CN213714893U - Slide pretreatment system - Google Patents
Slide pretreatment system Download PDFInfo
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- CN213714893U CN213714893U CN202022735294.6U CN202022735294U CN213714893U CN 213714893 U CN213714893 U CN 213714893U CN 202022735294 U CN202022735294 U CN 202022735294U CN 213714893 U CN213714893 U CN 213714893U
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Abstract
The utility model discloses a slide pretreatment systems, the load simulator comprises a system body, the system body includes that bottom plate, cover are located the shell subassembly on bottom plate top, set up power supply module, master control drive assembly, solenoid valve drive assembly and constant temperature bath subassembly between shell subassembly and bottom plate, master control drive assembly is including being fixed in liquid feeding peristaltic pump and the flowing back peristaltic pump on the bottom plate, solenoid valve subassembly includes that reagent input channel switches solenoid valve, waste liquid evacuation passageway switch solenoid valve and reagent distribution passageway switch solenoid valve, constant temperature bath subassembly including be fixed in the mount at the relative both ends in bottom plate top, rotate through the pivot and connect the sample platform between two mounts and be fixed in the sample bench sample constant temperature spare, be equipped with between sample platform bottom and the bottom plate and be used for driving sample platform pivoted rocker mechanism. The utility model has the advantages of reasonable design, convenient to use can effectual reduction test result error, is favorable to the popularization and application of marketization.
Description
Technical Field
The utility model relates to the field of biotechnology, concretely relates to slide pretreatment systems.
Background
In situ hybridization refers to the process of hybridizing specifically labeled nucleic acids of known sequence as probes to nucleic acids in cells or tissue sections to precisely quantify the specific nucleic acid sequence. The specific principle is as follows: according to the nucleic acid base complementary pairing principle, a hapten labeled DNA or RNA probe is complementary paired with a denatured single-chain nucleic acid sequence, the hapten is identified through an antibody with a fluorescent group for detection, or the probe is directly labeled by the fluorescent group and combined with a target sequence, and finally the distribution condition of the target sequence in a cell nucleus, a chromosome or a slice tissue is directly observed by using a fluorescence microscope. The technology is widely applied to the fields of animal and plant genome structure research, chromosome fine structure variation analysis, virus infection analysis, human prenatal diagnosis, tumor genetics and genome evolution research.
However, in situ hybridization instruments conventionally used in the market at present need a large number of pretreatment steps performed by technicians before application, which not only wastes a large amount of time and increases the workload of operators, but also causes great errors in the final test result due to the addition of a large number of human factors, and thus cannot meet the requirements of people.
SUMMERY OF THE UTILITY MODEL
The utility model provides a solve above-mentioned problem, provide a structural design is reasonable, and convenient to use is swift, slide pretreatment systems that can effectual reduction test result error.
The utility model adopts the following technical scheme:
a slide pretreatment system comprises a system body, wherein the system body comprises a bottom plate, a shell component covered on the top end of the bottom plate, a power supply component, a master control driving component, an electromagnetic valve driving component and a thermostatic bath component, the power supply component is arranged between the shell component and the bottom plate, the power supply component is fixed on one side edge of the top end of the bottom plate, the master control driving component comprises a liquid feeding peristaltic pump and a liquid discharging peristaltic pump which are fixed on one side edge of the bottom plate opposite to the power supply component, the electromagnetic valve component comprises a reagent input channel switching electromagnetic valve, a waste liquid emptying channel switching electromagnetic valve and a reagent distribution channel switching electromagnetic valve, the reagent input channel switching electromagnetic valve is respectively fixed at two opposite ends of the top of the bottom plate, the output end of the reagent input channel switching electromagnetic valve is communicated with the input end of the liquid feeding peristaltic pump, the waste liquid evacuation passageway switches input and the output intercommunication of flowing back peristaltic pump of solenoid valve, the constant temperature cell subassembly is including the mount that is fixed in the relative both ends in bottom plate top, rotate the sample platform of connecting between two mounts and a plurality of group are fixed in the sample bench sample constant temperature spare, be equipped with between sample platform bottom and the bottom plate and be used for driving sample platform pivoted rocker mechanism.
As an optimal technical scheme of the utility model, shell subassembly top end tip is equipped with the opening, the opening upper cover has closed the glass upper cover, a side of glass upper cover is articulated each other with the corresponding position department of shell subassembly, the other end of glass upper cover is the expansion end.
As an optimal technical scheme of the utility model, the top one end of bottom plate is equipped with a fixed plate, fixed USB interface and VGR video interface of being equipped with on the fixed plate.
As a preferred technical scheme of the utility model, master control drive assembly still including be fixed in power supply module one side and respectively with power supply module electric connection's master control drive circuit board and supply socket, liquid feeding peristaltic pump and flowing back peristaltic pump pass through the wire with master control drive circuit board respectively and are connected.
As a preferred technical scheme of the utility model, solenoid valve drive assembly still includes solenoid valve drive circuit board, the bottom plate top is equipped with the cover and locates the outside outrigger of liquid feeding peristaltic pump and flowing back peristaltic pump, solenoid valve drive circuit board is fixed in on the outrigger, reagent input channel switches solenoid valve, waste liquid evacuation passageway switches solenoid valve and reagent distribution passageway switches solenoid valve respectively with solenoid valve drive circuit board signal connection, solenoid valve drive circuit board still with master control drive circuit board signal connection.
As a preferred technical scheme of the utility model, the constant temperature groove subassembly still includes that a plurality of is fixed in the sample platform bottom and with the constant temperature groove circuit control board that the sample constant temperature piece one-to-one set up, a plurality of constant temperature groove circuit control board respectively with master control drive circuit board signal connection.
As a preferred technical scheme of the utility model, the top of the sample constant temperature part is provided with a plurality of constant temperature tanks, the top of the sample constant temperature part is also provided with a sealing cover which covers the constant temperature tanks, the side wall of the sample constant temperature part is provided with a reagent channel component which is arranged corresponding to the constant temperature tanks one by one and is communicated with the inside of the constant temperature tanks, the reagent channel component comprises a liquid inlet joint and a liquid outlet joint, the liquid inlet joint is communicated with the output end of a reagent distribution channel switching electromagnetic valve, the liquid outlet joint is communicated with the input end of a liquid discharge peristaltic pump, the side wall of the sample constant temperature part is also provided with a temperature sensor for detecting the temperature inside the constant temperature tanks, the outer side of the bottom wall of the sample constant temperature part is fixedly provided with a heating film and a heating film PCB plate, the bottom end of the heating film is fixedly provided with a backing plate, and the heating film PCB and the temperature sensor are respectively in signal connection with the thermostatic bath circuit control board.
As a preferred technical scheme of the utility model, rocker mechanism includes lead screw step motor, connecting rod and is the link of "T" font, the fixed bearing that is equipped with down on lead screw step motor's the output, the bottom of connecting rod articulates on lower bearing, the bottom of sample platform is fixed in on the top of link, the bottom mounting of link is equipped with the upper bearing, the top of connecting rod articulates on the upper bearing.
The utility model has the advantages that:
the utility model has reasonable structural design, simple and convenient operation and high automation degree, can effectively avoid accidental errors brought to the test result by excessive manual operation, and improves the test accuracy; the rocker mechanism that sets up simultaneously can great improvement hybridization reaction effect, improves the result of use of system's body.
Drawings
FIG. 1 is a schematic view of the internal three-dimensional structure of the system body of the present invention;
FIG. 2 is a front view structural diagram of FIG. 1;
FIG. 3 is a rear view structural diagram of FIG. 1;
FIG. 4 is a side view structural diagram of FIG. 1;
FIG. 5 is a schematic cross-sectional view taken at D-D in FIG. 2;
FIG. 6 is a schematic structural view of the housing assembly of the present invention;
fig. 7 is a schematic structural view between the bottom plate and the main control driving assembly of the present invention;
fig. 8 is a schematic structural view between the bottom plate and the solenoid valve driving assembly of the present invention;
FIG. 9 is a schematic perspective view of a sample thermostatic element according to the present invention;
FIG. 10 is a front view structural diagram of FIG. 9;
FIG. 11 is a side view of the structure of FIG. 9;
the symbols in the drawings illustrate that:
the device comprises a bottom plate 1, a shell component 2, a power supply component 3, a liquid feeding peristaltic pump 4, a liquid discharging peristaltic pump 5, a reagent input channel switching electromagnetic valve 6, a waste liquid emptying channel switching electromagnetic valve 7, a reagent distribution channel switching electromagnetic valve 8, a fixing frame 9, a sample table 10, a sample constant temperature component 11, a glass upper cover 12, a fixing plate 13, a USB interface 14, a VGR video interface 15, a main control driving circuit board 16, a power socket 17, an electromagnetic valve driving circuit board 18, an outer frame 19, a constant temperature bath circuit control board 20, a constant temperature bath 21, a sealing cover 22, a reagent channel component 23, a temperature sensor 24, a heating film 25, a heating film PCB board 26, a refrigerating sheet 27, a cooling fin mounting plate 28, a cooling fin 29, a lead screw stepping motor 30, a connecting rod 31, a connecting frame 32.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings.
As shown in fig. 1 to 11, a slide pretreatment system includes a system body including a base plate 1, a housing assembly 2 covering a top end of the base plate 1, a power supply assembly 3 disposed between the housing assembly 2 and the base plate 1, a main control drive assembly, a solenoid valve drive assembly, and a thermostatic bath assembly, the power supply component 3 is fixed on one side edge of the top end of the bottom plate 1, the main control driving component comprises a liquid feeding peristaltic pump 4 and a liquid discharging peristaltic pump 5 which are fixed on one side edge of the bottom plate 1 opposite to the power supply component 3, during preparation, reserved openings matched with the liquid feeding peristaltic pump 4 and the liquid discharging peristaltic pump 5 are formed in the shell component 2 at positions corresponding to the liquid feeding peristaltic pump 4 and the liquid discharging peristaltic pump 5, and the liquid feeding peristaltic pump 4 and the liquid discharging peristaltic pump 5 can be clamped with the shell component 2 conveniently during assembly;
the electromagnetic valve component comprises a reagent input channel switching electromagnetic valve 6, a waste liquid emptying channel switching electromagnetic valve 7 and a reagent distribution channel switching electromagnetic valve 8, wherein the reagent input channel switching electromagnetic valve 6 and the waste liquid emptying channel switching electromagnetic valve 7 are respectively fixed at two opposite ends of the top of the bottom plate 1, the reagent distribution channel switching electromagnetic valve 8 is fixed above the power supply component 3 through a right-angle fixing plate, the output end of the reagent input channel switching electromagnetic valve 6 is communicated with the input end of a liquid adding peristaltic pump 4, the output end of the liquid adding peristaltic pump 4 is communicated with the input end of the reagent distribution channel switching electromagnetic valve 8, the input end of the waste liquid emptying channel switching electromagnetic valve 7 is communicated with the output end of the liquid discharging peristaltic pump 5, when the electromagnetic valve component is used, an external reagent is communicated with the input; more specifically, in this embodiment, during preparation, reserved ports convenient for connection with external components are provided on the housing assembly 2 at positions corresponding to the input end of the reagent input channel switching solenoid valve 6 and the output end of the waste liquid emptying channel switching solenoid valve 7;
the thermostatic bath assembly comprises fixing frames 9 fixed at two opposite ends of the top of the bottom plate 1, a sample table 10 rotatably connected between the two fixing frames 9 through a rotating shaft, and a plurality of groups of sample thermostatic parts 11 fixed on the sample table 10, specifically, in the embodiment, the number of the sample thermostatic parts is 3, and in a specific application process, a person skilled in the art can determine the specific number of the sample thermostatic parts according to actual needs; e.g., two, four, five, etc.; further, a rocker mechanism for driving the sample table 10 to rotate relative to the fixing frame 9 is arranged between the bottom end of the sample table 10 and the bottom plate 1.
More specifically, an opening (not marked in the figure) is formed in the end portion of the top end of the housing assembly 2, a glass upper cover 12 is covered on the opening, one side edge of the glass upper cover 12 is hinged to a corresponding position of the housing assembly 2, and the other end of the glass upper cover 12 is a movable end;
more specifically, one end of the top of the bottom plate 1 is provided with a fixing plate 13, and the fixing plate 13 is fixedly provided with two USB interfaces 14 and a VGR video interface 15; the VGR video interface 15 is used for connecting with an external PC end;
further specifically, the main control driving assembly further comprises a main control driving circuit board 16 and a power socket 17 which are fixed on one side of the power supply assembly 3 and are electrically connected with the power supply assembly 3 respectively, the liquid feeding peristaltic pump 4 and the liquid discharging peristaltic pump 5 are connected with the main control driving circuit board 16 through leads respectively, and the USB interface 14 and the VGR video interface 15 are also connected with the main control driving circuit board 16 through leads respectively;
further specifically, the electromagnetic valve driving assembly further comprises an electromagnetic valve driving circuit board 18, an outer frame 19 covering the liquid adding peristaltic pump 4 and the liquid discharging peristaltic pump 5 is arranged at the top end of the bottom plate 1, the electromagnetic valve driving circuit board 18 is fixed on the outer frame 19, the input channel switching electromagnetic valve 6, the waste liquid emptying channel switching electromagnetic valve 7 and the reagent distribution channel switching electromagnetic valve 8 are respectively in signal connection with the electromagnetic valve driving circuit board 18, and the electromagnetic valve driving circuit board 18 is further in signal connection with a main control driving circuit board 16;
more specifically, the thermostatic bath assembly further includes a plurality of thermostatic bath circuit control boards 20 fixed at the bottom end of the sample stage 10 and arranged in one-to-one correspondence with the sample thermostatic parts 11, specifically, in this embodiment, the number of the thermostatic bath circuit control boards 20 is also three; the three constant temperature bath circuit control boards 20 are respectively in signal connection with the main control drive circuit board 16;
further specifically, the top end of the sample constant temperature part 11 is provided with a plurality of constant temperature grooves 21, specifically, in this embodiment, the number of the constant temperature grooves 21 in each sample constant temperature part 11 is two, the top end of the sample constant temperature part 11 is further provided with a sealing cover 22 for covering the constant temperature grooves 21, the side wall of the sample constant temperature part 11 is provided with a reagent passage component 23 which is arranged corresponding to the constant temperature grooves 21 one by one and communicated with the inside of the constant temperature grooves 21, the reagent passage component 23 comprises a liquid inlet joint and a liquid outlet joint, the liquid inlet joint is communicated with the output end of the reagent distribution passage switching electromagnetic valve 8, the liquid outlet joint is communicated with the input end of the liquid discharge peristaltic pump 5, the side wall of the sample constant temperature part 11 is further provided with a temperature sensor 24 for detecting the temperature inside the constant temperature grooves 21, and the outer side of the bottom wall of the sample constant temperature part 11 is fixedly provided with a, a backing plate (not marked in the figure), a refrigerating sheet 27, a cooling fin mounting plate 28 and a cooling fin 29 are fixedly arranged at the bottom end of the heating film 25 from top to bottom in sequence, and the heating film PCB 26 and the temperature sensor 24 are respectively in signal connection with the thermostatic bath circuit control board 20;
more specifically, the rocker mechanism comprises a screw rod stepping motor 30, a connecting rod 31 and a T-shaped connecting frame 32, a lower bearing 33 is fixedly arranged on the output end of the screw rod stepping motor 30, the bottom end of the connecting rod 31 is hinged to the lower bearing 33, the top end of the connecting frame 32 is fixed to the bottom end of the sample table 10, an upper bearing 34 is fixedly arranged at the bottom end of the connecting frame 32, and the top end of the connecting rod 31 is hinged to the upper bearing 34.
The utility model discloses the use profile is as follows:
when the device is used, firstly, a slide to be tested is placed on a slide rack, then the whole slide rack is placed in a constant temperature groove 21 and covered with a sealing cover 22, corresponding channels on a reagent input channel switching electromagnetic valve 6 and a reagent distribution channel switching electromagnetic valve 8 are controlled to be opened by an electromagnetic valve driving circuit board 18, and then a liquid adding peristaltic pump 4 is started by a main control driving circuit board 16 to convey the corresponding reagent to the corresponding constant temperature groove 21 with the slide rack; then, the heating film 25 is started through the thermostatic bath circuit control board 20 to heat the corresponding thermostatic bath 21; and finally, starting the screw rod stepping motor 30 to drive the connecting rod 31 to reciprocate up and down as required so as to drive the sample table 10 and the sample constant-temperature part on the sample table 10 to shake back and forth, so that the reaction effect is improved.
And after the reaction is finished, discharging the waste liquid in the corresponding constant temperature groove 21 through the waste liquid emptying channel switching electromagnetic valve 7 and the liquid discharge peristaltic pump 5.
Finally, it should be noted that: these embodiments are merely illustrative of the present invention and do not limit the scope of the invention. In addition, other variations and modifications will be apparent to persons skilled in the art based on the foregoing description. And are neither required nor exhaustive of all embodiments. And obvious changes and modifications may be made without departing from the scope of the present invention.
Claims (8)
1. A slide pretreatment system comprising a system body, characterized in that: the system body comprises a bottom plate (1), an outer shell component (2) covering the top end of the bottom plate (1), a power supply component (3) arranged between the outer shell component (2) and the bottom plate (1), a master control driving component, an electromagnetic valve driving component and a thermostatic bath component, wherein the power supply component (3) is fixed on one side edge of the top end of the bottom plate (1), the master control driving component comprises a liquid feeding peristaltic pump (4) and a liquid discharging peristaltic pump (5) which are fixed on the side edge of the bottom plate (1) opposite to the power supply component (3), the electromagnetic valve component comprises a reagent input channel switching electromagnetic valve (6) and a waste liquid discharging channel switching electromagnetic valve (7) which are respectively fixed on two opposite ends of the top of the bottom plate (1) and a reagent distribution channel switching electromagnetic valve (8) which is fixed above the power supply component (3), the output end of the reagent input channel switching electromagnetic valve (6) is, the output of liquid feeding peristaltic pump (4) and the input intercommunication of reagent distribution passageway switching solenoid valve (8), the input of waste liquid evacuation passageway switching solenoid valve (7) and the output intercommunication of flowing back peristaltic pump (5), the constant temperature cell subassembly is including being fixed in mount (9) at the relative both ends in bottom plate (1) top, rotate sample platform (10) and the sample constant temperature spare (11) that a plurality of groups are fixed in on sample platform (10) of connecting between two mount (9) through the pivot, be equipped with rocker mechanism between sample platform (10) bottom and bottom plate (1).
2. A slide pre-treatment system as claimed in claim 1, wherein: the utility model discloses a glass shell subassembly, including shell subassembly (2), opening upper cover (12), the glass upper cover is articulated each other with the corresponding position department of shell subassembly (2) in a side of glass upper cover (12), the other end of glass upper cover (12) is the expansion end.
3. A slide pre-treatment system as claimed in claim 1, wherein: the top one end of bottom plate (1) is equipped with a fixed plate (13), fixed USB interface (14) and VGR video interface (15) of being equipped with on the fixed plate.
4. A slide pre-treatment system as claimed in claim 1, wherein: the main control driving assembly further comprises a main control driving circuit board (16) and a power socket (17), wherein the main control driving circuit board (16) and the power socket are fixed on one side of the power supply assembly (3) and are respectively electrically connected with the power supply assembly (3), and the liquid feeding peristaltic pump (4) and the liquid discharging peristaltic pump (5) are respectively connected with the main control driving circuit board (16) through leads.
5. A slide pre-treatment system as claimed in claim 4, wherein: solenoid valve drive assembly still includes solenoid valve dirver circuit board (18), bottom plate (1) top is equipped with cover and locates liquid feeding peristaltic pump (4) and flowing back peristaltic pump (5) outside outrigger (19), solenoid valve dirver circuit board (18) are fixed in on outrigger (19), reagent input channel switches solenoid valve (6), waste liquid evacuation passageway switches solenoid valve (7) and reagent distribution passageway switches solenoid valve (8) respectively with solenoid valve dirver circuit board (18) signal connection, solenoid valve dirver circuit board (18) still with master control dirver circuit board (16) signal connection.
6. A slide pre-treatment system as claimed in claim 4, wherein: the constant temperature bath component further comprises a plurality of constant temperature bath circuit control boards (20) which are fixed at the bottom end of the sample table (10) and are in one-to-one correspondence with the sample constant temperature parts (11), and the constant temperature bath circuit control boards (20) are respectively in signal connection with the main control driving circuit board (16).
7. A slide pre-treatment system as claimed in claim 6, wherein: the top of sample constant temperature spare (11) is equipped with a plurality of constant temperature tank (21), the top of sample constant temperature spare (11) still is equipped with sealed lid (22) that closes constant temperature tank (21) lid, be equipped with on the lateral wall of sample constant temperature spare (11) and set up and with constant temperature tank (21) inside communicating reagent passageway subassembly (23) with constant temperature tank (21) one-to-one, reagent passageway subassembly (23) include liquid inlet joint and go out liquid joint, liquid inlet joint and the output intercommunication of reagent distribution passageway switching solenoid valve (8), go out the input intercommunication of liquid joint and flowing back peristaltic pump (5), still be equipped with temperature sensor (24) on the lateral wall of sample constant temperature spare (11), the diapire outside of sample constant temperature spare (11) is fixed to be equipped with heating membrane (25) and heating membrane PCB board (26), the bottom from the top down of heating membrane (25) is fixed in proper order and is equipped with the backing plate, The refrigerator comprises refrigeration fins (27), a cooling fin mounting plate (28) and cooling fins (29), wherein the heating film PCB (26) and the temperature sensor (24) are in signal connection with a thermostatic bath circuit control plate (20) respectively.
8. A slide pre-treatment system as claimed in claim 1, wherein: the rocker mechanism comprises a screw rod stepping motor (30), a connecting rod (31) and a T-shaped connecting frame (32), a lower bearing (33) is fixedly arranged at the output end of the screw rod stepping motor (30), the bottom end of the connecting rod (31) is hinged to the lower bearing (33), the top end of the connecting frame (32) is fixed to the bottom end of the sample table (10), an upper bearing (34) is fixedly arranged at the bottom end of the connecting frame (32), and the top end of the connecting rod (31) is hinged to the upper bearing (34).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022735294.6U CN213714893U (en) | 2020-11-23 | 2020-11-23 | Slide pretreatment system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022735294.6U CN213714893U (en) | 2020-11-23 | 2020-11-23 | Slide pretreatment system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213714893U true CN213714893U (en) | 2021-07-16 |
Family
ID=76783682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022735294.6U Active CN213714893U (en) | 2020-11-23 | 2020-11-23 | Slide pretreatment system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213714893U (en) |
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2020
- 2020-11-23 CN CN202022735294.6U patent/CN213714893U/en active Active
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