CN220744054U - Portable living cell transportation device - Google Patents

Abstract

The application discloses a portable living cell transportation device which is of a box type structure, wherein a storage cavity is formed in the transportation device, and a pressure gauge, a carbon dioxide monitor and a temperature monitor are arranged on the transportation box; chu Naqiang is internally provided with a heating component, the bottom wall of Chu Naqiang is provided with a fixed seat, and a culture container for culturing cells is detachably arranged on the fixed seat; when in use, the air suction holes on the transport case can be usedDeflating, wherein the air is filled with 95% of air and 5% of CO through an air filling hole on the transport case ₂ The temperature of the gas in the storage cavity is raised and kept by the heating component; the transportation device provided by the application can carry various adherent and suspended stem cells and non-stem cells only by using a common cell culture bottle and a culture medium, does not need dry ice, liquid nitrogen or frozen stock solution, can avoid complex process operations such as collection, frozen stock, thawing, culture and the like, greatly simplifies the cell transportation process, reduces the transportation cost, and is safe and efficient.

Description

Portable living cell transportation device

Technical Field

The application relates to the technical field of cell transportation, in particular to a low-cost portable living cell transportation device capable of avoiding process operations such as cell collection, freezing, thawing, culture and the like.

Background

Stem cells are present in bone marrow, blood, brain, spinal cord, skin, limbus, etc., and are responsible for renewing and repairing various injured tissues and organs in the body. The damaged tissue and organ can be repaired by stem cell transplantation, and the body function of the tissue and organ can be recovered. Therefore, stem cell research has important clinical therapeutic value. However, stem cells require extremely high demands on the culture environment and the manner of preservation, such as prolonged exposure to unfavorable temperatures, humidity, oxygen and carbon dioxide concentrations, and the cells gradually lose function and activity.

At present, the transportation modes of stem cells mainly comprise the following three modes:

1. directly placing the cells into a proper culture medium for transportation;

2. placing the cells into dry ice for frozen transportation by means of frozen stock solution;

3. and (5) placing the cells into liquid nitrogen for freezing and storing and transporting the cells.

The first transportation method directly places cells in a culture medium for transportation, and takes temperature, humidity, carbon dioxide concentration and transportation time into consideration, so that too long time can lead to the change of pH value of the culture medium, temperature reduction and increase of pollution probability of long-time exposure to air caused by lack of carbon dioxide of cells, thereby reducing cell survival rate and losing functions.

The second transportation mode relies on dry ice and frozen stock solution, and the third transportation mode is liquid nitrogen and frozen stock solution, and the two transportation modes are the most common transportation modes at present. Before transportation, the cells are protected by a freezing solution, then frozen by a program cooling instrument, and finally placed into liquid nitrogen or dry ice for transportation.

Although these two modes of transportation by dry ice or liquid nitrogen can basically meet the current cell transportation requirements, they still have the following drawbacks:

1. the transportation cost is high: dry ice and liquid nitrogen are expensive substances, and special packaging and transportation modes are needed, so that the transportation cost is relatively high;

2. the transportation time is limited: both dry ice and liquid nitrogen are limited freezing media, transportation time is limited, and long-term transportation may affect cell viability and function;

3. safety risk: dry ice and liquid nitrogen are extremely low temperature substances, and if the dry ice and the liquid nitrogen are broken or leaked carelessly in the transportation process, the dry ice and the liquid nitrogen can cause harm to transportation personnel and the environment;

4. effects on cells: both dry ice and liquid nitrogen are extremely low temperature substances that can adversely affect cell viability and function, especially for certain sensitive cell types;

5. cell-dependent cryopreservation: the cell freezing solution is basically used before cell freezing transportation, and in the freezing and transportation process, cells can be influenced by various adverse factors such as temperature, pressure, vibration and the like, so that the survival rate is reduced, and even the cells die; when the frozen stock solution transports cells, the cells can be mechanically and chemically damaged in the freezing and thawing processes, so that DNA is broken and damaged, and the biological characteristics and stability of the cells are affected; the frozen stock solution is used for transporting the cells, and the cells need to be subjected to multiple operations and treatments including cell collection, frozen stock, thawing, culture and the like, and strict quality control and standardization operations are required, so that the transportation difficulty is increased.

Therefore, the current cell transportation depends on low-temperature transportation of cell frozen stock solution, and the cell transportation needs to be frozen by the frozen stock solution, and the cell transportation needs to be thawed and cultured after the cell transportation is completed. In the freezing and thawing process, the cells may be damaged mechanically and chemically, the biological characteristics and stability of the cells are affected, and the overall transportation cost is high. Therefore, a new technical solution is needed to solve the problems existing in the prior art.

Disclosure of Invention

The application provides a portable living cell transportation device for solve current living cell transportation not only with high costs, the operation is complicated, can also influence the biological characteristic and the problem of stability of cell.

In order to achieve the above object, the present application provides the following technical solutions:

the application provides a portable living cell transportation device, which comprises a transportation box, wherein a storage cavity is formed in the transportation box, and a box door for closing or opening the storage cavity is arranged on the transportation box; the side wall of the transport case is provided with an inflation assembly and an air extraction assembly, the inflation assembly comprises an inflation hole, the inflation hole is matched with an output port of the gas transmission device, and the air extraction assembly comprises an air extraction hole; the transport case is provided with a pressure gauge, a carbon dioxide monitor and a temperature monitor; a heating component is arranged in Chu Naqiang and comprises a resistance wire; one or more fixing seats are arranged on the bottom wall of Chu Naqiang, a culture container for culturing cells is arranged on the fixing seats, and the culture container is detachably connected with the fixing seats; the plurality of culture containers are stacked in sequence, two adjacent culture containers are detachably connected, and one end of the stacked plurality of culture containers is detachably connected with the fixing seat.

In the technical scheme, the culture container is a cell culture bottle, a cell culture plate or a cell culture dish; the culture container is provided with a culture medium or a culture solution.

Further, a cell taking and placing opening is formed on the culture container, an end cover is detachably connected to the cell taking and placing opening, an air inlet hole is formed in the end cover, and Chu Naqiang is in air communication with the culture container through the air inlet hole.

Further, a clamping groove is formed at the bottom of the culture container, and a clamping protrusion is formed at the top of the culture container; when two culture containers are stacked up and down, the upper culture container is matched and inserted with the clamping protrusion at the top of the lower culture container through the clamping groove at the bottom of the upper culture container.

Further, the clamping protrusion is a cylindrical protrusion or a truncated cone-shaped protrusion protruding outwards from the top of the culture container, and the clamping groove is a cylindrical groove or a truncated cone-shaped groove matched with the clamping protrusion.

Further, a fixing groove is formed in the fixing seat, the shape of the fixing groove is the same as that of the culture container, and the size of the fixing groove is larger than that of the culture container.

Further, a damping positioning mechanism is arranged on the side wall of the fixed groove and comprises a spring and a positioning plate arranged at one end of the spring, and the other end of the spring is arranged on the side wall of the fixed groove; or, a channel allowing the spring to reciprocate is arranged on the fixing seat, the channel penetrates through the side wall of the fixing groove and is communicated with the fixing groove, and the other end of the spring is connected with the end part of the channel; when the culture container is installed in the fixed slot, the locating plate is abutted with the outer wall of the culture container, and the cultured container is extruded towards the direction of the spring, so that the spring is compressed and limits the culture container through the locating plate.

Further, the fixed slot is a quadrilateral slot, and the four side walls of the fixed slot are all provided with damping positioning mechanisms, and the four positioning plates enclose a clamping space for positioning and clamping the culture container.

Further, an installation seat for installing a gas transmission device is formed on the outer wall of the transport case, and the gas transmission device is detachably connected with the installation seat; the gas transmission device is a mixed gas cylinder; the gas transmission device stores mixed gas which is 95% of air and 5% of CO2.

Further, an electronic element chamber is arranged on the transport case, a power supply and a controller are arranged in the electronic element chamber, the power supply is electrically connected with a resistance wire, and the resistance wire is used for heating gas in the storage chamber; the pressure gauge, the carbon dioxide monitor and the temperature monitor are respectively connected with the controller through signals.

Further, the heating assembly further comprises a water bath tank, the resistance wire is used for heating water in the water bath tank, and the temperature of the gas in the storage chamber is raised through hot water in the water bath tank; the water bath is provided with a cover in an adapting way, and the cover is provided with ventilation holes.

Compared with the prior art, the application has the following beneficial effects:

the application provides a portable living cell transportation device which is of a box type structure, wherein a storage cavity is formed in the transportation device, and a pressure gauge, a carbon dioxide monitor and a temperature monitor are arranged on the transportation box; a heating component is arranged in Chu Naqiang, a fixed seat is arranged on the bottom wall of Chu Naqiang, and a culture container for culturing cells is detachably arranged on the fixed seat; when the device is used, mixed gas consisting of 95% of air and 5% of CO2 is filled through the air suction Kong Fangqi on the transport box, the temperature of the gas in the storage cavity is raised and kept by the heating component, and the transport conditions in the transport box are monitored in real time by the pressure gauge, the carbon dioxide monitor and the temperature monitor; therefore, the transportation device provided by the application can carry various adherent and suspended stem cells and non-stem cells only by using a common cell culture bottle and a culture medium, dry ice, liquid nitrogen and frozen stock solution are not needed, and the transportation mode can avoid complex process operations such as collection, frozen stock, thawing, culture and the like, so that the cell transportation process is greatly simplified, the transportation cost is reduced, the transportation device is safe and efficient, and the influence on the biological characteristics and stability of cells in the freezing and thawing processes is solved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. It should be understood that the specific shape and configuration shown in the drawings should not be considered in general as limiting upon the practice of the present application; for example, based on the technical concepts and exemplary drawings disclosed herein, those skilled in the art have the ability to easily make conventional adjustments or further optimizations for the add/subtract/assign division, specific shapes, positional relationships, connection modes, dimensional scaling relationships, etc. of certain units (components).

FIG. 1 is a schematic perspective view of a transport case according to an embodiment of the present disclosure, in which a door of the transport case is opened, a monitoring instrument such as a pressure gauge is not shown, and a plurality of stacked culture containers are disposed on a fixing seat in the transport case;

FIG. 2 is a schematic perspective view of a transport case according to an embodiment of the present application, mainly showing a fixing seat and a water bath inside the transport case;

FIG. 3 is a schematic view showing a fixing principle of a fixing base in a transport case according to an embodiment of the present disclosure for clamping and fixing a culture container by a shock absorbing and positioning mechanism;

FIG. 4 is a schematic view showing the structure of a culture vessel used in the present application in one embodiment;

FIG. 5 is a schematic view showing a structure in which a plurality of culture vessels shown in FIG. 4 are stacked one on top of another.

Reference numerals illustrate:

1. a transport case; 2. chu Naqiang; 3. a door; 4. an air filling hole; 5. an air suction hole; 6. a fixing seat; 7. a fixing groove; 8. a spring; 9. a positioning plate; 10. a culture vessel; 11. a water bath; 12. a cover; 13. ventilation holes; 14. and (5) a mounting seat.

Detailed Description

The present application is further described in detail below with reference to the attached drawings.

In the description of the present application: unless otherwise indicated, the meaning of "a plurality" is two or more. The terms "first," "second," "third," and the like in this application are intended to distinguish between the referenced objects without a special meaning in terms of technical connotation (e.g., should not be construed as emphasis on degree or order of importance, etc.). The expressions "comprising", "including", "having", etc. also mean "not limited to" (certain units, components, materials, steps, etc.).

The terms such as "upper", "lower", "left", "right", "middle", and the like, as referred to in this application, are generally used for convenience in visual understanding with reference to the drawings, and are not intended to be an absolute limitation of the positional relationship in actual products. Such changes in relative positional relationship are considered to be within the scope of the present description without departing from the technical concepts disclosed herein.

Example 1

In order to solve the problem that living cell transportation relies on dry ice, liquid nitrogen, cell cryopreservation liquid and the like in the prior art, so that transportation cost is high, and the influence of freezing and thawing processes on cell biological characteristics and stability is caused, the application provides a portable living cell transportation device which is simple in structure and low in cost, transportation cost and transportation difficulty can be effectively reduced, freezing and thawing operations are avoided, dry ice, liquid nitrogen and cell cryopreservation liquid are not needed, the influence of freezing and thawing processes on cell biological characteristics and stability is avoided, and cell activity and quality are effectively guaranteed. The structure of the portable living cell transporter will be described in detail.

The portable living cell transportation device that this application provided is box formula structure, and the most important functional part is transport case 1, see fig. 1, and transport case 1 is interior to set up and store up and hold chamber 2, stores up and holds chamber 2 and be used for placing culture vessel 10, and provides suitable condition for the cell in the culture vessel 10. Naturally, the transport case 1 is provided with a case door 3, and in order to realize sealing, the case door 3 is provided with sealing structures such as sealing strips.

In order to realize inflation and deflation, in this embodiment, an inflation assembly is arranged on the box body of the transport box 1, preferably, the inflation assembly is a pressing type air tap arranged on the side wall of the box body, the structure of the pressing type air tap can be seen from the pressing type air tap disclosed in the chinese patent No. CN201620434212.5, the air tap is a gas valve, and the gas valve comprises a pressing cover, a base, a rotor and a locking structure; the pressing cover is provided with a pressing piece matched with the rotor; the base is a hollow shell with two open ends; the pressing cover is arranged at one end of the base, and the rotor is arranged in the base; the locking structure is arranged between the pressing cover and the base and is used for locking the pressing cover on the base so as to prevent the pressing cover from moving towards the rotor. When a user needs to inflate, the pressing cover is pressed downwards to open the pressing type air tap, and then the inflatable product is inflated through the air transmission device. When the inflatable product is inflated, the pressing cover is pressed downwards again to close the pressing type air tap, and the pressing cover is locked on the base by the locking structure. Therefore, the pressing type air tap can be opened after the pressing cover is pressed down, at this time, the air tap can be used as the air pumping hole 5, and the air in the transport case 1 can be put into the air tap, and the air pumping process can be assisted by auxiliary devices such as an air pumping cylinder. After the air is pumped out, the air tap is used as an air charging hole 4 to be connected with an air transmission device for charging the storage cavity 2.

In order to realize the monitoring of the environment in the transport case 1, the transport case 1 is provided with a pressure gauge, a carbon dioxide monitor and a temperature monitor, wherein the pressure gauge, the gas concentration monitor, the temperature monitor and the like can be simple pressure gauges, gas concentration monitors, temperature monitors and the like, and can also be pressure sensors, carbon dioxide concentration monitoring sensors, temperature sensors and the like which are more data-based and informationized. Different instrument types correspond to different equipment manufacturing costs, and corresponding selection can be performed according to the requirements of users during production and manufacturing.

Of course, the sensor instrument is more convenient than the common instrument in monitoring use, and the sensor instrument can be connected with the controller to realize digital and informationized monitoring.

Some cells are relatively sensitive to temperature, so that the transport case 1 provided by the application is also provided with a heating component, and when necessary, the heating component can be matched to provide proper temperature conditions.

In this embodiment, the heating assembly includes a resistance wire, and in order to heat the resistance wire, naturally, an electronic component chamber is required to be disposed on the transport case 1, and a battery, an electric wire, and the like are installed therein. The heating assembly in this embodiment may further include a water bath 11, see fig. 1 and 2, where the resistance wire is used to heat water in the water bath 11, and heat the gas in the storage chamber through the hot water in the water bath 11, and the water bath heats slowly but the spatial temperature is uniformly distributed. In order to prevent liquid from splashing during transportation, a cover 12 can be arranged on the water bath 11 in an adapting way, and ventilation holes 13 are formed in the cover 12, so that the height of the water bath 11 can be increased appropriately.

In order to realize the monitoring of the liquid level in the water bath 11, a liquid level meter for detecting the liquid level in the water bath 11 can be adapted on the transport case 1.

In this embodiment, the electronic component chamber may further be provided with a controller, and the resistance wire, the pressure gauge, the carbon dioxide monitor and the temperature monitor are respectively connected with the controller through signals, and the constant-temperature water bath may be realized through the controller. Specifically, the water is heated to a target temperature set in the controller by heating the resistance wire, and then is kept warm. Because the temperature can naturally decrease, the heating is further needed to be continued, so that the temperature in the storage cavity 2 reaches the target temperature, namely, the temperature in the storage cavity 2 is monitored in real time through the temperature monitor, and when the controller monitors that the temperature in the storage cavity 2 is lower than the target temperature, the resistance wire is controlled to be electrified, so that the temperature in the storage cavity 2 is increased. That is, the transport case 1 provided by the application can control the on-off of the resistance wire in turn through the gas temperature in the case, so as to realize constant temperature water bath.

To fix the culture container 10, in this embodiment, one or more fixing seats 6 are provided on the bottom wall of the storage chamber 2, and the culture container 10 for culturing cells is mounted on the fixing seat 6, and the culture container 10 is detachably connected to the fixing seat 6, as shown in fig. 1.

In order to improve the space utilization, a plurality of culture containers 10 may be stacked in sequence, and two adjacent culture containers 10 may be detachably connected, i.e., a user selects to stack several culture containers 10 according to the needs. When mounted, one end of the stacked plurality of culture containers 10 is detachably connected to the fixing base 6 as shown in FIG. 5.

The culture vessel 10 may be any one or more of a cell culture flask, a cell culture plate, or a cell culture dish, and of course, may be any nonstandard product that can be used to culture cells. Cell culture flasks, plates, or dishes as used herein are commercially available.

Of course, depending on the cell culture requirements, a medium or culture broth may be provided in culture vessel 10 to provide the cells with nutrients required during transport.

In one embodiment, the culture container 10 used in the application is provided with a cell taking and placing port, an end cover is detachably connected to the cell taking and placing port, an air inlet hole is formed in the end cover, and Chu Naqiang 2 is in gas communication with the culture container 10 through the air inlet hole. Culture vessel 10 meeting such use requirements is commercially available, such as an existing cell culture flask.

To achieve stable stacking of a plurality of culture vessels 10 up and down. In this embodiment, a clamping groove may be provided at the bottom of the culture container 10, and a clamping protrusion may be provided at the top of the culture container 10, as shown in FIG. 4.

When two culture containers 10 are stacked one on top of the other, the upper culture container 10 is inserted and connected with the clamping protrusion at the top of the lower culture container 10 through the clamping groove at the bottom of the upper culture container 10, as shown in FIG. 5.

In this embodiment, the clamping protrusion is a cylindrical protrusion or a truncated cone-shaped protrusion protruding outwards from the top of the culture container 10, and the clamping groove is a cylindrical groove or a truncated cone-shaped groove adapted to the clamping protrusion. Of course, the plug-in type socket can also be a convex clip and a groove clip with other shapes, and can realize convenient plug-in.

In order to achieve a detachable connection of the holder 6 to the culture vessel 10. In this embodiment, the fixing base 6 is formed with a fixing groove 7, the shape of the fixing groove 7 is the same as that of the culture container 10, the size of the fixing groove 7 is larger than that of the culture container 10, and a certain buffer space can be reserved for the culture container 10 due to the larger size of the fixing groove 7, as shown in fig. 3. For example, a shock absorbing positioning mechanism may be provided on the side wall of the fixing groove 7.

In this embodiment, referring to fig. 3, the shock absorbing positioning mechanism includes a spring 8 and a positioning plate 9 provided at one end of the spring 8, and the other end of the spring 8 is mounted on the side wall of the fixing groove 7. Preferably, as shown in fig. 3, a channel allowing the spring 8 to reciprocate is arranged on the fixed seat 6, the channel penetrates through the side wall of the fixed groove 7 and is communicated with the fixed groove 7, and the other end of the spring 8 is connected with the end part of the channel; when the culture container 10 is mounted in the fixing groove 7, the positioning plate 9 is abutted against the outer wall of the culture container 10, and the cultured container 10 is pressed towards the spring 8, so that the spring 8 is compressed and the culture container 10 is limited by the positioning plate 9.

In a specific embodiment, with continued reference to fig. 3, the fixing groove 7 may be a quadrangular groove, and four side walls of the fixing groove 7 are respectively provided with a shock absorbing positioning mechanism, and four positioning plates 9 enclose a square clamping space for positioning and clamping the culture container 10.

The outer wall of the transport case 1 provided by the application is provided with a mounting seat 14 for mounting a gas transmission device, and the gas transmission device is detachably connected with the mounting seat 14.

In this embodiment, the mounting seat 14 may be a mounting groove formed on a side wall of the case, and a clip for clamping the air delivery device may be disposed in the mounting groove, and the shape of the clip may be set in a self-defined manner according to the shape of the air delivery device.

Of course, the outer wall of the transport case 1 may be provided with a hanging member, the gas transmission device may be provided with a corresponding connecting member, and the hanging member and the connecting member may be correspondingly detached and installed. For example, the hanging piece is a clamping ring, and the connecting piece is a clamping hook.

In this embodiment, the gas delivery device may be a mixed gas cylinder; the mixed gas cylinder stores mixed gas which is 95% of air and 5% of CO2. The aeration holes 4 are aerated by the mixed gas cylinder.

The portable living cell transportation device is provided with a pressure gauge, a carbon dioxide monitor and a temperature monitor; a heating component is arranged in the storage cavity 2, a fixed seat 6 is arranged on the bottom wall of the storage cavity 2, and a culture container 10 for culturing cells is detachably arranged on the fixed seat 6; during use, the air can be discharged through the air exhaust hole 5 on the transport case 1, mixed gas consisting of 95% of air and 5% of CO2 is filled through the air charging hole 4 on the transport case 1, the temperature of the gas in the storage cavity 2 is increased through the heating component, the temperature is kept, and the transport condition in the transport case 1 is monitored in real time through the pressure gauge, the carbon dioxide monitor and the temperature monitor. The transportation device provided by the application can carry various adherent and suspended stem cells and non-stem cells only by using a common cell culture bottle and a culture medium, dry ice, liquid nitrogen and frozen stock solution are not needed, the transportation mode can avoid complex process operations such as collection, frozen storage, thawing, culture and the like, the cell transportation process is greatly simplified, the transportation cost is reduced, the transportation device is safe and efficient, and the influence on the biological characteristics and stability of cells in the freezing and thawing processes is solved.

Example two

The present embodiment provides a portable living cell transporting device, which is different from the portable living cell transporting device provided in the first embodiment in that: the inflation assembly and the air extraction assembly are arranged on the side wall of the transport case 1 as two parts, the inflation assembly is used for inflation, and the air extraction assembly is used for deflation.

In this embodiment, the subassembly of aerifing is including setting up the charging connector on transport case 1, forms on the charging connector and aerifys hole 4, and the one end that the charging connector is located transport case 1 is provided with the rubber pad, and when aerifing hole 4 and the output port of gas-supply device link to each other, and gas-supply device is aerifyd to aerifing hole 4, and the rubber pad is opened under the effect of pressure, allows gaseous input, and when gas-supply device stopped to aerifing, the rubber pad was closed under the gas pressure in transport case 1, realizes the seal to aerifing hole 4.

In this embodiment, the gas delivery device is a mixed gas cylinder.

In another embodiment, the output end of the mixed gas cylinder is connected with a pressure pipeline, and a one-way valve and a delivery pump are arranged on the pressure pipeline, so that gas delivery and one-way gas circulation are further ensured.

In the embodiment, the air extraction component can be arranged as an air outlet port arranged on the side wall of the transport case 1, the air outlet port is adaptively connected with a sealing cover, and a sealing ring is arranged in the sealing cover; when the air is required to be discharged, the sealing cover can be opened, and the sealing cover can be screwed up after the air discharge is completed.

Example III

The present embodiment provides a portable living cell transporting device, which is different from the portable living cell transporting device provided in the first embodiment in that: the heating element who sets up in storing chamber 2 is the resistance wire, is provided with the electronic component cavity on the transport case 1, is provided with the power in the electronic component cavity, and the power is connected with the resistance wire electricity, directly heats up the indoor gas of storing through the resistance wire, and this kind of heating up mode is faster than the water bath intensification, but leads to each partial temperature inhomogeneous in the space easily.

In order to achieve uniform gas temperature, an electric fan may be disposed in the storage chamber 2 to agitate the gas in the storage chamber 2 to uniform the temperature.

A handle can also be provided on the transport case 1 for portability.

Example IV

Based on the portable living cell transportation device provided above, the application also provides a use method of the portable living cell transportation device, which at least comprises the following steps:

s1: mounting the culture container 10 for target transportation on the fixed seat 6, and closing the box door 3;

s2: discharging the gas in the transport case 1 through the gas-discharging hole 5, and charging mixed gas consisting of 95% of air and 5% of CO2 into the transport case 1;

s3: heating and preserving the temperature of the mixed gas in the storage cavity 2 by using a heating component so as to maintain the temperature in the storage cavity 2 at a target temperature;

s4: when the carbon dioxide concentration in the transport case 1 is reduced to half of the carbon dioxide concentration in the initial mixed gas, the gas in the transport case 1 is discharged through the gas-discharging hole 5, and then the mixed gas composed of 95% air and 5% CO2 is filled into the transport case 1, and the step S3 is repeated.

In step S2, the aeration is terminated when the pressure in the transport box 1 and the carbon dioxide reach the requirements, and then the temperature is continuously monitored.

In step S4, when the carbon dioxide concentration is reduced, the gas inside may be discharged through the gas-discharging hole 5, and then the mixed gas may be charged again. For convenience, a special small portable gas mixing tank, i.e. a gas mixing cylinder, may be provided for the gas filling holes 4. Since the consumption of carbon dioxide by cells results in a decrease in the concentration of carbon dioxide, the supplementary mixed gas is considered, and as for ventilation when the consumption of carbon dioxide is half, the temperature change due to the total discharge of the gas is mainly considered, and thus half ventilation is considered.

In order to verify that the portable living cell transportation device provided in the present application can actually carry out cell transportation, the inventors carried out a verification experiment as follows:

the inventors put an incubator containing mesenchymal stem cells in a vacuum bag, then put a mixed gas of 95% air and 5% CO2 therein, and then put the incubator in a 37 ℃ incubator for culturing, and initially detect that the pH value of the cell culture medium is about 7.4. The inventors measured the pH value of the cell supernatant at 12h, 24h and 36h, respectively, of the cell placed in a vacuum bag filled with a mixed gas, and found that the pH value of the cell supernatant did not change within 24h, and the optimum pH value of the cell was in the range of 7.35 to 7.45. When the pH was measured to be 7.3 at 36 hours upwards, then the cell state was observed with a microscope, which was not different from the cells normally cultured in the cell incubator.

Therefore, the verification experiment proves that the portable living cell transportation device provided by the application can effectively and portably transport cells for long distances, and the cells in the device can be stored for a long time.

In summary, the portable living cell transportation device and the use method thereof provided by the application solve the defect of traditional cell transportation, and the transportation device provided by the application can carry various adherent and suspended stem cells and non-stem cells only by using a common cell culture bottle and a culture medium, and the transportation method can avoid complex process operations such as collection, freezing, thawing, culture and the like, thereby greatly simplifying the cell transportation process, reducing the transportation cost, and being safe and efficient.

The portable living cell transportation device reduces the influence of temperature, pH value and the like on cells in the transportation process; the problems that the dry ice and the liquid nitrogen are not used, the damage to transportation personnel and the environment is possibly caused, the volatilization timeliness of the dry ice and the liquid nitrogen is short and the like due to careless breakage or leakage in the transportation process are solved; the frozen stock solution is not used in transportation, so that the problems of cell collection, frozen stock, thawing, culture and the like before and after transportation are solved.

Any combination of the technical features of the above embodiments may be performed (as long as there is no contradiction between the combination of the technical features), and for brevity of description, all of the possible combinations of the technical features of the above embodiments are not described; these examples, which are not explicitly written, should also be considered as being within the scope of the present description.

The foregoing has outlined and detailed description of the present application in terms of the general description and embodiments. It should be appreciated that numerous conventional modifications and further innovations may be made to these specific embodiments, based on the technical concepts of the present application; but such conventional modifications and further innovations may be made without departing from the technical spirit of the present application, and such conventional modifications and further innovations are also intended to fall within the scope of the claims of the present application.

Claims (9)

1. The portable living cell transportation device is characterized by comprising a transportation box, wherein a storage cavity is formed in the transportation box, and a box door for closing or opening the Chu Naqiang is arranged on the transportation box; the side wall of the transport case is provided with an inflation assembly and an air extraction assembly, the inflation assembly comprises an inflation hole, the inflation hole is matched with an output port of the air transmission device, and the air extraction assembly comprises an air extraction hole; the transport case is provided with a pressure gauge, a carbon dioxide monitor and a temperature monitor; a heating assembly is arranged in the Chu Naqiang, and the heating assembly comprises a resistance wire; one or more fixing seats are arranged on the bottom wall of the Chu Naqiang, a culture container for culturing cells is arranged on the fixing seats, and the culture container is detachably connected with the fixing seats; a plurality of culture containers are stacked in sequence, two adjacent culture containers are detachably connected, and one end of the stacked culture containers is detachably connected with the fixing seat.

2. The portable living cell transportation device according to claim 1, wherein the culture container is a cell culture flask, a cell culture plate, or a cell culture dish;

a culture medium or a culture solution is arranged in the culture container;

the cell picking and placing port is formed on the culture container, an end cover is detachably connected to the cell picking and placing port, an air inlet hole is formed in the end cover, and Chu Naqiang is in gas communication with the culture container through the air inlet hole.

3. The portable living cell transportation device according to claim 1 or 2, wherein a clamping groove is formed at a bottom of the culture container, and a clamping protrusion is formed at a top of the culture container; when two culture containers are stacked up and down, the upper culture container is matched and inserted with the clamping protrusion at the top of the lower culture container through the clamping groove at the bottom of the upper culture container.

4. The portable living cell transporting device according to claim 3, wherein the engaging protrusion is a cylindrical protrusion or a truncated cone-shaped protrusion protruding outward from the top of the culture container, and the engaging groove is a cylindrical groove or a truncated cone-shaped groove adapted to the engaging protrusion.

5. The portable living cell transporting device according to claim 3, wherein a fixing groove is formed in the fixing base, the shape of the fixing groove is identical to that of the culture container, and the size of the fixing groove is larger than that of the culture container;

the side wall of the fixed groove is provided with a damping positioning mechanism, the damping positioning mechanism comprises a spring and a positioning plate arranged at one end of the spring, and the other end of the spring is arranged on the side wall of the fixed groove; or alternatively, the first and second heat exchangers may be,

the fixing seat is provided with a channel allowing the spring to reciprocate, the channel penetrates through the side wall of the fixing groove and is communicated with the fixing groove, and the other end of the spring is connected with the end part of the channel;

when the culture container is installed in the fixed slot, the locating plate is abutted with the outer wall of the culture container, and the cultured container is extruded towards the direction of the spring, so that the spring is compressed and limits the culture container through the locating plate.

6. The portable living cell transporting device according to claim 5, wherein the fixing groove is a quadrangular groove, the four side walls of the fixing groove are provided with the damping positioning mechanisms, and the four positioning plates enclose a clamping space for positioning the culture container.

7. The portable living cell transportation device according to claim 1, wherein a mounting seat for mounting a gas transmission device is formed on an outer wall of the transportation box, and the gas transmission device is detachably connected with the mounting seat; the gas transmission device is a mixed gas cylinder; and the gas transmission device stores mixed gas.

8. The portable living cell transportation device according to claim 1, wherein an electronic component chamber is provided on the transportation box, a power supply and a controller are provided in the electronic component chamber, the power supply is electrically connected with the resistance wire, and the resistance wire is used for heating the gas in the storage chamber; the pressure gauge, the carbon dioxide monitor and the temperature monitor are respectively connected with the controller through signals.

9. The portable living cell transportation device according to claim 1, wherein the heating assembly further comprises a water bath, the resistance wire is used for heating water in the water bath, and the heating of the gas in the storage chamber is performed by the hot water in the water bath;

the water bath is provided with a cover in an adapting way, and the cover is provided with ventilation holes.