5105 will After the cryopreservation tube box 605 of the cell liquid plate rack assembly 6 is pressed, the whole is moved downward to immerse the cryopreservation tube box 605 of the cell liquid plate rack assembly 6 in 37°C pure water and then moved horizontally left and right. After the cryopreservation tubes in the plate rack are revived, the sample box support limit plate 5104 is moved out of the water surface, and the sample box pressing plate 5105 rises; 6. The grabbing and transporting mechanism 2 puts the revived cell liquid plate rack assembly 6 on the cryopreservation tube box support plate 602 in the upper operating chamber 11; 7. The grabbing and transporting mechanism 2 puts the empty cell reaction bottles 13205 and the cell reaction bottles 13205 in the empty bottle warehouse 4 10. The cell tube 13105 is placed on the corresponding cell tube support 1031 and the cell reaction bottle support 1032. During this period, the grabbing and transporting mechanism 2 replaces the gripper to grab the corresponding bottle; 8. The clamping mechanism 102 can open the lid of the cell tube 13105 and the cell reaction bottle 13205; 9. The suction mechanism 101 sucks 1ml of seed cells containing freezing solution in the freezing tube, and then places it in the cell tube 13105, and then sucks 9ml of complete culture medium in the feed bottle 13304 again and places it in the cell tube 13105, and then the clamping mechanism 1 02 Clamp the cell tube 13105, tighten the tube cap, and place it in the centrifuge mechanism 12 for centrifugation; 10. After the centrifugation is completed, the clamping mechanism 102 clamps the cell tube 13105 and places it on the cell tube support table 1031, then unscrews the tube cap, and the suction mechanism 101 sucks the excess waste liquid and pours it out, and then the suction mechanism 101 sucks the complete culture medium in the supply bottle 13304 again to resuspend the precipitate; 11. The suction mechanism 101 sucks the complete culture medium in the supply bottle 13304 and places it in 3 cell reaction bottles 13205, each cell reaction bottle Bottle 13205 requires 30 ml of complete culture medium; 12. The aspirating mechanism 101 aspirates the cell solution in the cryopreservation tube in the cell solution plate rack assembly 6 and adds it to the three cell reaction bottles 13205. Each cell reaction bottle 13205 requires 5 ml, and then the clamping mechanism 102 tightens the bottle cap; 13. The grabbing and transporting mechanism 2 grabs the three cell reaction bottles 13205 from the cell reaction bottle support table 1032 respectively, shakes them evenly for 10 seconds, and places them on the circumferential turntable 3101 in the cell culture workstation 3; 14. Repeat steps 1-13 , so that the carbon dioxide incubator 31 is filled with 90 cell reaction bottles 13205 containing cells; 15. The visual observation mechanism 310201 in the carbon dioxide incubator 31 can observe the growth status of the cells in the cell reaction bottle 13205, and the cells that have grown well can be clamped out by the clamping mechanism 310202, and then the grabbing and transporting mechanism 2 places the cell reaction bottle 13205 clamped out by the clamping mechanism 310202 on the cell reaction bottle support table 1032; 16. Then pour out 35ml of the culture medium, and then suck it out by suction. The taking mechanism 101 sucks 10 ml of buffer solution from the feed bottle 13304 and puts it into the cell reaction bottle 13205. The grabbing and transporting mechanism 2 shakes the cell reaction bottle 13205 and puts it on the cell reaction bottle support table 1032. Then the sucking mechanism 101 sucks the waste liquid and discharges it. 17. Then the sucking mechanism 101 sucks 5 ml of cell culture solution from the feed bottle 13304 and puts it into each cell reaction bottle 13205, and keeps it still for 1 to 2 minutes. After the generation cells fall off, the sucking mechanism 101 sucks 5 ml of complete culture solution from the feed bottle 13304. The culture medium terminates the digestion reaction; 18. The first manipulator 105 grabs the cell reaction bottle 13205 and shakes it evenly, then pours 10 ml of liquid from each bottle into a 50 ml cell tube 13105 to merge multiple bottles of cells; 19. Then the clamping mechanism 102 puts the cell tube 13105 into the centrifugal mechanism 12 for centrifugation. After centrifugation, the suction mechanism 101 sucks away the waste liquid in the cell tube 13105 and flows the cell precipitate; 20. The cell tube 13105 containing the cells is grabbed by the grabbing and transporting mechanism 2 and placed into the transfer tank. The device issues a command to take out the transfer tank.

Importantly, it should be noted that the construction and arrangement of the present application shown in a number of different exemplary embodiments are only exemplary. Although only a few embodiments are described in detail in this disclosure, it should be readily understood by those who refer to this disclosure that many modifications are possible (e.g., the size, scale, structure, shape and proportion of various elements, and parameter values (e.g., temperature, pressure, etc.), mounting arrangements, use of materials, color, directional changes, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in the application. For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of the element may be inverted or otherwise changed, and the nature or number or position of the discrete element may be changed or changed. Therefore, all such modifications are intended to be included in the scope of the present utility model. The order or sequence of any process or method steps may be changed or reordered according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure described herein that performs the function, and is not only structurally equivalent but also an equivalent structure. Without departing from the scope of the present invention, other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments. Therefore, the present invention is not limited to a specific embodiment, but extends to various modifications that still fall within the scope of the appended claims.

Additionally, in order to provide a concise description of example embodiments, all features of an actual embodiment may not be described (ie, those features that are not relevant to the best mode presently contemplated for carrying out the invention or those that are not relevant to implementing the invention).

It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.

It should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model is described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims (45)

1. An intelligent cell preparation workstation, characterized in that it comprises a cell centrifugation processing station (1), a grabbing and transporting mechanism (2), a cell culture workstation (3), an empty bottle material warehouse (4), and a cell recovery mechanism (5); A cell centrifugation processing station (1) and a cell recovery mechanism (5) are arranged on one side of the grabbing and transporting mechanism (2), and a cell culture workstation (3) and an empty bottle material warehouse (4) are arranged on the other side; The grabbing and transporting mechanism (2) can receive the cell liquid rack assembly (6), the cell resuscitation mechanism (5) can receive the cell liquid rack assembly (6) on the grabbing and transporting mechanism (2), and perform cell resuscitation on the cryopreservation tubes in the cell liquid rack assembly (6), the cell centrifugal processing station (1) can receive the resuscitated cell liquid rack assembly (6), and extract the cell liquid in the cryopreservation tubes on the cell liquid rack assembly (6) for pipetting and centrifugation operations; and the cell centrifugal processing station can receive a feed bottle (13304) for supplementary feeding; The cell culture workstation (3) can receive the cell fluid from the cell centrifugation processing station (1) to perform cell culture; The empty bottle material warehouse (4) at least stores empty cell tubes (13105), empty cell reaction bottles (13205) and cryotubes; the empty bottle material warehouse (4) can provide empty cell tubes (13105), empty cell reaction bottles (13205) and cryotubes for the cell centrifugation processing station (1) and/or the cell culture workstation through the grabbing and transporting mechanism (2); The cell centrifugation processing station (1) can receive empty cell tubes (13105) and empty cell reaction bottles (13205) grabbed from the empty bottle storage (4) by the grabbing and transporting mechanism (2). 2. The intelligent cell preparation workstation according to claim 1, characterized in that: the cell centrifugal processing station (1) comprises an upper operating chamber (11) and a centrifugal mechanism (12); A centrifugal mechanism (12) is provided at the lower end of the upper operating chamber (11); The upper operating chamber (11) is provided with a suction mechanism (101), a clamping mechanism (102), and a bottle support platform (103); The material bottle support platform (103) can be used to place cell tubes (13105), cell reaction bottles (13205) and supply bottles (13304); The suction mechanism (101) can suck the cell fluid in the cell fluid plate rack assembly (6) and the culture fluid in the supply bottle (13304) into the cell tube (13105), and the clamping mechanism (102) can clamp the cell tube (13105) and place it into the centrifugal mechanism (12) for centrifugation; The suction mechanism (101) can suck the cell fluid in the cell fluid rack assembly (6) and the culture fluid in the supply bottle (13304) into the cell reaction bottle (13205), and the grabbing and transporting mechanism (2) can grab the cell reaction bottle (13205) and place it in the cell culture workstation (3) for cell culture. 3. The intelligent cell preparation workstation as described in claim 2 is characterized in that a feeding mechanism (104) is arranged below the upper operating chamber (11), and the feeding mechanism (104) can replenish the feeding bottle (13304) on the bottle support platform (103). 4. The intelligent cell preparation workstation as described in claim 3 is characterized in that a first manipulator (105) is arranged in the upper operating chamber (11), and the first manipulator (105) can grab the supply bottle (13304) on the supply mechanism (104) and place it on the bottle support platform (103). 5. The intelligent cell preparation workstation according to claim 2, characterized in that: the material bottle support platform (103) includes a cell tube support platform (1031), a cell reaction bottle support platform (1032), and a supply bottle support platform (1033); At least one group of cell tubes (13105) is stored on the cell tube support platform (1031), at least one group of cell reaction bottles (13205) is stored on the cell reaction bottle support platform (1032), and at least one group of supply bottles (13304) is stored on the supply bottle support platform (1033). 6. The intelligent cell preparation workstation as described in claim 2 is characterized in that: the upper operating chamber (11) also includes a cryotube rotating opening component (106); the cryotube rotating opening component (106) can open the tube caps of the cryotubes on the cell liquid plate rack component (6) in the upper operating chamber (11). 7. The intelligent cell preparation workstation as described in claim 1 is characterized by: further comprising a cell plate rack storage and retrieval component (7), wherein the cell plate rack storage and retrieval component (7) can receive an external cell liquid plate rack component (6). 8. The intelligent cell preparation workstation according to claim 1, characterized in that it also includes an identification component (8), and the identification component (8) can scan and identify the cell liquid plate rack component (6). 9. The intelligent cell preparation workstation according to claim 5, characterized in that: the cell tube support platform (1031) comprises a cell tube support plate driving member (103101), a cell tube support plate (103102), an upper limit tube slot (103103), a lower limit tube rack (103104), and a limiting rotation member (103106); The cell tube support disk driving member (103101) is connected to the cell tube support disk (103102), and the cell tube support disk (103102) is provided with at least one group of upper limit tube grooves (103103) and lower limit tube racks (103104). The cell tube (13105) can be placed in the upper limit tube grooves (103103) and the lower limit tube racks (103104), and the limiting rotation member (103106) can fix the lower end of the cell tube (13105) placed in the lower limit tube rack (103104). 10. The intelligent cell preparation workstation according to claim 5, characterized in that: the cell reaction bottle support platform (1032) comprises a cell reaction bottle turntable drive (103201), a cell reaction bottle turntable (103203), and a cell reaction bottle notch (103204); The cell reaction bottle turntable driving component (103201) is connected to the cell reaction bottle turntable (103203); at least one group of cell reaction bottle notches (103204) is arranged on the cell reaction bottle turntable (103203); cell reaction bottles (13205) can be stored in the cell reaction bottle notches (103204); the cell reaction bottle turntable driving component (103201) can drive the cell reaction bottle turntable (103203) to rotate. 11. The intelligent cell preparation workstation according to claim 5, characterized in that: the feed bottle support platform (1033) comprises a feed bottle support plate (103301), a feed bottle support plate driving member (103302), and a storage bottle limiting hole (103303); The supply bottle support plate driving member (103302) drives the supply bottle support plate (103301) to rotate, and at least one group of storage bottle limiting holes (103303) is arranged on the supply bottle support plate (103301), and the supply bottle (13304) can be placed in the storage bottle limiting holes (103303). 12. The intelligent cell preparation workstation according to claim 1, characterized in that: the cell liquid plate rack assembly (6) comprises a cryotube box support disk drive (601), a cryotube box support disk (602), a cryotube box support disk rotating shaft (603), and a cryotube box (605); The cryotube box support disk driving member (601) drives the cryotube box support disk (602) to rotate via the cryotube box support disk rotating shaft (603), and at least one group of sample box slots (604) are provided on the cryotube box support disk (602), and cryotube boxes (605) can be stored in the sample box slots (604), and cryotubes can be stored in the cryotube boxes (605). 13. The intelligent cell preparation workstation as described in claim 2 is characterized in that: the upper operating chamber (11) also includes an upper connecting frame (107); an upper connecting frame (107) is arranged at the upper end of the upper operating chamber (11), and an upper sliding guide rail (108) is arranged on the upper connecting frame (107), and the suction mechanism (101) and the clamping mechanism (102) can move along the upper sliding guide rail (108). 14. The intelligent cell preparation workstation according to claim 13, characterized in that: the suction mechanism (101) comprises a first upper horizontal sliding guide member (10101), a first upper vertical guide member (10102), a second slider (10103), a suction head driving mechanism (10104), a suction head connecting groove (10105), and a suction head (11102); The first upper horizontal sliding guide member (10101) is slidably connected to the upper sliding guide rail (108), the first upper vertical guide member (10102) is slidably connected to the first upper horizontal sliding guide member (10101), the second slider (10103) is vertically slidably connected to the first upper vertical guide member (10102), the second slider (10103) is connected to the suction head driving mechanism (10104), and the suction head connecting groove (10105) is connected to the suction head (11102). 15. The intelligent cell preparation workstation according to claim 13, characterized in that: the clamping mechanism (102) comprises a second upper horizontal sliding rail (10201), a second upper vertical sliding rail (10202), a first driving member (10203), a first slider (10204), a third upper vertical sliding rail (10205), a second driving member (10207), a rotating clamping portion (10208), and a first connecting plate (10209); The second upper horizontal sliding guide rail (10201) is slidably connected to the upper sliding guide rail (108), the second upper vertical sliding guide rail (10202) is slidably connected to the second upper horizontal sliding guide rail (10201), the second upper vertical sliding guide rail (10202) is provided with a first driving member (10203), the first driving member (10203) can drive the first sliding block (10204) to slide vertically along the second upper vertical sliding guide rail (10202), the third upper vertical sliding guide rail (10205) is connected to the first sliding block (10204), the first connecting plate (10209) is vertically slidably connected to the second upper vertical sliding guide rail (10202), the first connecting plate (10209) is provided with a second driving member (10207), and the second driving member (10207) is connected to the rotating clamping portion (10208). 16. The intelligent cell preparation workstation as described in claim 4 is characterized in that: the upper operating chamber (11) also includes a first quick-change head assembly (109), and the first quick-change head assembly (109) can allow the first manipulator (105) to replace different clamps, and the first quick-change head assembly (109) includes at least one group of clamps. 17. The intelligent cell preparation workstation according to claim 6, characterized in that: the cryotube rotary opening assembly (106) comprises a vertical support seat (10601), a first drive lifting mechanism (10602), a second drive lifting mechanism (10603), a turret opening member (10604), a lifting fixing plate (10605), a sensor (10606); and a turret through hole (10607); The vertical support seat (10601) is provided with a first driving lifting mechanism (10602) and a second driving lifting mechanism (10603); the first driving lifting mechanism (10602) can drive the rotary head opening member (10604) to be lifted and lowered; the second driving lifting mechanism (10603) can drive the lifting and fixing plate (10605) to be lifted and lowered; the sensor (10606) is connected to the lifting and fixing plate (10605); and a rotary head through hole (10607) is provided on the lifting and fixing plate (10605). 18. The intelligent cell preparation workstation according to claim 17, characterized in that: the rotor opening member (10604) comprises a first side support plate (1060401), a second middle support plate (1060402), a first rotating wheel (1060403), a second rotating wheel (1060404), a rotating belt (1060405), a transmission gear (1060406), a rotating shaft (1060407), a spring (1060408), a connecting tooth (1060409), and a cover opening rotor (1060410); The first side support plates (1060401) are connected to both sides of the second middle support plate (1060402), a top plate is arranged above the second middle support plate (1060402), the first rotating wheel (1060403) and the second rotating wheel (1060404) are connected through the rotating belt (1060405), the inner side of the rotating belt (1060405) is meshed and connected with the transmission gear (1060406), the transmission gear (1060406) is connected with the rotating shaft (1060407), the spring (1060408) is arranged on the outer side of the rotating shaft (1060407), and the lower end of the rotating shaft (1060407) is connected to the cover opening rotating head (1060410) through the connecting teeth (1060409). 19. The intelligent cell preparation workstation according to claim 2, characterized in that: the centrifugal mechanism (12) comprises a centrifuge (121) and a centrifuge support frame (122); the centrifuge support frame (122) supports the centrifuge (121); At least one group of centrifugal slots is arranged in the centrifuge (121), and the cell tubes (13105) can be placed in the centrifugal slots. The centrifuge (121) can centrifuge the cell tubes (13105). 20. The intelligent cell preparation workstation as described in claim 2 is characterized in that: the upper operation chamber (11) also includes a cell tube cell collection component (110), and the cell tube cell collection component (110) can collect cells after centrifugation of the cell tube (13105). 21. The intelligent cell preparation workstation according to claim 3, characterized in that: the feeding mechanism (104) comprises a loading movable base (10401), a loading driving member (10402), a moving track (10403), a loading movable plate (10404), a loading turntable driving member (10405), a loading turntable (10406), and a storage bottle limiting hole (103303); The loading movable base (10401) is provided with a loading driving member (10402), and the upper end of the loading driving member (10402) is provided with a moving track (10403), and the loading driving member (10402) can drive the loading movable plate (10404) to slide along the moving track (10403), and the upper end of the loading movable plate (10404) is connected to the loading turntable driving member (10405), and the loading turntable driving member (10405) can drive the loading turntable (10406) to rotate, and at least one group of storage bottle limiting holes (103303) is provided on the loading turntable (10406), and the supply bottles (13304) are placed in the storage bottle limiting holes (103303). 22. The intelligent cell preparation workstation as described in claim 2 is characterized in that a first laminar flow hood (911) is arranged above the upper operating chamber (11), and a second laminar flow hood (912) is arranged above the first laminar flow hood (911), the cell culture workstation (3), and the empty bottle material warehouse (4). 23. The intelligent cell preparation workstation as described in claim 2 is characterized in that: the upper operating chamber (11) also includes a tip assembly (111) and a counterweight assembly (112); a counterweight assembly (112) is arranged on the side of the tip assembly (111), the tip assembly (111) includes at least one group of tips (11102), and the counterweight assembly (112) is provided with at least one group of counterweights. 24. The intelligent cell preparation workstation as described in claim 2 is characterized in that: the upper operating chamber (11) also includes a waste liquid and waste material collection mechanism (115), and the waste liquid and waste material collection mechanism (115) collects waste water or waste material. 25. The intelligent cell preparation workstation according to claim 24, characterized in that: the waste liquid and waste material collection mechanism (115) comprises a first waste liquid collection port (11501), a second waste liquid collection port (11502), a waste material docking port (11503), a waste liquid storage tank (11504), and a waste material storage tank (11505); The first waste liquid collection port (11501), the second waste liquid collection port (11502) and the waste docking port (11503) are all arranged in the upper operating chamber (11), and the waste liquid storage tank (11504) and the waste storage tank (11505) are arranged below the upper operating chamber (11), and the first waste liquid collection port (11501) and the second waste liquid collection port (11502) are discharged into the waste liquid storage tank (11504) through a pipeline, and the waste docking port (11503) is discharged into the waste storage tank (11505) through a pipeline. 26. The intelligent cell preparation workstation as described in claim 2 is characterized in that a front flap door mechanism (116) and a rear sealing door mechanism (117) are respectively provided on the two side walls of the upper operation chamber (11). 27. The intelligent cell preparation workstation according to claim 1, characterized in that: the grabbing and transporting mechanism (2) comprises a second manipulator (201) and a second manipulator track (202); The bottom end of the second robot (201) is slidably connected to the second robot track (202). 28. The intelligent cell preparation workstation as described in claim 27 is characterized in that a second quick-change head assembly (203) is arranged at the side end of the second robot rail (202), and at least one group of quick-change heads (204) is arranged on the second quick-change head assembly (203). 29. The intelligent cell preparation workstation according to claim 1, characterized in that: the cell recovery mechanism (5) comprises a lifting and pressing component (51) and a recovery sample slot (52); The lifting and pressing assembly (51) can immerse the cell liquid plate rack assembly (6) in the resuscitation sample tank (52). 30. The intelligent cell preparation workstation according to claim 29, characterized in that: the lifting and pressing assembly (51) comprises a pressing support member (5101), a first horizontal driving member (5102), a second vertical driving member (5103), a sample box support limit plate (5104), a sample box pressing plate (5105), and a pressing plate driving member (5106); A first horizontal driving member (5102) is arranged at the upper end of the pressing support member (5101), and the first horizontal driving member (5102) is connected to the second vertical driving member (5103), and the second vertical driving member (5103) is slidably connected to the sample box support and limiting plate (5104), and a pressing plate driving member (5106) is arranged on the sample box support and limiting plate (5104), and the pressing plate driving member (5106) drives the sample box pressing plate (5105) to rise and fall. 31. The intelligent cell preparation workstation according to claim 30, characterized in that: a heating element (53), a temperature measuring element (54), and an inlet and outlet pipe (55) are provided in the resuscitation sample tank (52); The liquid inlet and outlet pipe (55) can inject or discharge liquid into the resuscitation sample tank (52), the heating element (53) can heat the liquid, and the temperature measuring element (54) can measure the temperature of the liquid. 32. The intelligent cell preparation workstation according to claim 1, characterized in that: the cell culture workstation (3) comprises a carbon dioxide incubator (31) and an incubator door opening and closing mechanism (32); An incubator door opening and closing mechanism (32) is arranged on the side of the carbon dioxide incubator (31); the carbon dioxide incubator (31) can store at least one group of cell reaction bottles (13205); and the fetching and transporting mechanism (2) can grab the cell reaction bottles (13205) inside the carbon dioxide incubator (31) through the incubator door opening and closing mechanism (32). 33. The intelligent cell preparation workstation as described in claim 32 is characterized in that: at least one group of circular turntables (3101) are arranged in the carbon dioxide incubator (31), and a monitoring and lifting mechanism (3102) is arranged on the side of the circular turntable (3101), and the monitoring and lifting mechanism (3102) can monitor the internal state of the cell reaction bottle (13205) and can grab it; at least one group of cell reaction bottles (13205) can be stored on the circular turntable (3101). 34. The intelligent cell preparation workstation as described in claim 33 is characterized in that a rotating shaft (3103) is arranged in the carbon dioxide incubator (31), and multiple groups of the circular turntables (3101) are vertically arranged around the rotating shaft (3103). 35. The intelligent cell preparation workstation as described in claim 33 or 34, characterized in that: the multiple groups of circular turntables (3101) can rotate individually or simultaneously. 36. The intelligent cell preparation workstation as described in claim 35 is characterized in that: the carbon dioxide incubator (31) also includes a turntable driving mechanism (3104); a turntable driving mechanism (3104) is arranged on the inner wall of the carbon dioxide incubator (31), and the turntable driving mechanism (3104) can drive the circular turntable (3101) to rotate around the rotation axis (3103). 37. The intelligent cell preparation workstation as described in claim 36 is characterized in that each of the circular turntables (3101) is correspondingly provided with a turntable driving mechanism (3104). 38. The intelligent cell preparation workstation as described in claim 37 is characterized in that: a cell reaction bottle slot (103204) is opened on the circular turntable (3101), side limit blocks (3106) are arranged on both sides of the cell reaction bottle slot (103204), and a top limit block (3107) is arranged at one end in front of the side limit block (3106) close to the rotating shaft (3103). 39. The intelligent cell preparation workstation according to claim 38, characterized in that: the monitoring and lifting mechanism (3102) comprises a visual observation mechanism (310201), a clamping mechanism (310202), and a clamping driving member (310203); The clamping drive (310203) can drive the visual observation mechanism (310201) and the clamping mechanism (310202) to rise and fall, the clamping mechanism (310202) can support the visual observation mechanism (310201), and the clamping mechanism (310202) can grab the cell reaction bottle (13205) on the circular turntable (3101). 40. The intelligent cell preparation workstation as described in claim 2 is characterized in that: the empty bottle material warehouse (4) includes at least one group of supporting material plates (401), and the cell reaction bottles (13205), cell tubes (13105) and cryopreservation tubes can be placed on the supporting material plates (401). 41. The intelligent cell preparation workstation as described in claim 40 is characterized in that: multiple groups of the supporting material plates (401) can be arranged vertically. 42. The intelligent cell preparation workstation as described in claim 41 is characterized in that the supporting material plate (401) can be rotated or fixed. 43. The intelligent cell preparation workstation according to any one of claims 40 to 42, characterized in that: the support plate (401) comprises a first support plate (4011) and a second support plate (4012); A second support plate (4012) is disposed at the upper end of the first support plate (4011), and the first support plate (4011) can be used to place empty cell reaction bottles (13205), and the second support plate (4012) can be used to place empty cryopreservation tubes and empty cell tubes (13105). 44. The intelligent cell preparation workstation as described in claim 43 is characterized in that: the grabbing and transporting mechanism (2) can grab the target empty bottle on the supporting material plate (401) and place it in the upper operating chamber (11). 45. The intelligent cell preparation workstation as described in claim 1 is characterized by: it also includes a transfer tank conveying mechanism (211), the transfer tank conveying mechanism (211) is arranged on the side of the empty bottle material warehouse (4), the transfer tank conveying mechanism (211) can receive an external transfer tank, and the grabbing and transferring mechanism (2) can open the tank cover of the transfer tank.