CN220384110U - Four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control - Google Patents
Four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control Download PDFInfo
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- CN220384110U CN220384110U CN202321572900.4U CN202321572900U CN220384110U CN 220384110 U CN220384110 U CN 220384110U CN 202321572900 U CN202321572900 U CN 202321572900U CN 220384110 U CN220384110 U CN 220384110U
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 292
- 239000007788 liquid Substances 0.000 title claims abstract description 146
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 146
- 238000004321 preservation Methods 0.000 title claims abstract description 37
- 238000003860 storage Methods 0.000 claims abstract description 228
- 238000007710 freezing Methods 0.000 claims abstract description 127
- 230000008014 freezing Effects 0.000 claims abstract description 127
- 238000007789 sealing Methods 0.000 claims abstract description 27
- 238000005138 cryopreservation Methods 0.000 claims abstract description 15
- 239000011159 matrix material Substances 0.000 claims description 48
- 229910045601 alloy Inorganic materials 0.000 claims description 11
- 239000000956 alloy Substances 0.000 claims description 11
- 229910000510 noble metal Inorganic materials 0.000 claims description 11
- 238000001816 cooling Methods 0.000 claims description 6
- 239000003292 glue Substances 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 50
- 238000000034 method Methods 0.000 description 15
- 230000008569 process Effects 0.000 description 11
- KFZMGEQAYNKOFK-UHFFFAOYSA-N isopropyl alcohol Natural products CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 10
- 230000008676 import Effects 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 235000011089 carbon dioxide Nutrition 0.000 description 6
- 230000006378 damage Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 230000000630 rising effect Effects 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000005779 cell damage Effects 0.000 description 3
- 208000037887 cell injury Diseases 0.000 description 3
- 239000002577 cryoprotective agent Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 230000002427 irreversible effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
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Abstract
The utility model provides a four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control, which comprises: an upper cover and a lower cover; the lower cover and the upper cover can be covered to form a closed storage space, and the lower cover is provided with a storage cavity; the upper cover is provided with a vent hole which is communicated with the storage cavity and is used for being connected with the program cooling device; the vent hole is provided with a sealing plug which is used for plugging the vent hole; the device also comprises a freezing storage rack, wherein the freezing storage rack is arranged in the storage cavity and comprises one or more first storage holes and one or more second storage holes; the first storage hole is used for storing a cell sample cryopreservation tube; the second storage hole is a blind hole. The device can realize free switching among cell freezing, low-temperature cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control.
Description
Technical Field
The utility model relates to a four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control.
Background
Cryopreservation refers to a technique for preserving biological samples at low temperatures (e.g., liquid nitrogen, ultra-low temperature refrigerator). Scientists have long demonstrated that cell samples of organisms can be stored in a low temperature environment for long periods of time. However, the direct exposure of cells to low temperature conditions can cause damage to the cells, such as ice crystals or solutes, which are irreversible and can affect the activity of the cells after resuscitation. Scientists have further found that adding cryoprotectants to cell samples, if stored directly in a low temperature environment, can reduce irreversible damage to cells due to the low temperature environment, but does not achieve the desired effect. Scientists further research and discover that the cell sample added with the cryoprotectant is subjected to programmed cooling, so that ice crystal or solute damage can be obviously reduced, and the resuscitating activity rate of cells is improved.
At present, an isopropyl alcohol program cooling box or a program cooling instrument is most commonly used for program cooling of a cell sample, but isopropyl alcohol belongs to a toxic solvent and is extremely volatile, so that the environment is polluted after long-term use, and the personal safety of experimental staff is threatened; the program cooling instrument is high in price, and the personal safety of experimental staff is also at great risk because of the characteristics of easy volatilization and extremely low temperature of liquid nitrogen.
Typically, a cell sample can be stored in a refrigerator at-80 ℃ for half a year, but the temperature of the cell sample can rise because things are often found in the refrigerator. If the power is off, it is more dangerous. In addition, when the cell sample is transferred from the refrigerator at-80 ℃ to the liquid nitrogen tank, the residence time outside the liquid nitrogen tank is too long without dry ice, so that the temperature of the cell sample is easily increased to cause cell destruction.
The prior art discloses a gradient program cooling cell cryopreservation box, the internal structure of which is basically nonmetallic, the cell sample volume is not large (usually 1 mL), the sample flux is small (at most 30 holes flux), and the box only has the function of realizing cell cryopreservation by adopting gradient program cooling, but can not realize free switching among cell cryopreservation, low-temperature cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control.
Disclosure of Invention
Aiming at the technical problems in the prior art, the utility model provides a four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control. The device has the following beneficial effects:
(1) The device does not use toxic solvents such as isopropanol and the like in the using process, so that the device does not pollute the environment and threaten the personal safety of experimental staff; the device does not volatilize liquid nitrogen because the device does not relate to liquid nitrogen in the procedure cooling process, so the personal safety of experimental staff can not be influenced in the process.
(2) After the temperature of the device is reduced by the program, the device can also be directly placed in a refrigerator at the temperature of minus 80 ℃ for cell preservation. In addition, after the device is cooled by a program, the device is placed in a refrigerator at the temperature of minus 80 ℃ for 2 hours, after the vent hole is sealed by the sealing plug, the device can be kept for 8 hours in a dry ice range from minus 80 ℃ to minus 40 ℃ even after power is off, so that the temperature rise caused by power off or object searching of the refrigerator at the temperature of minus 80 ℃ is not needed.
(3) When the cell sample is required to be transferred into liquid nitrogen, after the preparation work is finished, the freezing frame is only required to be taken out and put into a liquid nitrogen freezing box which is matched with the freezing frame in volume, so that the cell sample cannot stay outside the liquid nitrogen for a long time to cause the temperature rise of the cell sample to cause cell damage.
(4) When the cells need to be found out from the liquid nitrogen, the freezing frame is lifted from the liquid nitrogen, and the liquid nitrogen still remains in the second storage hole, so that the temperature rising speed of the freezing frame can be reduced, the temperature of the cell sample can be ensured, and the cells can be conveniently and conveniently taken from the liquid nitrogen.
(5) In the device, the maximum cell sample volume can reach 3.5mL (adapting to various import and domestic freezing storage pipes of 1-3.5 mL), the maximum cell sample volume can reach 169, and free switching among cell freezing storage, low-temperature cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control can be realized.
(6) The second storage holes are only used for storing liquid nitrogen when the freezing shelf is put into the liquid nitrogen or taken out from the liquid nitrogen (namely, are not used for storing the cell sample freezing tube), and all the first storage holes are arranged in a 5×5 matrix, an 8×8 matrix, a 9×9 matrix, a 13×13 matrix or a 9×12 matrix; the number of the second storage holes is 4, 5, 6, 7 or 8; in the device made of noble metal alloy, when the cells need to be found, the second storage hole is resided with liquid nitrogen after the freezing frame in the device is lifted up, so that the noble metal alloy freezing frame can be kept for more than 10min below-80 ℃.
The utility model solves the technical problems and realizes the corresponding technical effects through the following technical scheme:
the utility model provides a four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control, which comprises:
an upper cover and a lower cover; the lower cover and the upper cover can be covered to form a closed storage space, and the lower cover is provided with a storage cavity; the upper cover is provided with a vent hole which is communicated with the storage cavity and is used for being connected with a program cooling device; the vent hole is provided with a sealing plug which is used for plugging the vent hole;
the device also comprises a freezing shelf, wherein the freezing shelf is arranged in the storage cavity and comprises one or more first storage holes and one or more second storage holes; the first storage hole is used for storing a cell sample cryopreservation tube; the second storage hole is a blind hole.
When the device is used, the cell sample freezing tube needing program cooling is placed into the first storage hole of the freezing frame, the program cooling device is connected to the sealing plug of the vent hole after the sealing plug of the vent hole is pulled out for program cooling, and after the program cooling is finished, the sealing plug is plugged, and the cell sample freezing tube can be directly placed into a refrigerator at the temperature of minus 80 ℃ for cell preservation under the condition that the cell sample freezing tube is not temporarily transferred to liquid nitrogen. When the cell sample is required to be transferred into liquid nitrogen, after the preparation work is finished, the freezing frame is only required to be taken out and put into a liquid nitrogen freezing box which is matched with the freezing frame in volume, so that the cell sample cannot stay outside the liquid nitrogen for a long time to cause the temperature rise of the cell sample to cause cell damage. When the cells need to be found out from the liquid nitrogen, the freezing frame is lifted from the liquid nitrogen, and the liquid nitrogen still remains in the second storage hole, so that the temperature rising speed of the freezing frame can be reduced, the temperature of the cell sample can be ensured, and the cells can be conveniently and conveniently taken from the liquid nitrogen. Therefore, the device can realize free switching among cell freezing, low-temperature cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control.
In the utility model, the freezing storage rack can be detachably fixed with the storage cavity. Preferably, the cryopreservation shelf is adapted to the storage chamber (i.e. is placed directly inside the storage chamber without being secured by a securing member).
In the utility model, the second storage hole can be at least used for liquid nitrogen residence when the freezing frame is taken out of the liquid nitrogen.
In another preferred embodiment, the second storage hole can also be used for liquid nitrogen residence when the freezing shelf is placed in liquid nitrogen.
In another preferred embodiment, the second storage well can also be used to store a cell sample cryopreservation tube. Preferably, the first storage hole is a blind hole, and the structure of the first storage hole is the same as that of the second storage hole. The device not only can maximally utilize the storage space and improve the utilization rate of the device, but also is convenient to process and manufacture.
Preferably, all the first storage holes and all the second storage holes are arranged in a 5×5 matrix, an 8×8 matrix, a 9×9 matrix, a 13×13 matrix, or a 9×12 matrix. More preferably, the number of the second storage holes is 4, 5, 6, 7 or 8.
In another preferred embodiment, the second storage well can also be used to store a cell sample cryopreservation tube. Preferably, the structure of the first storage hole is the same as that of the second storage hole, and the difference is that the first storage hole is a through hole and the second storage hole is a blind hole. The device not only can maximally utilize the storage space and improve the utilization rate of the device, but also is convenient to process and manufacture, and can further avoid volatilization of liquid nitrogen when the cells are found, so that the cells are conveniently taken from the liquid nitrogen.
Preferably, all the first storage holes and all the second storage holes are arranged in a 5×5 matrix, an 8×8 matrix, a 9×9 matrix, a 13×13 matrix, or a 9×12 matrix. More preferably, the number of the second storage holes is 4, 5, 6, 7 or 8.
In another preferred embodiment, the second storage hole is only used for liquid nitrogen residence (i.e. not used for storing the cell sample freezing tube) when the freezing frame is put into liquid nitrogen and taken out from liquid nitrogen, and the second storage hole is arranged between the first storage holes which are partially adjacent. The first storage hole may be a blind hole; preferably, the first storage hole is a through hole.
Preferably, all the first storage holes are arranged in a 5×5 matrix, an 8×8 matrix, a 9×9 matrix, a 13×13 matrix, or a 9×12 matrix. More preferably, the number of the second storage holes is 4, 5, 6, 7 or 8. Still more preferably, the freezing shelf is made of a noble metal alloy. When the cells need to be found, liquid nitrogen resides in the second storage hole after the freezing and storing frame in the device is lifted, so that the noble metal alloy freezing and storing frame can be ensured to be kept below-80 ℃ for more than 10min.
The first storage holes are arranged in a 5×5 matrix, and the number of the second storage holes is 4.
The first storage holes are arranged in a 9×9 matrix, and the number of the second storage holes is 8.
The first storage holes are arranged in a 13×13 matrix, and the number of the second storage holes is 8.
The first storage holes are arranged in a 5×5 matrix, the size of the freezing frame is 67.5 mm×67.5 mm×25 mm, the number of the second storage holes is 4, the diameter of each first storage hole is 12.3 mm, the distance between the circle centers of the adjacent first storage holes is 13.5 mm, and the device is suitable for various inlets of 2mL and domestic freezing pipes.
The structure of the first storage hole is the same as that of the second storage hole, and the difference is that the first storage hole is a through hole and the second storage hole is a blind hole; all the first storage holes and all the second storage holes are arranged in an 8×8 matrix, and the size of the freezing storage rack is 75 mm×75 mm×25 mm; the diameters of each first storage hole and each second storage hole are 8.5 millimeters, the distance between the centers of two adjacent storage holes is 9 millimeters, the number of the second storage holes is four, the distance between the bottom end of each second storage hole and the bottom surface of the freezing storage rack is 2 millimeters, and the device is suitable for various inlets of 1mL and domestic freezing storage pipes.
Wherein the first storage holes are arranged in a matrix of 9×9, and the size of the freezing shelf is 123.65 mm× 123.65 mm×25 mm; the diameter of each first storage hole is 12.8 millimeters, the distance between the centers of the adjacent first storage holes is 13.5 millimeters, the number of the second storage holes is 8, and the device is suitable for various import and domestic freezing storage pipes with the volume of 2 mL.
The first storage holes are arranged in a matrix of 13×13, and the size of the freezing shelf is 122 mm×122 mm×20 mm; the diameter of each first storage hole is 8.5 mm, the distance between the centers of the adjacent first storage holes is 9 mm, the number of the second storage holes is 8, and the device is suitable for various 1mL import and domestic freezing storage pipes.
Wherein the first storage holes are arranged in an 8×12 matrix, and the size of the freezing shelf is 113.5 mm×77.5 mm×25 mm; the diameter of each first storage hole is 8.5 millimeters, and the distance between the centers of the circle of the adjacent first storage holes is 9 millimeters; the distance between the bottom end of each first storage hole and the bottom surface of the freezing storage rack is 2 millimeters, and the number of the second storage holes is 8.
Wherein the length and width of the device are 160 mm, the height is 90 mm, the thickness is 30 mm, and the device is suitable for various imported and domestic freezing storage pipes with the volume of 1-3.5 mL.
In another preferred embodiment, the second storage hole penetrates the freezing shelf in the thickness direction.
In another preferred embodiment, the second storage holes are uniformly distributed on the freezing shelf.
In another preferred embodiment, the second storage hole is in a cylindrical configuration.
In another preferred embodiment, the first storage hole is a blind hole.
In another preferred embodiment, the first storage hole is a through hole. When the cell is required to be found out from the liquid nitrogen, the freezing frame is lifted from the liquid nitrogen, the liquid nitrogen in the first storage hole is directly leaked back into the liquid nitrogen freezing box, and the liquid nitrogen still resides in the second storage hole, so that the temperature rising speed of the freezing frame can be reduced, the temperature of a cell sample can be ensured, and a large amount of volatilization of the liquid nitrogen is avoided, thereby conveniently taking the cell from the liquid nitrogen.
In another preferred embodiment, the first storage hole penetrates the freezing shelf in a thickness direction.
In another preferred embodiment, the first storage holes are uniformly distributed on the freezing shelf.
In another preferred embodiment, the first storage hole is in a cylindrical configuration.
In another preferred embodiment, the sealing plug is T-shaped or conical.
In another preferred embodiment, the upper cover and the lower cover are covered with an aviation heat insulation elastic glue heat preservation layer; the freezing frame is made of noble metal alloy. After the device is cooled by a program, the vent hole is sealed by a sealing plug after being placed in a refrigerator at the temperature of minus 80 ℃ for 2 hours, and the device can be kept for 8 hours in a dry ice range from minus 80 ℃ to minus 40 ℃ even after power is off, so that the temperature rise caused by power off or over finding of the refrigerator at the temperature of minus 80 ℃ is not required.
In another preferred embodiment, the lower cover includes a first lower cover body and a second lower cover body which are integrally formed;
the second lower cover body is positioned on the upper side surface of the first lower cover body; the side length of the second lower cover body is smaller than that of the first lower cover body; the inner sides of the first lower cover body and the second lower cover body are provided with the storage cavity;
the upper cover comprises a first upper cover body and a second upper cover body which are integrally structured; a first sealing cavity is formed in the first upper cover body, and a second sealing cavity is formed in the second upper cover body; the second upper cover body is arranged on one side of the first upper cover body, which is far away from the lower cover;
the first sealing cavity is matched with the outer contour of the second lower cover body.
In another preferred embodiment, the first lower cover and the second lower cover are coaxially disposed; the first seal cavity and the second seal cavity are coaxially arranged.
Compared with the prior art, the utility model has the following beneficial effects:
(1) The device does not use toxic solvents such as isopropanol and the like in the using process, so that the device does not pollute the environment and threaten the personal safety of experimental staff; the device does not volatilize liquid nitrogen because the device does not relate to liquid nitrogen in the procedure cooling process, so the personal safety of experimental staff can not be influenced in the process.
(2) After the temperature of the device is reduced by the program, the device can also be directly placed in a refrigerator at the temperature of minus 80 ℃ for cell preservation. In addition, after the device is cooled by a program, the device is placed in a refrigerator at the temperature of minus 80 ℃ for 2 hours, after the vent hole is sealed by the sealing plug, the device can be kept for 8 hours in a dry ice range from minus 80 ℃ to minus 40 ℃ even after power is off, so that the temperature rise caused by power off or object searching of the refrigerator at the temperature of minus 80 ℃ is not needed.
(3) When the cell sample is required to be transferred into liquid nitrogen, after the preparation work is finished, the freezing frame is only required to be taken out and put into a liquid nitrogen freezing box which is matched with the freezing frame in volume, so that the cell sample cannot stay outside the liquid nitrogen for a long time to cause the temperature rise of the cell sample to cause cell damage.
(4) When the cells need to be found out from the liquid nitrogen, the freezing frame is lifted from the liquid nitrogen, and the liquid nitrogen still remains in the second storage hole, so that the temperature rising speed of the freezing frame can be reduced, the temperature of the cell sample can be ensured, and the cells can be conveniently and conveniently taken from the liquid nitrogen.
(5) In the device, the maximum cell sample volume can reach 3.5mL (adapting to various import and domestic freezing storage pipes of 1-3.5 mL), the maximum cell sample volume can reach 169, and free switching among cell freezing storage, low-temperature cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control can be realized.
(6) The second storage holes are only used for storing liquid nitrogen when the freezing shelf is put into the liquid nitrogen or taken out from the liquid nitrogen (namely, are not used for storing the cell sample freezing tube), and all the first storage holes are arranged in a 5×5 matrix, an 8×8 matrix, a 9×9 matrix, a 13×13 matrix or a 9×12 matrix; the number of the second storage holes is 4, 5, 6, 7 or 8; in the device made of noble metal alloy, when the cells need to be found, the second storage hole is resided with liquid nitrogen after the freezing frame in the device is lifted up, so that the noble metal alloy freezing frame can be kept for more than 10min below-80 ℃.
Drawings
Other features, objects and advantages of the present utility model will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:
FIG. 1 is a schematic diagram of a four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control in example 1;
fig. 2 is a schematic view of the structure of the lower cover in embodiment 1;
FIG. 3 is a schematic top view of the lower cover in example 1;
FIG. 4 is a schematic side view of the lower cover of example 1;
FIG. 5 is a schematic cross-sectional view of the lower cover in example 1;
fig. 6 is a schematic view of the structure of the upper cover in embodiment 1;
FIG. 7 is a schematic top view of the upper cover in example 1;
FIG. 8 is a schematic side view of the upper cover of example 1;
FIG. 9 is a schematic cross-sectional view of the upper cover in example 1;
fig. 10 is a schematic structural diagram of a 5×5 freezing shelf in embodiment 1;
FIG. 11 is a schematic structural view of an 8X 8 freeze storage rack in example 2;
fig. 12 is a schematic structural view of a 9×9 freezing shelf in embodiment 3;
fig. 13 is a schematic structural view of a 13×13 freezing shelf in embodiment 4;
fig. 14 is a schematic structural view of an 8×12 freezing shelf in example 5;
FIG. 15 is a graph showing the temperature rise of the device of example 4 and a conventional freezing box.
In the figure:
1 is a lower cover; 101 is a first lower cover; 102 is a second lower cover; 2 is an upper cover; 201 is a first upper cover; 202 is a second upper cover; 3 is a freezing shelf; 301 is a first storage hole; 302 is a second storage hole.
Detailed Description
The present utility model will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present utility model, but are not intended to limit the utility model in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present utility model.
Example 1
The four-in-one device for programmed cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control as shown in fig. 1 to 10 comprises: an upper cover 2 and a lower cover 1; the lower cover 1 and the upper cover 2 can be covered to form a closed storage space, and the lower cover 1 is provided with a storage cavity; the upper cover 2 is provided with a vent hole which is communicated with the storage cavity and is used for being connected with a program cooling device; the vent hole is provided with a sealing plug which is used for plugging the vent hole;
the device further comprises a freezing storage rack 3, wherein the freezing storage rack 3 is arranged in the storage cavity, and the freezing storage rack 3 comprises one or more first storage holes 301 and one or more second storage holes 302; the first storage hole 301 is used for storing a cell sample cryopreservation tube; the second storage hole 302 is a blind hole.
When the device is used, the cell sample freezing tube needing program cooling is placed into the first storage hole 301 of the freezing frame 3, the program cooling device is connected to the sealing plug of the vent hole for program cooling after the sealing plug of the vent hole is pulled out, and after the program cooling is finished, the sealing plug is plugged, and the cell sample freezing tube is not transferred to liquid nitrogen temporarily, and can be directly placed into a refrigerator at the temperature of minus 80 ℃ for cell preservation. When the cell sample is required to be transferred into liquid nitrogen, after the preparation work is finished, the freezing frame 3 is only required to be taken out and put into a liquid nitrogen freezing box which is matched with the freezing frame in volume, so that the cell sample cannot stay outside the liquid nitrogen for a long time to cause the temperature rise of the cell sample to damage cells. When the cells need to be found out from the liquid nitrogen, the freezing frame 3 is lifted from the liquid nitrogen, and the liquid nitrogen still remains in the second storage hole 302, so that the speed of temperature rise of the freezing frame 3 can be reduced, the temperature of the cell sample can be ensured, and the cells can be conveniently and gently taken from the liquid nitrogen. Therefore, the device can realize free switching among cell freezing, low-temperature cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control.
Wherein the freezing shelf 3 is adapted to the storage chamber (i.e. is directly placed inside said storage chamber without being fixed by means of a fixing element).
The second storage holes 302 are only used for liquid nitrogen residence (i.e. not used for storing cell sample cryopreservation pipes) when the cryopreservation shelf 3 is placed in liquid nitrogen and taken out of the liquid nitrogen, and the second storage holes 302 are arranged between part of adjacent first storage holes 301; the first storage hole 301 is a through hole.
The first storage holes 301 are arranged in a 5×5 matrix, the size of the freezing frame 3 is 67.5 mm×67.5 mm×25 mm, the number of the second storage holes 302 is 4, the diameter of each first storage hole 301 is 12.3 mm, the distance between circle centers of adjacent first storage holes 301 is 13.5 mm, and the freezing frame 3 is suitable for various 2mL import and domestic freezing pipes.
Wherein the second storage hole 302 penetrates the freezing shelf 3 in the thickness direction; the second storage hole 302 is in a cylindrical configuration; the first storage hole 301 penetrates the freezing shelf 3 in the thickness direction; the first storage hole 301 is in a cylindrical configuration.
Wherein, the sealing plug is T-shaped.
Wherein, the upper cover 2 and the lower cover 1 are covered with aviation heat insulation elastic glue heat preservation layers; the freezing frame 3 is made of noble metal alloy. After the device is cooled by a program, the vent hole is sealed by a sealing plug after being placed in a refrigerator at the temperature of minus 80 ℃ for 2 hours, and the device can be kept for 8 hours in a dry ice range from minus 80 ℃ to minus 40 ℃ even after power is off, so that the temperature rise caused by power off or over finding of the refrigerator at the temperature of minus 80 ℃ is not required.
Wherein, the lower cover 1 comprises a first lower cover body 101 and a second lower cover body 102 which are integrally structured; the second lower cover 102 is located on the upper side of the first lower cover 101; the side length of the second lower cover body 102 is smaller than that of the first lower cover body 101; the inner sides of the first lower cover body 101 and the second lower cover body 102 are provided with storage cavities; the upper cover 2 includes a first upper cover body 201 and a second upper cover body 202 of an integral structure; the first upper cover 201 is internally provided with a first sealing cavity, and the second upper cover 202 is internally provided with a second sealing cavity; the second upper cover 202 is arranged on one side of the first upper cover 201 away from the lower cover; the first sealing cavity is matched with the outer contour of the second lower cover body 102; the first lower cover 101 and the second lower cover 102 are coaxially arranged; the first seal chamber and the second seal chamber are coaxially disposed. The device is suitable for various imported and domestic freezing storage pipes with the volume of 2 mL.
Example 2
The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control shown in fig. 11 is the same as the device of embodiment 1, except that the structure of the first storage hole 301 is the same as that of the second storage hole 302, the first storage hole 301 is a through hole, and the second storage hole 302 is a blind hole; all the first storage holes 301 and all the second storage holes 302 are arranged in an 8×8 matrix, and the size of the freezing shelf 3 is 75 mm×75 mm×25 mm; the diameter of each first storage hole 301 and the diameter of each second storage hole 302 are 8.5 mm, the distance between the centers of two adjacent storage holes is 9 mm, the number of the second storage holes 302 is four, the distance between the bottom ends of the second storage holes 302 and the bottom surface of the freezing storage rack 3 is 2 mm, and the device is suitable for various 1mL inlets and domestic freezing storage pipes.
Example 3
The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control shown in fig. 12 is the same as the device of example 1, except that the first storage holes 301 are arranged in a 9×9 matrix, and the size of the freezing frame 3 is 123.65 mm× 123.65 mm×25 mm; the diameter of each first storage hole 301 is 12.8 mm, the distance between the centers of the adjacent first storage holes 301 is 13.5 mm, the number of the second storage holes 302 is 8, and the device is suitable for various import and domestic freezing storage pipes with the volume of 2 mL.
Example 4
The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control shown in fig. 13 is the same as the device of example 1, except that the first storage holes 301 are arranged in a matrix of 13×13, and the size of the freezing frame 3 is 122 mm×122 mm×20 mm; the diameter of each first storage hole 301 is 8.5 mm, the distance between the centers of the adjacent first storage holes 301 is 9 mm, the number of the second storage holes is 8 (not shown), and the device is suitable for various import and domestic freezing storage pipes with the volume of 1 mL.
Example 5
The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control shown in fig. 14 is the same as the device of example 1, except that the first storage holes 301 are arranged in a matrix of 8×12, and the size of the freezing frame 3 is 113.5 mm×77.5 mm×25 mm; the diameter of each first storage hole 301 is 8.5 mm, and the distance between the centers of the adjacent first storage holes 301 is 9 mm; the distance between the bottom end of each first storage hole 301 and the bottom surface of the freezing storage rack 3 is 2 mm, and the number of the second storage holes is 8 (not shown), so that the device is convenient to operate by using a gun, and is suitable for 1mL of various import and domestic freezing storage pipes.
Effect example 1
Compared with the prior art, the device of the embodiments 1-5 has the following beneficial effects:
(1) The device does not use toxic solvents such as isopropanol and the like in the using process, so that the device does not pollute the environment and threaten the personal safety of experimental staff; the device does not volatilize liquid nitrogen because the device does not relate to liquid nitrogen in the procedure cooling process, so the personal safety of experimental staff can not be influenced in the process.
(2) After the temperature of the device is reduced by the program, the device can also be directly placed in a refrigerator at the temperature of minus 80 ℃ for cell preservation. In addition, after the device is cooled by a program, the device is placed in a refrigerator at the temperature of minus 80 ℃ for 2 hours, after the vent hole is sealed by the sealing plug, the device can be kept for 8 hours in a dry ice range from minus 80 ℃ to minus 40 ℃ even after power is off, so that the temperature rise caused by power off or object searching of the refrigerator at the temperature of minus 80 ℃ is not needed.
(3) When the cell sample is required to be transferred into liquid nitrogen, after the preparation work is finished, the freezing frame 3 is only required to be taken out and put into a liquid nitrogen freezing box which is matched with the freezing frame in volume, so that the cell sample cannot stay outside the liquid nitrogen for a long time to cause the temperature rise of the cell sample to damage cells.
(4) When the cells need to be found out from the liquid nitrogen, the freezing frame 3 is lifted from the liquid nitrogen, and the liquid nitrogen still remains in the second storage hole 302, so that the speed of temperature rise of the freezing frame 3 can be reduced, the temperature of the cell sample can be ensured, and the cells can be conveniently and gently taken from the liquid nitrogen.
(5) In the device, the maximum cell sample volume can reach 3.5mL (adapting to various import and domestic freezing storage pipes of 1-3.5 mL), the maximum cell sample volume can reach 169, and free switching among cell freezing storage, low-temperature cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control can be realized.
(6) The second storage holes 302 are only used for storing liquid nitrogen when the freezing shelf 3 is put into or taken out from the liquid nitrogen (i.e. not used for storing cell sample freezing tubes), and all the first storage holes 301 are arranged in a 5×5 matrix, an 8×8 matrix, a 9×9 matrix, a 13×13 matrix or a 9×12 matrix; the number of the second storage holes 302 is 4, 5, 6, 7, or 8; in the device with the freezing and storing frame 3 made of noble metal alloy, when the cells need to be found, the second storage hole 302 is resided with liquid nitrogen after the freezing and storing frame 3 in the device is lifted up, so that the noble metal alloy freezing and storing frame 3 can be kept for more than 10min below-80 ℃ (see figure 15).
The temperature rise curve of fig. 15 is obtained by detecting the cell freezing tube by embedding 30 wireless transmitting Thermo micro temperature probes. As can be seen from fig. 15, the device of the present utility model maintains a lower temperature throughout 10 minutes, thereby better protecting the cells in the cell cryopreservation vessel.
The foregoing describes specific embodiments of the present utility model. It is to be understood that the utility model is not limited to the particular embodiments described above, and that various changes and modifications may be made by one skilled in the art within the scope of the claims without affecting the spirit of the utility model.
Claims (10)
1. A four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control, comprising:
an upper cover and a lower cover; the lower cover and the upper cover can be covered to form a closed storage space, and the lower cover is provided with a storage cavity; the upper cover is provided with a vent hole which is communicated with the storage cavity and is used for being connected with a program cooling device; the vent hole is provided with a sealing plug which is used for plugging the vent hole;
the device also comprises a freezing shelf, wherein the freezing shelf is arranged in the storage cavity and comprises one or more first storage holes and one or more second storage holes; the first storage hole is used for storing a cell sample cryopreservation tube; the second storage hole is a blind hole.
2. The four-in-one device for programmed cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 1, wherein the second storage hole is at least capable of being used for liquid nitrogen residence when the freezing shelf is taken out of liquid nitrogen; the second storage hole can also be used for liquid nitrogen residence when the freezing storage rack is placed into liquid nitrogen; the second storage well can also be used to store a cell sample cryopreservation tube.
3. The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 2, wherein the first storage hole is a blind hole, and the structure of the first storage hole is the same as the structure of the second storage hole.
4. The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 2, wherein the structure of the first storage hole is the same as the structure of the second storage hole, and the difference is that the first storage hole is a through hole and the second storage hole is a blind hole.
5. The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 1, wherein the second storage hole is only used for liquid nitrogen residence when the freezing frame is put into liquid nitrogen or taken out from liquid nitrogen, and the second storage hole is arranged between part of adjacent first storage holes; the first storage hole is a blind hole.
6. The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 1, wherein the second storage holes are only used for liquid nitrogen residence when the freezing frame is put into liquid nitrogen and when the freezing frame is taken out from the liquid nitrogen, and the second storage holes are arranged between part of adjacent first storage holes; the first storage hole is a through hole.
7. The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 6, wherein the first storage holes are arranged in a 5 x 5 matrix, an 8 x 8 matrix, a 9 x 9 matrix, a 13 x 13 matrix or a 9 x 12 matrix; the number of the second storage holes is 4, 5, 6, 7 or 8.
8. The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 1, wherein the second storage hole penetrates through the freezing shelf in the thickness direction; the second storage holes are uniformly distributed on the freezing storage rack; the second storage hole is in a cylindrical structure.
9. The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 1, wherein the first storage hole is a blind hole or a through hole; the first storage hole penetrates through the freezing storage rack along the thickness direction; the first storage holes are uniformly distributed on the freezing storage rack; the first storage hole is in a cylindrical structure.
10. The four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control according to claim 1, wherein,
the sealing plug is T-shaped or conical;
the freezing storage rack is matched with the storage cavity;
the upper cover and the lower cover are covered with aviation heat insulation elastic glue heat preservation layers; the freezing frame is made of noble metal alloy;
the lower cover comprises a first lower cover body and a second lower cover body which are integrally structured; the second lower cover body is positioned on the upper side surface of the first lower cover body; the side length of the second lower cover body is smaller than that of the first lower cover body; the inner sides of the first lower cover body and the second lower cover body are provided with the storage cavity;
the upper cover comprises a first upper cover body and a second upper cover body which are integrally structured; a first sealing cavity is formed in the first upper cover body, and a second sealing cavity is formed in the second upper cover body; the second upper cover body is arranged on one side of the first upper cover body, which is far away from the lower cover;
the first sealing cavity is matched with the outer contour of the second lower cover body;
the first lower cover body and the second lower cover body are coaxially arranged; the first seal cavity and the second seal cavity are coaxially arranged.
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| CN202321572900.4U CN220384110U (en) | 2023-06-19 | 2023-06-19 | Four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control |
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| CN202321572900.4U CN220384110U (en) | 2023-06-19 | 2023-06-19 | Four-in-one device for program cooling, cell preservation, liquid nitrogen transfer and liquid nitrogen temperature control |
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