CN217217766U - Grape fruit stalk development detection device under tissue culture condition - Google Patents
Grape fruit stalk development detection device under tissue culture condition Download PDFInfo
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- CN217217766U CN217217766U CN202220325692.7U CN202220325692U CN217217766U CN 217217766 U CN217217766 U CN 217217766U CN 202220325692 U CN202220325692 U CN 202220325692U CN 217217766 U CN217217766 U CN 217217766U
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Abstract
The utility model discloses a grape stalk development detection device under the condition of tissue culture, which comprises a glass bottle and a bottle cap; a support is arranged at the upper part inside the glass bottle, two solid culture mediums are arranged on the support, a sealing film is sleeved outside the glass bottle, and a rubber ring is sleeved in the middle of the sealing film; the support is a support film or a support net. The utility model relates to a grape fruit stalk development detection device under tissue culture condition through setting up plastic wrap and MS culture medium, not only is favorable to making the area stalk grape to hang and cultivates, can also make things convenient for the experimenter to observe the grain condition that falls, and experimenter's experimental efficiency is higher, and the rethread sets up seal membrane and rubber circle, can ensure that the experimental environment is enough sealed, the influence of the external factor of discharge, and the experimental result after the experiment is more accurate.
Description
Technical Field
The utility model relates to a fruit stalk develops technical field, specifically is a grape fruit stalk development detection device under group banks up with earth condition.
Background
Various experiments need to be carried out in the biological cultivation process, the grape fruit stalk development is an experiment for researching which components can improve the fruit stalk tension of the grape and reducing grape falling particles, relatively, an experimenter needs a grape fruit stalk development detection device under the tissue culture condition to assist the experiment, but the development device on the current market cannot meet the experiment requirements, is not convenient enough during use, and influences the research efficiency and the research result of the experimenter.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a grape fruit stalk develops detection device under group banks up with earth condition to solve the development device on the existing market who proposes in the above-mentioned background art and can not satisfy the experimental requirement, it is convenient inadequately during the use, influence experimenter's research efficiency and research result's problem.
In order to achieve the above object, the utility model provides a following technical scheme: a grape fruit stalk development detection device under the condition of tissue culture comprises a glass bottle and a bottle cap; a support is arranged at the upper part inside the glass bottle, two solid culture mediums are arranged on the support, a sealing film is sleeved outside the glass bottle, and a rubber ring is sleeved in the middle of the sealing film; the support is a support film or a support net.
As an optimized technical proposal of the utility model, the edge of the support is connected with the bottle mouth of the glass bottle.
As a preferred technical scheme of the utility model, the solid culture medium is an MS culture medium.
As a preferred technical scheme of the utility model, the solid culture medium is filled with dichlorophenylacetic acid (2, 4-dichlorophenylacetic acid) solution with the concentration of 25 mg/L. A25 mg/L dichlorophenylacetic acid solution (25 mg/L2, 4-D) is convenient for verifying which component can improve the fruit stalk tension, reduce falling particles and is beneficial to the whole experiment.
As an optimized technical scheme of the utility model, the glass bottle is transparent visual bottle, the bottle lid is transparent visual lid. The transparent visual bottle and the transparent visual cover are convenient for experimenters to observe experimental conditions in the glass bottle, and are more convenient during experiments.
As a preferred technical scheme of the utility model, two the solid medium size and shape are the same. The two solid culture media are the same in size and shape, so that the stemmed grapes can be cultured in a suspension mode, experimental errors are not prone to occurring, and experimental results are accurate.
The peduncle grapes (which can be single-grain or multi-grain grapes) are suspended below the support, and the peduncle grapes penetrate through the support and are inserted into the solid culture medium from two ends respectively. Different components are added into the solid culture medium, so that the influence of the different components on the fruit stem tension is detected, and the falling particles are reduced. The concentration of the components in the solid culture medium is adjusted, and the influence of different concentrations on the fruit stalk tension and falling particles can be tested.
Compared with the prior art, the beneficial effects of the utility model are that: through setting up supporter and solid medium, not only be favorable to making the peduncle grape suspension culture, can also make things convenient for the experimenter to observe the falling grain condition, the experimenter's experimental efficiency is higher, and the rethread sets up seal membrane and rubber circle, can ensure that experimental environment is enough sealed, the influence of the external factor of discharge, and the experimental result after the experiment is more accurate.
Drawings
FIG. 1 is a schematic view of the present invention;
FIG. 2 is a schematic view of a peduncled grape structure according to the present invention;
FIG. 3 is a schematic top view of the MS culture medium of the present invention;
fig. 4 is a schematic diagram of an experimental result of the present invention.
In the figure: 1. a sealing film; 2. a support; 3. a solid culture medium; 4. grapes with stems; 5. a glass bottle; 6. a bottle cap; 7. a rubber ring.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Referring to fig. 1-4, the utility model provides a grape stem development detection device under the condition of tissue culture, which comprises a glass bottle (5) and a bottle cap (6); the upper part inside the glass bottle (5) is provided with a support (2), the support (2) is provided with two solid culture mediums (3), the outer side of the glass bottle (5) is sleeved with a sealing film (1), and the middle part of the sealing film (1) is sleeved with a rubber ring (7); the support (2) is a support film or a support net. The support (2) is a preservative film, and the solid culture medium is an MS culture medium; the single grape with the stem is suspended below the preservative film, the stem with the stem is respectively inserted into the MS culture medium through the preservative film and the two ends of the stem, and the edge of the preservative film is fixed at the bottle mouth of the glass bottle.
Bottle lid 6 is convenient for seal glass bottle 5, and two MS culture mediums 3 are not only convenient for place to plastic wrap 2, can also keep fresh to two MS culture mediums, and two MS culture mediums 3 are convenient for experiment to stem grape 4, and seal membrane 1 is convenient for all seal glass bottle 5 and bottle lid 6, and rubber circle 7 is convenient for fasten seal membrane 1, the external factor of discharge to influence the experimental result.
The two MS culture media 3 are the same in size and shape, so that the stemmed grapes 4 can be cultured in a hanging mode, experiment errors are not prone to occurring, and the experiment results are more accurate.
The working principle of the device is that the bottle cap 6 is convenient to seal the glass bottle 5, the preservative film 2 is convenient to place the MS culture medium 3, the MS culture medium 3 is convenient to test the grapes with stems, the sealing film 1 is convenient to seal the glass bottle 5 and the bottle cap 6, the rubber ring 7 is convenient to fasten the sealing film 1 and discharge external factors, thereby influencing the test result, the transparent visual bottle and the transparent visual cap are convenient for the experimenter to observe the test condition in the glass bottle 5, the test is more convenient, the whole device is not only beneficial to hanging and culturing the grapes with stems by arranging the preservative film 2 and the MS culture medium 3, but also is convenient for the experimenter to observe the falling particles condition, the experimental efficiency of the experimenter is higher, and the sealing film 1 and the rubber ring 7 are arranged, thereby ensuring that the experimental environment is sealed enough and discharging the influence of the external factors, the experimental result after the experiment is more accurate.
Experimental groups: both MS media 3 were filled with a 25mg/L solution of dichlorophenylacetic acid, a 25mg/L solution of dichlorophenylacetic acid (25mg/L of 2, 4-dichlorophenylacetic acid). Blank control group: no other ingredients were added to both MS media 3. After 6 days of culture, the stalks of grapes with stalks are subjected to a tension test by using a tension meter, and the test result is shown in figure 4, which shows that 2, 4-dichlorophenylacetic acid can increase the tension of the stalks of the grapes and reduce the shattering of the grapes.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (5)
1. The utility model provides a grape fruit stalk development detection device under tissue culture condition, includes glass bottle (5) and bottle lid (6), its characterized in that: the upper part inside the glass bottle (5) is provided with a support (2), the support (2) is provided with two solid culture mediums (3), the outer side of the glass bottle (5) is sleeved with a sealing film (1), and the middle part of the sealing film (1) is sleeved with a rubber ring (7); the support (2) is a support film or a support net.
2. The apparatus according to claim 1, wherein the apparatus for detecting development of grape stalks under tissue culture conditions comprises: the solid culture medium (3) is MS culture medium.
3. The apparatus according to claim 1, wherein the apparatus for detecting development of grape stalks under tissue culture conditions comprises: the glass bottle (5) is a transparent visual bottle, and the bottle cap (6) is a transparent visual cap.
4. The device for detecting grape fruit stem development under the condition of tissue culture according to claim 1, wherein: the two solid media (3) are identical in size and shape.
5. The device for detecting grape fruit stem development under the condition of tissue culture according to claim 1, wherein: the glass bottle (5) is in threaded connection with the bottle cap (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220325692.7U CN217217766U (en) | 2022-02-18 | 2022-02-18 | Grape fruit stalk development detection device under tissue culture condition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220325692.7U CN217217766U (en) | 2022-02-18 | 2022-02-18 | Grape fruit stalk development detection device under tissue culture condition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217217766U true CN217217766U (en) | 2022-08-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220325692.7U Active CN217217766U (en) | 2022-02-18 | 2022-02-18 | Grape fruit stalk development detection device under tissue culture condition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217217766U (en) |
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2022
- 2022-02-18 CN CN202220325692.7U patent/CN217217766U/en active Active
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