CN221166547U - Nucleic acid detection equipment - Google Patents

Abstract

The application is applicable to the technical field of biological detection, and provides nucleic acid detection equipment which comprises an operation platform, a loading module, a consumable module, an upper adsorption extraction module, a lower adsorption extraction module, a detection module and a sample loading pipetting module, wherein the modules are independent from each other, and each module can be detachably arranged at the corresponding position of the operation platform; the modularized design makes the nucleic acid detecting equipment easier to maintain and repair, and because different modules are mutually independent, the normal operation of the equipment can be restored by only replacing the damaged module, and the whole equipment is not required to be replaced or repaired; meanwhile, the upper adsorption extraction module and the lower adsorption extraction module can be mutually replaced, so that material difference is reduced, and parallel production is realized.

Description

Nucleic acid detection equipment

Technical Field

The application belongs to the technical field of biological detection, and particularly relates to nucleic acid detection equipment.

Background

The substance detected by the nucleic acid is a viral nucleic acid to determine whether it is infected by the virus by looking for the presence of foreign invading viral nucleic acid in the patient's respiratory tract specimen, blood or stool. Nucleic acid detection equipment is required for detecting nucleic acids.

At present, nucleic acid extraction and detection integrated equipment generally comprises a driving module, a PCR module, a consumable module, a feeding module, a material taking module and the like, and the overall structure is complex. When one of the modules is damaged, the function of the damaged module cannot be normally exerted, the whole extraction and detection process may be affected, and for the production line production, time and maintenance are required, which causes production delay and affects the production efficiency and the yield.

Meanwhile, the nucleic acid extraction method which is widely applied to the existing nucleic acid extraction and detection integrated equipment is a magnetic bead method, and the magnetic bead method is divided into a lower magnetic attraction method and an upper magnetic attraction method according to the positions of the magnetic beads. The lower magnetic nucleic acid detection device or the upper magnetic nucleic acid detection device has low overall modularization degree and complex structure. In addition, the material difference between the lower magnetic nucleic acid extraction and detection and the upper magnetic nucleic acid extraction and detection is large, the parallel production cannot be realized, and the industrialization degree is low.

Disclosure of utility model

The application provides a nucleic acid detection device, which can solve the problems that the existing nucleic acid detection device is complex in overall structure, low in modularization degree, large in material difference between lower magnetic nucleic acid extraction detection and upper magnetic nucleic acid extraction detection, and incapable of achieving parallel production.

The application provides nucleic acid detection equipment which comprises an operation platform, a loading module, a consumable module, an upper adsorption extraction module, a lower adsorption extraction module, a detection module and a loading pipetting module, wherein the loading module is connected with the consumable module;

The operation platform is provided with a loading position, a consumable position, a nucleic acid extraction position and a nucleic acid detection position, the loading module is detachably arranged at the loading position, the consumable module is detachably arranged at the consumable position, any one of the upper adsorption extraction module and the lower adsorption extraction module is detachably arranged at the nucleic acid extraction position, the detection module is detachably arranged at the nucleic acid detection position, the loading pipetting module is detachably arranged on the operation platform, and the loading pipetting module is used for transferring liquid among the loading position, the consumable position, the nucleic acid extraction position and the nucleic acid detection position.

Optionally, the upper adsorption extraction module and the lower adsorption extraction module are respectively provided with a first shell and a first filter assembly, and the detection module is provided with a second shell and a second filter assembly;

The exhaust port of the first filter element of the upper or lower adsorption extraction module located at the nucleic acid extraction site and the exhaust port of the second filter element of the detection module located at the nucleic acid detection site are oriented in different directions, respectively.

Optionally, the operation platform is provided with a first area, a second area and a third area, the loading position and the consumable position are located in the first area, the nucleic acid extraction position is located in the second area, the nucleic acid detection position is located in the third area, and the second area and the third area are located on opposite or adjacent two sides of the first area respectively.

Optionally, the first shell of the upper adsorption extraction module is detachably arranged on the operation platform, the upper adsorption extraction module further comprises an upper magnetic attraction mechanism and a first deep pore plate carrier which are arranged in the first shell, the first deep pore plate carrier is loaded with a first deep pore plate, and the upper magnetic attraction mechanism can adsorb, transfer and release the magnetic beads in the first deep pore plate from the upper part.

Optionally, the first shell of the lower adsorption extraction module is detachably arranged on the operation platform, the lower adsorption extraction module further comprises a lower magnetic attraction mechanism, a second deep pore plate carrier and a waste liquid removing mechanism which are arranged in the first shell, the second deep pore plate carrier is loaded with the second deep pore plate, the lower magnetic attraction mechanism is arranged below the second deep pore plate carrier, a magnetic attraction piece of the lower magnetic attraction mechanism can be close to or far away from the second deep pore plate so as to adsorb magnetic beads in the second deep pore plate to the side wall or the bottom of the second deep pore plate and remove the adsorption of the magnetic beads in the second deep pore plate, and the waste liquid removing mechanism is used for transferring the waste liquid in the second deep pore plate.

Optionally, the detachable setting of second shell is provided with a plurality ofly on operating platform, and a plurality of second filter components all set up in the second shell, and detection module still includes a plurality of PCR appearance that set up in the second shell, a plurality of PCR appearance and a plurality of second filter components one-to-one's setting.

Optionally, the application of sample pipetting module includes the installing frame, set up actuating mechanism on the installing frame and set up the imbibition mechanism on actuating mechanism, and the installing frame can detachably install on operating platform, and imbibition mechanism can adsorb and draw any one of the module under and in the module, application of sample module, consumable module and detection module under the actuating mechanism ground and make a round trip movement to realize the transfer of liquid.

Optionally, the sample loading module comprises a sample tube carrier and a bar code scanner, the sample tube carrier is used for loading sample tubes, the sample tube carrier and the bar code scanner are detachably arranged on the operation platform, the bar code scanner can scan the sample tubes on the sample tube carrier one by one, and experimental information of each sample tube is recorded.

Optionally, the consumable module includes:

the consumable bearing plate is detachably arranged on the operation platform;

the PCR tube carrier is arranged on the consumable carrier plate, and the PCR tube is arranged on the PCR tube carrier;

The reagent tank carrier is arranged on the consumable carrier plate, and the reagent tube is arranged on the reagent tank carrier; and

The gun head carrier and gun head, the gun head carrier is arranged on the consumable carrier plate, and the gun head is arranged on the gun head carrier.

Optionally, the loading position, the consumable position, the nucleic acid extraction position and the nucleic acid detection position are respectively provided with a positioning track, the loading module, the consumable module and the detection module are respectively detachably mounted on the corresponding positioning tracks, and any one of the upper adsorption extraction module and the lower adsorption extraction module can be detachably mounted on the corresponding positioning track.

The scheme of the application has the following beneficial effects:

The nucleic acid detection equipment provided by the application comprises an operation platform, a sample loading module, a consumable module, an upper adsorption extraction module, a lower adsorption extraction module, a detection module and a sample loading pipetting module, wherein the modules are independent from each other, and each module can be detachably arranged at a corresponding position of the operation platform; the modularized design makes the nucleic acid detecting equipment easier to maintain and repair, and because different modules are mutually independent, the normal operation of the equipment can be restored by only replacing the damaged module, and the whole equipment is not required to be replaced or repaired; meanwhile, the upper adsorption extraction module and the lower adsorption extraction module can be mutually replaced, so that material difference is reduced, and parallel production is realized.

Other advantageous effects of the present application will be described in detail in the detailed description section which follows.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments or the description of the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a nucleic acid detecting apparatus according to an embodiment of the present application;

FIG. 2 is a schematic diagram showing a portion of a nucleic acid detecting apparatus according to an embodiment of the present application;

FIG. 3 is a schematic diagram of an upper adsorption extraction module according to an embodiment of the present application;

FIG. 4 is a schematic diagram of a lower adsorption extraction module according to an embodiment of the present application;

FIG. 5 is a schematic diagram of a detection module according to an embodiment of the present application;

FIG. 6 is a schematic diagram of a sample module according to an embodiment of the present application;

FIG. 7 is a schematic diagram of a consumable module according to an embodiment of the present application;

fig. 8 is a schematic structural diagram of a sample addition pipetting module according to an embodiment of the application.

[ Reference numerals description ]

1. An operating platform; 11. a first region; 12. a second region; 13. a third region;

2. a loading module; 21. a sample tube carrier; 22. a bar code scanner;

3. A consumable module; 31. a consumable carrier plate; 32. a PCR tube carrier; 33. a reagent tank carrier; 34. a gun head carrier; 35. a waste tank;

401. A first housing; 402. a first filter assembly;

41. An upper adsorption extraction module; 411. a magnetic attraction mechanism is arranged; 4111. a frame; 4112. a lateral movement mechanism; 4113. a first driving member; 4114. a second driving member; 4115. a magnetic rod; 4116. a magnetic rod sleeve; 412. a first deep aperture plate carrier; 413. a first deep well plate;

42. A lower adsorption extraction module; 421. a lower magnetic attraction mechanism; 422. a second deep aperture plate carrier; 423. a waste liquid removal mechanism; 424. a second deep well plate; 425. heating and vibrating mechanism;

5. A detection module; 501. a second housing; 502. a second filter assembly; 503. a PCR instrument;

6. A sample adding and pipetting module; 61. a mounting frame; 62. a driving mechanism; 621. a lateral pipetting mechanism; 622. a longitudinal pipetting mechanism; 623. a front-back pipetting mechanism; 63. a liquid suction mechanism.

Detailed Description

In the following description, for purposes of explanation and not limitation, specific details are set forth such as the particular system architecture, techniques, etc., in order to provide a thorough understanding of the embodiments of the present application. It will be apparent, however, to one skilled in the art that the present application may be practiced in other embodiments that depart from these specific details. In other instances, detailed descriptions of well-known systems, devices, circuits, and methods are omitted so as not to obscure the description of the present application with unnecessary detail.

It should be understood that the terms "comprises" and/or "comprising," when used in this specification and the appended claims, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

It should also be understood that the term "and/or" as used in the present specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations.

As used in the present description and the appended claims, the term "if" may be interpreted as "when..once" or "in response to a determination" or "in response to detection" depending on the context. Similarly, the phrase "if a determination" or "if a [ described condition or event ] is detected" may be interpreted in the context of meaning "upon determination" or "in response to determination" or "upon detection of a [ described condition or event ]" or "in response to detection of a [ described condition or event ]".

Furthermore, the terms "first," "second," "third," and the like in the description of the present specification and in the appended claims, are used for distinguishing between descriptions and not necessarily for indicating or implying a relative importance.

Reference in the specification to "one embodiment" or "some embodiments" or the like means that a particular feature, structure, or characteristic described in connection with the embodiment is included in one or more embodiments of the application. Thus, appearances of the phrases "in one embodiment," "in some embodiments," "in other embodiments," and the like in the specification are not necessarily all referring to the same embodiment, but mean "one or more but not all embodiments" unless expressly specified otherwise. The terms "comprising," "including," "having," and variations thereof mean "including but not limited to," unless expressly specified otherwise.

The nucleic acid detecting apparatus provided by the present application will be exemplarily described below with reference to specific examples.

As shown in fig. 1, an embodiment of the present application provides a nucleic acid detecting apparatus, which includes an operation platform 1, an up sample module 2, a consumable module 3, an up adsorption and extraction module 41, a down adsorption and extraction module 42, a detection module 5, and a sample addition pipetting module 6; the sample loading position, the consumable position, the nucleic acid extraction position and the nucleic acid detection position are arranged on the operation platform 1, the sample loading module 2 is detachably arranged at the sample loading position, the consumable module 3 is detachably arranged at the consumable position, any one of the upper adsorption extraction module 41 and the lower adsorption extraction module 42 is detachably arranged at the nucleic acid extraction position, the detection module 5 is detachably arranged at the nucleic acid detection position, the sample loading pipetting module 6 is detachably arranged on the operation platform 1, and the sample loading pipetting module 6 is used for transferring liquid among the sample loading position, the consumable position, the nucleic acid extraction position and the nucleic acid detection position.

The nucleic acid detection equipment provided by the application comprises an operation platform 1, an upper sample module 2, a consumable module 3, an upper adsorption extraction module 41, a lower adsorption extraction module 42, a detection module 5 and a sample adding pipetting module 6, wherein the modules are respectively independent, and each module can be detachably arranged at the corresponding position of the operation platform 1; the modularized design makes the nucleic acid detecting equipment easier to maintain and repair, and because the different modules are independent of each other, the normal operation of the equipment can be restored by only replacing the damaged module, and the whole equipment is not required to be replaced or repaired. Meanwhile, the upper adsorption extraction module 41 and the lower adsorption extraction module 42 can be mutually replaced, so that material difference is reduced, and parallel production is realized.

Furthermore, the modular design also facilitates upgrades and modifications of the nucleic acid detecting apparatus, which can be accomplished by replacing or adding appropriate modules without redesigning and manufacturing the entire apparatus if it is necessary to update certain functions of the apparatus or to add new functions.

It will be appreciated that in the above embodiments, the sample loading module 2 is mainly used for storing and collecting information about the sample tubes. The consumable module 3 is mainly used for storing consumables required in the nucleic acid extraction detection process, including, but not limited to, a pipette tip, reagents (e.g., lysate, washing buffer, etc.), PCR tubes, etc. The upper adsorption extraction module 41 extracts nucleic acids in the sample mainly by an upper magnetic attraction method, and the lower adsorption extraction module 42 extracts nucleic acids in the sample mainly by a lower magnetic attraction method. The detection module 5 is mainly used for detecting the extracted nucleic acid. The sample adding and pipetting module 6 is mainly used for moving among a sample loading position, a consumable position, a nucleic acid extracting position and a nucleic acid detecting position so as to realize liquid transfer.

In the existing nucleic acid detecting apparatus, the nucleic acid extracting mechanism and the nucleic acid detecting mechanism (i.e., PCR mechanism) are generally disposed at different positions of the rack, but the two mechanisms are not isolated or the flow path of the air flow is planned therebetween, which easily causes cross contamination of the nucleic acid extracting and PCR reagents due to aerosol escape, thereby affecting the detection result.

To this end, in one embodiment, as shown in fig. 2 and 5, the upper and lower adsorption extraction modules 41 and 42 each have a first housing 401 and a first filter assembly 402, and the detection module 5 has a second housing 501 and a second filter assembly 502; the exhaust port of the first filter assembly 402 of the upper or lower adsorption extraction module 41 or 42 located at the nucleic acid extraction site and the exhaust port of the second filter assembly 502 of the detection module 5 located at the nucleic acid detection site are oriented in different directions, respectively.

In the above embodiment, the first filter assembly 402 is disposed in the first housing 401, the air outlet of the first filter assembly 402 is disposed on the first housing 401, the second filter assembly 502 is disposed in the second housing 501, and the air outlet of the second filter assembly 502 is disposed on the second housing 501. By providing the first filter assembly 402 and the second filter assembly 502, the gas exhausted from the first housing and the second housing is filtered to prevent contamination of the outside air. Meanwhile, the exhaust port of the first filter assembly 402 and the exhaust port of the second filter assembly 502 are respectively oriented in different directions, so that cross contamination is avoided. By way of example, the first filter assembly 402 and the second filter assembly 502 may be a screen, a fan assembly, or the like.

In a specific embodiment, as shown in fig. 2, the operation platform 1 has a first region 11, a second region 12 and a third region 13, where the loading position and the consumable position are located in the first region 11, the nucleic acid extraction position is located in the second region 12, the nucleic acid detection position is located in the third region 13, and the second region 12 and the third region 13 are located on opposite or adjacent sides of the first region 11, respectively.

In the above embodiment, aerosols generated in the first region 11, the second region 12 and the third region 13 flow to different positions, respectively, avoiding cross contamination. The upper adsorption extraction module 41 or the lower adsorption extraction module 42 on the nucleic acid extraction site moves in different directions with the gas exhausted from the exhaust port of the first filter assembly 402, the gas exhausted from the exhaust port of the second filter assembly 502 by the detection module 5 on the nucleic acid detection site, and the gas exhausted from the sample loading module 2 on the sample loading site and the gas exhausted from the consumable module 3 on the consumable site, respectively, so as to avoid cross contamination. For example, when the second region 12 and the third region 13 are located on opposite sides of the first region 11, respectively, since the gas generated by the loading module 2 at the loading position and the consumable module 3 at the consumable position is naturally discharged upwards, the exhaust port of the first filter assembly 402 faces to one side far from the first region 11, and the exhaust port of the second filter assembly 502 faces to the other side far from the first region 11, the discharge direction of the gas forms an inverted "T" (as shown by the dotted arrow in fig. 2), so as to avoid cross contamination. The discharge direction may have other forms similar to the inverted "T" form described above, and is not limited herein.

In a specific embodiment, as shown in fig. 2 and fig. 3, the first housing 401 of the upper adsorption and extraction module 41 is detachably disposed on the operation platform 1, the upper adsorption and extraction module 41 further includes an upper magnetic attraction mechanism 411 and a first deep hole plate carrier 412 disposed in the first housing 401, the first deep hole plate carrier 412 carries the first deep hole plate 413 thereon, and the upper magnetic attraction mechanism 411 can adsorb, transfer and release the magnetic beads in the first deep hole plate 413 from above.

In the above embodiment, after the sample in the first row of wells in the first deep well plate 413 is lysed (it can be understood that the first row of wells is pre-transferred with the lysate and the sample by the sample-adding pipetting module 6), the magnetic beads in the first row of wells in the first deep well plate 413 are adsorbed (i.e. the nucleic acids in the lysate are extracted) by the upper magnetic attraction mechanism 411, and then transferred to the second row of wells (the second row of wells is pre-loaded with the washing solution by the sample-adding pipetting module 6), and the adsorption of the magnetic beads is released, thereby realizing the transfer of the nucleic acids into the washing solution for purification.

In a more specific embodiment, as shown in fig. 3, the upper magnetic attraction mechanism 411 includes a frame 4111, a lateral movement mechanism 4112, a first driving member 4113, a second driving member 4114, a magnetic rod 4115, and a magnetic rod housing 4116; the frame 4111 is disposed in the first housing 401, the lateral moving mechanism 4112 is disposed on the frame 4111, the first driving member 4113 and the second driving member 4114 are both disposed on the driving end of the lateral moving mechanism 4112, the magnetic rod housing 4116 is disposed on the driving end of the first driving member 4113, and the magnetic rod 4115 is disposed on the driving end of the second driving member 4114; the first driving member 4113 is configured to drive the magnetic rod housing 4116 to insert into or withdraw from one of the rows of holes in the first deep hole plate 413; the second driving member 4114 is configured to drive the magnetic rod 4115 to insert into or withdraw from the magnetic rod housing 4116; the lateral moving mechanism 4112 is configured to move the first driving member 4113 and the second driving member 4114 along the direction of each row of holes in the first deep hole plate 413.

In the above embodiment, when the sample in the first row of holes in the first deep hole plate 413 is completely lysed (it will be understood that the first row of holes is pre-transferred with the lysate and the sample by the loading pipetting module 6), the first driving member 4113 drives the magnetic rod housing 4116 to descend until the magnetic rod housing 4116 is inserted into the first row of holes in the first deep hole plate 413. And, the second driving member 4114 drives the magnetic rod 4115 to descend until it is inserted into the magnetic rod housing 4116, so that the magnetic beads in the row of holes are adsorbed on the magnetic rod housing 4116 (i.e. nucleic acid in the lysate is extracted). Then, the first driving member 4113 and the second driving member 4114 are lifted together until the bar magnet housing 4116 exits the first row of holes of the first deep hole plate 413. Then, the lateral moving mechanism 4112 drives the first driving member 4113 and the second driving member 4114 to move together along the directions of the rows of holes of the first deep hole plate 413 until the magnetic rod housing 4116 and the magnetic rod 4115 are aligned with the second row of holes of the first deep hole plate 413 (at this time, the magnetic rod 4115 remains inserted into the magnetic rod housing 4116). Then, the first driving member 4113 and the second driving member 4114 are lowered together until the magnetic rod housing 4116 having the magnetic beads adsorbed thereon is inserted into the second row of holes (it will be understood that the second row of holes is filled with the washing liquid in advance by the pipetting module 6). Then, the second driving member 4114 drives the magnetic rod 4115 to lift until the magnetic rod 4115 exits the magnetic rod housing 4116, thereby removing the adsorption of the magnetic beads, transferring the nucleic acid into the washing solution for purification, adding the eluent to separate the magnetic beads from the nucleic acid, transferring the magnetic beads through the above process, and finally transferring the nucleic acid in the second row of holes through the sample adding pipetting module 6.

It will be appreciated that the first driving member 4113 may reciprocate rapidly, so as to drive the magnetic rod housing 4116 to oscillate in the first row of holes and the second row of holes in the vertical direction, thereby providing the oscillating operation required in the nucleic acid extraction process.

In a specific embodiment, as shown in fig. 2 and fig. 4, the first housing 401 of the lower adsorption and extraction module 42 is detachably disposed on the operation platform 1, the lower adsorption and extraction module 42 further includes a lower magnetic attraction mechanism 421 disposed in the first housing 401, a second deep-hole plate carrier 422, and a waste liquid removing mechanism 423, the second deep-hole plate carrier 422 carries a second deep-hole plate 424, the lower magnetic attraction mechanism 421 is disposed below the second deep-hole plate carrier 422, and a magnetic attraction member of the lower magnetic attraction mechanism 421 can be close to or far from the second deep-hole plate 424 to adsorb magnetic beads in the second deep-hole plate 424 to a side wall or a bottom of the second deep-hole plate 424, and desorb the magnetic beads in the second deep-hole plate 424, and the waste liquid removing mechanism 423 is used for transferring the waste liquid in the second deep-hole plate 424.

In the above embodiment, after the sample in the first row of wells in the second deep well plate 424 is lysed (it will be understood that the first row of wells is pre-transferred with the lysate and the sample by the sample application pipetting module 6), the magnetic attraction member of the lower magnetic attraction mechanism 421 is close to the second deep well plate 424, so that the magnetic beads in the first row of wells are adsorbed to the side wall or the bottom of the first row of wells in the second deep well plate 424 (i.e. nucleic acids in the lysate are extracted), and then the waste liquid in the first row of wells is removed by the waste liquid removing mechanism 423. Then, washing (removing impurities) and eluting (separating magnetic beads from nucleic acids) are performed in the first row of wells by the above procedure, the operation steps are identical to those of the above procedure, which will not be described again, and finally, nucleic acids in the second row of wells are transferred by the sample addition pipetting module 6.

In a more specific embodiment, the lower adsorption extraction module 42 further includes a heating and shaking mechanism 425, and the heating and shaking mechanism 425 is used to provide the temperature switching, shaking operation, etc. required in the nucleic acid extraction process.

In one embodiment, as shown in fig. 2 and fig. 5, the second housing 501 is detachably disposed on the operation platform 1, the second filter assemblies 502 are provided in plurality, and the second filter assemblies 502 are disposed in the second housing 501, and the detection module 5 further includes a plurality of PCR instruments 503 disposed in the second housing 501, where the PCR instruments 503 are disposed in a one-to-one correspondence with the second filter assemblies 502.

In the above embodiment, the PCR instrument 503 is used to detect the extracted nucleic acid, and the second filter assembly 502 is used to filter the gas exhausted from the second housing 501 to prevent air pollution. The PCR instrument 503 and the second filter assembly 502 are provided with a plurality of filters to improve efficiency. The PCR instrument 503 may be an existing PCR detection device, and is not limited herein.

In one embodiment, as shown in fig. 8, the sample application pipetting module 6 includes a mounting frame 61 provided on the operation platform 1, a driving mechanism 62 provided on the mounting frame 61, and a pipetting mechanism 63 provided on the driving mechanism 62, the mounting frame 61 being detachably mountable on the operation platform 1, the pipetting mechanism 63 being movable back and forth between any one of the upper suction extraction module 41 and the lower suction extraction module 42, the sample application module 2, the consumable module 3, and the detection module 5 under the action of the driving mechanism 62 to effect transfer of liquid.

In the above-described embodiment, the pipetting mechanism 63 is capable of moving back and forth between any one of the upper and lower adsorption extraction modules 41 and 42, the sample loading module 2, the consumable module 3, and the detection module 5 by the drive mechanism 62 to effect transfer of liquid during nucleic acid detection.

In a specific embodiment, as shown in FIG. 8, the drive mechanism 62 includes a lateral pipetting mechanism 621, a longitudinal pipetting mechanism 622, and a front-to-back pipetting mechanism 623; the pipetting mechanism 63 may be a liquid-sucking pump that can move back and forth between any one of the upper suction extraction module 41 and the lower suction extraction module 42, the sample loading module 2, the consumable module 3, and the detection module 5 in cooperation with the lateral pipetting mechanism 621, the longitudinal pipetting mechanism 622, and the front-rear pipetting mechanism 623.

In the above embodiments, the liquid suction pump is mainly used for generating negative pressure to suck liquid and positive pressure to discharge liquid. The lateral pipetting mechanism 621, the longitudinal pipetting mechanism 622 and the front-to-back pipetting mechanism 623 are mainly used for driving a liquid suction pump to move so as to complete the processes of gun loading, sample transferring, reagent (including but not limited to lysate, washing solution and the like) adding, nucleic acid extracting solution transferring and the like in the nucleic acid detection process.

In a specific embodiment, the drive mechanism 62 further includes a gripping mechanism and an uncapping mechanism (not shown); the operation platform 1 is also provided with a clamping position, the clamping mechanism is detachably arranged at the clamping position, and the clamping mechanism is provided with a clamping part for clamping or loosening the sample tube; the operation platform 1 is also provided with a cover opening position, the cover opening mechanism is arranged at the cover opening position and is used for transferring the sample tube between the sample loading position and the clamping part and opening or closing the cover of the sample tube clamped at the clamping part.

In the above embodiment, the cap opening mechanism is moved onto the loading module 2, and grips the sample tube. Then, the cap opening mechanism is moved to the holding position of the holding mechanism, and the held sample tube is placed in the holding position of the holding mechanism. The clamping part of the clamping mechanism clamps the sample tube positioned at the clamping position. At this time, the cap opening mechanism unscrews (i.e., opens) the tube cap of the sample tube, and the liquid absorbing mechanism 63 moves to the holding position of the holding mechanism and absorbs the sample liquid in the sample tube located at the holding position. Next, the liquid suction mechanism 63 moves to any one of the upper suction extraction module 41 and the lower suction extraction module 42, and releases the sucked liquid. Any one of the upper adsorption extraction module 41 and the lower adsorption extraction module 42 performs nucleic acid extraction on the liquid. At the same time, the cap opening mechanism screws the tube cap onto the sample tube again (i.e., cap). The clamping part of the clamping mechanism releases the sample tube, and the cover opening mechanism transfers the sample tube to the sample carrier again. The above steps are sequentially circulated until all the liquid in the sample tubes on the sample carrier are transferred into the nucleic acid extraction mechanism, respectively, so that any one of the upper and lower adsorption extraction modules 41 and 42 performs nucleic acid extraction on each sample, respectively.

In one embodiment, as shown in fig. 6, the sample loading module 2 includes a sample tube carrier 21 and a barcode scanner 22, the sample tube carrier 21 is used for loading sample tubes, the sample tube carrier 21 and the barcode scanner 22 are detachably disposed on the operation platform 1, and the barcode scanner 22 can scan the sample tubes on the sample tube rack one by one and record experimental information of each sample tube.

In the above embodiment, the sample tube carrier 21 is detachably disposed on the operation platform 1, and the sample tube carrier 21 can reciprocate on the operation platform 1 along the direction parallel to the barcode scanner 22, so that the sample tubes can be scanned one by the barcode scanner 22, and the scanning information can be in one-to-one correspondence with the detection results, so that not only can automatic unified management be performed, but also independent analysis can be performed on the detection results.

In one embodiment, as shown in fig. 7, the consumable module 3 includes a consumable carrier plate 31, a PCR tube carrier 32 and PCR tubes, a reagent tank carrier 33 and reagent tubes, and a gun head carrier 34 and gun heads; the consumable bearing plate 31 is detachably arranged on the operation platform 1; the PCR tube carrier 32 is arranged on the consumable carrier plate 31, and the PCR tube is arranged on the PCR tube carrier 32; the reagent tank carrier 33 is arranged on the consumable carrier plate 31, and the reagent tube is arranged on the reagent tank carrier 33; the gun head carrier 34 is disposed on the consumable carrier 31, and the gun head is disposed on the gun head carrier 34.

In the above embodiment, the consumable carrier plate 31 is detachably disposed on the operation platform 1, and the entire consumable module 3 can be replaced by detaching the consumable carrier plate 31. The PCR tube is used to store the liquid extracted from the upper or lower adsorption extraction modules 41 or 42, and then the PCR tube may be transferred to the detection module 5 through the sample addition pipetting module 6 for nucleic acid detection, and the PCR tube may be an eight-tube, for example. The reagent tube is used to store reagents required in the nucleic acid extraction detection process, including but not limited to lysis solution, wash buffer, and the like. The gun head frame can provide gun heads of different specifications to be placed, and the gun heads are selected according to different project requirements, so that diversity selection is provided for experiments, and the gun heads are disposable consumables for sample adding and pipetting for the sample adding pipetting module 6.

In a specific embodiment, as shown in fig. 7, the consumable module 3 further includes a waste tank 35, where the waste tank 35 is disposed on the consumable carrier plate 31, and the waste tank 35 is used for collecting consumable waste after use.

In one embodiment, the loading position, the consumable position, the nucleic acid extraction position and the nucleic acid detection position are respectively provided with a positioning track, and the loading module 2, the consumable module 3 and the detection module 5 are respectively detachably mounted on the corresponding positioning tracks, and any one of the upper adsorption extraction module 41 and the lower adsorption extraction module 42 can be detachably mounted on the corresponding positioning track.

In the above-described embodiment, the detachable mounting of any one of the upper suction extraction module 41 and the lower suction extraction module 42, the sample loading module 2, the consumable module 3, and the detection module 5 on the corresponding positions of the operation platform 1 is achieved by the positioning rails.

In a specific embodiment, either one of the upper suction extraction module 41 and the lower suction extraction module 42 is detachably mounted on a corresponding positioning rail through the first housing 401. The sample tube carriers 21 in the sample module 2 are detachably and movably connected to corresponding positioning rails. The consumable module 3 is detachably mounted on the corresponding positioning rail through the consumable carrier plate 31. The detection modules 5 are detachably mounted on the corresponding positioning rails through the second housing 501.

While the foregoing is directed to the preferred embodiments of the present application, it will be appreciated by those skilled in the art that various modifications and adaptations can be made without departing from the principles of the present application, and such modifications and adaptations are intended to be comprehended within the scope of the present application.

Claims (10)

1. The nucleic acid detection equipment is characterized by comprising an operation platform (1), an upper sample module (2), a consumable module (3), an upper adsorption and extraction module (41), a lower adsorption and extraction module (42), a detection module (5) and a sample adding and pipetting module (6);

The sample loading and removing device is characterized in that the sample loading position, the consumable position, the nucleic acid extraction position and the nucleic acid detection position are arranged on the operation platform (1), the sample loading module (2) is detachably arranged at the sample loading position, the consumable module (3) is detachably arranged at the consumable position, any one of the upper adsorption and extraction module (41) and the lower adsorption and extraction module (42) can be detachably arranged at the nucleic acid extraction position, the detection module (5) is detachably arranged at the nucleic acid detection position, the sample loading and removing module (6) is detachably arranged on the operation platform (1), and the sample loading and removing module (6) is used for transferring liquid between the sample loading position, the consumable position, the nucleic acid extraction position and the nucleic acid detection position.

2. The nucleic acid detecting apparatus according to claim 1, characterized in that the upper adsorption extraction module (41) and the lower adsorption extraction module (42) each have a first housing (401) and a first filter assembly (402), and the detection module (5) has a second housing (501) and a second filter assembly (502);

the exhaust port of the first filter assembly (402) of the upper or lower adsorption extraction module (41, 42) located at the nucleic acid extraction site and the exhaust port of the second filter assembly (502) of the detection module (5) located at the nucleic acid detection site are oriented in different directions, respectively.

3. The nucleic acid detecting apparatus according to claim 2, characterized in that the operating platform (1) has a first region (11), a second region (12) and a third region (13), the loading position and the consumable position are located in the first region (11), the nucleic acid extraction position is located in the second region (12), the nucleic acid detection position is located in the third region (13), and the second region (12) and the third region (13) are located on opposite or adjacent sides of the first region (11), respectively.

4. The nucleic acid detecting apparatus according to claim 2, wherein the first housing (401) of the upper adsorption extraction module (41) is detachably disposed on the operation platform (1), the upper adsorption extraction module (41) further comprises an upper magnetic attraction mechanism (411) and a first deep-well plate carrier (412) disposed in the first housing (401), the first deep-well plate carrier (412) carries a first deep-well plate (413), and the upper magnetic attraction mechanism (411) can adsorb, transfer and release magnetic beads in the first deep-well plate (413) from above.

5. The nucleic acid detecting apparatus according to claim 2, characterized in that the first housing (401) of the lower adsorption extraction module (42) is detachably disposed on the operation platform (1), the lower adsorption extraction module (42) further comprises a lower magnetic attraction mechanism (421) disposed in the first housing (401), a second deep well plate carrier (422), and a waste liquid removing mechanism (423), the second deep well plate carrier (422) carries thereon a second deep well plate (424), the lower magnetic attraction mechanism (421) is disposed below the second deep well plate carrier (422), a magnetic attraction member of the lower magnetic attraction mechanism (421) is movable toward or away from the second deep well plate (424) to adsorb magnetic beads in the second deep well plate (424) to a side wall or bottom of the second deep well plate (424), and to desorb magnetic beads in the second deep well plate (424), the removing mechanism (423) is used for transferring waste liquid in the second deep well plate (424).

6. The nucleic acid detecting apparatus according to claim 2, characterized in that the second housing (501) is detachably provided on the operation platform (1), the second filter assembly (502) is provided in plurality, and a plurality of the second filter assemblies (502) are all provided in the second housing (501), the detecting module (5) further comprises a plurality of PCR instruments (503) provided in the second housing (501), and a plurality of the PCR instruments (503) are provided in one-to-one correspondence with a plurality of the second filter assemblies (502).

7. The nucleic acid detecting apparatus according to claim 1, wherein the sample addition pipetting module (6) includes a mounting frame (61), a driving mechanism (62) provided on the mounting frame (61), and a pipetting mechanism (63) provided on the driving mechanism (62), the mounting frame (61) being detachably mountable on the operation platform (1), the pipetting mechanism (63) being movable back and forth between any one of the upper and lower suction extraction modules (41, 42), the sample addition module (2), the consumable module (3), and the detection module (5) under the action of the driving mechanism (62) to effect transfer of a liquid.

8. The nucleic acid detecting apparatus according to claim 1, wherein the loading module (2) includes a sample tube carrier (21) and a barcode scanner (22), the sample tube carrier (21) is used for loading sample tubes, the sample tube carrier (21) and the barcode scanner (22) are detachably provided on the operation platform (1), and the barcode scanner (22) scans the sample tubes on the sample tube rack one by one and records experimental information of each sample tube.

9. The nucleic acid detecting apparatus according to claim 1, characterized in that the consumable module (3) comprises:

the consumable bearing plate (31) is detachably arranged on the operation platform (1);

The PCR tube carrier (32) and the PCR tube, wherein the PCR tube carrier (32) is arranged on the consumable carrier plate (31), and the PCR tube is arranged on the PCR tube carrier (32);

a reagent tank carrier (33) and a reagent tube, wherein the reagent tank carrier (33) is arranged on the consumable carrier plate (31), and the reagent tube is arranged on the reagent tank carrier (33); and

The gun head carrier (34) and gun head, the gun head carrier (34) set up in on the consumptive material loading board (31), the gun head set up in on the gun head carrier (34).

10. The nucleic acid detecting apparatus according to any one of claims 1 to 9, wherein positioning rails are provided on the loading position, the consumable position, the nucleic acid extraction position, and the nucleic acid detecting position, respectively, the loading module (2), the consumable module (3), and the detecting module (5) are detachably mounted on the corresponding positioning rails, respectively, and any one of the upper adsorption extraction module (41) and the lower adsorption extraction module (42) is detachably mountable on the corresponding positioning rails.