CN210906099U

CN210906099U - Gel preparation facilities of SDS-PAGE

Info

Publication number: CN210906099U
Application number: CN201921708032.1U
Authority: CN
Inventors: 王磊; 韩庆军; 栗宁宁; 张峰; 姚树建; 刘涛
Original assignee: CENTER FOR FOREST GENETIC RESOURCES OF SHANDONG PROVINCE
Current assignee: CENTER FOR FOREST GENETIC RESOURCES OF SHANDONG PROVINCE
Priority date: 2019-10-12
Filing date: 2019-10-12
Publication date: 2020-07-03
Anticipated expiration: 2029-10-12

Abstract

The utility model discloses a gel preparation facilities of SDS-PAGE, including back plate and front bezel, the back plate both sides set up the space bar respectively, set up the breach on the space bar, the front bezel comprises preceding upper plate and preceding hypoplastron. The utility model discloses saved the waiting time that the separation glue gel solidifies, the rational utilization electrophoresis apparatus equipment and the point appearance time, reduced the possibility of leaking glue moreover to a certain extent.

Description

Gel preparation facilities of SDS-PAGE

Technical Field

The utility model relates to a gel preparation facilities of SDS-PAGE.

Background

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an important means for separating proteins according to their molecular weights. SDS-PAGE generally uses a discontinuous buffer system, which allows higher resolution than a continuous buffer system. However, the separation gel and the concentrated gel in the discontinuous buffer system need to be arranged in sequence, and the separation gel and the concentrated gel take a long time to solidify after the arrangement. Because the gel can only realize better separation effect if the solidification is sufficient, so the solidification time is inevitable, other works are mainly arranged through the solidification time at present to improve the time utilization rate, the solidification can also be accelerated by raising the temperature, but the separation effect is not good due to the accelerated solidification.

In addition, the gel device is not tightly assembled, so that the gel leakage phenomenon can occur, and the time can be wasted due to the reconfiguration after the gel leakage. Glue leakage is a phenomenon that glue solution flows out through a gap before solidification under the action of gravity, because a common gap is not too large, the glue solution flows out slowly, and in addition, due to the existence of the solidification phenomenon, the glue solution generally only flows out of a small part, but the small part of the glue solution also inevitably leads to rework.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model provides a gel preparation device of SDS-PAGE has saved the waiting time that the gel of separating glue solidifies, has reasonably utilized the assembly of an electrophoresis device and the sample application time, and reduces the possibility of glue leakage to a certain extent.

The utility model relates to a gel preparation facilities of SDS-PAGE, the concrete scheme is as follows:

the utility model provides a gel preparation facilities of SDS-PAGE, includes back plate and front bezel, back plate both sides set up the spacer respectively, set up the breach on the spacer, the front bezel comprises preceding upper plate and preceding lower plate.

Further, the two spacing pieces are arranged in parallel.

Further, the gap divides the spacer into an upper spacer and a lower spacer.

Further, after the rear plate and the front plate are assembled, the gap corresponds to a gap between the front upper plate and the front lower plate.

Furthermore, the upper edge of the front upper plate is provided with a notch, and plate lugs are formed on two sides of the notch.

The sealing strip is formed by connecting a bottom sealing strip, a side sealing strip and a middle sealing strip into a whole.

Further, the sealing strip is used for plugging a gap between the rear plate and the front plate so as to ensure that glue liquid cannot leak out in the glue making process.

Further, the bottom seal is used for sealing a gap between the bottom of the front lower plate and the rear plate, the side seal is used for sealing a gap between the rear plate and two sides of the front plate, and the middle seal is used for sealing a gap between the bottom of the front upper plate and the rear plate.

Furthermore, the section of the middle seal strip is in an L shape or an ⊥ shape.

Furthermore, the sealing strip is provided with a first through hole at the junction of the side sealing strip and the middle sealing strip.

Further, the first through hole is opened in a space enclosed by the bottom seal, the side seals and the middle seal, and the assembled opening faces a gap between the front lower plate and the rear plate.

Further, the first through hole is arranged from inside to outside in an inclined manner.

Further, the first through hole wall is made of a hard material.

Furthermore, the sealing device also comprises a sealing block, and the sealing block is provided with a second through hole.

Further, the second through hole is formed in the upper center of the sealing block.

Further, the second through hole wall is made of a hard material.

Further, the adhesive tape is also included.

Further, the adhesive tape is used for sealing a gap between the front upper plate and the front lower plate.

The utility model discloses still relate to a SDS-PAGE's gel preparation facilities's application method, the step is as follows:

1) the back plate is placed horizontally, and the surface of the spacer is upward;

2) the sealing strip is placed on the rear plate, the bottom sealing strip is placed below the spacing piece, the side sealing strip is placed on the outer side of the spacing piece, and the middle sealing strip is placed in the gap of the spacing piece;

3) the front upper plate of the front plate is placed on the upper spacing piece, the front lower plate is placed on the lower spacing piece, and the rear plate and the front plate are clamped tightly;

4) separating glue is added into a gap between the front lower plate and the rear plate, concentrating glue is added into a gap between the front upper plate and the rear plate, and a comb is inserted;

5) and after the gel is formed, taking down the sealing strip, placing the sealing block into the gap, sealing the gap between the front upper plate and the front lower plate by using the adhesive tape, and injecting connecting glue between the concentrated glue and the separating glue.

Compared with the prior art, the beneficial effects of the utility model are that:

1. gel preparation facilities divide into preceding upper plate and preceding hypoplastron with the front bezel among the prior art, set up the breach on the spacer, realized concentrated glue and separation glue preparation in the time, saved the separation and glued the latency that the gel solidifies, rational utilization electrophoresis apparatus equipment and some appearance time, simultaneously, prevent to a certain extent to leak and glue.

2. Connecting gel is injected between the concentrated gel and the separating gel, and the components can be the concentrated gel or the separating gel, and other gels different from the concentrated gel and the separating gel can be selected. The connection glue has small occupation ratio and can be put into the electrophoresis tank immediately after being added for spotting electrophoresis, and because the assembly and spotting process of the electrophoresis tank needs a period of time, the connection glue can realize solidification.

3. The setting of breach on the one hand is favorable to preceding upper plate and the position of preceding hypoplastron to be confirmed, and on the other hand provides the accommodation space of adhesive tape, makes the adhesive tape become the integral structure and becomes possible.

4. The sealing strip encloses the space where the separating glue is located into a closed space, and even if a small gap is formed in the local part, liquid leakage cannot occur. Because the open space in top is different with inclosed space, the top is opened and is glued the liquid outflow under the action of gravity and be inevitable, and the airtight glue liquid in top still receives atmospheric pressure's counteraction when receiving gravity for even there is the risk of certain gap weeping in the bottom also relatively to reduce.

5. The design of first through-hole and second through-hole makes the injection of glue solution easier, can adopt the syringe needle to pierce when not having the through-hole, and this process probably can make sealing strip or sealing block warp and produce the gap, and the glue solution is injected and is accomplished the back and is sealed the through-hole with the glue solution and guarantee the leakproofness.

6. First through-hole opening is towards the separation glue space, sets up from inside to outside slant in addition, guarantees that the syringe needle reaches the lateral wall after inserting, makes the glue solution leave along the lateral wall, reduces the possibility that the bubble produced.

7. The second through hole is arranged at the upper part of the center of the sealing block, which is beneficial to adjusting the contact of the needle head and the bottom of the concentrated glue, and removing bubbles in the glue adding process, so that the connection glue is tightly connected with the concentrated glue and the separation glue.

8. The wall of each of the first through hole and the second through hole is made of a hard material, so that the rigidity of the through holes is ensured, and the needle cannot be inserted due to excessive deformation in the extrusion process.

9. The adhesive tape is an insulating material and is used for sealing a gap between the front upper plate and the front lower plate. Ensure the standard movement of the sample in the gel under the action of the positive and negative electrodes.

10. The section of the middle seal strip is L-shaped or ⊥ -shaped, the L-shaped can be deeply embedded into the bottom of the concentrated glue area, and the ⊥ -shaped can be deeply embedded into the top of the separation glue at the bottom of the concentrated glue area, so that the tightness of a gel space is further ensured.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic diagram of the rear plate structure of the present invention;

FIG. 3 is a schematic diagram of the front plate structure of the present invention;

FIG. 4 is a schematic view of the sealing strip structure of the present invention;

FIG. 5 is a schematic view of the structure of the position relationship of the seal strip of the present invention;

FIG. 6 is a schematic view of the sealing block structure of the present invention;

FIG. 7 is a schematic view of the comb structure of the present invention;

fig. 8 is a schematic view of the adhesive tape of the present invention.

The novel comb comprises a rear plate 1, a front plate 2, a sealing strip 3, a sealing block 4, a comb 5, an adhesive tape 6, a spacer 11, a notch 12, a notch 21, a front upper plate 22, a front lower plate 23, a notch 24, a plate lug 31, a bottom sealing strip 32, a side sealing strip 33, a middle sealing strip 34, a first through hole 41, a second through hole 111, an upper spacer and a lower spacer 112.

Detailed Description

Example one

As shown in fig. 1, 2 and 3, the gel preparation device for SDS-PAGE comprises a back plate 1 and a front plate 2, wherein spacers 11 are respectively arranged on two sides of the back plate 1, notches 12 are arranged on the spacers 11, and the front plate 2 is composed of a front upper plate 21 and a front lower plate 22.

Further, two of the spacers 11 are arranged in parallel.

Further, the gap 12 divides the spacer 11 into an upper spacer 111 and a lower spacer 112.

Further, when the rear plate 1 and the front plate 2 are assembled, the gap 12 corresponds to a gap between the front upper plate 21 and the front lower plate 22.

Further, the front upper plate 21 is provided with a notch 23 along the upper edge thereof, and plate lugs 24 are formed on both sides thereof.

Example two

On the basis of the first embodiment, as shown in fig. 4, the sealing strip 3 is further included, and the sealing strip 3 is integrally connected by a bottom sealing strip 31, a side sealing strip 32 and a middle sealing strip 33.

Further, the sealing strip 3 is used for plugging a gap between the rear plate 1 and the front plate 2 so as to ensure that glue liquid cannot leak out in the glue making process.

Further, the bottom seal 31 is used for sealing the gap between the bottom of the front lower plate 22 and the rear plate 1, the side seals 32 are used for sealing the gap between the rear plate 1 and the two sides of the front plate 2, and the middle seal 33 is used for sealing the gap between the bottom of the front upper plate 21 and the rear plate 1.

EXAMPLE III

In addition to the second embodiment, as shown in fig. 5, the section of the middle seal 33 is "L" or "⊥", and the positional relationship in the gel device is shown in fig. 5.

Example four

On the basis of the second or third embodiment, as shown in fig. 4, the sealing strip 3 is provided with a first through hole 34 at the junction of the side sealing strip 32 and the middle sealing strip 33.

Further, the first through hole 34 is opened in a space surrounded by the bottom seal 31, the side seals 32, and the middle seal 33, and is opened toward a gap between the front lower plate 22 and the rear plate 1 after assembly.

Further, the first through hole 34 is disposed obliquely upward from the inside to the outside.

EXAMPLE five

On the basis of the second, third or fourth embodiment, the first through hole 34 hole wall is made of hard material.

EXAMPLE six

On the basis of the first or second or third or fourth or fifth embodiment, as shown in fig. 6, the sealing device further includes a sealing block 4, and the sealing block 4 is provided with a second through hole 41.

Further, the second through hole 41 is provided at an upper center portion of the seal block 4.

EXAMPLE seven

On the basis of the sixth embodiment, as shown in fig. 6, the second through hole 41 hole wall is made of a hard material.

Example eight

On the basis of the first embodiment, the second embodiment, the third embodiment, the fourth embodiment, the fifth embodiment, the sixth embodiment and the seventh embodiment, as shown in fig. 8, an adhesive tape 6 is further included.

Further, the adhesive tape 6 is used to seal the gap between the front upper plate 21 and the front lower plate 22.

Example nine

As shown in FIGS. 1 to 8, the method for using the gel preparation apparatus for SDS-PAGE comprises the following steps:

1) the back plate 1 is horizontally placed, and the surface where the spacing sheet 11 is located faces upwards;

2) the sealing strip 3 is placed on the rear plate 1, the bottom sealing strip 31 is placed below the spacing piece 11, the side sealing strip 32 is placed on the outer side of the spacing piece 11, and the middle sealing strip 33 is placed in the gap 12 of the spacing piece 11;

3) the front upper plate 21 of the front plate 2 is placed on the upper spacing sheet 111, the front lower plate 22 is placed on the lower spacing sheet 112, and the rear plate 1 and the front plate 2 are clamped tightly;

4) separating glue is added into a gap between the front lower plate 22 and the rear plate 1, concentrated glue is added into a gap between the front upper plate 21 and the rear plate 1, and the comb 5 is inserted;

5) after the gel is formed, the sealing strip 3 is taken down, the sealing block 4 is placed in the gap 12, the adhesive tape 6 seals the gap between the front upper plate 21 and the front lower plate 22, and connecting glue is injected between the concentrated glue and the separation glue.

Although the present invention has been described with reference to the accompanying drawings, it is not intended to limit the scope of the present invention, and those skilled in the art should understand that various modifications or variations that can be made by those skilled in the art without inventive work are still within the scope of the present invention.

Claims

1. An SDS-PAGE gel preparation device, which is characterized in that: including back plate (1) and front bezel (2), back plate (1) both sides set up spacer (11) respectively, set up breach (12) on spacer (11), front bezel (2) comprise preceding upper plate (21) and preceding hypoplastron (22).

2. The SDS-PAGE gel preparation apparatus according to claim 1, further comprising a sealing strip (3), wherein the sealing strip (3) is integrally formed by a bottom sealing strip (31), a side sealing strip (32) and a middle sealing strip (33).

3. The SDS-PAGE gel preparation apparatus according to claim 2, wherein said sealing strip (3) has a first through hole (34) formed at the junction of said side sealing strip (32) and said center sealing strip (33).

4. The SDS-PAGE gel preparation apparatus according to claim 3, wherein the walls of said first through-holes (34) are made of a hard material.

5. The apparatus for SDS-PAGE according to claim 1, further comprising a sealing block (4), wherein the sealing block (4) has a second through hole (41).

6. The SDS-PAGE gel preparation apparatus according to claim 5, wherein the walls of said second through holes (41) are made of a hard material.

7. The SDS-PAGE gel preparation apparatus according to claim 1, further comprising an adhesive tape (6), wherein said adhesive tape (6) is used for sealing a gap between said front upper plate (21) and said front lower plate (22).