CN220961355U - Non-transfer electrophoresis device capable of directly performing gel imaging - Google Patents
Non-transfer electrophoresis device capable of directly performing gel imaging Download PDFInfo
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- CN220961355U CN220961355U CN202322001595.XU CN202322001595U CN220961355U CN 220961355 U CN220961355 U CN 220961355U CN 202322001595 U CN202322001595 U CN 202322001595U CN 220961355 U CN220961355 U CN 220961355U
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 61
- 238000012546 transfer Methods 0.000 title claims abstract description 20
- 238000003384 imaging method Methods 0.000 title abstract description 13
- 239000011521 glass Substances 0.000 claims description 14
- 239000004983 Polymer Dispersed Liquid Crystal Substances 0.000 claims description 8
- 239000004793 Polystyrene Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 claims description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 3
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 15
- 239000000499 gel Substances 0.000 description 14
- 230000005611 electricity Effects 0.000 description 11
- 239000003792 electrolyte Substances 0.000 description 11
- 230000002572 peristaltic effect Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- 239000000377 silicon dioxide Substances 0.000 description 1
- HUAUNKAZQWMVFY-UHFFFAOYSA-M sodium;oxocalcium;hydroxide Chemical compound [OH-].[Na+].[Ca]=O HUAUNKAZQWMVFY-UHFFFAOYSA-M 0.000 description 1
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Abstract
The utility model discloses a non-transfer electrophoresis device capable of directly performing gel imaging, which relates to the technical field of gel imaging and comprises a box body, wherein a pipetting mechanism and an electrophoresis mechanism are arranged in the box body, the pipetting mechanism comprises a kit fixedly connected with the box body and two first electric push rods, the output ends of the two first electric push rods are fixedly connected with fixed blocks, one side surface of the fixed block positioned at the left side in the two fixed blocks is fixedly connected with a servo motor, the output shaft end of the servo motor is fixedly connected with a screw rod, and the two fixed blocks are both rotationally connected with the screw rod. According to the utility model, through the matching arrangement among the box body, the pipetting mechanism and the electrophoresis mechanism, the sample can be automatically spotted into the hole, then the electrophoresis is automatically carried out, the photo is automatically collected after the time is reached, the full-automatic operation is realized, the related error possibly caused by the manual operation is reduced, the time and the labor are saved, the working efficiency and the result accuracy of the whole operation process are improved, and the actual use requirement is met.
Description
Technical Field
The utility model relates to the technical field of gel imaging, in particular to a non-transfer electrophoresis device capable of directly performing gel imaging.
Background
Gel imaging, namely a laboratory instrument for cutting, photographing, observing and analyzing DNA or RNA gel, can be applied to bioengineering routine researches such as molecular weight calculation, density scanning, density quantification, PCR quantification and the like.
After the extraction of DNA molecules, the number and quality thereof need to be detected by electrophoresis techniques, and since agarose and polyacrylamide gels are introduced into nucleic acid studies, gel electrophoresis techniques for separating DNA according to the relative molecular mass size have been developed as an important experimental means for analyzing and identifying DNA molecules.
At present, the DNA molecules are required to be placed in an electrophoresis tank for electrophoresis separation and then photographed and analyzed after being extracted, but the existing steps of sample adding, electrophoresis, photographed and analyzed on the DNA molecules are all manually operated in a single step, so that the operation is complex, time and labor are consumed, related errors can be caused by human factors, the whole working efficiency is low, and the actual use requirements are not met; to this end, we provide a direct gel-imageable non-transfer electrophoresis apparatus that solves the above problems.
Disclosure of utility model
The utility model aims to make up the defects of the prior art, and provides a non-transfer electrophoresis device capable of directly performing gel imaging, which can automatically spot a sample into a hole, automatically perform electrophoresis, automatically collect a photo after the time is up, realize full-automatic operation, reduce related errors possibly caused by manual operation, and save time and labor.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a but non-transfer electrophoresis device of direct gel formation of image, comprises a box body, the inside of box is provided with pipetting mechanism and electrophoresis mechanism, pipetting mechanism includes kit and two first electric putter with box fixed connection, the equal fixedly connected with fixed block of output of two first electric putter, one side fixedly connected with servo motor of the fixed block that is located left among two fixed blocks, servo motor's output axle head fixedly connected with screw rod, two fixed blocks all rotate with the screw rod to be connected, the surface threaded connection of screw rod has the slider, slider and box sliding connection, the bottom surface fixedly connected with second electric putter of slider, the output fixedly connected with backup pad of second electric putter, a plurality of pipetting gun is installed to the bottom surface of backup pad, electrophoresis mechanism includes electrophoresis tank, the ultraviolet light source, first camera and the electrolyte pot with box fixed connection, integrated into one piece's ultraviolet-transmitting board is installed to electrophoresis tank's bottom.
Further, the front of box has the chamber door through the hinge, PDLC light modulation glass is installed to the inner wall of chamber door, can protect box inner structure to be convenient for observe the condition of gel formation of image when needs.
Further, the left side face and the right side face of the box body are provided with grooves, and the device can be conveniently moved to a proper position for use.
Further, the inner wall fixedly connected with power module of box, two electric putter all is connected with power module electricity, can store the electric energy to supply power for the electric component in the device when using.
Further, the servo motor is electrically connected with the power module, the second electric push rod is electrically connected with the power module, and the power module is connected with the servo motor and the second electric push rod through wires.
Further, the electrophoresis tank is electrically connected with the power supply module, the ultraviolet light source is electrically connected with the power supply module, and the power supply module is connected with the electrophoresis tank and the ultraviolet light source through wires.
Further, first camera and power module electricity are connected, two symmetrical second cameras are installed to the inside wall of box, can make things convenient for remote monitoring operation process to carry out real-time recording to operation process, so that follow-up looking over, two second cameras all are connected with power module electricity, and power module passes through the wire and is connected with first camera and second camera.
Further, the ultraviolet-transmitting plate arranged at the bottom of the electrophoresis tank can be made of optical quartz glass, ultraviolet-transmitting black glass, sodium-calcium-silicon-ultraviolet glass, acrylic PMMA and polystyrene PS, the electrophoresis tank is in an inverted concave shape, and the ultraviolet light source is positioned below the recess of the electrophoresis tank.
Compared with the prior art, the non-transfer electrophoresis device capable of direct gel imaging comprises the following components
The beneficial effects are that:
1. According to the utility model, through the matching arrangement among the box body, the pipetting mechanism and the electrophoresis mechanism, the sample can be automatically spotted into the hole, then the electrophoresis is automatically carried out, the photo is automatically collected after the time is reached, the full-automatic operation is realized, the related error possibly caused by the manual operation is reduced, the time and the labor are saved, the working efficiency and the result accuracy of the whole operation process are improved, and the actual use requirement is met.
2. According to the utility model, the box door and PDLC dimming glass are arranged, so that the internal structure of the box body can be protected, gel imaging conditions can be conveniently observed when needed, the whole device can be conveniently moved by arranging the grooves, the practicability of the device is improved, the remote monitoring operation process can be conveniently monitored by arranging the second camera, the operation process can be recorded in real time so as to be checked later, the power module is arranged, electric energy can be stored, and the electric elements in the device can be supplied with power when the device is used, the device is not required to be connected with an external power supply at any time, and the practicability of the device is improved.
Drawings
FIG. 1 is a schematic elevation view of a three-dimensional structure of the present utility model;
FIG. 2 is a front cross-sectional view of a three-dimensional structure of the present utility model;
FIG. 3 is a schematic view of a partial structure of the present utility model;
fig. 4 is an enlarged schematic view of the structure a in fig. 2 according to the present utility model.
In the figure: 1. a case; 2. a pipetting mechanism; 201. a first electric push rod; 202. a fixed block; 203. a servo motor; 204. a screw; 205. a slide block; 206. a second electric push rod; 207. a support plate; 208. a pipette gun; 209. a kit; 3. an electrophoresis mechanism; 301. an electrophoresis tank; 302. a uv-transparent plate; 303. an ultraviolet light source; 304. a first camera; 305. an electrolyte tank; 4. a door; 5. a groove; 6. a second camera; 7. and a power supply module.
Detailed Description
The principles and features of the present utility model are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the utility model and are not to be construed as limiting the scope of the utility model.
As described in the background art, the existing steps of sample adding, electrophoresis, photographing and analysis on DNA molecules are all manually operated in a single step, so that the operation is complex, time and labor are consumed, and related errors may be caused by human factors.
Referring to fig. 1 to 4, the present embodiment provides a non-transfer electrophoresis apparatus capable of direct gel imaging, which comprises a case 1, wherein a pipetting mechanism 2 and an electrophoresis mechanism 3 are disposed in the case 1, and the pipetting mechanism 2 comprises a kit 209 fixedly connected with the case 1 and two first electric push rods 201.
Be provided with touch screen control panel on the box 1, from taking corresponding master controller at touch screen control panel's inner wall, the actuating element in pipetting mechanism 2 and the electrophoresis mechanism 3 all is connected with this master controller electricity, and the operation of pipetting mechanism 2 and electrophoresis mechanism 3 can easily be realized as long as the operator operates this touch screen control panel through touching promptly, and easy operation is convenient.
The kit 209 is fixed in the inner wall of the case 1, and an operator mixes the reagent with the reagent to be detected in a test tube and places the mixed reagent in the kit 209 for subsequent detection.
The front of the box body 1 is hinged with a box door 4 through a hinge, PDLC dimming glass is installed on the inner wall of the box door 4, the inner structure of the box body 1 can be protected by arranging the box door 4 and the PDLC dimming glass, and the running condition of each part in the box body 1 can be conveniently observed at any time.
When the PDLC dimming glass is used for gel imaging, the power supply is turned off, and liquid crystal molecules in the PDLC dimming glass can be in an irregular dispersion state, so that light cannot enter, and the PDLC glass has an opaque appearance.
The output ends of the two first electric push rods 201 are fixedly connected with fixed blocks 202, one side surface of the fixed block 202 positioned on the left side of the two fixed blocks 202 is fixedly connected with a servo motor 203, the output shaft end of the servo motor 203 is fixedly connected with a screw 204, the two fixed blocks 202 are rotationally connected with the screw 204, the outer surface of the screw 204 is in threaded connection with a sliding block 205, and the sliding block 205 is in sliding connection with the box body 1.
The two first electric push rods 201 are synchronously started to respectively drive the two fixed blocks 202 to slide in the inner wall of the box body 1, the two fixed blocks 202 drive the servo motor 203 and the screw 204 to move, the servo motor 203 is started, the screw 204 can be driven to rotate forward and backward, and the screw 204 drives the sliding block 205 to slide left and right in the box body 1.
The bottom surface of the sliding block 205 is fixedly connected with a second electric push rod 206, the output end of the second electric push rod 206 is fixedly connected with a supporting plate 207, the bottom surface of the supporting plate 207 is provided with a plurality of pipetting guns 208,
The slide block 205 drives the second electric push rod 206 to move left and right, the second electric push rod 206 drives the pipetting gun 208 to move left and right through the supporting plate 207, the second electric push rod 206 is started, the pipetting gun 208 can be driven to move up and down through the supporting plate 207, and pipetting transfer is carried out on the mixed reagent in the reagent box 209.
The intervals among the pipette guns 208 are consistent with the intervals among the holes in the kit 209, and the pipette guns 208 are in one-to-one correspondence, so that excessive description is omitted, a plurality of equal-height liquid levels can be sucked simultaneously through the arrangement of the pipette guns 208, and the transfer efficiency of the reagent is improved.
Electrophoresis mechanism 3 includes electrophoresis tank 301, ultraviolet light source 303, first camera 304 and electrolyte jar 305 with box 1 fixed connection, and integrated into one piece's ultraviolet-transmitting board 302 is installed to electrophoresis tank 301's bottom.
The electrophoresis tank 301 is a place where electrochemical reaction or electrolysis occurs, and is composed of an anode and a cathode of two conductive electrodes, wherein the electrodes are connected by a circuit and made of ultraviolet light-transmitting materials.
A peristaltic pump is mounted on the electrolyte tank 305, and is activated before pipetting, so that electrolyte in the electrolyte tank 305 can be pumped into the electrophoresis tank 301 and the ultraviolet-transmitting plate 302 is covered.
The peristaltic pump is the prior art, comprises driver, pump head and peristaltic pump hose, is a controllable velocity of flow's liquid conveyor, and is not repeated here.
The interval between the pipette guns 208 is consistent with the interval between the holes on the ultraviolet-transmitting plate 302, and the reagent points sucked in the pipette guns 208 are in one-to-one correspondence to the corresponding holes on the ultraviolet-transmitting plate 302, sample addition is carried out under the liquid level, and then electrophoresis is carried out for 30-60 minutes.
After electrophoresis, a peristaltic pump is started to pump electrolyte out of the electrophoresis tank 301, then an ultraviolet light source 303 and a first camera 304 are started, a fluorescent photo is collected and subsequent analysis is carried out, and the first camera 304 is positioned right above the ultraviolet-transmitting plate 302.
The ultraviolet-transmitting plate 302 mounted at the bottom of the electrophoresis tank 301 may be made of optical quartz glass, ultraviolet-transmitting black glass, soda lime silica ultraviolet glass, acrylic PMMA, or polystyrene PS.
The electrophoresis tank 301 is in an inverted concave shape, and the ultraviolet light source 303 is positioned below the recess of the electrophoresis tank 301.
The left side face and the right side face of the box body 1 are provided with grooves 5, and the grooves 5 are arranged, so that the whole device can be conveniently moved, and the practicality of the device is improved.
Two symmetrical second cameras 6 are installed on the inner side wall of the box body 1, and the remote monitoring operation process can be conveniently and real-time recording is carried out on the operation process by arranging the second cameras 6 so as to facilitate subsequent checking.
The inner wall fixedly connected with power module 7 of box 1, two first electric putter 201 all are connected with power module 7 electricity, and servo motor 203 is connected with power module 7 electricity, and second electric putter 206 is connected with power module 7 electricity, and electrophoresis tank 301 is connected with power module 7 electricity, and ultraviolet light source 303 is connected with power module 7 electricity, and first camera 304 is connected with power module 7 electricity, and two second cameras 6 all are connected with power module 7 electricity.
Through setting up power module 7, can store the electric energy to supply power for the electrical component in the device when using, need not the device constantly to be connected with external power source, improve device practicality.
Working principle: when the direct gel imaging non-transfer electrophoresis device is used for gel electrophoresis, an operator mixes a reagent and a reagent to be detected in a test tube and then places the mixed reagent and the reagent in a reagent box 209, a touch screen control panel on a box body 1 is used for controlling the operation of a pipetting mechanism 2 and an electrophoresis mechanism 3, a peristaltic pump on an electrolyte tank 305 is controlled to be started, electrolyte in the electrolyte tank 305 is pumped into an electrophoresis tank 301 and covers an ultraviolet-transmitting plate 302, then two first electric push rods 201 are controlled to be synchronously started, the two first electric push rods 201 respectively drive two fixed blocks 202 to slide in the inner wall of the box body 1, the two fixed blocks 202 drive a servo motor 203 and a screw 204 to move, the servo motor 203 is started, the screw 204 can be driven to rotate, the screw 204 drives a slide block 205 to slide in the box body 1, the slide block 205 drives a second electric push rod 206 to move, the second electric push rod 206 drives a pipetting gun 208 to move through a support plate 207, starting a second electric push rod 206, driving a liquid-transferring gun 208 to move up and down through a supporting plate 207, transferring the mixed reagent in a reagent box 209, moving the liquid-transferring gun 208 to the position above an ultraviolet-transmitting plate 302, controlling the liquid-transferring gun 208 to point the sucked reagent into a corresponding hole on the ultraviolet-transmitting plate 302, loading samples under the liquid level, then carrying out electrophoresis for 30-60 minutes, starting a peristaltic pump to extract electrolyte out of an electrophoresis tank 301 after the electrophoresis is finished, then controlling an ultraviolet light source 303 and a first camera 304 to start, collecting fluorescent photos and carrying out subsequent analysis, the utility model can realize automatic point-to-hole and automatic electrophoresis, automatically collect photos after time, realize full-automatic operation, reduce related errors possibly caused by manual operation, save time and labor, improve the working efficiency and result accuracy of the whole operation process, meets the practical use requirement.
Claims (8)
1. A direct gel-imageable non-transfer electrophoresis apparatus comprising a housing (1), characterized in that: the inside of box (1) is provided with pipetting mechanism (2) and electrophoresis mechanism (3), pipetting mechanism (2) include with box (1) fixed connection's kit (209) and two first electric putter (201), the equal fixedly connected with fixed block (202) of output of two first electric putter (201), be located one side fixedly connected with servo motor (203) of left fixed block (202) in two fixed blocks (202), the output axle head fixedly connected with screw rod (204) of servo motor (203), two fixed blocks (202) all rotate with screw rod (204) to be connected, the surface threaded connection of screw rod (204) has slider (205), slider (205) and box (1) sliding connection, the bottom surface fixedly connected with second electric putter (206) of slider (205), the bottom surface of backup pad (207) installs a plurality of liquid-transfering gun (208), electrophoresis mechanism (3) include with box (1) fixed connection's groove (301), electrophoresis tank (305), electrophoresis of first ultraviolet light source (305) and electrophoresis molding of integrative shell-side plate (302).
2. A direct gel imageable non-transfer electrophoretic device as claimed in claim 1, wherein: the front of the box body (1) is hinged with a box door (4) through a hinge, and PDLC dimming glass is installed on the inner wall of the box door (4).
3. A direct gel imageable non-transfer electrophoretic device as claimed in claim 1, wherein: grooves (5) are formed in the left side face and the right side face of the box body (1).
4. A direct gel imageable non-transfer electrophoretic device as claimed in claim 1, wherein: the inner wall of the box body (1) is fixedly connected with a power module (7), and the two first electric push rods (201) are electrically connected with the power module (7).
5. A direct gel imageable non-transfer electrophoretic device as claimed in claim 4 wherein: the servo motor (203) is electrically connected with the power module (7), and the second electric push rod (206) is electrically connected with the power module (7).
6. A direct gel imageable non-transfer electrophoretic device as claimed in claim 4 wherein: the electrophoresis tank (301) is electrically connected with the power module (7), and the ultraviolet light source (303) is electrically connected with the power module (7).
7. A direct gel imageable non-transfer electrophoretic device as claimed in claim 4 wherein: the first camera (304) is electrically connected with the power module (7), two symmetrical second cameras (6) are mounted on the inner side wall of the box body (1), and the two second cameras (6) are electrically connected with the power module (7).
8. A direct gel imageable non-transfer electrophoretic device as claimed in claim 1, wherein: the ultraviolet-transmitting plate (302) arranged at the bottom of the electrophoresis tank (301) can be made of optical quartz glass, ultraviolet-transmitting black glass, sodium-calcium-silicon-ultraviolet glass, acrylic PMMA and polystyrene PS.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322001595.XU CN220961355U (en) | 2023-07-28 | 2023-07-28 | Non-transfer electrophoresis device capable of directly performing gel imaging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322001595.XU CN220961355U (en) | 2023-07-28 | 2023-07-28 | Non-transfer electrophoresis device capable of directly performing gel imaging |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220961355U true CN220961355U (en) | 2024-05-14 |
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ID=91010191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322001595.XU Active CN220961355U (en) | 2023-07-28 | 2023-07-28 | Non-transfer electrophoresis device capable of directly performing gel imaging |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220961355U (en) |
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2023
- 2023-07-28 CN CN202322001595.XU patent/CN220961355U/en active Active
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