CN216247997U

CN216247997U - Single-sample-adding-hole five-joint-detection immune colloidal gold and fluorescence immune card box

Info

Publication number: CN216247997U
Application number: CN202122859744.7U
Authority: CN
Inventors: 张利清; 莫维克
Original assignee: Jiannuo Biotechnology Jiangsu Co ltd
Current assignee: Jiannuo Biotechnology Jiangsu Co ltd
Priority date: 2021-11-22
Filing date: 2021-11-22
Publication date: 2022-04-08
Anticipated expiration: 2031-11-22

Abstract

The utility model discloses a single-sample-adding-hole five-joint-detection immune colloidal gold and fluorescence immune card box, which comprises an upper shell and a lower shell, wherein a sample diffusion part and five test slots which are arranged in parallel are arranged in the lower shell, and the upper shell is provided with a sample adding port matched with the sample diffusion part and five observation windows matched with the test slots.

Description

Single-sample-adding-hole five-joint-detection immune colloidal gold and fluorescence immune card box

Technical Field

The utility model relates to the field of testing, in particular to a single-sample-adding-hole five-joint-detection immune colloidal gold and fluorescence immune card box.

Background

The colloidal gold labeling technology is a novel immunolabeling technology which takes colloidal gold as a tracer marker and is applied to antigen-antibody reaction. The detection principle is that after a sample to be detected is added on a sample pad at one end of a test strip, the sample moves forwards through capillary action, colloidal gold labeled reagents on a binding pad are dissolved and then react with each other, and when the sample moves to a region of fixed antigen or antibody, the binding substance of the object to be detected and the gold labeled reagents is specifically bound with the binding substance and is intercepted, and the binding substance is gathered on a detection strip to generate a color development effect. Immunofluorescence is a method combining an immunological method (antigen-antibody specific binding) with a fluorescent labeling technology, and the detection principle is that an object to be detected in a sample and a fluorescent labeling antibody form an immune complex and are respectively solidified in a detection area and a quality control area through a chromatography process; when the reagent card reaches the detection area, the excitation light source of the analyzer emits light waves to irradiate the detection area and the quality control area of the reagent card, the solidified fluorescence immune antigen-antibody compound is excited, and the content of the analyzed substance can be calculated after the light waves emitted by the fluorescent substance are collected and converted into electric signals.

At present, the current immunity test detection card of the quintuplet card box is in a form that a plurality of sample adding holes correspond to a plurality of detection test strips and are integrated on one card box, different detection items of each detection card need to be added to a sample adding port in sequence, operation times are increased, operation time is long, and the requirement of simultaneous detection of a plurality of items can not be met under the condition that the content of a precious sample is low. Therefore, the development of an immune colloidal gold and immune fluorescent detection card box which has a simple structure and convenient use and can accurately measure a plurality of items at one time is a problem to be solved urgently.

Disclosure of Invention

The purpose of the utility model is as follows: the utility model aims to provide a single-sample-adding-hole five-joint-detection immune colloidal gold and a fluorescence immune card box aiming at the defects of the prior art.

The technical scheme is as follows: the utility model discloses a single-sample-adding-hole five-joint-detection immune colloidal gold and fluorescence immune card box which comprises an upper shell and a lower shell, wherein a sample diffusion part and five test grooves which are arranged in parallel are arranged in the lower shell, and a sample adding port matched with the sample diffusion part and five observation windows matched with the test groove are formed in the upper shell.

Preferably, the sample diffusion part includes a semicircular baffle plate and a glass fiber wool diffusion layer installed inside the semicircular baffle plate.

Preferably, the sample addition port comprises a double-layer oval sample addition port, and the bottom of the double-layer oval sample addition port is provided with a fixed reinforcing rib matched with the glass fiber cotton diffusion layer.

Preferably, the test slot comprises a reagent paper slot and a clamping slot.

Preferably, the bottom of the reagent paper groove is provided with a lower support block for supporting the reagent paper therein, and the upper shell is provided with an upper support block matched with the lower support block.

Compared with the prior art, the utility model has the following beneficial effects: the utility model provides a design of single application of sample hole quintuplet joint inspection immune colloidal gold and fluorescence immune card box, this design only has 1 sample addition mouth, detect at every turn and only need add about 400 mu L the sample can accomplish 5 test groove regional reactions, each test groove testing result homogeneity is good after the reaction, this technical scheme simple structure, the cost of manufacture is low, compare in current more add application of sample hole quintuplet card box operation more convenient, sample utilization rate is higher, application prospect is wide.

Drawings

FIG. 1 is a schematic diagram of the structure of the upper housing part of a single well five-joint test immune colloidal gold and fluorescence immune cassette of the present invention;

FIG. 2 is a schematic structural diagram of a lower housing part of a single well five-joint test immuno-colloidal gold and a fluorescent immuno-cartridge according to the present invention.

In the figure: 1. an upper housing; 101. fixing the reinforcing ribs; 102. a sample addition port; 103. an observation window; 104. an upper support block; 2. a lower housing; 201. a semicircular baffle plate; 202. a clamping groove; 203. a lower support block; 204. reagent paper groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," "circumferential," and the like are used in the indicated orientations and positional relationships based on the drawings for convenience in describing and simplifying the description, but do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the utility model.

In the present invention, unless otherwise specifically stated or limited, the terms "mounted," "connected," "fixed," and the like are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; the connection can be mechanical connection, electrical connection or communication connection; either directly or indirectly through intervening media, either internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

The technical solution of the present invention will be described in detail below with specific examples. The following several specific embodiments may be combined with each other, and details of the same or similar concepts or processes may not be repeated in some embodiments.

The utility model provides a five joint inspection immune colloidal gold of single application of sample hole and fluorescence immune card box, includes casing 1 and lower casing 2, is equipped with sample diffusion portion and five test groove that set up side by side in the casing 2 down, goes up and sets up sample addition port 102 and five observation windows 103 of cooperation test groove position that cooperate the sample diffusion portion position on the casing 1. The technical scheme has the advantages that the design of the single sample adding hole five-joint detection immune colloidal gold and fluorescence immune card box is provided, only 1 sample adding port 102 is provided, only about 400 mu L of sample needs to be added for each detection to complete the reaction of 5 test groove areas, the uniformity of the detection result of each test groove is good after the reaction is finished, and the technical scheme is simple in structure and low in manufacturing cost, and is more convenient to operate, higher in sample utilization rate and wide in application prospect compared with the existing multi-sample adding hole five-joint detection card box.

Specifically, the sample diffusion portion includes semicircular baffle 201 and installs the glass fiber cotton diffusion layer in semicircular baffle 201, sample addition port 102 includes double-deck oval sample addition port 102, double-deck oval sample addition port 102's bottom be equipped with glass fiber cotton diffusion layer complex fixed strengthening rib 101, the test groove includes reagent paper groove 204 and joint groove 202, the bottom in reagent paper groove 204 is equipped with the lower support piece 203 that is used for supporting wherein reagent paper, go up be equipped with on the casing 1 with lower support piece 203 complex support piece 104.

In specific implementation, the upper shell 1 and the lower shell 2 are clamped, the length, the width and the height of each of the upper shell 1 and the lower shell 2 are 49 × 110 × 5mm, the sample diffusion part comprises a semicircular baffle 201 and a glass fiber cotton diffusion layer arranged in the semicircular baffle 201, the absorption and the diffusion of a sample after sample injection are carried out by matching with the sample injection port 102, the size of the semicircular baffle 201 is 38.5 × 10.5mm, the reagent paper groove 204 comprises a groove body and reagent paper arranged in the groove body, the reagent paper comprises immune colloidal gold and an immune fluorescent test strip, the sample adding port 102 is a double-layer oval sample adding port 102 and comprises two invaginated ovals, wherein outer oval size is 25 × 8mm, inner oval size is 8 × 5mm, observation window 103 comprises 5 independent small window that the interval is 5mm, the length and width of every small window is 15 × 3.2mm, joint groove 202 structure is used for adapting detecting instrument, contains the joint inspection card box of the same structure different width all can operate on specific detecting instrument.

The single-sample-hole five-joint-detection immune colloidal gold and fluorescence immune card box realized by adopting the embodiment has the following advantages: 1. the sample is added at a single port, and 5 test strip detections are realized by one-time sample addition, so that the operation time and the detection sample are saved; 2. the card slot designed on the lower shell 2 can be matched with the sample adding port 102 of the instrument, so that the function of detecting card shells with different specifications by the same sample adding port 102 is realized; 3. the semi-elliptical glass fiber cotton design can enable a sample to be uniformly transmitted to the detection test paper, the reaction rates of 5 pieces of test paper are basically consistent, and the obtained results are not different.

In the present invention, unless otherwise explicitly specified or limited, the first feature "on" or "under" the second feature may be directly contacting the first feature and the second feature or indirectly contacting the first feature and the second feature through an intermediate. Also, a first feature "on," "above," and "over" a second feature may mean that the first feature is directly above or obliquely above the second feature, or that only the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lower level than the second feature. In the description herein, reference to the description of the term "one embodiment," "some embodiments," "an example," "a specific example" or "some examples," or the like, means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example.

Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the utility model has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. The utility model provides a five joint inspection immune colloidal gold and fluorescence immune card boxes in single application of sample hole, includes casing and lower casing, its characterized in that: the lower casing is internally provided with a sample diffusion part and five test slots arranged in parallel, and the upper casing is provided with a sample adding port matched with the sample diffusion part and five observation windows matched with the test slots.

2. The single-well five-up test immune colloidal gold and fluorescent immune cartridge according to claim 1, wherein: the sample diffusion part comprises a semicircular baffle plate and a glass fiber cotton diffusion layer arranged in the semicircular baffle plate.

3. The single-well five-up test immune colloidal gold and fluorescent immune cartridge according to claim 2, wherein: the sample adding port comprises a double-layer oval sample adding port, and the bottom of the double-layer oval sample adding port is provided with a fixed reinforcing rib matched with the glass fiber cotton diffusion layer.

4. The single-well five-up test immune colloidal gold and fluorescent immune cartridge according to claim 1, wherein: the test slot comprises a reagent paper slot and a clamping slot.

5. The single-well five-up test immuno-colloidal gold and fluoroimmunoassay cartridge of claim 4, wherein: the bottom of the reagent paper groove is provided with a lower supporting block for supporting the reagent paper therein, and the upper shell is provided with an upper supporting block matched with the lower supporting block.