CN212414513U

CN212414513U - Magnetic sealing type vitrified tissue cryopreservation tube

Info

Publication number: CN212414513U
Application number: CN202022070299.1U
Authority: CN
Inventors: 李梅; 张帅
Original assignee: Shandong University
Current assignee: Shandong University
Priority date: 2020-09-18
Filing date: 2020-09-18
Publication date: 2021-01-29
Anticipated expiration: 2030-09-18

Abstract

The utility model provides a magnetic sealing type vitrified tissue cryopreservation tube, which belongs to the technical field of vitrified tissue preservation and comprises a tube body and a cover body detachably connected with the tube body; a permanent magnet is arranged in the tube body; a hollow cavity is arranged on the cover body, one end of the hollow cavity is closed, and the other end of the hollow cavity is a horn-shaped opening; a ball body capable of generating suction with the permanent magnet is placed in the hollow cavity; the ball body plugs the horn-shaped opening under the action of the suction force of the permanent magnet, so that the hollow cavity is not communicated with the pipe body; when the ball body overcomes the suction force and leaves the horn-shaped opening, the hollow cavity is communicated with the tube body, and the hollow cavity is provided with a pressure relief hole communicated with the outside. The utility model discloses utilize magnetism attraction control to provide safe and stable airtight space for the vitrification tissue, when guaranteeing intraductal tissue and liquid nitrogen sealed under low temperature environment, can guarantee the timely release of intraductal vaporization nitrogen again, realized effective isolation of intraductal sample and external environment, avoided the emergence that the sample that intraductal atmospheric pressure rises and causes preserves the incident.

Description

Magnetic sealing type vitrified tissue cryopreservation tube

Technical Field

The utility model relates to a vitrified tissue preserves technical field, concretely relates to magnetic seal formula vitrified tissue cryopreservation pipe.

Background

In the biomedical field, an important means for preserving biological materials such as cells is cryofreezing, which is a method of freezing cells or tissues in a liquid nitrogen tank at-196 ℃.

Vitrification is one of the research directions of the fastest development of the biological low-temperature preservation technology in recent years, overcomes the defects of gradual temperature reduction and long-time balance adopted in the conventional freezing process, and has the advantages of short operation time, simpler process, no need of a special program cooling instrument, good freezing and storing effects and the like. Vitrification is also called as ice crystal-free technology, rapid cooling is adopted in the freezing process, theoretically, the cooling speed is more than-1500 ℃/min, the formation of ice crystals is avoided to the maximum extent, the solution directly enters the glass state, and the relation between glass state solid molecules is not obviously changed compared with the liquid state, so that the extrusion damage and the freeze-thaw effect caused by the ice crystals can be avoided; meanwhile, the contact time between the cells and the cryoprotectant is reduced, and the toxicity of the cryoprotectant to the cells is reduced, so that the recovery efficiency of the vitrified cryopreserved cells is high.

The vitrification freezing and storing technology is an important method for storing cells, animal and plant tissues, embryos, ova and the like at present, provides a faster and efficient freezing mode, but has defects, and in order to avoid the safety accidents of storing tissues caused by the fact that the pressure in a tube is increased and the pressure cannot be released due to the evaporation of liquid nitrogen in a closed environment in the tube body, the traditional vitrification tissue freezing and storing tube is generally designed in an open mode and cannot meet the requirement of storing samples in an independent sealing mode.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a quick high-efficient freezing to vitrified tissue, and can realize intraductal pressure release and keep apart the magnetic seal formula vitrified tissue cryopreservation pipe of conversion to solve at least one technical problem who exists among the above-mentioned background art.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the utility model provides a magnetic seal formula vitrification tissue cryopreservation pipe, this magnetic seal formula vitrification tissue cryopreservation pipe includes:

the device comprises a pipe body and a cover body detachably connected with the pipe body;

a permanent magnet is arranged in the tube body;

the cover body is provided with a hollow cavity, one end of the hollow cavity is closed, and the other end of the hollow cavity is a horn-shaped opening;

an independent sphere capable of generating suction with the permanent magnet is placed in the hollow cavity;

the ball body plugs the horn-shaped opening under the action of the suction force of the permanent magnet, so that the hollow cavity is not communicated with the pipe body any more; when the ball body leaves the horn-shaped opening by overcoming the suction force, the hollow cavity is communicated with the tube body;

the hollow cavity is provided with a pressure relief hole communicated with the outside.

Preferably, the hollow cavity is arranged on the outer surface of the cover body.

Preferably, the thickness of the cover body is gradually reduced from the periphery to the center, an arc-shaped recess is formed on the inner surface of the cover body, and the arc-shaped recess is communicated with the horn-shaped opening. Therefore, the arc-shaped recess formed on the lower surface of the cover body is convenient for the gas in the pipe body to collect and enter the hollow cavity through the horn-shaped opening.

Preferably, the permanent magnet is arranged at the bottom of the pipe body.

Preferably, the bottom of the pipe body is provided with a blind hole, and the permanent magnet is embedded in the blind hole.

Preferably, the diameter of the ball is larger than that of the pressure relief hole.

Preferably, the diameter of the hollow cavity is larger than that of the sphere, and the diameter of the mouth of the trumpet-shaped opening is smaller than that of the sphere.

Preferably, the inner wall of the opening one end of the pipe body is provided with an internal thread, the outer wall of the cover body is provided with an external thread, and the pipe body and the cover body are detachably connected through the matching of the internal thread and the external thread.

Preferably, the outer surface of the pipe body is provided with a circumferential recess.

Preferably, the ball is made of a magnetic material.

The utility model discloses beneficial effect: the permanent magnet attracts the ball body to plug the horn-shaped opening, so that the sealing isolation of the vitrified tissue in the tube body is realized, liquefied nitrogen in the tube body is vaporized to enable the air pressure in the tube body to rise, the air pressure enables the ball body to overcome the attraction force to leave the horn-shaped opening, and gaseous nitrogen in the tube body flows out through the pressure relief hole to realize the pressure relief of the tube body. The magnetic attraction control is utilized to provide a safe and stable conditional closed space for the vitrified tissue, the sealing of the tissue in the tube and liquid nitrogen in a low-temperature environment is ensured, meanwhile, the timely release and pressure relief of vaporized nitrogen in the tube can be ensured, the effective isolation of the sample in the tube and the external environment is realized, and the occurrence of sample storage safety accidents caused by the rise of the air pressure in the tube is avoided.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a front view of a magnetic sealed vitrified tissue cryopreservation tube according to an embodiment of the present invention.

Fig. 2 is a cross-sectional structure view of the magnetic sealing type vitrified tissue cryopreservation tube according to the embodiment of the present invention.

Fig. 3 is a diagram of a kit for a magnetic sealed vitrified tissue cryopreservation tube according to an embodiment of the present invention.

Wherein: 1-a pipe body; 2-a cover body; 3-a permanent magnet; 4-a hollow cavity; 5-horn shaped opening; 6-sphere; 7-pressure relief holes; 8-arc-shaped depression; 9-blind holes; 11-circumferential recess; 12-a ramiform vector; 13-a cartridge; 14-a cross-bar; 15-connecting column; 16-a base; 17-a containment chamber; 18-pole; 19-card slot.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below by way of the drawings are exemplary only for the purpose of explaining the present invention and should not be construed as limiting the present invention.

It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

As used herein, the singular forms "a", "an", "the" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

In the description of the present specification, the terms "first", "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the description of the present specification, the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, only for convenience of description and simplification of description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present technology.

Unless expressly stated or limited otherwise, the terms "mounted," "connected," "coupled," and "disposed" are intended to be inclusive and mean, for example, that they may be fixedly coupled or disposed, or that they may be removably coupled or disposed, or that they may be integrally coupled or disposed. The specific meaning of the above terms in the present technology can be understood by those of ordinary skill in the art as appropriate.

To facilitate understanding of the present invention, the present invention will be further explained with reference to specific embodiments in conjunction with the accompanying drawings, and the specific embodiments do not constitute limitations of the embodiments of the present invention.

It will be appreciated by those skilled in the art that the drawings are merely schematic representations of embodiments and that elements shown in the drawings are not necessarily required to practice the invention.

Examples

In order to realize the sealing and pressure relief of the storage environment in the process of freezing the vitrified tissues, the embodiment provides a magnetic sealing type vitrified tissue freezing and storing tube, a horn-shaped opening is blocked by a permanent magnet attraction ball body, the vitrified tissues in a tube body are sealed and isolated, the liquefied nitrogen in the tube body is vaporized to enable the air pressure in the tube body to rise, the air pressure enables the ball body to overcome the attraction force to leave the horn-shaped opening, and the gaseous nitrogen in the tube body flows out through a pressure relief hole to realize the pressure relief of the tube body.

FIG. 1 is a front view of the structure of the vitreous tissue cryopreservation tube in this embodiment. As shown in fig. 1, the magnetic sealed type vitrified tissue cryopreservation tube includes a tube body 1 and a cover body 2 detachably connected to the tube body 1, liquefied nitrogen is used as a cryopreservation medium in the tube body 1, and the vitrified tissue is cryopreserved in the tube body 1. For example, the cover body 2 and the tube body 1 are detached and separated, liquefied nitrogen is filled in the tube body 1, the vitrified tissue is put in, and the cover body 2 is connected to the tube body 1, so that the frozen preservation of the vitrified tissue can be realized.

FIG. 2 is a sectional view showing the structure of the vitreous tissue cryopreservation tube in this embodiment. As shown in fig. 2, in order to release the gaseous nitrogen stored in the tube, a release hole 7 communicating with the outside is provided in the lid body 2, the release hole 7 communicates with the tube, and when the liquid nitrogen in the tube is vaporized to increase the pressure in the tube 1, the gaseous nitrogen can flow out of the outside through the release hole 7, thereby releasing the pressure in the internal environment of the tube 1.

In order to ensure the sealed preservation environment of the vitrified tissue in the tube body 1 with the pressure relief completed, the tube body 1 with the pressure relief completed is no longer communicated with the pressure relief hole 7, in this embodiment, the pressure relief hole 7 is arranged on the hollow cavity 4 on the upper surface of the cover body 2, and the pressure relief hole 7 is communicated with the inside of the tube body 1 through the hollow cavity 4. Meanwhile, the permanent magnet 3 is arranged on the tube body 1, the hollow cavity 4 is internally provided with an independent sphere 6 which can generate suction with the permanent magnet 3, one end of the hollow cavity 4 is closed, and the other end of the hollow cavity is provided with a horn-shaped opening 5. When the pressure release is not needed, the ball body 6 in the hollow cavity 4 plugs the horn-shaped opening 5 under the attraction of the permanent magnet 3, even if the tube body 1 is not communicated with the pressure release hole 7 any more, the sealed storage environment of the vitrified tissue in the tube body 1 is ensured.

As shown in fig. 2, in this embodiment, in order to communicate the hollow cavity 4 with the inside of the tube body 1 through the trumpet-shaped opening 5, an arc-shaped recess 8 is disposed on the lower surface of the cover body 2, the arc-shaped recess 8 is formed by gradually decreasing the thickness of the cover body 2 from the periphery to the center, and the center of the arc-shaped recess 8 is communicated with the trumpet-shaped opening 5. The flared opening 5 is open toward the inside of the tube 1, i.e., the upper diameter of the flared opening 5 is larger than the lower diameter. The arc-shaped recess formed on the lower surface of the cover body is convenient for the gas in the pipe body to collect and enter the hollow cavity through the trumpet-shaped opening.

In this embodiment, in order to make the ball 6 move smoothly in the hollow cavity 4, the diameter of the hollow cavity 4 is set to be larger than the diameter of the ball 6, and in order to make the ball block the trumpet-shaped opening 5 and prevent the ball 6 from falling into the tube body 1, the diameter of the mouth of the trumpet-shaped opening 5 is set to be smaller than the diameter of the ball 6.

As shown in fig. 2, in order to stably provide the attraction force to the sphere 6 for the permanent magnet 3, the permanent magnet 3 is disposed at the bottom of the tube body 1, so that the permanent magnet 3 is directly opposite to the hollow cavity 4 on the cover body 2 at the top of the tube body 1, the hollow cavity 4 is cylindrical, the tube body 1 is also cylindrical, and the hollow cavity 4 is located at the center of the cover body 2 and is coaxial with the tube body 1.

In the present embodiment 1, the permanent magnet 3 is a sheet-like, and the spherical body 6 is made of a magnetic material, or made of iron. The sheet-like permanent magnet may generate an attractive force to the spherical body 6 made of a magnetic material or the spherical body 6 made of iron.

The lamellar permanent magnet is embedded in the blind hole 9 in the bottom pipe wall of the pipe body 1. In this embodiment, in order to realize the connection of dismantling of body 1 and lid 2 the inner wall of the opening one end of body 1 is equipped with the internal thread, be equipped with the external screw thread on the outer wall of lid 2, body 1 with lid 2 passes through the internal thread with the connection can be dismantled in the cooperation realization of external screw thread.

As shown in fig. 2, a screw hole is formed in the arc-shaped recess 8 of the cap 2, a branch-shaped carrier 12 of vitrified tissue can be attached to the cap 2 through the screw hole, and a circumferential recess 11 is formed in the outer surface of the tube body 1. The circumferential recess 11 can be used for clamping the tube body 1 with a clamping tool.

As shown in fig. 1 and 2, in another embodiment of the present invention, when preserving vitrified tissue, firstly using the accessory tool to clamp the cover 2, the internal magnetic pole 18 of the accessory tool attracts the sphere 6 in the hollow cavity 4 to the top of the hollow cavity, after the tissue is fixed in the liquid nitrogen, the hollow cavity 4 and the tube 1 are filled with liquid nitrogen, using the clamping tool to clamp the tube 1, using the accessory tool to screw the cover 2, removing the accessory tool, attracted by the permanent magnet on the tube 1, the sphere 6 falls to the bottom of the hollow cavity 4, blocking the trumpet-shaped opening 5, at this time, freezing the sealed environment of the tube is completed. When the micro-positive pressure formed by the evaporation of the liquid nitrogen in the tube body 1 reaches a certain degree, the ball body is jacked up to release the pressure.

As shown in fig. 3, the kit may have the following structure:

the cross rod 14 is connected with a connecting column 15, the connecting column 15 is connected with a base 16, and the base 16 is provided with an accommodating cavity 17 into which the hollow cavity 4 on the cover body 2 can be inserted; clamping grooves 19 for clamping the clamping blocks 13 on two sides of the hollow cavity 4 on the cover body 2 are formed in two sides of the accommodating cavity 17, and magnetic poles 18 are arranged at the top end of the accommodating cavity 17. The hollow cavity 4 on the cover body 2 and the clamping blocks 13 on the two sides are respectively inserted into the accommodating cavity 17 and the clamping grooves 19, and the cover body 2 and the pipe body 1 can be screwed by screwing the cross rod 14 to realize threaded connection.

To sum up, in the magnetic sealing type vitrified tissue cryopreservation tube of the embodiment of the present invention, the ball 6 blocks the trumpet-shaped opening 5 under the suction force of the permanent magnet 3, so that the hollow cavity 4 is not communicated with the tube body 1; when the ball 6 leaves the trumpet-shaped opening 5 against the suction force, the hollow cavity 4 communicates with the tube body 1. The permanent magnet 3 attracts the ball body 6 to block the horn-shaped opening, so that the sealing isolation of the vitrified tissue in the tube body 1 is realized, liquefied nitrogen in the tube body 1 is vaporized to enable the air pressure in the tube body to rise, the air pressure enables the ball body 6 to overcome the attraction force to leave the horn-shaped opening 5, and the gaseous nitrogen in the tube body 1 flows out through the pressure relief hole 7, so that the pressure relief of the tube body is realized.

The magnetic sealing type vitrified tissue cryopreservation tube utilizes magnetic attraction control to provide a safe and stable conditional sealed space for vitrified tissues, ensures the sealing of the tissues in the tube and liquid nitrogen in a low-temperature environment, and simultaneously can ensure the timely release and pressure relief of vaporized nitrogen in the tube, realizes the effective isolation of samples in the tube from the external environment, and avoids the occurrence of sample preservation safety accidents caused by the rise of the air pressure in the tube.

In clinical application and the preservation of closed samples, the dilemma that the samples are required to be isolated and programmed freezing has to be selected is solved, and more vitrification freezing use scenes are expanded. Solves the problem that the vitrification freezing can not meet the sample isolation requirement of the law and principle documents for a long time. Under the condition of meeting the requirement of closed preservation, the time for sample preservation is greatly compressed by using vitrification freezing. And the application of the method to the vitrification freezing and sealing preservation of more kinds of samples can be expanded according to the difference of the branch vein-shaped carriers.

The above description is only a preferred embodiment of the present disclosure and is not intended to limit the present disclosure, and various modifications and changes may be made to the present disclosure by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present disclosure should be included in the protection scope of the present disclosure.

Although the present disclosure has been described with reference to the accompanying drawings, it is not intended to limit the scope of the present disclosure, and it should be understood by those skilled in the art that various modifications and changes may be made without inventive changes in the technical solutions disclosed in the present disclosure.

Claims

1. A magnetically sealed vitrified tissue cryopreservation tube comprising:

the device comprises a pipe body (1) and a cover body (2) detachably connected with the pipe body (1);

the permanent magnet (3) is arranged on the tube body (1);

a hollow cavity (4) is arranged on the cover body (2), one end of the hollow cavity (4) is closed, and the other end of the hollow cavity (4) is provided with a horn-shaped opening (5);

an independent sphere (6) capable of generating suction with the permanent magnet (3) is arranged in the hollow cavity (4);

and the hollow cavity (4) is provided with a pressure relief hole (7) communicated with the outside.

2. The magnetically sealed vitrified tissue cryopreservation tube of claim 1 wherein: the hollow cavity (4) is arranged on the outer surface of the cover body (2).

3. The magnetically sealed vitrified tissue cryopreservation tube of claim 2 wherein: the thickness of lid (2) is by reducing gradually to the center all around the internal surface of lid (2) forms arc sunken (8), arc sunken (8) with loudspeaker form opening (5) communicate with each other.

4. The magnetically sealed vitrified tissue cryopreservation tube of claim 1 wherein: the permanent magnet (3) is arranged at the bottom of the pipe body (1).

5. The magnetically sealed vitrified tissue cryopreservation tube of claim 4 wherein: the bottom of body (1) is equipped with blind hole (9), permanent magnet (3) embedded in blind hole (9).

6. The magnetically sealed vitrified tissue cryopreservation tube of claim 5 wherein: the diameter of the ball body (6) is larger than that of the pressure relief hole (7).

7. The magnetically sealed vitrified tissue cryopreservation tube of claim 6 wherein: the diameter of the hollow cavity (4) is larger than that of the sphere (6), and the diameter of the opening of the horn-shaped opening (5) is smaller than that of the sphere (6).

8. The magnetically sealed vitrified tissue cryopreservation tube of claim 1 wherein: the inner wall of the opening one end of the pipe body (1) is provided with an internal thread, the outer wall of the cover body (2) is provided with an external thread, and the pipe body (1) and the cover body (2) are detachably connected through the matching of the internal thread and the external thread.

9. The magnetically sealed vitrified tissue cryopreservation tube of any of claims 1-8 wherein: the outer surface of the pipe body (1) is provided with a circumferential recess (11).

10. The magnetically sealed vitrified tissue cryopreservation tube of any of claims 1-8 wherein: the ball (6) is made of a magnetic material.