CN220788539U - Internal and external double-circulation bioreactor - Google Patents
Internal and external double-circulation bioreactor Download PDFInfo
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- CN220788539U CN220788539U CN202322359418.9U CN202322359418U CN220788539U CN 220788539 U CN220788539 U CN 220788539U CN 202322359418 U CN202322359418 U CN 202322359418U CN 220788539 U CN220788539 U CN 220788539U
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 44
- 238000000855 fermentation Methods 0.000 claims abstract description 37
- 230000004151 fermentation Effects 0.000 claims abstract description 37
- 230000003287 optical effect Effects 0.000 claims abstract description 13
- 230000007246 mechanism Effects 0.000 claims abstract description 9
- 238000004140 cleaning Methods 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims description 7
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000002640 oxygen therapy Methods 0.000 claims description 2
- 230000003749 cleanliness Effects 0.000 abstract description 4
- 230000000903 blocking effect Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 description 24
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 239000007921 spray Substances 0.000 description 8
- 229910002092 carbon dioxide Inorganic materials 0.000 description 6
- 239000001569 carbon dioxide Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 210000004748 cultured cell Anatomy 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides an internal and external double-circulation bioreactor, which relates to the technical field of biological test equipment and comprises the following components: the fermentation cylinder, the bottom fixedly connected with conical plate of fermentation cylinder, the bottom fixedly connected with L type connecting pipe of conical plate, be equipped with on the L type connecting pipe and block up clean mechanism, the one end fixedly connected with water tank of L type connecting pipe, the top fixedly connected with water inlet of water tank, the one end fixedly connected with water pipe of water tank, the one end fixedly connected with booster pump of water pipe, one side fixedly connected with outlet pipe of booster pump, the one end of outlet pipe runs through the top at the fermentation cylinder. According to the utility model, the problem of low cleanliness of the bioreactor is solved by the blocking cleaning mechanism, when residues on the inner wall of the fermentation tank are required to be cleaned, the booster pump is only required to be closed, and then the optical axis is manually rotated ninety degrees, so that the residues flow out of the inner wall of the L-shaped connecting pipe, and the cleanliness of the bioreactor is realized.
Description
Technical Field
The utility model relates to the technical field of biological test equipment, in particular to an internal and external double-circulation bioreactor.
Background
An internal and external double-circulation bioreactor is a device for carrying out biological reaction, wherein the bioreactor is commonly called a fermentation tank, and the main function of the bioreactor is to provide proper environmental conditions for microorganisms or cells to grow, metabolize and produce target products, two circulation systems are arranged in the internal and external double-circulation bioreactor, one is an internal circulation, the other is an external circulation, the internal circulation of cell culture is carried out by using ventilation through oxygen, the external circulation is used for introducing nutrient solution into the fermentation tank, and excessive nutrient solution flows out of a culture medium for recycling, but excessive nutrient solution possibly adheres to the inner wall of a recycling pipe with residual suction cup in the recycling process, so that the internal and external double-circulation bioreactor is required to be provided.
Disclosure of Invention
In order to achieve the above purpose, the present utility model adopts the following technical scheme: a bioreactor for internal and external dual circulation, comprising: the fermentation cylinder, the bottom fixedly connected with conical plate of fermentation cylinder, the bottom fixedly connected with L type connecting pipe of conical plate, be equipped with on the L type connecting pipe and block up clean mechanism, the one end fixedly connected with water tank of L type connecting pipe, the top fixedly connected with water inlet of water tank, the one end fixedly connected with water pipe of water tank, the one end fixedly connected with booster pump of water pipe, one side fixedly connected with outlet pipe of booster pump, the one end of outlet pipe runs through on the top of fermentation cylinder, the one end fixedly connected with shower nozzle of outlet pipe.
As a preferred implementation mode, the top end of the fermentation tank is fixedly connected with an oxygen delivery pipe, and one end, close to the bottom end, of the fermentation tank is fixedly connected with an air outlet pipe.
The technical effect of adopting the further scheme is as follows: when the staff needs to spray the nutrient solution to the cultured cells in the fermentation cylinder, firstly, let in the nutrient solution through the water inlet, then the nutrient solution of water tank inner wall is because of being in static state, so get into the inner wall of conical plate from L type connecting pipe, the one end that is L type outlet duct is plugged up afterwards, make the fermentation cylinder inner wall be airtight space, the staff starts the booster pump, enter into shower nozzle one side through water pipe and outlet pipe, utilize spray principle at last, supply nutrition to the cell, unnecessary nutrient solution can follow and pile up at conical plate inner wall, continue by booster pump cyclic utilization, when need take a breath for the fermentation cylinder, only need to the oxygen catheter on top can, can pile up in conical plate one side because carbon dioxide density is greater than oxygen, then follow the outlet duct and flow out (after the premise booster pump starts, the nutrient solution can't be higher than the height of conical plate, carbon dioxide can not by water barrier), thereby get into the outlet duct).
As a preferred implementation mode, the blockage cleaning mechanism comprises a round filter plate, the inner wall of an L-shaped connecting pipe is fixedly connected with the round filter plate, one end of the L-shaped connecting pipe is fixedly connected with a drain pipe, the inner wall of the drain pipe is rotationally connected with a hemispherical body, one side of the hemispherical body is fixedly connected with an optical axis, and one end of the optical axis is connected with one end of the drain pipe in a penetrating manner.
The technical effect of adopting the further scheme is as follows: when the residue of clean L type connecting pipe inner wall is needed, the staff only need close the booster pump, then block the residue because of circular filter, simultaneously because of the flow of water, the residue can automatic sliding blow off pipe inner wall pile up, and manual rotatory optical axis ninety degrees makes the hemisphere open the blow off pipe inner wall, drives the residue through the nutrient solution and flows out, and then reverse rotation optical axis ninety degrees can, if need change the nutrient solution when, the staff only need open through rotatory hemisphere.
As a preferred implementation mode, two circular notches are formed in one end of the fermentation tank, a rotating shaft is slidably connected to the inner wall of each circular notch, a connecting plate is fixedly connected to one end of each rotating shaft, a sector plate is fixedly connected to one side of each connecting plate, a filter plate is fixedly connected to one side of each sector plate, and four right culture medium grooves are formed in one side of each filter plate.
The technical effect of adopting the further scheme is as follows: when the cells need to be placed, the staff firstly puts the cells or vegetation on one side of the filter plate, stores the cells or vegetation through the culture medium groove, then inserts the rotating shafts on two sides of the filter plate into the inner walls of the two circular notches at one end of the fermentation tank, and simultaneously ensures the air tightness of the inner wall of the fermentation tank, and is convenient to install.
As a preferred embodiment, four supporting feet are fixedly connected to one side of the conical plate.
The technical effect of adopting the further scheme is as follows: the fermentation tank is supported by four supporting feet at the bottom end.
Compared with the prior art, the utility model has the advantages and positive effects that,
according to the bioreactor disclosed by the utility model, the problem of low cleanliness of the bioreactor is solved through the blockage cleaning mechanism, when residues on the inner wall of the fermentation tank are required to be cleaned, only the booster pump is required to be turned off, then the optical axis is manually rotated ninety degrees, so that the residues flow out of the inner wall of the L-shaped connecting pipe, and when the sucker or vegetation is required to be placed and replaced, only the connecting plate is required to be manually pulled back and forth, so that the cleanliness of the bioreactor is simply increased through the operation steps.
Drawings
Fig. 1 is a schematic perspective view of an external and internal double circulation bioreactor according to the present utility model.
Fig. 2 is a schematic diagram of a split three-dimensional structure of an internal and external double-circulation bioreactor provided by the utility model.
Fig. 3 is an isometric view of an internal and external double circulation bioreactor according to the present utility model.
Fig. 4 is a schematic perspective view of a water tank of a bioreactor with internal and external double circulation.
Fig. 5 is a schematic top perspective view of an internal and external double circulation bioreactor according to the present utility model.
Legend description:
1. a fermentation tank; 2. a conical plate; 3. an L-shaped connecting pipe; 4. a water tank; 5. a water pipe; 6. a booster pump; 7. a water outlet pipe; 8. a spray head; 9. supporting feet; 10. a water inlet; 11. a circular filter plate; 12. a blow-down pipe; 13. an oxygen therapy tube; 14. an air outlet pipe; 15. a circular slot; 16. a rotating shaft; 17. a connecting plate; 18. a sector plate; 19. a filter plate; 20. a culture medium tank; 21. a hemisphere; 22. an optical axis.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-5, the present utility model provides a technical solution: an internal and external double circulation bioreactor comprising: fermentation cylinder 1, the bottom fixedly connected with conical plate 2 of fermentation cylinder 1, the bottom fixedly connected with L type connecting pipe 3 of conical plate 2, be equipped with on the L type connecting pipe 3 and block up clean mechanism, the one end fixedly connected with water tank 4 of L type connecting pipe 3, the top fixedly connected with water inlet 10 of water tank 4, the one end fixedly connected with water pipe 5 of water tank 4, the one end fixedly connected with booster pump 6 of water pipe 5, one side fixedly connected with outlet pipe 7 of booster pump 6, the one end of outlet pipe 7 runs through on the top of fermentation cylinder 1, the one end fixedly connected with shower nozzle 8 of outlet pipe 7.
As shown in fig. 1-5, the top end of the fermenter 1 is fixedly connected with an oxygen pipe 13, one end of the fermenter 1 close to the bottom end is fixedly connected with an air outlet pipe 14, when a worker needs to spray nutrient solution to cultured cells in the fermenter 1, the nutrient solution is firstly introduced through the water inlet 10, then the nutrient solution on the inner wall of the water tank 4 is in a static state, so that the nutrient solution enters the inner wall of the conical plate 2 from the L-shaped connecting pipe 3, then one end of the L-shaped air outlet pipe 14 is blocked, the inner wall of the fermenter 1 is made to be a closed space, the worker starts the booster pump 6, enters one side of the spray head 8 through the water pipe 5 and the water outlet pipe 7, finally, the nutrient solution is supplemented by utilizing a spraying principle, and the redundant nutrient solution is continuously recycled by the booster pump 6, when the fermentation tank 1 needs to be ventilated, only the oxygen pipe 13 at the top end is required to be used for gas transmission, and the carbon dioxide is accumulated on one side of the conical plate 2, and then flows out from the air outlet pipe 14 (after the booster pump 6 is started, the nutrient solution cannot be higher than the height of the conical plate 2, so that the carbon dioxide cannot be blocked by water and enters the air outlet pipe 14).
As shown in fig. 1-5, the blocking cleaning mechanism comprises a circular filter plate 11, the inner wall of an L-shaped connecting pipe 3 is fixedly connected with the circular filter plate 11, one end of the L-shaped connecting pipe 3 is fixedly connected with a drain pipe 12, the inner wall of the drain pipe 12 is rotationally connected with a hemisphere 21, one side of the hemisphere 21 is fixedly connected with an optical axis 22, one end of the optical axis 22 is connected with one end of the drain pipe 12 in a penetrating way, when residues on the inner wall of the L-shaped connecting pipe 3 need to be cleaned, a worker only needs to close a booster pump 6, then the residues are blocked by the circular filter plate 11, meanwhile, the residues can automatically slide and accumulate on the inner wall of the drain pipe 12 due to the flowing of water, the optical axis 22 is manually rotated for ninety degrees, the hemisphere 21 is opened on the inner wall of the drain pipe 12, the residues are driven by nutrient solution, and then the optical axis 22 ninety degrees is reversely rotated.
As shown in fig. 1-5, two circular notches 15 are formed at one end of the fermentation tank 1, a rotating shaft 16 is slidably connected to the inner wall of the circular notches 15, a connecting plate 17 is fixedly connected to one end of the rotating shaft 16, a sector plate 18 is fixedly connected to one side of the connecting plate 17, a filter plate 19 is fixedly connected to one side of the sector plate 18, four right culture medium grooves 20 are formed in one side of the filter plate 19, when cells are required to be placed, workers firstly place the cells or vegetation on one side of the filter plate 19, store the cells or vegetation through the culture medium grooves 20, and then insert the rotating shafts 16 on two sides of the filter plate 19 into the inner walls of the two circular notches 15 at one end of the fermentation tank 1, and meanwhile, the air tightness of the inner wall of the fermentation tank 1 is guaranteed, and the installation is convenient.
As shown in fig. 1-5, one side of the conical plate 2 is fixedly connected with four supporting feet 9, and the fermentation tank 1 is supported by the four supporting feet 9 at the bottom end.
Working principle: when a worker needs to spray nutrient solution to cultured cells in the fermentation tank 1, nutrient solution is introduced through the water inlet 10 at the top of the water tank 4, if the booster pump 6 is not started, the nutrient solution enters the inner wall of the conical plate 2 from the L-shaped connecting pipe 3 and then one end of the L-shaped air outlet pipe 14 is blocked, so that the inner wall of the fermentation tank 1 is a closed space, after the booster pump 6 is started, the nutrient solution moves from the water tank 4 and the L-shaped connecting pipe 3 into the water outlet pipe 7 and then is sprayed out from one side of the spray head 8 to perform irrigation work, then the contained nutrient solution penetrates through the filter plate 19 and is accumulated in the conical plate 2, the booster pump 6 drives one side of the spray head 8 in a reciprocating cycle, after the booster pump 6 is started, ventilation work can be performed for the fermentation tank 1, oxygen is firstly conveyed to the oxygen conveying pipe 13 at the top end, and carbon dioxide can be accumulated on one side of the conical plate 2 because the nutrient solution is circularly flowed, and the carbon dioxide cannot flow out of the air outlet pipe 14 because the horizontal plane is lower than one side of the conical plate 2.
The present utility model is not limited to the above-mentioned embodiments, and any equivalent embodiments which can be changed or modified by the technical content disclosed above can be applied to other fields, but any simple modification, equivalent changes and modification made to the above-mentioned embodiments according to the technical substance of the present utility model without departing from the technical content of the present utility model still belong to the protection scope of the technical solution of the present utility model.
Claims (5)
1. A bioreactor for internal and external double circulation, comprising: fermentation cylinder (1), the bottom fixedly connected with tapered plate (2) of fermentation cylinder (1), the bottom fixedly connected with L type connecting pipe (3) of tapered plate (2), be equipped with on L type connecting pipe (3) and block up clean mechanism, the one end fixedly connected with water tank (4) of L type connecting pipe (3), the top fixedly connected with water inlet (10) of water tank (4), the one end fixedly connected with water pipe (5) of water tank (4), the one end fixedly connected with booster pump (6) of water pipe (5), one side fixedly connected with outlet pipe (7) of booster pump (6), the one end of outlet pipe (7) runs through the top at fermentation cylinder (1), the one end fixedly connected with shower nozzle (8) of outlet pipe (7).
2. An internal and external double circulation bioreactor according to claim 1, wherein: the top of fermentation cylinder (1) fixedly connected with oxygen therapy pipe (13), the one end fixedly connected with outlet duct (14) that fermentation cylinder (1) is close to the bottom.
3. An internal and external double circulation bioreactor according to claim 1, wherein: the blockage cleaning mechanism comprises a round filter plate (11), the inner wall of an L-shaped connecting pipe (3) is fixedly connected with the round filter plate (11), one end of the L-shaped connecting pipe (3) is fixedly connected with a drain pipe (12), the inner wall of the drain pipe (12) is rotationally connected with a hemispherical body (21), one side of the hemispherical body (21) is fixedly connected with an optical axis (22), and one end of the optical axis (22) is connected with one end of the drain pipe (12) in a penetrating mode.
4. An internal and external double circulation bioreactor according to claim 2, wherein: two circular notch (15) have been seted up to one end of fermentation cylinder (1), the inner wall sliding connection of circular notch (15) has pivot (16), the one end fixedly connected with connecting plate (17) of pivot (16), one side fixedly connected with sector plate (18) of connecting plate (17), one side fixedly connected with filter (19) of sector plate (18), four right culture medium grooves (20) are seted up to one side of filter (19).
5. An internal and external double circulation bioreactor according to claim 1, wherein: four supporting feet (9) are fixedly connected to one side of the conical plate (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322359418.9U CN220788539U (en) | 2023-08-31 | 2023-08-31 | Internal and external double-circulation bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322359418.9U CN220788539U (en) | 2023-08-31 | 2023-08-31 | Internal and external double-circulation bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220788539U true CN220788539U (en) | 2024-04-16 |
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ID=90656572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322359418.9U Active CN220788539U (en) | 2023-08-31 | 2023-08-31 | Internal and external double-circulation bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220788539U (en) |
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2023
- 2023-08-31 CN CN202322359418.9U patent/CN220788539U/en active Active
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