CN111322807A - Liquid nitrogen-free program cooling instrument - Google Patents
Liquid nitrogen-free program cooling instrument Download PDFInfo
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- CN111322807A CN111322807A CN202010334244.9A CN202010334244A CN111322807A CN 111322807 A CN111322807 A CN 111322807A CN 202010334244 A CN202010334244 A CN 202010334244A CN 111322807 A CN111322807 A CN 111322807A
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Images
Classifications
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D11/00—Self-contained movable devices, e.g. domestic refrigerators
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
- F25B9/00—Compression machines, plants or systems, in which the refrigerant is air or other gas of low boiling point
- F25B9/002—Compression machines, plants or systems, in which the refrigerant is air or other gas of low boiling point characterised by the refrigerant
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
- F25B9/00—Compression machines, plants or systems, in which the refrigerant is air or other gas of low boiling point
- F25B9/14—Compression machines, plants or systems, in which the refrigerant is air or other gas of low boiling point characterised by the cycle used, e.g. Stirling cycle
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D19/00—Arrangement or mounting of refrigeration units with respect to devices or objects to be refrigerated, e.g. infrared detectors
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D23/00—General constructional features
- F25D23/08—Parts formed wholly or mainly of plastics materials
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D29/00—Arrangement or mounting of control or safety devices
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D2400/00—General features of, or devices for refrigerators, cold rooms, ice-boxes, or for cooling or freezing apparatus not covered by any other subclass
- F25D2400/36—Visual displays
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- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Mechanical Engineering (AREA)
- Thermal Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Combustion & Propulsion (AREA)
- Devices That Are Associated With Refrigeration Equipment (AREA)
Abstract
The invention provides a liquid nitrogen-free program cooling instrument. The invention comprises a freezing storage box for storing a biological sample, a refrigeration structure for cooling the interior of the freezing storage box, and a control structure for monitoring the temperature of the interior of the freezing storage box and controlling the output power of the refrigeration structure, wherein the freezing storage box comprises a heat insulation structure and a cold guide structure, the heat insulation structure comprises a lower foaming box and an upper foaming box combined with the lower foaming box, a cell sample end temperature sensor is arranged in the heat insulation structure, the cold guide structure is arranged in the lower foaming box and used for transmitting cold for a container for placing the biological sample, a temperature regulation structure is arranged on the lower surface of the cold guide block, the refrigeration structure comprises a Stirling refrigerator, and a cold head of the Stirling refrigerator extends into the lower foaming box and then is connected with the cold guide block. The invention applies Stirling refrigeration cycle, overcomes the defects of slow cell freezing time and low cell survival rate of the freezing box, and avoids the dependence of a program temperature reducer on liquid nitrogen in the traditional technology.
Description
Technical Field
The invention relates to the field of biological medical treatment, in particular to a liquid nitrogen-free program cooling instrument.
Background
When the biological sample is stored at low temperature, the water form in the organism is in a range of 0 ℃ to-60 ℃, hexagonal ice crystals are formed, the life activity in cells is more active in the temperature range, and the hexagonal ice crystals can seriously damage cell membranes and organelles. When the temperature reaches the range of-60 ℃ to-80 ℃, the vital activity in the cells is obviously reduced in the range, the cells can form square ice blocks, and the damage to cell membranes and organelles is weakened, so that people can store the living cells in the ultra-low temperature refrigerator. However, the biochemical activity of the cells still proceeds at such temperatures, which is a constant aging process for the cells, and thus the storage of living cells under such temperature conditions is not recommended. As the temperature is lowered, the biochemical reaction activity in the cells approaches the process of stopping when the temperature is lowered to-130 ℃, and the temperature is the optimum temperature for living cells and tissues to store.
The reagent and the sample such as urine tissue for extracting biomacromolecules can be safely stored for a long time as the proper temperature is provided. For living cells, in addition to the prevailing temperature, i.e.below-130 ℃, a suitable cooling process is required.
For living cells, if the temperature reduction speed is too fast, water in the living cells cannot penetrate into the outside, so hexagonal ice crystals can be formed in the cells, cell membranes can be damaged, organelles can be damaged, and the cells can die. If the temperature of the cells is too slow, osmotic pressure is generated inside and outside the cell walls in the slow temperature reduction process, so that the cells are dehydrated, and the concentration of salt in the cells is increased, so that the cells are damaged. Therefore, for the cell activity, before the cells are put into a liquid nitrogen tank for safe storage, different stages of proper temperature reduction are provided, and different temperature reduction rates are adopted, which is very important. Therefore, the cell injury can be effectively reduced, and the recovery rate is improved.
The currently applied program cooling is divided into a program cooling box, a program cooling instrument of a liquid nitrogen injection type and a lifting type cooling instrument utilizing the temperature gradient of a gas phase region in a liquid nitrogen container. The three cooling instruments are widely applied, and simultaneously, the respective weaknesses are exposed.
Firstly, the programmed cooling box is only suitable for some cells or tissues insensitive to temperature, the freezing time of the cells is slow, the cooling rate is uncontrollable, generally 3-4 hours, the cells are placed in a liquid nitrogen tank for safe storage, and the cell survival rate is very low;
secondly, the program cooling instrument of the liquid nitrogen injection type has solved the deficiency of the cell cooling box, but the liquid nitrogen injection type cooling instrument is expensive, and the low temperature electromagnetic valve installed in the liquid nitrogen conveying pipeline of the instrument works under the condition of low temperature, water vapor in the air can dew and frost on the valve, the coil and the circuit structure of the electromagnetic valve are easy to damage, thereby causing the problems of not strictly closing the valve, discharging a large amount of nitrogen from the safety valve, high pressure indication of the pressure gauge and the like.
Finally, the general cells and cell tissues are cooled to-60 ℃ or-80 ℃ by a lifting cooling instrument, and then can directly enter a liquid nitrogen container for storage, the lifting cooling instrument is adopted, a test sample is carried out in a gas phase area in a liquid nitrogen container, so that the size and the shape of the sample are constrained by the space geometry of the liquid nitrogen container, the gas phase area in the liquid nitrogen container is communicated with the air, the temperature gradient of the gas phase area is easily influenced by the surrounding environment, and the size of the sample in the temperature gradient direction in the liquid nitrogen container cannot be too large.
Disclosure of Invention
In view of the above-mentioned technical problems, a programmed temperature-reducing instrument without liquid nitrogen is provided. The technical means adopted by the invention are as follows:
a program cooling instrument without liquid nitrogen comprises a freezing storage box for storing a biological sample, a refrigerating structure for cooling the interior of the freezing storage box, and a control structure for monitoring the temperature of the interior of the freezing storage box and controlling the output power of the refrigerating structure, wherein the freezing storage box comprises a heat insulation structure and a cold guide structure, the heat insulation structure comprises a lower foaming box and an upper foaming box combined with the lower foaming box, a cell sample end temperature sensor is arranged in the heat insulation structure, the cold guide structure is arranged in the lower foaming box and used for placing a container of the biological sample, the cold guide structure transmits cold energy to cool the sample, an inner side plate of the lower foaming body of the cold guide structure is a resin piece, a cold guide block is arranged at the upper end of the cold guide structure, a temperature adjusting structure is arranged on the lower surface of the cold guide block and used for restoring the interior of the freezing storage box to an initial temperature, and the refrigerating structure comprises a Stirling refrigerator, the cold head of the Stirling refrigerator extends into the lower foaming box and then is connected with the cold conducting block, the cold head and the lower foaming box are sealed by ultralow-temperature sealant, and the control structure is electrically connected with the Stirling refrigerator, the cell sample end temperature sensor and the temperature adjusting structure respectively.
Furthermore, the Stirling refrigerator takes helium or hydrogen as a working medium.
Furthermore, a cold end temperature sensor and a hot end temperature sensor are respectively arranged at the cold end and the hot end of the Stirling refrigerator, and the control structure is electrically connected with the cold end temperature sensor and the hot end temperature sensor respectively.
Further, the temperature regulation structure comprises an electric heating wire, and the electric heating wire is coiled at the bottom of the cold guide block.
Further, the cryopreservation research device for the biological sample, which is arranged on the cold guide structure, comprises a low-temperature bottle, a straw, a blood bag and a microporous plate.
Furthermore, the Stirling refrigerator is fixed on a left baffle and a right baffle, a certain space is reserved between the left baffle and the right baffle and the bottom end of the lower foaming box, the space is used as an air suction channel of the Stirling refrigerator, the top ends of the left baffle and the right baffle are connected with the bottom end of the lower foaming box, the bottom ends of the left baffle and the right baffle are fixed on the bottom plate, a plurality of groups of ventilation holes are formed in the left baffle and the right baffle, and one baffle is provided with a control panel fixed on the baffle.
Furthermore, a spring gasket and a rubber pad are arranged between the bottom ends of the left baffle plate and the right baffle plate and the bottom plate.
Further, lower foaming bottom of the case portion is equipped with inhales the sound structure, including the abatvoix with inhale the sound cotton.
The invention relates to a machine which realizes the circulation of a gas refrigerator by using a Stirling refrigerator, applying Stirling refrigeration circulation, taking helium or hydrogen as a working medium and applying a heat return principle in a closed system to obtain low temperature and cold. The defects that the cell freezing time of the freezing box is slow and the cell survival rate is very low are overcome; the dependence of the program cooling instrument on liquid nitrogen in the traditional technology is avoided, compared with the program cooling instrument of a liquid nitrogen injection type, the cost is lower, a liquid nitrogen conveying pipeline is not arranged in the system, the risk of the liquid nitrogen pipe in the using process is reduced, and the defects of constraint of the geometric shape of a liquid nitrogen container control in the lifting cooling instrument and the like can be avoided.
For the above reasons, the present invention can be widely applied to the field of biomedical science.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a front view of the internal structure of the present invention.
Fig. 2 is a view of the stirling cooler main engine.
Fig. 3 is a left side view of the structure fixing diagram.
Fig. 4 is a front view of the external structure.
FIG. 5 is a cross-sectional view of the internal structure of the present invention.
In the figure: 1. mounting a foaming box; 2. sending a foam box; 3. a control panel; 4. anchor bolts; 51. cooling the head; 52. an air suction opening; 53. an air outlet; 54. a hot end temperature sensor; 55. a cold end temperature sensor; 6. a rubber pad; 7. a spring washer; 8. a left baffle; 9. a right baffle; 10. an upper cover plate; 11. a microcomputer display; 12. a base plate; 13. a container; 14. and a cold conducting block.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 5, the embodiment of the invention discloses a liquid nitrogen-free program cooling instrument, which comprises a front panel of the program cooling instrument, a peripheral plate of the program cooling instrument and an upper cover plate 10 hinged to the peripheral plate of the program cooling instrument.
The internal part of the program cooling instrument front panel, the program control cooling instrument surrounding plate and the upper cover plate comprises a freezing storage box for storing biological samples, a refrigerating structure for cooling the interior of the freezing storage box, and a control structure for monitoring the temperature of the interior of the freezing storage box and controlling the output power of the refrigerating structure, wherein the freezing storage box comprises a heat insulation structure and a cooling guide structure, the heat insulation structure comprises a lower foaming box 2 and an upper foaming box 1 combined with the lower foaming box, a cell sample end temperature sensor is arranged in the heat insulation structure, the cooling guide structure is arranged in the lower foaming box and is used for placing a container 13 of the biological samples, transferring cold energy and cooling the samples, the inner side plate of the lower foaming body of the cooling guide structure is a resin piece, and the upper end of the cooling guide structure is provided with a cooling guide block 14. The resin piece has poor cold conduction performance, and can reduce the loss of cold energy; the cold conduction coefficient of the cold conduction block is high. Lead cold block lower surface and set up temperature regulation structure, temperature regulation structure is used for freezing the incasement portion of depositing and resumes to initial temperature, refrigeration structure includes the stirling refrigerator, the cold head 51 of stirling refrigerator stretches into it links to each other with the cold block to lead behind the bubble box down, adopt sealed glue of ultra-low temperature to seal between cold head and the lower bubble box down, control structure respectively with stirling refrigerator, cell sample end temperature sensor, temperature regulation structure electric connection.
In this embodiment, the cell sample end temperature sensor selects a temperature probe of PT1000 with a precision of 0.3 degrees/minute, and is disposed on the cold guide block, and the temperature of the sample is transmitted to the temperature probe through the cold guide block, so that the temperature of the cell sample is displayed on the control panel 3.
The Stirling refrigeration cycle realizes the circulation of a gas refrigerator by applying a heat return principle in a closed system to obtain low temperature and cold, and the refrigeration temperature can reach-190 ℃. The ideal working process of the Stirling refrigerator is reversible cycle consisting of two constant volume heat absorption processes and two constant temperature expansion processes, and the heat released in the constant volume heat release process is just absorbed in the constant volume heat absorption process. The heat engine absorbs heat from a high temperature heat source during a constant temperature (T1) expansion process and releases heat to a low temperature heat source during a constant temperature (T2) compression process. The application of the Stirling refrigerator on the program temperature reduction instrument ensures the uniformity of the temperature. In a preferred embodiment, the Stirling refrigerator uses helium or hydrogen as a working medium.
As shown in fig. 2, the stirling cryocooler includes an air suction port 52 and an air exhaust port 53, and in order to facilitate determining whether the stirling cryocooler is operating normally, as a preferred embodiment, a cold end temperature sensor 55 and a hot end temperature sensor 54 are respectively disposed at the cold end and the hot end of the stirling cryocooler, the control structure is electrically connected to the cold end temperature sensor and the hot end temperature sensor, and the cold end temperature sensor and the hot end temperature sensor may also be PT1000 temperature probes.
The temperature adjusting structure comprises an electric heating wire, and the electric heating wire is coiled at the bottom of the cold guide block. In order to meet the use requirement of the program temperature reduction instrument, the two functions of the temperature adjusting mechanism and the Stirling refrigerating mechanism are combined for use. When the temperature adjusting device is used specifically, the temperature of the freezing storage box at the previous time is adjusted to the initial temperature rapidly through the temperature adjusting structure in advance. When the temperature of the electric heating wire is adjusted, the Stirling refrigerator is stopped.
The upper foaming box and the lower foaming box form a heat preservation system, and the heat preservation box is made of polyurethane foam. The internal volume of the heat preservation system can be designed into different sizes according to requirements. Go up the foaming case and detain at foaming case first half section down outward, such structure is even in order to avoid between the foaming box body zonulae occludens to adopt sealed, also can be under the ultra-low temperature environment, meet the condition that the shrinkage deformation can take place for cold, and then produce and leak cold the phenomenon. The cryopreservation research device for the biological sample, which is arranged on the cold conduction structure, comprises a low-temperature bottle, a straw, a blood bag and a microporous plate. In this embodiment, the cell sap test tube is placed in the test solution tray or on the micro-porous plate, and the test solution tray or the micro-porous plate is placed on the cold guide block. The volume of the heat preservation system is enough to put down a standard microporous plate, a blood bag and a reagent tube in the test solution tray.
The Stirling refrigerator is fixed on a left baffle 8 and a right baffle 9, a certain space is reserved between the left baffle and the bottom end of the lower foaming box, the space is used as an air suction channel of the Stirling refrigerator, the top ends of the left baffle and the right baffle are connected with the bottom end of the lower foaming box, the bottom ends of the left baffle and the right baffle are fixed on a bottom plate, a plurality of groups of ventilation holes are formed in the left baffle and the right baffle, one baffle is provided with a control panel fixed on the baffle, and the bottom plate can be fixed in a space position through foundation bolts 4.
The Stirling cooler generates vibration during operation, so that as a preferred embodiment, spring washers and rubber pads are arranged between the bottom ends of the left and right baffles and the bottom plate 12, so as to reduce noise. In the operation of the Stirling cooler, the generated vibration can be attenuated through the spring gasket and the rubber pad, so that the generated resonance and noise can be greatly reduced.
In order to further reduce the noise generated at the upper end of the Stirling cooler, as a preferred embodiment, a sound absorption structure is arranged in the lower foaming box, and comprises a sound absorption wall structure consisting of sound absorption plates and a sound absorption cotton structure.
The microcomputer display 11 is embedded in the appearance panel of the program cooling instrument, is connected with the control panel and is used for displaying an experimental data curve, connecting a printer to print data or connecting a computer to store real-time electronic information and finally performing logic control operation.
In the specific implementation process of the invention, the temperature sensor at the cell sample end transmits the acquired temperature to the control system, the acquired temperature is compared with the preset temperature, and the output power of the cold end and the hot end of the Stirling refrigerant is controlled through the output end.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. A program cooling instrument without liquid nitrogen is characterized by comprising a freezing storage box for storing a biological sample, a refrigerating structure for cooling the interior of the freezing storage box, and a control structure for monitoring the temperature of the interior of the freezing storage box and controlling the output power of the refrigerating structure, wherein the freezing storage box comprises a heat insulation structure and a cooling guide structure, the heat insulation structure comprises a lower foaming box and an upper foaming box combined with the lower foaming box, a cell sample end temperature sensor is arranged in the heat insulation structure, the cooling guide structure is arranged in the lower foaming box and used for transferring cooling capacity for a container for placing the biological sample and cooling the sample, an inner side plate of the lower foaming body of the cooling guide structure is a resin piece, a cooling guide block is arranged at the upper end of the cooling guide structure, a temperature adjusting structure is arranged on the lower surface of the cooling guide block and used for restoring the interior of the freezing storage box to an initial temperature, the refrigeration structure comprises a Stirling refrigerator, a cold head of the Stirling refrigerator extends into the lower foaming box and then is connected with the cold guide block, the cold head and the lower foaming box are sealed by ultralow-temperature sealant, and the control structure is electrically connected with the Stirling refrigerator, the cell sample end temperature sensor and the temperature adjusting structure respectively.
2. The liquid nitrogen-free program cooling instrument according to claim 1, wherein the Stirling cryocooler uses helium or hydrogen as a working medium.
3. The liquid nitrogen-free program cooling instrument according to claim 1, wherein a cold end temperature sensor and a hot end temperature sensor are respectively arranged at the cold end and the hot end of the Stirling refrigerator, and the control structure is electrically connected with the cold end temperature sensor and the hot end temperature sensor respectively.
4. The programmable liquid nitrogen-free temperature reducing instrument according to claim 1, wherein the temperature regulating structure comprises an electric heating wire which is coiled at the bottom of the cold conducting block.
5. The programmable thermal cycler of claim 1, wherein the cooling structure comprises a cryovial, a pipette, a blood bag, and a microplate.
6. The liquid nitrogen-free program cooling instrument according to claim 1, wherein the stirling cryocooler is fixed to a left baffle and a right baffle, a space is reserved between the left baffle and the right baffle and the bottom end of the lower bubbling box, the space is used as an air suction channel of the stirling cryocooler, the top ends of the left baffle and the right baffle are connected with the bottom end of the lower bubbling box, the bottom ends of the left baffle and the right baffle are fixed to the bottom plate, a plurality of groups of ventilation holes are formed in the left baffle and the right baffle, and one baffle is provided with a control panel fixed to the baffle.
7. The programmed temperature-reducing instrument without liquid nitrogen as claimed in claim 1, wherein spring washers and rubber pads are provided between the bottom ends of the left and right baffle plates and the bottom plate.
8. The liquid nitrogen-free program cooling instrument as claimed in claim 1, wherein a sound absorbing structure is arranged at the bottom end of the lower foaming box, and comprises a sound absorbing plate and sound absorbing cotton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010334244.9A CN111322807A (en) | 2020-04-24 | 2020-04-24 | Liquid nitrogen-free program cooling instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010334244.9A CN111322807A (en) | 2020-04-24 | 2020-04-24 | Liquid nitrogen-free program cooling instrument |
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| Publication Number | Publication Date |
|---|---|
| CN111322807A true CN111322807A (en) | 2020-06-23 |
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|---|---|---|---|
| CN202010334244.9A Pending CN111322807A (en) | 2020-04-24 | 2020-04-24 | Liquid nitrogen-free program cooling instrument |
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| CN (1) | CN111322807A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114739075A (en) * | 2022-03-31 | 2022-07-12 | 青岛海尔生物医疗股份有限公司 | Method and device for cooling program cooling instrument and program cooling instrument |
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