CN220887529U - Split type multichannel reagent card and nucleic acid detection device - Google Patents
Split type multichannel reagent card and nucleic acid detection device Download PDFInfo
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- CN220887529U CN220887529U CN202322606131.1U CN202322606131U CN220887529U CN 220887529 U CN220887529 U CN 220887529U CN 202322606131 U CN202322606131 U CN 202322606131U CN 220887529 U CN220887529 U CN 220887529U
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- reagent card
- component
- pressing plate
- inclined plane
- test strip
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 96
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 12
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 12
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 12
- 238000001514 detection method Methods 0.000 title abstract description 12
- 238000012360 testing method Methods 0.000 claims abstract description 39
- 238000003825 pressing Methods 0.000 claims abstract description 32
- 238000010438 heat treatment Methods 0.000 claims description 32
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 7
- 229910052744 lithium Inorganic materials 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 2
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 238000007689 inspection Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract 1
- 238000013461 design Methods 0.000 description 9
- 238000010276 construction Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003805 vibration mixing Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The application discloses a split multichannel reagent card and a nucleic acid detection device; belongs to the field of medical examination and inspection instruments and molecular diagnosis and detection instruments; the technical key points are as follows: the n sample adding holes correspond to n reaction bins, the inclined plane pressing plate assembly corresponds to the inclined plane channel, and 2 parallel inclined plane pressing plates of the inclined plane pressing plate assembly are arranged on the inner side of a side wall plate of the inclined plane channel; the second pressing plate assembly corresponds to the test paper supporting member; the lower surface of the test strip is arranged on the inclined surface channel and the test strip supporting member, and the upper surface of the test strip is pressed by the inclined surface pressing plate and the second pressing plate assembly; the test strip will display the results in the strip window. The application aims to provide a split type multichannel reagent card and a nucleic acid detection device, which can improve the detection effect of the detection card.
Description
Technical Field
The application relates to the fields of medical examination and inspection instruments and molecular diagnosis and detection instruments, in particular to a split multichannel reagent card and a nucleic acid detection device.
Background
The applicant provides a multichannel split type kit in CN115141745A, and the design key points of the kit are as follows: each cap channel includes: the inner wall of the first flow pipeline, the reaction bin diversion trench, the freeze-drying ball limiting rod and the capillary diversion trench; the sample adding port is sequentially communicated with the inner wall of the main liquid inlet bin, the secondary liquid inlet bin and the first flow pipeline in sequence; the lengths of the inner walls of the first flow pipelines of each top cover channel are the same, namely, the reaction bin diversion trenches of each top cover channel are distributed by taking the center of the sample adding port as the center of a circle and the lengths of the inner walls of the first flow pipelines as the radius from the view of the bottom.
For CN115141745A, it adopts the concept of split design. Which flows from 1 loading port to a plurality of first flow channels. However, products based on the concept of split design are more complex to design and manufacture.
Accordingly, development teams continue to develop in an effort to advance the design of CN 115141745A.
Disclosure of utility model
The application aims to overcome the defects of the prior art and provide a split multichannel reagent card.
Another object of the present application is to provide a nucleic acid detecting apparatus.
The technical scheme of the application is as follows:
A split multichannel reagent card comprising: a reagent card upper member, a reagent card lower member, n test strips;
The upper surface of the upper component of the reagent card is provided with a sample adding hole component, a window component and a window film in a protruding mode, the sample adding hole component is provided with n sample adding holes which are respectively communicated up and down, the window component is provided with n strip-shaped window grooves, and the upper surface of the window component is provided with the window film in a sealing mode; n is a natural number of 2 or more; the lower surface of the upper component of the reagent card is also provided with an inclined surface pressing plate component and a second pressing plate component;
Wherein the reagent card lower member comprises: the n reagent channels, each reagent channel includes in proper order according to the direction of solution flow: the reaction bin, the first horizontal bin, the inclined plane channel and the test paper supporting member; the bottom surface of the first horizontal bin is lower than the bottom surface of the reaction bin, and the height of the inclined plane channel is gradually increased along the advancing direction of the solution;
wherein, the cooperation relation of reagent card upper portion component, reagent card lower part component, test strip is:
The sample adding holes a and n correspond to the reaction bins n, the inclined plane pressing plate assembly corresponds to the inclined plane channel, and 2 parallel inclined plane pressing plates of the inclined plane pressing plate assembly are arranged on the inner side of a side wall plate of the inclined plane channel;
b, the second pressing plate assembly corresponds to the test paper supporting member;
c, the lower surface of the test strip is placed on the inclined surface channel and the test strip supporting member, and the upper surface of the test strip is pressed by the inclined surface pressing plate and the second pressing plate assembly;
and d, displaying a result on the strip-shaped window groove by the test strip.
Further, the well assembly protrudes from the upper and lower surfaces of the reagent card upper member.
Further, an opening is provided in the reagent card lower member, and a filter cartridge is provided in the opening.
Further, the device also comprises a cap; the cap is used for closing the upper surfaces of n loading holes of the loading hole assembly.
A nucleic acid detecting apparatus employing the split-type multichannel reagent card and the reagent card incubator;
Wherein, reagent card incubator includes: an upper shell and a lower cover of the reagent card incubator;
Wherein, place in the interior space that reagent card incubator upper casing and reagent card incubator lower cover body formed: the lithium battery, the vibration motor, the temperature control circuit board and the heating seat component;
Wherein, the heating seat component and the vibration motor are arranged on the temperature control circuit board;
The upper shell of the reagent card incubator is provided with a first mounting hole and a second bar-shaped groove;
wherein, the heating seat subassembly includes: the heating device comprises n heating seats and a spring guide mechanism, wherein the n heating seats are connected into a whole, one end of the spring guide mechanism is connected with the heating seats, and the other end of the spring guide mechanism is connected with a temperature control circuit board;
Wherein the vibration motor is adapted to the first mounting hole;
The lithium battery supplies power to the temperature control circuit board, the heating seat and the vibration motor;
the temperature control circuit board is used for controlling whether the heating seat and the vibration motor work or not;
Wherein, the n reaction bins of the upper component of the reagent card correspond to the n heating seats of the incubator of the reagent card, and the temperature of the reaction bins is kept at a proper temperature by the heating seats.
The application has the beneficial effects that:
Firstly, the multi-channel reagent card abandons the diversion technology proposed by CN115141745A and adopts the design idea of an independent channel; meanwhile, the design is simplified for the runner.
Specifically, the relationship between the upper member of the reagent card, the lower member of the reagent card, and the test strip is expressed.
The sample adding holes a and n correspond to the reaction bins n, the inclined plane pressing plate assembly corresponds to the inclined plane channel, and 2 parallel inclined plane pressing plates of the inclined plane pressing plate assembly are arranged on the inner side of a side wall plate of the inclined plane channel;
b, the second pressing plate assembly corresponds to the test paper supporting member;
c, the lower surface of the test strip is placed on the inclined surface channel and the test strip supporting member, and the upper surface of the test strip is pressed by the inclined surface pressing plate and the second pressing plate assembly;
and d, displaying a result on the strip-shaped window groove by the test strip.
Second, split type multichannel reagent card incubator can reuse, and split type multichannel reagent card and reagent card incubator are quick detach formula buckle, make things convenient for the dismouting.
Drawings
The application is described in further detail below in connection with the embodiments in the drawings, but is not to be construed as limiting the application in any way.
FIG. 1 is a three-dimensional exploded schematic view of a three-way reagent card of the present application.
FIG. 2 is a schematic top view of the lower member of the reagent card of the present application (1 strip is shown).
FIG. 3 is a schematic view of the lower surface of the upper member of the reagent card of the present application.
FIG. 4 is a schematic three-dimensional design of the nucleic acid detecting apparatus of the present application.
FIG. 5 is a schematic three-dimensional exploded view of the reagent card incubator of the present application.
FIG. 6 is a schematic cross-sectional view of a nucleic acid detecting apparatus of the present application.
The reference numerals in fig. 1-6 are illustrated as follows:
A cap 100;
A reagent card upper member 200, a sample application well assembly 201, a window assembly 202, a window film 203, a bevel platen assembly 204, a second platen assembly 205;
A reagent card lower member 300, a reaction chamber 301, a first horizontal chamber 302, a sloped channel 303, a test paper support member 304;
Test strip 400;
The reagent card incubator 500, the reagent card incubator upper housing 501, the first mounting hole 5011, the second bar slot 5012, the lithium battery 502, the vibration motor 503, the temperature control circuit board 504, the heating seat assembly 505, the reagent card incubator lower cover 506, and the fastening structure 507.
Detailed Description
The objects, technical solutions and advantages of the present application will become more apparent by the following detailed description of the present application with reference to the accompanying drawings. It should be understood that the description is only illustrative and is not intended to limit the scope of the application. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the present application.
Example 1A three-way reagent card
Fig. 1 shows a schematic exploded design of a three-way reagent card. A three-channel reagent card comprising: cap 100, reagent card upper member 200, reagent card lower member 300, test strip 400. The upper surface of reagent card upper portion component 200 is protruding to be provided with application of sample hole subassembly 201, window subassembly 202, window pad pasting 203, application of sample hole subassembly 201 is provided with 3 application of sample holes that link up respectively from top to bottom, window subassembly 202 is provided with 3 bar window grooves, is provided with window pad pasting 203 at window subassembly 202's upper surface seal.
Fig. 2 schematically shows the structure of the reagent card lower member 300. The reagent card lower member 300 includes: 3 reagent channels, each reagent channel includes according to the direction of solution flow in proper order: a reaction chamber 301, a first horizontal chamber 302, a bevel channel 303 and a test paper supporting member 304; the bottom surface of the first horizontal chamber 302 is lower than the bottom surface of the reaction chamber 301, and the height of the inclined surface channel is gradually increased along the advancing direction of the solution.
Fig. 3 schematically shows the lower surface structure of the reagent card upper member 200. The sample adding hole assembly 201 protrudes from the lower surface of the upper member 200 of the reagent card, and 3 sample adding holes correspond to 3 reaction chambers 301; a bevel platen assembly 204 and a second platen assembly 205 are also disposed on the lower surface of the reagent card upper member 200, the bevel platen assembly 204 corresponding to the bevel channel 303 (2 parallel bevel platens of the bevel platen assembly 204 are disposed inside the sidewall plate of the bevel channel 2), and the second platen assembly 205 corresponding to the test paper support member 304. The lower surface of the test strip 400 is placed on the inclined surface channel 303 and the test strip supporting member 304, and the upper surface of the test strip 400 is pressed by the inclined surface pressing plate 204 and the second pressing plate assembly 205, that is, the test strip 400 is fixed by the cooperation of the inclined surface channel 303, the test strip supporting member 304, the inclined surface pressing plate 204 and the second pressing plate assembly 205.
The test strip 400 displays the result in the strip window.
As shown in fig. 1 and 2, an opening is also provided in the reagent card lower member 300, in which a filter cartridge 305 is provided to avoid the influence of aerosols.
< Example 2 > a nucleic acid detecting apparatus
Fig. 4 schematically shows the construction of the detection device. A detection device comprising the reagent card of example 1 and a reagent card incubator 500.
Fig. 5 schematically shows the construction of a reagent card incubator 500. The reagent card incubator 500 includes: a reagent card incubator upper housing 501, a reagent card incubator lower cover 506.
In the inner space formed by the reagent card incubator upper housing 501 and the reagent card incubator lower cover 506, there are placed: lithium battery 502, vibration motor 503, temperature control circuit board 504, and heating base assembly 505; a heating block 505 and a vibration motor 503 are provided on the temperature control circuit board 504.
The reagent card incubator upper housing 501 is provided with a first mounting hole 5011 and a second bar slot 5012.
The heater block assembly 505 includes: the heating device comprises 3 heating seats and a spring guide mechanism, wherein the 3 heating seats are connected into a whole, one end of the spring guide mechanism is connected with the heating seats, and the other end of the spring guide mechanism is connected with a temperature control circuit board 504.
The vibration motor 503 is adapted to the first mounting hole 5011, and the vibration motor 503 is used for providing a vibration mixing function.
The lithium battery 502 supplies power to the temperature control circuit board 504, the vibration motor 503 and the heating seat.
The temperature control circuit board is used for controlling whether the heating seat and the vibration motor 503 work.
The 3 reaction chambers 301 correspond to the 3 heating seats, and the temperature of the reaction chambers is kept to be proper by the heating seats.
As shown in fig. 6, the reagent card upper member 200 of the three-channel reagent card and the reagent card incubator 500 are detachably connected, and only the reagent card of the three-channel reagent card needs to be replaced after the detection is completed.
Specifically, the three-channel reagent card and reagent card incubator 500 is provided with a mating quick release snap structure 507 for installation.
The working method of the nucleic acid detecting device of the application is as follows:
a, opening the cap 100, and adding a sample into the sample adding hole assembly 201:
b, the sample flows into the reaction bin along the sample adding hole assembly 201;
c, opening the temperature control circuit board 504 to keep the temperature of the reaction tank at a certain temperature;
d, after the step c is finished, adding diluent into the sample adding hole assembly 201;
e, the solution part of the reaction chamber flows to the first horizontal chamber 302 and then flows to the test strip 400 arranged on the inclined surface channel 303, and then the result is displayed in the strip-shaped window groove.
The above examples are provided for convenience of description of the present application and are not to be construed as limiting the application in any way, and any person skilled in the art will make partial changes or modifications to the application by using the disclosed technical content without departing from the technical features of the application.
Claims (5)
1. A split multichannel reagent card, comprising: a reagent card upper member, a reagent card lower member, n test strips;
The upper surface of the upper component of the reagent card is provided with a sample adding hole component, a window component and a window film in a protruding mode, the sample adding hole component is provided with n sample adding holes which are respectively communicated up and down, the window component is provided with n strip-shaped window grooves, and the upper surface of the window component is provided with the window film in a sealing mode; n is a natural number of 2 or more; the lower surface of the upper component of the reagent card is also provided with an inclined surface pressing plate component and a second pressing plate component;
Wherein the reagent card lower member comprises: the n reagent channels, each reagent channel includes in proper order according to the direction of solution flow: the reaction bin, the first horizontal bin, the inclined plane channel and the test paper supporting member; the bottom surface of the first horizontal bin is lower than the bottom surface of the reaction bin, and the height of the inclined plane channel is gradually increased along the advancing direction of the solution;
wherein, the cooperation relation of reagent card upper portion component, reagent card lower part component, test strip is:
The sample adding holes a and n correspond to the reaction bins n, the inclined plane pressing plate assembly corresponds to the inclined plane channel, and 2 parallel inclined plane pressing plates of the inclined plane pressing plate assembly are arranged on the inner side of a side wall plate of the inclined plane channel;
b, the second pressing plate assembly corresponds to the test paper supporting member;
c, the lower surface of the test strip is placed on the inclined surface channel and the test strip supporting member, and the upper surface of the test strip is pressed by the inclined surface pressing plate and the second pressing plate assembly;
and d, displaying a result on the strip-shaped window groove by the test strip.
2. The split multichannel reagent card of claim 1, wherein the well assembly protrudes from the upper and lower surfaces of the reagent card upper member.
3. A split multichannel reagent card according to claim 1, wherein the reagent card lower member is further provided with an opening in which the cartridge is disposed.
4. The split multichannel reagent card of claim 1, further comprising a cap; the cap is used for closing the upper surfaces of n loading holes of the loading hole assembly.
5. A nucleic acid detecting apparatus employing the split multichannel reagent card and the reagent card incubator according to any one of claims 1 to 4;
Wherein, reagent card incubator includes: an upper shell and a lower cover of the reagent card incubator;
Wherein, place in the interior space that reagent card incubator upper casing and reagent card incubator lower cover body formed: the lithium battery, the vibration motor, the temperature control circuit board and the heating seat component;
Wherein, the heating seat component and the vibration motor are arranged on the temperature control circuit board;
The upper shell of the reagent card incubator is provided with a first mounting hole and a second bar-shaped groove;
wherein, the heating seat subassembly includes: the heating device comprises n heating seats and a spring guide mechanism, wherein the n heating seats are connected into a whole, one end of the spring guide mechanism is connected with the heating seats, and the other end of the spring guide mechanism is connected with a temperature control circuit board;
Wherein the vibration motor is adapted to the first mounting hole;
The lithium battery supplies power to the temperature control circuit board, the heating seat and the vibration motor;
the temperature control circuit board is used for controlling whether the heating seat and the vibration motor work or not;
Wherein, the n reaction bins of the upper component of the reagent card correspond to the n heating seats of the incubator of the reagent card.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322606131.1U CN220887529U (en) | 2023-09-25 | 2023-09-25 | Split type multichannel reagent card and nucleic acid detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322606131.1U CN220887529U (en) | 2023-09-25 | 2023-09-25 | Split type multichannel reagent card and nucleic acid detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220887529U true CN220887529U (en) | 2024-05-03 |
Family
ID=90880089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322606131.1U Active CN220887529U (en) | 2023-09-25 | 2023-09-25 | Split type multichannel reagent card and nucleic acid detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220887529U (en) |
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2023
- 2023-09-25 CN CN202322606131.1U patent/CN220887529U/en active Active
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