CN212932485U - A die carrier supports box for protein and nucleic acid polyacrylamide gel electrophoresis - Google Patents
A die carrier supports box for protein and nucleic acid polyacrylamide gel electrophoresis Download PDFInfo
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- CN212932485U CN212932485U CN202022029239.5U CN202022029239U CN212932485U CN 212932485 U CN212932485 U CN 212932485U CN 202022029239 U CN202022029239 U CN 202022029239U CN 212932485 U CN212932485 U CN 212932485U
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Abstract
The utility model discloses a die carrier supports box for protein and nucleic acid polyacrylamide gel electrophoresis, including notch plate, rectangular plate, porous one-tenth appearance ware and instruction comb hole of going up. The utility model discloses do not change the separation and glue the prescription, only the die carrier through the electrophoresis of the protein of contracting and nucleic acid polyacrylamide gel in the specific area supports box and inner space in the external structure, the sample becomes constrictive through the passageway in this area, thereby provide sample compression effect, play important effect for the simplification of system glue process, and the concentrated glue that does not influence protein or nucleic acid detection has been cancelled, electrophoresis experiment system glue process has not only been simplified, also make the success rate of gel configuration improve greatly simultaneously, solve extravagant problem, die carrier through the electrophoresis of the protein of contracting and nucleic acid polyacrylamide gel in the specific area supports box and inner space, make the sample become constrictive through the passageway in this area, thereby provide sample compression effect, play very important effect for the simplification of system glue process.
Description
Technical Field
The utility model relates to a biochemical detection technical field specifically is a die carrier supports box that is used for protein and nucleic acid polyacrylamide gel electrophoresis.
Background
Polyacrylamide gel is a material used in the biomedical field for biochemical tests. It can isolate protein or nucleic acid molecules, and is typically used to analyze sample components.
The gel is mainly composed of acrylamide solution, buffer solution, deionized water, coagulant and catalyst to form glue solution with different concentrations, and under the action of coagulant and catalyst, the solution forms a space net structure which plays a role of molecular sieve when separating protein or nucleic acid.
In the electrophoresis process, the charges carried by the protein or the nucleic acid can directionally migrate under the action of an electric field, and are finally separated by gel according to the molecular weight, the charges and the space structure of the protein or the nucleic acid, so that the purpose of analyzing the protein or the nucleic acid is achieved.
The polyacrylamide gel is divided into two parts of concentrated gel and separation gel on the component structure, the concentration of the concentrated gel is lower than that of the separation gel, in the pouring process, when the separation gel is in a liquid state, the concentrated gel solution is gently poured above the separation gel, two gel solutions with different concentrations are layered, the two solutions are not mixed as much as possible, otherwise the poured gel cannot be used, because the technique is difficult to ensure one-time success, needs a great deal of time and experience to be skilled, greatly restricts the experimental process, therefore, the invention adds a compression device through the change of the external structure, and through the die carrier supporting box and the internal space of the polyacrylamide gel electrophoresis of the condensed protein and nucleic acid in a specific area, the passage of the sample through the area becomes narrow, so that the sample compression effect is provided, and the important function is played for simplifying the glue preparation process; meanwhile, the experiment personnel are provided with one solution less, and for experiments, the solution is provided less, so that the experiment time is saved, the process of easily preparing wrong glue solution is omitted, and the success of the experiments is greatly improved.
In order to facilitate the experiment without affecting the experimental results and provide the concentration effect for the protein or nucleic acid sample without the concentrated gel, the following technical problems need to be solved:
1. the experimental result is not influenced. The stability of the formula provides an important guarantee for the successful development of experiments, once, the components and the proportion of the separating gel formula cannot be changed, and only a device capable of achieving the concentrating effect can be added from the outside.
2. Is suitable for common electrophoresis tanks in the market. The protein and nucleic acid polyacrylamide gel electrophoresis mould frame support box can not be greatly improved in the external structure, which can increase the error rate of the device in the electrophoresis tank, so that the article needs to be made on the internal structure.
3. The glue solution can be conveniently poured. Although the components are structurally divided into concentrated glue and separation glue, the separation glue is only used for testing a sample, the concentrated glue is only used for compressing the sample, and the two glue solutions can not be mixed when being poured at the same time, which is a very difficult and troublesome thing.
To this end, we propose a scaffold support cassette for protein and nucleic acid polyacrylamide gel electrophoresis.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a die carrier supports box for protein and nucleic acid polyacrylamide gel electrophoresis to solve the problem that proposes in the background art.
In order to achieve the above object, the utility model provides a following technical scheme: the die carrier supporting box comprises a concave plate, a rectangular plate, a porous sample loading former and an indication comb hole, wherein the concave plate consists of a plate opening, a compression plate, a sample loading notch, positioning holes, sealing grooves and a second forming plate, the two sides of the second forming plate are provided with protrusions, the top of the inner concave surface of the second forming plate is provided with the concave sample loading notch, the inner concave surface of the second forming plate is provided with the convex compression plate below the sample loading notch, the two sides of the second forming plate are provided with the positioning holes which are symmetrically distributed, the tops of the two sides of the second forming plate are provided with plate openings, and the two sides of the second forming plate are provided with the convex sealing grooves;
the rectangular plate consists of a compression groove, a second glue storage groove, a positioning column and a third molding plate, the positioning column is fixedly connected to the surface of the third molding plate and corresponds to the positioning hole, the compression groove is formed in the surface of the third molding plate and corresponds to the compression plate, and the second glue storage groove which is concave and used for storing gel is formed in the surface of the third molding plate and above the compression groove;
the multi-hole sample loading former consists of a first forming plate, a comb plate, a pulling groove and a first glue storage groove, wherein the comb plate matched with the sample loading notch is correspondingly and fixedly connected to the bottom of one side of the first forming plate, and the surface of the first forming plate is provided with the first glue storage groove for storing gel;
the second molding plate and the third molding plate are mutually clamped through a positioning hole formed in the surface of the second molding plate and a positioning column on the surface of the third molding plate, the positioning hole and the positioning column which are mutually clamped can fix the second molding plate and the third molding plate, the first molding plate is inserted into the top opening of the second molding plate and the top opening of the third molding plate, and the comb plate at the bottom of the first molding plate is clamped into the sample feeding notch on the surface of the second molding plate.
Furthermore, the front surface and the back surface of the first molding plate and the upper part of the first glue storage groove are provided with pulling grooves.
Further, the comb plate and the sample loading notch are both arranged to be comb-shaped structures.
Furthermore, the back of the concave plate is provided with an indication comb hole corresponding to the position of the sample loading notch.
Compared with the prior art, the utility model discloses following beneficial effect has:
1. the experimental result is not influenced, the formula of the separation gel is not changed, and the passage of the sample through the specific region is narrowed only on the external structure by the die carrier supporting box and the internal space of the condensation protein and nucleic acid polyacrylamide gel electrophoresis in the specific region, so that the compression effect of the sample is provided, and the important effect is played for the simplification of the gel preparation process;
2. the glue solution can be conveniently poured. The concentrated gel which does not affect the detection of protein or nucleic acid is cancelled, thereby not only simplifying the gel preparation process of the electrophoresis experiment, but also greatly improving the success rate of gel preparation.
3. The problem of waste is solved. Through the die carrier support box and the inner space of the polyacrylamide gel electrophoresis of the protein and the nucleic acid in the specific area, the passage of the sample through the area becomes narrow, thereby providing the compression effect of the sample, playing an important role in simplifying the gel making process, and the change can facilitate the gel configuration and simplify the electrophoresis experiment process.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic perspective view of the concave plate of the present invention;
FIG. 3 is a schematic view of the three-dimensional structure of the rectangular plate of the present invention;
fig. 4 is the three-dimensional structure schematic diagram of the porous large-capacity sample-loading former of the utility model.
In the figure: 1 concave plate, 2 rectangular plates, 3 porous large-capacity sample loading formers, 4 indication comb holes, 5 plate openings, 6 first forming plates, 7 comb plates, 8 pulling grooves, 9 first glue storage grooves, 10 compression grooves, 11 second glue storage grooves, 12 positioning columns, 13 compression plates, 14 sample loading groove openings, 15 positioning holes, 16 sealing grooves, 17 second forming plates and 18 third forming plates.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a die carrier supporting box for protein and nucleic acid polyacrylamide gel electrophoresis comprises a concave plate 1, a rectangular plate 2, a porous sample loading former 3 and an indication comb hole 4, wherein the concave plate 1 consists of a plate opening 5, a compression plate 13, a sample loading notch 14, a positioning hole 15, a sealing groove 16 and second molding plates 17 with two sides provided with protrusions, the second molding plates 17 are arranged in a concave manner, the top of the inner concave surface of each second molding plate 17 is provided with the concave sample loading notch 14, the lower part of the inner concave surface of each second molding plate 17, which is positioned below the sample loading notch 14, is provided with the convex compression plate 13, the two sides of each second molding plate 17 are provided with the positioning holes 15 which are symmetrically distributed, the top of the two sides of each second molding plate 17 is provided with the plate opening 5, the two sides of each second molding plate 17 are provided with the convex sealing groove 16, and the compression plates 13 can be used without a gel making method, the sample compression effect is provided for the gel, and the method plays a very important role in simplifying the gel preparation process;
the rectangular plate 2 consists of a compression groove 10, a second glue storage groove 11, a positioning column 12 and a third molding plate 18, the positioning column 12 is fixedly connected to the surface of the third molding plate 18 and corresponds to the position of the positioning hole 15, the compression groove 10 is formed in the surface of the third molding plate 18 and corresponds to the position of the compression plate 13, the second glue storage groove 11 which is concave and used for storing gel is formed in the surface of the third molding plate 18 and is positioned above the compression groove 10, and the compression groove 10 enables a passage of a sample passing through the region to be narrow through a die carrier support box for condensing protein and nucleic acid polyacrylamide gel electrophoresis and an internal space in a specific region, so that a sample compression effect is provided, and a very important role is played in simplifying a glue making process; the second glue storage tank 11 can store glue solution, and plays an important role in the integrity of the gel;
the multi-hole sample loading former 3 comprises a first forming plate 6, a comb plate 7, a pulling groove 8 and a first glue storage groove 9, the bottom of one side of the first forming plate 6 is correspondingly and fixedly connected with the comb plate 7 matched with a sample loading notch 14, the surface of the first forming plate 6 is provided with the first glue storage groove 9 for storing gel, and the comb plate 7 with a comb-shaped structure is an important structure for forming a comb hole space; meanwhile, the first glue storage tank 9 on the surface of the first forming plate 6 can store glue solution, and plays an important role in the integrity of the gel;
the second forming plate 17 and the third forming plate 18 are mutually clamped through a positioning hole 15 formed in the surface of the second forming plate 17 and a positioning column 12 formed in the surface of the third forming plate 18, the positioning hole 15 and the positioning column 12 which are mutually clamped can fix the second forming plate 17 and the third forming plate 18, the first forming plate 6 is inserted into top openings of the second forming plate 17 and the third forming plate 18, and the comb plate 7 at the bottom of the first forming plate 6 is clamped into an upper sample notch 14 formed in the surface of the second forming plate 17.
Further, the front and the back of the first molding plate 6 and the upper side of the first glue storage groove 9 are both provided with a pulling groove 8, the pulling groove 8 is arranged to increase the contact friction force, so that the experimenter can take out the porous sample-feeding former 3 conveniently.
Further, the comb plate 7 and the sample loading notch 14 are both arranged to be in a comb-tooth structure.
Further, the back of concave plate 1 and the position that corresponds to sample loading notch 14 are provided with and indicate comb hole 4, indicate comb hole 4 to be one row of structure of indicating the comb hole position, make things convenient for the experimenter to find transparent sample loading hole in transparent solution when the appearance of loading.
When the device is used, firstly, the second forming plate 17 and the third forming plate 18 are mutually clamped through the positioning hole 15 arranged on the surface of the second forming plate 17 and the positioning column 12 arranged on the surface of the third forming plate 18, the second forming plate 17 and the third forming plate 18 can be fixed through the positioning hole 15 and the positioning column 12 which are mutually clamped, then the second forming plate 17 and the third forming plate 18 are fixed through the sealing strip, then the configured glue solution is poured into a gap between the concave position of the second forming plate 17 and the third forming plate 18, then the first forming plate 6 and the comb plate 7 are inserted into the position of the sample loading notch 14 between the second forming plate 17 and the third forming plate 18, after the device is installed, the comb plate 7 at the bottom of the first forming plate 6 can be mutually overlapped with the position of the sample loading notch 14, and the convex compression groove 10 and the compression plate 13 have a certain compression effect in the process of inserting the comb plate 7, therefore, concentrated glue solution does not need to be prepared in the glue preparation process, only separated glue solution needs to be prepared, after the gel in the box is solidified, the gel is placed in an electrophoresis tank, electrophoresis buffer solution is poured in, the porous sample loading former 3 is pulled out, the comb plate 7 is separated from the inside of the sample loading notch 14 by the pulled porous sample loading former 3, and then a protein or nucleic acid sample is added, so that the experiment can be carried out.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. The utility model provides a die carrier supports box for protein and nucleic acid polyacrylamide gel electrophoresis, includes notch plate (1), rectangular plate (2), porous former (3) of going up and instructs broach (4), its characterized in that: the concave plate (1) consists of a plate opening (5), a compression plate (13), sample loading notches (14), positioning holes (15), sealing grooves (16) and second molding plates (17) with bulges at two sides, the top of the inner concave surface of each second molding plate (17) is provided with the concave sample loading notches (14), the concave surfaces of the second molding plates (17) are provided with the convex compression plates (13) below the sample loading notches (14), the two sides of each second molding plate (17) are provided with the positioning holes (15) which are symmetrically distributed, the top of the two sides of each second molding plate (17) is provided with the plate opening (5), and the two sides of each second molding plate (17) are provided with the convex sealing grooves (16);
the rectangular plate (2) is composed of a compression groove (10), a second glue storage groove (11), a positioning column (12) and a third molding plate (18), the positioning column (12) is fixedly connected to the surface of the third molding plate (18) and corresponds to the position of the positioning hole (15), the compression groove (10) is formed in the surface of the third molding plate (18) and corresponds to the position of the compression plate (13), and the concave second glue storage groove (11) for storing gel is formed in the surface of the third molding plate (18) and above the compression groove (10);
the multi-hole sample loading former (3) comprises a first forming plate (6), a comb plate (7), a pulling groove (8) and a first glue storage groove (9), the bottom of one side of the first forming plate (6) is correspondingly and fixedly connected with the comb plate (7) matched with a sample loading notch (14), and the surface of the first forming plate (6) is provided with the first glue storage groove (9) for storing gel;
the second forming plate (17) and the third forming plate (18) are mutually clamped through a positioning hole (15) formed in the surface of the second forming plate (17) and a positioning column (12) formed in the surface of the third forming plate (18), the positioning hole (15) and the positioning column (12) which are mutually clamped can fix the second forming plate (17) and the third forming plate (18), the first forming plate (6) is inserted into the top opening of the second forming plate (17) and the top opening of the third forming plate (18), and the comb plate (7) at the bottom of the first forming plate (6) is clamped into an upper sample notch (14) formed in the surface of the second forming plate (17).
2. The scaffold support cassette for protein and nucleic acid polyacrylamide gel electrophoresis of claim 1, wherein: and the front surface and the back surface of the first molding plate (6) and the upper part of the first glue storage groove (9) are provided with pulling grooves (8).
3. The scaffold support cassette for protein and nucleic acid polyacrylamide gel electrophoresis of claim 1, wherein: the comb plate (7) and the sample loading notch (14) are both arranged to be comb-shaped structures.
4. The scaffold support cassette for protein and nucleic acid polyacrylamide gel electrophoresis of claim 1, wherein: and an indication comb hole (4) is arranged on the back surface of the concave plate (1) and corresponds to the position of the sample loading notch (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022029239.5U CN212932485U (en) | 2020-09-16 | 2020-09-16 | A die carrier supports box for protein and nucleic acid polyacrylamide gel electrophoresis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022029239.5U CN212932485U (en) | 2020-09-16 | 2020-09-16 | A die carrier supports box for protein and nucleic acid polyacrylamide gel electrophoresis |
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| CN212932485U true CN212932485U (en) | 2021-04-09 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114235933A (en) * | 2021-12-22 | 2022-03-25 | 安徽昊拓生物科技有限公司 | Dye of colored polyacrylamide gel and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114235933A (en) * | 2021-12-22 | 2022-03-25 | 安徽昊拓生物科技有限公司 | Dye of colored polyacrylamide gel and preparation method thereof |
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