CN220665322U

CN220665322U - Nucleic acid extraction detector

Info

Publication number: CN220665322U
Application number: CN202322263155.1U
Authority: CN
Inventors: 易中豪; 李烁; 盛涛; 潘良斌; 陈鑫
Original assignee: Chengdu Boao Jingxin Biotechnology Co ltd; Beijing Capitalbio Technology Co ltd; Guangzhou National Laboratory
Current assignee: Chengdu Boao Jingxin Biotechnology Co ltd; Beijing Capitalbio Technology Co ltd; Guangzhou National Laboratory
Priority date: 2023-08-22
Filing date: 2023-08-22
Publication date: 2024-03-26
Anticipated expiration: 2033-08-22

Abstract

The utility model discloses a nucleic acid extraction detector, which comprises: a nucleic acid extraction module that extracts a nucleic acid sample from the sample solution; a nucleic acid detection module for detecting a nucleic acid sample; the pipetting module comprises a pipetting part, wherein the pipetting part is used for transferring the nucleic acid sample from the access position to the filling position and injecting the nucleic acid sample into the nucleic acid detection module through the sample injection hole; and the driving module is used for driving the movement of the pipetting part. According to the nucleic acid extraction detector provided by the utility model, the nucleic acid extraction module is used for extracting the nucleic acid sample, and the driving module is used for driving the pipetting module to transfer the nucleic acid sample to the nucleic acid detection module for detection, so that the automatic operation of nucleic acid transfer in the nucleic acid detection process is realized, the efficiency of nucleic acid work is improved, the problems of diffusion pollution and disordered placement of the nucleic acid sample are reduced, and the reliability of the nucleic acid detection work is improved.

Description

Nucleic acid extraction detector

Technical Field

The utility model relates to the technical field of medical instruments, in particular to a nucleic acid extraction detector.

Background

At present, in the process of extracting and detecting nucleic acid, an operator firstly extracts nucleic acid from a sample solution through an extraction instrument, then the operator transfers the extracted nucleic acid sample to a nucleic acid detection instrument for detection, and the two instruments work independently in the whole process. However, this solution not only makes the workload of the operator great and the extraction and detection process time long, resulting in low extraction and detection efficiency, but also may have problems of diffusion pollution of the nucleic acid sample and confusion of results caused by misplacement of the nucleic acid sample due to misoperation during the process of transferring the nucleic acid sample by the operator, thereby affecting the normal operation of the nucleic acid extraction and detection work.

Therefore, how to improve the working efficiency and reliability of nucleic acid extraction detection is a technical problem that a person skilled in the art needs to solve at present.

Disclosure of Invention

In view of the above, an object of the present utility model is to provide a nucleic acid extraction detector for improving the working efficiency and reliability of nucleic acid extraction detection.

In order to achieve the above object, the present utility model provides the following technical solutions:

a nucleic acid extraction detector comprising:

a nucleic acid extraction module for extracting a nucleic acid sample from the sample solution;

a nucleic acid detection module for detecting a nucleic acid sample;

the pipetting module comprises a pipetting part, wherein the pipetting part is used for transferring the nucleic acid sample extracted by the nucleic acid extraction module from an access position to a filling position and injecting the nucleic acid sample into the nucleic acid detection module through a sample injection hole;

and the driving module is used for driving the movement of the pipetting part.

Optionally, in the nucleic acid extraction detector described above, the pipetting module further includes a first rail portion, a second rail portion, and a third rail portion;

the first track part extends along the direction from the access position to the nucleic acid detecting module to form a first direction of movement, and the second track part can move along the extending direction of the first track part;

the second rail part extends along the height direction of the nucleic acid detecting module to form a second movement direction, and the third rail part can move along the extending direction of the second rail part;

the third track part extends from the second track part to the filling position to form a third movement direction, the liquid transferring part moves along the extending direction of the third track part, and any two of the first movement direction, the second movement direction and the third movement direction are perpendicular to each other.

Optionally, in the nucleic acid extraction detector, the pipetting part includes a loading gun and an ultraviolet glue loading gun, the loading gun extracts and injects the nucleic acid sample through a loading gun head, and the loading gun head are detachably connected;

after the sample adding gun is used for injecting the nucleic acid sample from the sample adding hole, the ultraviolet glue sample adding gun is used for injecting ultraviolet glue into the sample adding hole so as to seal the nucleic acid sample in the nucleic acid detection module.

Optionally, in the nucleic acid extraction detector, the loading gun and the ultraviolet glue loading gun are arranged at intervals along the extending direction of the first track portion, the liquid-moving portion moves along the first track portion to switch the loading gun to the filling position when the nucleic acid sample is injected into the loading hole, and moves along the first track portion to switch the ultraviolet glue loading gun to the filling position when the ultraviolet glue is injected into the loading hole.

Optionally, in the above nucleic acid extraction detector, the sample injection gun includes an injection device and a pushing block device, the injection device is used for extracting or injecting gas to the sample injection gun head, and the injection device is provided with a clamping protruding portion, the clamping protruding portion is used for being embedded into an inner cavity of the sample injection gun head, the pushing block device is movably connected to the sample injection gun, and the pushing block device moves towards the sample injection gun head, so that the sample injection gun heads which are clamped on the injection device are separated from each other through the pushing block device.

Optionally, in the above nucleic acid extraction detector, the nucleic acid detection module further includes a chip, the sample adding holes are formed in the chip, and the sample adding holes are plural, and each sample adding hole is uniformly distributed on the same circumference with the center of the chip as a center of a circle.

Optionally, in the nucleic acid extraction detector, the chip is rotatably mounted on the nucleic acid detection module, and the chip rotates around its own center for switching the sample loading hole located at the loading position.

Optionally, in the nucleic acid extraction detector, the nucleic acid detection module further includes a first heating plate and a second heating plate for heating, and the first heating plate is provided with a mounting ring for placing the chip, and the second heating plate is used for covering the chip located in the mounting ring.

Optionally, in the above nucleic acid extraction detector, the second heating plate is rotatably connected to the nucleic acid detection module, and/or; the nucleic acid detection module is also provided with a heat radiation fan group for radiating heat.

Alternatively, in the above nucleic acid extraction detector, the nucleic acid extraction module performs nucleic acid extraction on the sample solution by a magnetic sheath method.

When the nucleic acid extraction detector provided by the utility model is used, firstly, the nucleic acid extraction module extracts a nucleic acid sample from a sample solution, the extracted nucleic acid sample is placed in a reagent bottle, and the reagent bottle containing the nucleic acid sample is placed at an access position. Then the driving module starts to drive the pipetting part in the pipetting module to transport the nucleic acid sample placed at the access position to the filling position, and the nucleic acid detection sample is injected into the nucleic acid detection module at the filling position through the sample injection hole of the nucleic acid detection module, and finally the nucleic acid detection module completes the detection of the nucleic acid sample.

Compared with the prior art, the nucleic acid extraction detector provided by the utility model has the advantages that the nucleic acid extraction module is used for extracting the nucleic acid sample, and the driving module is used for driving the liquid-moving part in the liquid-moving module to transfer the nucleic acid sample to the nucleic acid detection module for detection, so that the automatic operation of nucleic acid transfer in the nucleic acid detection process is realized, the efficiency of nucleic acid work is improved, the operation and the interference of operators on the nucleic acid sample in the nucleic acid sample transfer process are reduced, the problems of diffusion pollution and disordered placement of the nucleic acid sample are reduced, the reliability of the operation of the nucleic acid detection is improved, and the accuracy of the detection result is ensured.

Drawings

In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram showing the structure of a nucleic acid extraction detector according to an embodiment of the present utility model;

FIG. 2 is a schematic diagram of a pipetting module according to an embodiment of the utility model;

FIG. 3 is a schematic view of a sample gun according to an embodiment of the present utility model;

FIG. 4 is a schematic diagram showing the structure of a nucleic acid detecting module according to an embodiment of the present utility model.

Wherein 100 is a nucleic acid extraction module, 110 is a reagent bottle accommodating box, and 120 is a slide rail device;

200 is a nucleic acid detection module, 210 is a chip, 211 is a sample adding hole, 220 is a first heating disc, 221 is a second heating disc, 230 is an ultraviolet spot lamp, 240 is a cooling fan set, 250 is a chip tray, 260 is a rotating motor, 270 is an optical detection module, 280 is a flip motor, and 290 is an air duct;

300 is a pipetting module, 310 is a pipetting part, 311 is a sample gun, 3111 is an injection device, 3111a is a clamping boss, 3112 is a pushing block device, 312 is an ultraviolet glue sample gun, 313 is a sample gun head, 320 is a first track part, 330 is a second track part, 340 is a second track part, and 350 is a sample gun head box;

400 is a drive module.

Detailed Description

The utility model aims at providing a nucleic acid extraction detector to improve the working efficiency and reliability of nucleic acid extraction detection.

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1, an embodiment of the present utility model discloses a nucleic acid extraction detector, which includes a nucleic acid extraction module 100, a nucleic acid detection module 200, a pipetting module 300, and a driving module 400. The nucleic acid extraction module 100 extracts a nucleic acid sample from a sample solution, places the extracted nucleic acid sample into a clean reagent bottle, places a reagent bottle containing the nucleic acid sample in the reagent bottle accommodating box 110 with an opening upward vertically, and places each reagent bottle in order, and places the reagent bottle accommodating box at a designated access position. When the pipetting section 310 moves to the access position, a nucleic acid sample placed at the access position is extracted. After the extraction, the liquid-transferring section 310 conveys the nucleic acid sample to the filling position, and after the filling position is reached, the nucleic acid sample is injected into the nucleic acid detecting module 200 through the sample-injecting hole 211, and finally the nucleic acid detecting module 200 detects the nucleic acid sample. The nucleic acid extraction detector integrates the extraction, transfer and detection of the nucleic acid sample, realizes high-efficiency automatic processing in the whole process, and remarkably improves the detection efficiency of the sample nucleic acid and the reliability of the nucleic acid detection.

As shown in fig. 1, the pipetting module 300 includes a first rail portion 320, a second rail portion 330 and a third rail portion 340, wherein the first rail portion 320 extends in a direction from the access position to the nucleic acid detecting module 200 to convey the nucleic acid sample located at the access position in the direction of the nucleic acid detecting module 200 to form a first movement direction, while the second rail portion 330 is movable in the extending direction of the first rail portion 320. And the second rail part 330 extends in the height direction of the nucleic acid detecting module 200, so that the position of the pipetting part 310 with respect to the height direction of the nucleic acid detecting module 200 can be adjusted to form a second movement direction, and the third rail part 340 can be moved in the extending direction of the second rail part 330. The third track portion 340 extends along the second track portion 330 to the filling position, and the pipetting portion 310 moves along the extending direction of the third track portion 340, so as to adjust the distance between the pipetting portion 310 and the filling position to form a third movement direction. The pipetting module 300 has a pipetting unit 310 movable in a first direction, a second direction and a third direction, wherein any two directions are perpendicular to each other, and the driving module 400 provides power for the movement of the pipetting unit 310, thereby realizing the three-dimensional movement of the pipetting unit 310 in space. In a specific embodiment, the second track portion 330 and the first track portion 320, the third track portion 340 and the second track portion 330, and the pipetting portion 310 and the third track portion 340 are all connected to each other by ball screw mechanisms to achieve the mutual movement between the tracks, and the driving module 400 is a stepper motor installed on each ball screw mechanism to drive the operation of the ball screw mechanism.

As shown in fig. 2, the pipette part 310 includes a loading gun 311 and an ultraviolet glue loading gun 312, the loading gun 311 is provided with a loading gun head 313, the nucleic acid sample in the reagent bottle positioned at the access position is extracted through the loading gun head 313, and the nucleic acid sample is contained in the loading gun head 313 at this time, and when the pipette part 310 conveys the loading gun 311 and the loading gun head 313 to the loading position, the nucleic acid sample in the loading gun head 313 is injected into the loading hole 211 through the loading gun 311 after the loading gun head 313 is matched with the loading hole 211 of the nucleic acid detection module 200. And the loading gun 311 and the loading gun head 313 are detachably connected, so that after each injection to the loading hole 211 is completed, the used loading gun head 313 is replaced by a new loading gun head 313, thereby avoiding pollution of the nucleic acid sample and ensuring accuracy of detection results. The ultraviolet glue is stored in the ultraviolet glue sample adding gun 312, after the sample adding gun 311 injects the nucleic acid sample into the nucleic acid detecting module 200 from the sample adding hole 211, the ultraviolet glue sample adding gun 312 injects the ultraviolet glue into the sample adding hole 211 to seal the sample adding hole 211, and the nucleic acid detecting module 200 further prevents the pollution of the outside to the nucleic acid sample in the nucleic acid sample detecting engineering.

In a specific embodiment, the loading gun 311 and the ultraviolet glue loading gun 312 are arranged at intervals along the extending direction of the first track portion 320, so that when the nucleic acid sample needs to be injected into the loading hole 211, the pipetting portion 310 moves along the first track portion 320 to move the loading gun 311 to the loading position, and the nucleic acid sample is injected into the loading hole 21. When the ultraviolet glue needs to be injected into the sample injection hole 211, the pipetting part 310 moves along the first track part 320 again to adjust the ultraviolet glue sample injection gun 312 to the injection position, and the ultraviolet glue is injected into the sample injection hole 21, so that the pipetting part 310 moves along the first track part 320 to realize the switching of the sample injection gun 311 and the ultraviolet glue sample injection gun 312 at the injection position.

As shown in fig. 3, the loading gun 311 includes an injection device 3111 and a pusher device 3112, and the injection device 3111 is used to extract or inject gas into the loading gun head 313, thereby achieving extraction and injection of the nucleic acid sample. And the outside of injection device 3111 is provided with joint protruding part 3111a, and joint protruding part 3111a is arranged in the inner chamber of embedding application of sample gun head 313 to application of sample gun 311 moves and stretches into the inner chamber of application of sample gun head 313 with joint protruding part 3111a of injection device 3111 to application of sample gun head 313 in application of sample gun head box 350, has realized application of sample gun 311 and application of sample gun head 313 installation fixedly. The pushing block device 3112 is movably connected to the loading gun 311, and the pushing block device 3112 is mounted on the loading gun 311 and moves toward the loading gun head 313, so that when the loading gun head 313 on the loading gun 311 needs to be replaced, the pushing block device 3112 moves toward the loading gun head 313 and pushes the loading gun head 313 off the loading gun 311, so that the injection device 3111 and the loading gun head 313 are separated from each other.

As shown in fig. 4, the nucleic acid detecting module 200 further includes a chip 210, and sample adding holes 211 are formed in the chip 210, so that in order to further improve the working efficiency of the nucleic acid detecting module 200 for detecting the nucleic acid sample, the sample adding holes 211 are provided in plurality, and each sample adding hole 211 is uniformly distributed on the same circumference of the chip 210 with the center of the chip 210 as the center of the circle.

Further, the chip 210 is rotatably mounted on the nucleic acid detecting module 200 with its own center as a rotation center, so that each sample loading hole 211 on the chip 210 can be sequentially switched to a loading position to cooperate with the sample loading gun 311 and the ultraviolet glue loading gun 312, and the nucleic acid sample and the ultraviolet glue are injected into each sample loading hole 211. After the injection of the nucleic acid sample and the ultraviolet glue into each sample-adding hole 211 of the chip 210 is completed, the ultraviolet light spot lamp 230 is turned on, so that the ultraviolet glue in each sample-adding hole 211 is rapidly solidified, and the filling and sealing treatment of the nucleic acid sample in the nucleic acid detection module 200 is completed. In a specific embodiment, the chip 210 is connected to a rotating motor 260 for driving the chip 210 to rotate through a chip tray 250, so that the chip 210 rotates on the nucleic acid detecting module 200.

In a specific embodiment, the nucleic acid detecting module 200 further includes a first heating plate 220 and a second heating plate 221 for heating, where the first heating plate 220 is provided with a mounting ring for mounting the chip 210, and when the chip 210 is mounted, the center position of the chip is coincident with the center position of the mounting ring, so that the first heating plate 220 uniformly surrounds the outer side and the bottom of the chip 210, thereby ensuring that each nucleic acid sample on the chip 210 is uniformly heated, and the second heating plate 221 seals the chip 210 in the mounting ring, and uniformly heats each nucleic acid sample. After the nucleic acid samples are heated to a preset temperature, each nucleic acid sample is detected by the optical detection module 270 on the nucleic acid detection module 200.

In addition, the second heating plate 221 is rotatably connected to the nucleic acid detecting module 200, in a specific embodiment, the second heating plate 221 is installed on the nucleic acid detecting module 200 through a rotation shaft, and a flip motor 280 is further provided to drive the second heating plate 221 to rotate, so as to further improve the working efficiency of the nucleic acid extraction detector provided in this embodiment. And/or, the nucleic acid detecting module 200 is further provided with a cooling fan set 240 for cooling, after the nucleic acid detecting module 200 completes the detection work of the nucleic acid sample, the flip motor 280 drives the second heating plate 221 to rotate so that the second heating plate 221 is opened, then the cooling fan set 240 is started, the air flow of the cooling fan set 240 flows to the first heating plate 220 and the chip 210 through the air duct 290, so that the cooling of the first heating plate 220 and the chip 210 is quickened, the time of the next processing of the chip 210 by staff is shortened, and the efficiency of the detection work of the nucleic acid sample is improved.

In another specific embodiment, the nucleic acid extraction module 100 performs nucleic acid extraction on the sample solution by a magnetic sleeve method, that is, in the process of extracting nucleic acid, magnetic beads are used as carriers, and the principle that the magnetic beads adsorb nucleic acid under high salt and low PH value and separate the nucleic acid under low salt and high PH value is utilized to realize the transfer of the magnetic beads, so as to complete the extraction and purification of nucleic acid. And nucleic acid extraction module 100 still is provided with and holds box 110 complex for slide rail device 120, and reagent bottle holds box 110 and is connected with the motor, holds box 110 and removes along slide rail device 120's extending direction through the motor drive reagent bottle for reagent bottle holds box 110 can accurately and arrive the access position fast, provides the preparation to the extraction and the transfer of pipetting module 300 to the nucleic acid.

It should be noted that, in the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described as different from other embodiments, and identical and similar parts between the embodiments are all enough to be referred to each other.

As used in this application and in the claims, the terms "a," "an," "the," and/or "the" are not specific to the singular, but may include the plural, unless the context clearly dictates otherwise. In general, the terms "comprises" and "comprising" merely indicate that the steps and elements are explicitly identified, and they do not constitute an exclusive list, as other steps or elements may be included in a method or apparatus. The inclusion of an element defined by the phrase "comprising one … …" does not exclude the presence of additional identical elements in a process, method, article, or apparatus that comprises an element.

Wherein, in the description of the embodiments of the present application, "/" means or is meant unless otherwise indicated, for example, a/B may represent a or B; "and/or" herein is merely an association relationship describing an association object, and means that three relationships may exist, for example, a and/or B may mean: a exists alone, A and B exist together, and B exists alone. In addition, in the description of the embodiments of the present application, "plurality" means two or more than two.

The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature.

The principles and embodiments of the present utility model have been described herein with reference to specific examples, the description of which is intended only to facilitate an understanding of the core concepts of the utility model. It should be noted that it will be apparent to those skilled in the art that various modifications and adaptations of the utility model can be made without departing from the principles of the utility model and these modifications and adaptations are intended to be within the scope of the utility model as defined in the following claims.

Claims

1. A nucleic acid extraction detector comprising:

a nucleic acid extraction module (100) for extracting a nucleic acid sample from the sample solution;

a nucleic acid detection module (200) for detecting a nucleic acid sample;

a pipetting module (300) comprising a pipetting section (310), the pipetting section (310) being configured to transfer a nucleic acid sample extracted by the nucleic acid extraction module (100) from an access position to a priming position and to inject the nucleic acid sample into the nucleic acid detection module (200) through a sample injection hole (211);

and a driving module (400) for driving the movement of the pipetting part (310).

2. The nucleic acid extraction detector of claim 1, wherein the pipetting module (300) further comprises a first rail portion (320), a second rail portion (330), and a third rail portion (340);

the first rail portion (320) extends in a direction from the access position to the nucleic acid detecting module (200) to form a first direction of movement, and the second rail portion (330) is movable in the direction of extension of the first rail portion (320);

the second rail portion (330) extends in a height direction of the nucleic acid detecting module (200) to form a second movement direction, and the third rail portion (340) is movable in an extending direction of the second rail portion (330);

the third track part (340) extends along the direction from the second track part (330) to the filling position to form a third movement direction, the liquid transferring part (310) moves along the extending direction of the third track part (340), and any two of the first movement direction, the second movement direction and the third movement direction are mutually perpendicular.

3. The nucleic acid extraction detector according to claim 2, characterized in that the pipetting part (310) comprises a loading gun (311) and an ultraviolet glue loading gun (312), the loading gun (311) extracts and injects a nucleic acid sample through a loading gun head (313), and the loading gun (311) is detachably connected with the loading gun head (313);

after the sample adding gun (311) is injected into a nucleic acid sample from the sample adding hole (211), the ultraviolet glue sample adding gun (312) is used for injecting ultraviolet glue into the sample adding hole (211) so as to seal the nucleic acid sample in the nucleic acid detection module (200).

4. The nucleic acid extraction detector according to claim 3, wherein the loading gun (311) and the ultraviolet gel loading gun (312) are arranged at intervals along the extending direction of the first rail portion (320), the pipetting part (310) is moved along the first rail portion (320) to switch the loading gun (311) to the loading position when the nucleic acid sample is injected into the loading hole (211), and the pipetting part (310) is moved along the first rail portion (320) to switch the ultraviolet gel loading gun (312) to the loading position when the ultraviolet gel is injected into the loading hole (211).

5. The nucleic acid extraction detector according to claim 3, wherein the loading gun (311) comprises an injection device (3111) and a pushing block device (3112), the injection device (3111) is used for extracting or injecting gas to the loading gun head (313), the injection device (3111) is provided with a clamping boss (3111 a), the clamping boss (3111 a) is used for being embedded into an inner cavity of the loading gun head (313), the pushing block device (3112) is movably connected to the loading gun (311), and the pushing block device (3112) moves towards the loading gun head (313) so as to separate the loading gun heads (313) clamped on the injection device (3111) from each other through the pushing block device (3112).

6. The nucleic acid extraction detector according to claim 1, wherein the nucleic acid detection module (200) further comprises a chip (210), the sample addition holes (211) are formed in the chip (210), and the plurality of sample addition holes (211) are provided, and each sample addition hole (211) is uniformly distributed on the same circumference with the center of the chip (210) as the center.

7. The nucleic acid extraction detector according to claim 6, characterized in that the chip (210) is rotatably mounted on the nucleic acid detection module (200), and the chip (210) rotates with its own center for switching the loading well (211) at the loading position.

8. The nucleic acid extraction detector according to claim 6, characterized in that the nucleic acid detection module (200) further comprises a first heating plate (220) and a second heating plate (221) for heating, and the first heating plate (220) is provided with a mounting ring for mounting the chip (210), and the second heating plate (221) is used for covering the chip (210) located in the mounting ring.

9. The nucleic acid extraction detector according to claim 8, characterized in that the second heating plate (221) is rotatably connected to the nucleic acid detection module (200), and/or; the nucleic acid detecting module (200) is further provided with a heat radiation fan group (240) for radiating heat.

10. The nucleic acid extraction detector according to any one of claims 1 to 9, characterized in that the nucleic acid extraction module (100) performs nucleic acid extraction on the sample solution by a magnetic sheath method.