CN220525728U - Electrophoresis rubberizing device - Google Patents
Electrophoresis rubberizing device Download PDFInfo
- Publication number
- CN220525728U CN220525728U CN202321926060.7U CN202321926060U CN220525728U CN 220525728 U CN220525728 U CN 220525728U CN 202321926060 U CN202321926060 U CN 202321926060U CN 220525728 U CN220525728 U CN 220525728U
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- China
- Prior art keywords
- glass plate
- comb
- short glass
- long glass
- electrophoresis
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 35
- 239000011521 glass Substances 0.000 claims abstract description 92
- 239000003292 glue Substances 0.000 claims abstract description 48
- 238000003860 storage Methods 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 230000005684 electric field Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001520 comb Anatomy 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to the technical field of electrophoresis experiments, in particular to an electrophoresis gel making device; the device comprises a fixing frame, a short glass plate, a long glass plate and a comb, wherein a storage groove is formed in the fixing frame, the storage groove is of a structure with an opening at the upper end, the short glass plate and the long glass plate are placed in the storage groove, a glue making gap is formed between the short glass plate and the long glass plate, the comb is arranged between the short glass plate and the long glass plate, and the comb extends into the glue making gap; the comb comprises a comb base body and comb teeth, wherein a plurality of comb teeth are arranged at the lower end of the comb base body, and the tooth width range of the comb teeth is set to be 0.1mm-0.3mm; the comb teeth are thinner, a thinner sample adding hole can be formed, the thinner sample adding hole can enable internal samples to be gathered, the sample gathering degree in the sample adding hole is improved, the interval of the final protein strips of electrophoresis is proper, the development presenting effect is improved, and the experimental result is more reliable and attractive.
Description
Technical Field
The utility model relates to the technical field of electrophoresis experiments, in particular to an electrophoresis gel making device.
Background
Electrophoresis is a general term of electrophoresis phenomenon, which refers to the phenomenon that charged particles move towards an electrode opposite to the charged particles under the action of an electric field, and a technology of separating the charged particles by utilizing different moving speeds of the charged particles in the electric field is called electrophoresis technology; electrophoresis is also used in western blotting, and in western blotting, which is now widely used in various aspects of expression studies of genes at protein level, detection of antibody activity, early diagnosis of diseases, etc., it is necessary to transfer proteins in cells or tissues separated by electrophoresis from a gel to a solid support and then detect a specific antigen with a specific antibody.
Before electrophoresis, the gel required by electrophoresis is required to be manufactured, most of the gel used by electrophoresis is configured according to actual needs by a laboratory, the conventional manufacturing device of the gel required by electrophoresis comprises a short glass plate, a long glass plate and a comb, the short glass plate and the long glass plate are connected through a bump and a groove in a clamping manner, a gap is formed between the short glass plate and the long glass plate, glue can be filled in the gap, and the comb stretches into the glue in the gap to form a sample adding hole, so that the gel required by electrophoresis can be obtained. However, the combs used in the laboratory are relatively wide in teeth, and the sample concentration in the sample application holes is insufficient, resulting in a thin final developed band. In addition, in the electrophoresis process, the boundary between the strips is not clear, and in severe cases, the strips are overlapped or connected together to form a black line, so that the development effect is poor, and the experimental result may be affected.
Therefore, compared with the conventional common gel preparation device, the improved electrophoresis gel preparation device has the advantages that the sample aggregation degree in the sample adding hole is improved, the interval between the final protein strips of electrophoresis is proper, the development presentation effect is improved, and the experimental result is more reliable and attractive.
Disclosure of Invention
The utility model solves the technical problems in the prior art, and provides an electrophoresis glue making device.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:
the electrophoresis glue making device comprises a fixing frame, a short glass plate, a long glass plate and a comb, wherein an object placing groove is formed in the fixing frame, the object placing groove is of a structure with an opening at the upper end, the short glass plate and the long glass plate are placed in the object placing groove, a glue making gap is formed between the short glass plate and the long glass plate, the comb is arranged between the short glass plate and the long glass plate, and the comb extends into the glue making gap to be arranged inside;
the comb comprises a comb base body and comb teeth, wherein a plurality of comb teeth are arranged at the lower end of the comb base body, and the tooth width range of the comb teeth is set to be 0.1mm-0.3mm.
Further, 10 comb teeth are arranged.
Further, the thickness of the comb was set to 1.0mm.
Further, the storage groove comprises a first through groove and a second through groove, the first through groove is communicated with the second through groove, the short glass plate is placed inside the first through groove, and the long glass plate is placed inside the second through groove.
Further, the long glass plate is provided with connecting strips near the left end, the right end and the lower end of the side wall of one side of the short glass plate, and the connecting strips are in contact with the short glass plate.
Further, the long glass plate is integrally connected with the connecting strip near the side wall of one side of the short glass plate, or the connecting strip is adhered to the side wall of one side of the long glass plate near the short glass plate.
Further, clamping pieces are respectively arranged at two ends of one side wall of the fixing frame, the clamping pieces are rotationally connected with the side wall of the fixing frame, and protruding blocks are arranged on the side wall of the clamping pieces.
Furthermore, the two ends of the lower part of the fixing frame are provided with supporting feet.
Compared with the prior art, the utility model has the beneficial effects that:
(1) According to the utility model, the short glass plate and the long glass plate are arranged in the storage groove of the fixing frame, a glue making gap is formed between the short glass plate and the long glass plate, a comb is inserted into the glue making gap, the tooth width range of the comb teeth is set to be 0.1mm-0.3mm, the comb teeth are thinner, thinner sample adding holes can be formed, the thinner sample adding holes can enable internal samples to be gathered, the sample gathering degree in the sample adding holes is improved, the interval of final protein strips of electrophoresis is proper, the development showing effect is improved, and the experimental result is more reliable and attractive.
Drawings
Fig. 1 is a schematic diagram of the overall structure of the present utility model.
Fig. 2 is a schematic view of the structure of the comb of the present utility model.
Reference numerals illustrate:
1. a fixing frame; 11. a storage groove; 12. a clamping piece; 13. supporting feet; 2. short glass plates; 3. a long glass plate; 4. a comb; 41. a comb base; 42. comb teeth.
Detailed Description
The technical solutions of the present utility model will be clearly described below with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present utility model, and all other embodiments obtained by a person skilled in the art without making any inventive effort are within the scope of protection of the present utility model. It should be noted that, the positional or positional relationship indicated by the terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
As shown in fig. 1, the utility model provides an electrophoresis glue making device, which comprises a fixing frame 1, a short glass plate 2, a long glass plate 3 and a comb 4, wherein a storage groove 11 is arranged in the fixing frame 1, the storage groove 11 is a through groove, the storage groove 11 is vertically arranged, the storage groove 11 comprises a first through groove and a second through groove, the first through groove is positioned at the front side of the second through groove, the first through groove is communicated with the second through groove, the front side is set to be close to one side of a user, the short glass plate 2 is placed in the first through groove, the long glass plate 3 is placed in the second through groove, a glue making gap is arranged between the short glass plate 2 and the long glass plate 3 after the short glass plate 2 and the long glass plate 3 are respectively placed in the first through groove and the second through groove, and the comb 4 is arranged at the upper end of the glue making gap.
The connecting strip of U type is equipped with on one side of long glass board 3, and the connecting strip sets up the left and right both ends and the lower extreme at long glass board 3 lateral wall, and after long glass board 3 stretched into the second through groove inside, the connecting strip is close to long glass board 3 one side lateral wall contact with short glass board 2, forms between short glass board 2, connecting strip and the long glass board 3 and makes gluey clearance, and the connecting strip sets up or pastes the setting with long glass board 3 body coupling.
The front side of the fixing frame 1 is provided with two clamping pieces 12, the clamping pieces 12 are rotationally connected to the side wall of the front side of the fixing frame 1, the two clamping pieces 12 are respectively arranged at the left end and the right end of the side wall of the front side of the fixing frame 1, the side wall of the clamping piece 12 is provided with a lug, and when the clamping pieces 12 rotate to be parallel to the storage groove 11, the lug arranged by the clamping pieces 12 is extruded with the side wall of the end part of the storage groove 11 corresponding to the fixing frame 1 and is used for clamping the short glass plate 2 and the long glass plate 3; both ends of the lower part of the fixing frame 1 are provided with supporting feet 13.
As shown in fig. 2, the comb 4 includes a comb base 41 and a plurality of comb teeth 42, the lower end of the comb base 41 is integrally connected to form a plurality of comb teeth 42, the plurality of comb teeth 42 are arranged along the length direction of the comb base 41 at intervals, the comb teeth 42 are preferably arranged to be 10, the thickness of the comb 4 is set to be 1.0mm, the tooth width range of the comb teeth 42 is set to be 0.1mm-0.3mm, and is preferably set to be 0.22mm, the tooth width of the comb teeth 42 is smaller, thinner sample adding holes can be formed, the thinner sample adding holes can enable internal samples to be gathered, the interval is proper, the developing effect is better, the sample adding strips are clear due to sample gathering, and the difference between the samples is easier to see, so that the experimental success rate is improved.
The utility model provides a working principle of an electrophoresis gel making device, which comprises the following steps: the fixing frame 1 is placed on the upper portion of a glass frame (shown in the figure), the fixing frame 1 is horizontally placed, two clamping pieces 12 are placed in the state of being perpendicular to the direction of the object placing groove 11, after the short glass plate 2 and the long glass plate 3 are cleaned and dried, the short glass plate 2 is placed in the first through groove, the long glass plate 3 is placed in the second through groove, the lower end of the short glass plate 2 is aligned with the lower end of the long glass plate 3, at the moment, the upper end of the long glass plate 3 extends out of the short glass plate 2, the clamping pieces 12 are enabled to rotate towards the direction close to the direction parallel to the object placing groove 11 until the clamping pieces 12 are in the state parallel to the object placing groove 11, at the moment, the protruding blocks arranged by the clamping pieces 12 squeeze the object placing groove 11, the short glass plate 2 and the long glass plate 3 are clamped, the connecting strips connected with the short glass plate 2 and the long glass plate 3 are tightly attached, and the situation of glue leakage of subsequently added separating glue or concentrated glue is effectively prevented.
After the short glass plate 2 and the long glass plate 3 are fixed in the fixed frame 1, 10 percent of separating glue is configured, then TEMED (tetramethyl ethylenediamine) is added into the separating glue and immediately shaken up to be filled with glue, when the glue is filled, a 10ml glue filling gun can be used for sucking 5ml of separating glue and discharging along a glue making gap, the glue carrying surface rises to the middle height of the fixed frame 1 to stop filling the separating glue, then a layer of water is added into the separating glue for liquid sealing, and the solidification of the separating glue can be accelerated after the liquid sealing; in the process of filling the separating glue, the speed can be increased at the beginning, the speed is reduced when the glue surface is as high as required, the separating glue must flow down along the glass plate during operation, the bubbles in the separating glue can be prevented, the speed is very low when the water is added for liquid sealing, and the separating glue is prevented from being punched and deformed by water; when a fold line is formed between the water and the separating glue, the separating glue is solidified, and then the separating glue stands still for 3 minutes, the water removing layer is poured, and the water absorbing paper is used for absorbing the water.
Then preparing 4% of concentrated glue, adding TEMED (tetramethyl ethylenediamine) into the concentrated glue, shaking up immediately, filling the concentrated glue into the residual space of the glue making gap, inserting the comb teeth 42 of the comb 4 into the concentrated glue, keeping the comb 4 horizontal when the comb 4 is inserted, and because the volume is reduced when the glue is solidified, the glue needs to be frequently supplemented on two sides in the process of solidifying the concentrated glue, after the concentrated glue is solidified, holding the two ends of the comb 4 by hands, uniformly exerting force, pulling up the comb 4, taking out the short glass plate 2 and the long glass plate 3 together from the inside of the fixing frame 1, and then integrally placing the short glass plate 2 and the long glass plate 3 into an electrophoresis tank, thus completing electrophoresis glue making.
Finally, it should be noted that the above description is only for illustrating the technical solution of the present utility model, and not for limiting the scope of the present utility model, and that the simple modification and equivalent substitution of the technical solution of the present utility model can be made by those skilled in the art without departing from the spirit and scope of the technical solution of the present utility model.
Claims (8)
1. The electrophoresis glue making device is characterized by comprising a fixing frame, a short glass plate, a long glass plate and a comb, wherein an object placing groove is formed in the fixing frame, the object placing groove is of a structure with an opening at the upper end, the short glass plate and the long glass plate are placed in the object placing groove, a glue making gap is formed between the short glass plate and the long glass plate, the comb is arranged between the short glass plate and the long glass plate, and the comb extends into the glue making gap to be arranged inside;
the comb comprises a comb base body and comb teeth, wherein a plurality of comb teeth are arranged at the lower end of the comb base body, and the tooth width range of the comb teeth is set to be 0.1mm-0.3mm.
2. An electrophoresis gel device according to claim 1 wherein the number of teeth is 10.
3. An electrophoresis gel producing apparatus according to claim 2 wherein the thickness of the comb is set to 1.0mm.
4. An electrophoresis gel device according to claim 1 wherein the storage slot comprises a first through slot and a second through slot, the first through slot and the second through slot are in communication, the short glass plate is placed inside the first through slot, and the long glass plate is placed inside the second through slot.
5. An electrophoresis gel producing device according to claim 4 wherein the long glass plate is provided with connecting strips at the left end, right end and lower end near the side wall of one side of the short glass plate, and the connecting strips are in contact with the short glass plate.
6. An electrophoresis gel producing device according to claim 5 wherein the long glass plate is integrally connected with the connecting strip near the side wall of the short glass plate, or the long glass plate is adhered with the connecting strip near the side wall of the short glass plate.
7. The electrophoresis gel making device according to claim 1, wherein clamping pieces are respectively arranged at two ends of a side wall of one side of the fixing frame, the clamping pieces are rotatably connected with the side wall of the fixing frame, and protruding blocks are arranged on the side wall of the clamping pieces.
8. An electrophoresis gel making device according to claim 7 wherein the two ends of the lower part of the fixing frame are provided with supporting feet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321926060.7U CN220525728U (en) | 2023-07-21 | 2023-07-21 | Electrophoresis rubberizing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321926060.7U CN220525728U (en) | 2023-07-21 | 2023-07-21 | Electrophoresis rubberizing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220525728U true CN220525728U (en) | 2024-02-23 |
Family
ID=89938422
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321926060.7U Active CN220525728U (en) | 2023-07-21 | 2023-07-21 | Electrophoresis rubberizing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220525728U (en) |
-
2023
- 2023-07-21 CN CN202321926060.7U patent/CN220525728U/en active Active
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