CN220745930U - Portable collection device of anaerobic microorganism sample - Google Patents
Portable collection device of anaerobic microorganism sample Download PDFInfo
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- CN220745930U CN220745930U CN202322417064.9U CN202322417064U CN220745930U CN 220745930 U CN220745930 U CN 220745930U CN 202322417064 U CN202322417064 U CN 202322417064U CN 220745930 U CN220745930 U CN 220745930U
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- anaerobic
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- 244000005700 microbiome Species 0.000 title claims abstract description 46
- 238000005070 sampling Methods 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims abstract description 13
- 230000000813 microbial effect Effects 0.000 claims description 10
- 239000004033 plastic Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 239000007788 liquid Substances 0.000 abstract description 15
- 238000001914 filtration Methods 0.000 abstract description 5
- 238000004321 preservation Methods 0.000 abstract description 4
- 238000012216 screening Methods 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract description 3
- 230000005484 gravity Effects 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 230000000968 intestinal effect Effects 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 230000000694 effects Effects 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- -1 polyethylene Polymers 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 2
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 235000018417 cysteine Nutrition 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008482 dysregulation Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
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Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a portable collection device of anaerobic microorganism samples, which relates to the technical field of microorganism collection, and comprises an outer package, a self-sealing sample bag and an anaerobic microorganism sample collection device; the outer package adopts a sterile sealed transparent outer package; the self-sealing sample bag is used for accommodating the whole collecting device; the anaerobic microorganism sample collection device comprises a collection bottle cap, a collection bottle and a sample bottle; in the technical scheme provided by the utility model, when a microorganism sample is obtained, a proper amount of microorganism sample is dug by a sampling spoon and is filled into a sampling bottle filled with sample protection liquid; the air is removed completely through pressure operation, the sample is suspended and dissolved, and the suspended sample is transferred into a sample bottle through gravity filtration. And further, the collection of the anaerobic microorganism sample and the formation of an anaerobic storage environment are completed in a short time, which is favorable for the preservation and transportation of the anaerobic microorganism screening sample.
Description
Technical Field
The utility model relates to the technical field of microorganism collection, in particular to a portable collection device for anaerobic microorganism samples.
Background
In nature and various microenvironments, such as the bottom of a cultivation area, human intestinal canal and other spaces, anaerobic conditions with different degrees exist. In which a large number of anaerobic microorganisms are grown, which are generally sensitive to oxygen and whose activity decreases drastically upon contact with oxygen. However, these microorganisms play an important role in the natural matter energy cycle, the human nutrition conversion, the health maintenance, and the like.
Currently, intestinal flora transplantation is considered as an effective way to treat intestinal diseases and health problems caused by dysregulation of intestinal flora. As an important step of intestinal flora transplanting technology and research, the method for obtaining the high-quality microbial flora sample is a precondition for ensuring curative effect and wide research materials. The utility model discloses a human intestinal flora collection system, relates to intestinal flora and gathers technical field, including gathering the bag, gathering the seat, the upper portion of gathering the seat is equipped with adjacent first recess and second recess, first recess is used for placing and gathers the bag, the second recess is used for collecting urine, gather the seat upper surface and rotate and be connected with the packing ring, the outer fringe of packing ring is hugged closely with gathering the seat upper surface, the packing ring middle part is equipped with the window, the window overlaps with the opening of first recess and second recess simultaneously, still is equipped with back, first sample container, fluid-discharge tube, second sample container.
However, how to collect the corresponding sample quickly and store and transport under proper conditions, especially when obtaining the microbial sample, to remove the air cleanly and keep the original state of the sample is a problem to be solved.
Disclosure of Invention
The utility model aims to solve the technical problems of overcoming the defects of the prior art and providing a portable anaerobic microorganism sample collecting device, which can completely remove air through pressure operation, collect anaerobic microorganism samples in a short time and form an anaerobic storage environment, thereby being beneficial to the preservation and transportation of anaerobic microorganism screening samples.
In view of this, the technical scheme of the utility model is as follows:
the portable collection device of the anaerobic microorganism sample comprises an outer package, a self-sealing sample bag and the anaerobic microorganism sample collection device;
the outer package adopts a sterile sealed transparent outer package, and the outer surface of the outer package is printed with product names and using instructions;
the self-sealing sample bag is used for accommodating the whole collecting device;
anaerobic microorganism sample collection device includes collection bottle lid, collection bottle and sample bottle, collection bottle lid sets up in the collection bottle, collection bottle lid's top is through pipeline fixed mounting has discharge valve, collection bottle lid's bottom is provided with the sample spoon, collection bottle's lateral wall fixed mounting has the connecting pipe that is linked together, just the other end fixed mounting of connecting pipe is at the lateral wall of sample bottle.
Optionally, two ends of the outer package are arranged to be of a tearing structure.
Optionally, the collection bottle lid sets up to the toper structure of handstand, just the collection bottle lid is linked together with the collection bottle through the notch.
Optionally, a net-shaped filter is arranged on the side wall of the collecting bottle near the bottom, and the filter is communicated with the installed connecting pipe.
Optionally, one end of the connecting pipe is fixedly provided with a valve pipeline, the other end of the valve pipeline is fixedly arranged on the side wall of the sample bottle, and the connecting pipe is fixedly arranged on the side wall of the sample bottle through the valve pipeline.
Optionally, the collection bottle cap is fit on top of the collection bottle.
Optionally, the collection bottle and the sample bottle are both elastic plastic bottles.
From the above technical solutions, the embodiment of the present utility model has the following advantages:
when a microorganism sample is obtained, a proper amount of microorganism sample is dug by a sampling spoon and is filled into a sampling bottle filled with sample protection liquid; the air is removed completely through pressure operation, the sample is suspended and dissolved, and the suspended sample is transferred into a sample bottle through gravity filtration. And further, the collection of the anaerobic microorganism sample and the formation of an anaerobic storage environment are completed in a short time, which is favorable for the preservation and transportation of the anaerobic microorganism screening sample.
These features and advantages of the present utility model will be disclosed in detail in the following detailed description and the accompanying drawings.
Drawings
The utility model is further described with reference to the accompanying drawings:
FIG. 1 is a schematic view of the structure of an overwrap of the present utility model;
FIG. 2 is a schematic view of the structure of the self-sealing sample bag of the present utility model;
FIG. 3 is a diagram of an anaerobic microorganism sample collection device according to the present utility model;
FIG. 4 is a schematic view of the structure of the collecting bottle cap of the present utility model;
FIG. 5 is a schematic view of the structure of the collection bottle of the present utility model;
FIG. 6 is a schematic view of the structure of the sample bottle of the present utility model.
Reference numerals illustrate: 1. and (3) outer packaging; 2. an easy-to-tear structure; 3. product name; 4. instructions for use; 5. a self-sealing sample bag; 6. an exhaust valve; 7. collecting a bottle cap; 8. sampling spoon; 9. collecting a bottle; 10. a filter; 11. a connecting pipe; 12. a valve conduit; 13. sample bottles.
Detailed Description
The technical solutions of the embodiments of the present utility model will be explained and illustrated below with reference to the drawings of the embodiments of the present utility model, but the following embodiments are only preferred embodiments of the present utility model, and not all embodiments. Based on the examples in the implementation manner, other examples obtained by a person skilled in the art without making creative efforts fall within the protection scope of the present utility model.
A portable collection device for anaerobic microorganism samples according to an embodiment of the present utility model will be described in detail with reference to the accompanying drawings.
For easy understanding, referring to fig. 1 to 6, an embodiment of a portable anaerobic microorganism sample collection device provided by the present utility model includes an outer package 1, a self-sealing sample bag 5, and an anaerobic microorganism sample collection device;
the outer package 1 is a sterile sealed transparent outer package, and the outer surface of the outer package 1 is printed with a product name 3 and a use instruction 4;
the self-sealing sample bag 5 is used for accommodating the whole collecting device;
the anaerobic microorganism sample collection device comprises a collection bottle cap 7, a collection bottle 9 and a sample bottle 13, wherein the collection bottle cap 7 is arranged in the collection bottle 9, an exhaust valve 6 is fixedly arranged at the top of the collection bottle cap 7 through a pipeline, a sampling spoon 8 is arranged at the bottom of the collection bottle cap 7, a connecting pipe 11 communicated with the side wall of the collection bottle 9 is fixedly arranged on the side wall of the collection bottle 9, and the other end of the connecting pipe 11 is fixedly arranged on the side wall of the sample bottle 13; one end of the connecting pipe 11 is fixedly provided with a valve pipeline 12, the other end of the valve pipeline 12 is fixedly arranged on the side wall of the sample bottle 13, and the connecting pipe 11 is fixedly arranged on the side wall of the sample bottle 13 through the valve pipeline 12.
The outer package 1 is provided with a transparent self-sealing sample bag 5 and an anaerobic microorganism sample collecting device. The front end treatment device of the anaerobic microorganism sample collection device consists of an exhaust valve 6, a sampling spoon 8 and a collection bottle 9, the inside of the anaerobic microorganism sample collection device is filled with an indicating sample protection liquid (or diluent), the rear end of the anaerobic microorganism sample collection device is provided with a screw cap sample bottle 13 with the same volume, and a small amount of indicating sample protection liquid or diluent is contained in the anaerobic microorganism sample collection device. The two parts are connected through a section of valve pipeline 12 with a switch. When a microorganism sample is obtained, a proper amount of microorganism sample is dug by a sampling spoon 8 and is filled into a sampling bottle 9 filled with sample protection liquid; the air is removed by pressure operation, and the sample is suspended and dissolved, and the suspended sample is transferred into the sample bottle 13 by gravity filtration. Furthermore, the collection of anaerobic microorganism samples and the formation of anaerobic storage environment are completed in a short time, which is favorable for the preservation and transportation of anaerobic microorganism screening samples, the outer package 1 can be made of polyethylene PE material, the top end of the exhaust valve 6 is communicated with air, and the bottom end is communicated with the collection bottle 9 through the collection bottle cap 7;
the preset indicating protective liquid in the collecting device consists of a resazurin indicator with the final concentration of 50mg/L, a cysteine sodium salt with the final concentration of 500mg/L and 20% glycerol, and the total volume is slightly larger than the volume of a collecting bottle 9.
In some embodiments, as shown in fig. 1, two ends of the outer package 1 are provided as tear-off structures 2.
It should be noted that, the outer package 1 is conveniently disassembled by the easy-tearing structure 2.
In some embodiments, the collecting bottle cap 7 is provided with an inverted conical structure, and the through notch of the collecting bottle cap 7 is communicated with the collecting bottle 9; the side wall of the collecting bottle 9 is provided with a net-shaped filter 10 near the bottom, and the filter 10 is communicated with a connecting pipe 11 which is arranged.
The collecting bottle cap 7 is conical, the diameter of the lower part is small, the bottom end of the collecting bottle cap is connected with a spoon-shaped sampling spoon 8, and the filtering treatment is carried out through the arranged net-shaped filter 10, so that the microorganism is ensured to be filtered into the sample bottle 13.
The collecting bottle cap 7 is fit on the top of the collecting bottle 9; both the collection bottle 9 and the sample bottle 13 are elastic plastic bottles.
It should be noted that, guarantee to have good sealed effect between collection bottle lid 7 and the collection bottle 9, all adopt to have elastic plastic bottle through collection bottle 9 and sample bottle 13, conveniently gather and use.
Working principle: before the product is used, whether the outer package is complete or not is observed, whether the collecting device leaks liquid or not is checked through transparent packaging, and the tightness is checked; the protective liquid in the device can be used without being red or purple. Tearing the outer package from the easy-tearing structure, and taking out the self-sealing sample bag and the collecting device. Confirm that the vent valve 6 is in the closed state, open the valve on the valve conduit 12, tilt the whole device 90 ° to make the sample bottle 13 up, the indicative protective liquid fills the collection bottle 9, close the valve on the valve conduit 12. The device is vertically placed, the collecting bottle cap 7 is unscrewed, and a sampling spoon 8 is used for rapidly excavating a required target microorganism sample. The microbial sample is transferred to the indicative protective liquid of the collection bottle 9 through the sampling spoon 8, and the cover is screwed. The exhaust valve 6 is slightly opened by action, the collection bottle 9 is slightly squeezed, air possibly remained in the bottle is discharged, the indicative protection liquid at the top of the collection bottle slightly overflows, and the exhaust valve 6 is immediately closed. The valve on the valve pipe 12 is opened, and the sample bottle 13 is gently pressed, so that the gas part in the sample bottle enters the collection bottle 9. The valve on the valve pipeline 12 is closed, the collecting device is rocked, and the sample on the sampling spoon 8 is repeatedly suspended by the protection liquid. The valve on the valve pipeline 12 is opened, the device is inclined at 90 degrees, so that the sample bottle 13 is at the lower position, the microorganism sample suspension in the collection bottle 9 can pass through the filter 10 and enter the sample bottle 13 until the sample bottle 13 is full, and the valve on the valve pipeline 12 is closed. The sampling device is sealed and packed by a self-sealing sample bag, so that the sample is prevented from leaking, and the sample in the sample bottle 13 can be stored in a freezing way or transported according to the requirement
The device disclosed by the utility model is used for filling the indicating protective liquid in the anaerobic box and assembling the anaerobic box into a closed product, and meanwhile, the outer package 1 can ensure the long-term sterile state. In the indicating protective liquid, resazurin is an indicator of the oxygen concentration, and the lower the oxygen concentration is, the lighter the color is, and the colorless is when the protective liquid is completely free of oxygen. The freezing storage protective agent for microorganisms at 20% of glycerol can protect microbial cells during long-term freezing process. The cysteine sodium salt can consume trace oxygen dissolved into a sampling bottle during sampling, and maintain anaerobic conditions.
The conical collecting bottle cap 7 can ensure that air is expelled from the bottle as much as possible, and meanwhile, no air is in continuous contact with the sample through the exhaust valve. By squeezing the collection bottle 9 and sample bottle 13, a slight pressure difference can be used to cause fluid to be dispensed in the collection bottle 9 and sample bottle 13. Finally, the suspended sample is subjected to coarse filtration by the filter 10 and completely enters the sample bottle, so that simple and rapid treatment of the anaerobic microorganism sample is realized.
The above embodiments are merely for illustrating the technical solution of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (7)
1. A portable collection device of anaerobic microorganism sample, its characterized in that: comprises an outer package (1), a self-sealing sample bag (5) and an anaerobic microorganism sample collection device;
the outer package (1) is a sterile sealed transparent outer package, and the outer surface of the outer package (1) is printed with a product name (3) and a use instruction (4);
the self-sealing sample bag (5) is used for accommodating the whole collecting device;
anaerobic microorganism sample collection device includes collection bottle lid (7), collection bottle (9) and sample bottle (13), collection bottle lid (7) set up in collection bottle (9), there is discharge valve (6) at the top of collection bottle lid (7) through pipeline fixed mounting, the bottom of collection bottle lid (7) is provided with sampling spoon (8), the lateral wall fixed mounting of collection bottle (9) has connecting pipe (11) that are linked together, just the other end fixed mounting of connecting pipe (11) is at the lateral wall of sample bottle (13).
2. The portable collection device for anaerobic microbial samples according to claim 1, wherein: two ends of the outer package (1) are provided with easy-tearing structures (2).
3. The portable collection device for anaerobic microbial samples according to claim 1, wherein: the collection bottle cap (7) is of an inverted conical structure, and the collection bottle cap (7) is communicated with the collection bottle (9) through a notch.
4. The portable collection device for anaerobic microbial samples according to claim 1, wherein: the side wall of the collecting bottle (9) is provided with a net-shaped filter (10) near the bottom, and the filter (10) is communicated with the installed connecting pipe (11).
5. The portable collection device for anaerobic microbial samples according to claim 1, wherein: one end of the connecting pipe (11) is fixedly provided with a valve pipeline (12), the other end of the valve pipeline (12) is fixedly arranged on the side wall of the sample bottle (13), and the connecting pipe (11) is fixedly arranged on the side wall of the sample bottle (13) through the valve pipeline (12).
6. The portable collection device for anaerobic microbial samples according to claim 1, wherein: the collecting bottle cap (7) is fit at the top of the collecting bottle (9).
7. The portable collection device for anaerobic microbial samples according to claim 1, wherein: the collecting bottle (9) and the sample bottle (13) are elastic plastic bottles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322417064.9U CN220745930U (en) | 2023-09-06 | 2023-09-06 | Portable collection device of anaerobic microorganism sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322417064.9U CN220745930U (en) | 2023-09-06 | 2023-09-06 | Portable collection device of anaerobic microorganism sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220745930U true CN220745930U (en) | 2024-04-09 |
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ID=90553174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322417064.9U Active CN220745930U (en) | 2023-09-06 | 2023-09-06 | Portable collection device of anaerobic microorganism sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220745930U (en) |
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2023
- 2023-09-06 CN CN202322417064.9U patent/CN220745930U/en active Active
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