CN216722876U - Refrigeration system - Google Patents

Abstract

The utility model provides a refrigeration system, which comprises a refrigeration transfer device and a negative pressure vaporization device; the freezing transfer device is detachably connected with the negative pressure vaporization device; the freezing transfer device is provided with an inner cavity, and the inner cavity is used for accommodating a refrigerant; when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporization device is used for pumping negative pressure to the inner cavity so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling temperature of the refrigerant under the external atmospheric pressure. Therefore, negative pressure is pumped into the inner cavity of the freezing transfer device through the negative pressure vaporization device, the boiling point of the refrigerant is reduced, the temperature of the refrigerant contained in the inner cavity of the freezing transfer device is reduced, and the cooling rate of biological tissues is improved. Is beneficial to vitrification of the cryoprotectant, thereby reducing the concentration of the cryoprotectant in the frozen liquid, reducing the toxicity and damage of refrigeration and improving the quality of refrigerated biological tissues.

Description

Refrigeration system

Technical Field

The utility model relates to the technical field of medical instruments, in particular to a freezing system.

Background

In the field of human assisted reproduction, low-temperature cryopreservation of embryos and ova is an important component, and a vitrification freezing method is a commonly used embryo cryopreservation technology at present. According to the embryo cryopreservation technology, on one hand, cells and tissues are treated by using a high-concentration cell protection solution so as to improve the glass transition temperature, and on the other hand, more efficient vitrification is realized by improving the cooling rate. Among the specific methods for achieving vitrification, the Cryotop method is widely used due to the fact that the operation is simple, the obtained freezing rate is high, and the survival rate and the development rate of cells after vitrification preservation are high.

The Cryotop method was the high-speed freezing method proposed by Kuwayama in 2005 according to the principle of minimizing the volume of a solution. The carrier of this solution is made by attaching a thin plastic strip to a plastic handle. The operation is finished under a stereomicroscope, firstly, a glass capillary tube with the inner diameter slightly larger than the diameter of a cell is used for loading the oocyte on a plastic carrier, then the capillary tube is used for absorbing the redundant freezing protection liquid around the oocyte by utilizing the capillary tube principle, so that the oocyte is only covered by a thin liquid film, and then the plastic carrier carrying the oocyte is inserted into liquid nitrogen for long-term storage in the liquid nitrogen. The method has the cooling rate of 12,000 +/-1,500K/min. However, this method has problems such as a low cell cooling rate, which results in the use of a cryoprotectant at a high concentration, high cytotoxicity, and the inability to preserve cells of larger diameters.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a freezing system to solve a series of problems caused by insufficient cooling rate of the existing biological tissue freezing and preservation.

To solve the above technical problem, the present invention provides a refrigeration system, comprising: a refrigeration transfer device and a negative pressure vaporization device; the freezing transfer device is detachably connected with the negative pressure vaporization device; the freezing transfer device is provided with an inner cavity, and the inner cavity is used for accommodating a refrigerant;

when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporization device is used for pumping negative pressure to the inner cavity so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling temperature of the refrigerant under the external atmospheric pressure.

Optionally, in the refrigeration system, the refrigeration transfer device includes a container assembly and a cover assembly, and the cover assembly is openably and closably connected to the container assembly; the cover assembly, when connected to the container assembly, encloses the interior cavity.

Optionally, in the refrigeration system, the refrigeration transfer device further includes a first connection assembly, the first connection assembly is connected to the container assembly and is communicated with the inner cavity, and a movable end of the first connection assembly is used for being connected to the negative pressure vaporization device;

optionally, in the refrigeration system, the first connection assembly has a shutoff valve;

when the refrigeration transfer device is connected with the negative pressure vaporization device, the stop valve is communicated;

and when the refrigeration transfer device is separated from the negative pressure vaporization device, the stop valve is closed.

Optionally, in the refrigeration system, the refrigeration transfer device further includes a discharge valve, and the discharge valve is connected to the container assembly and is communicated with the inner cavity;

when the refrigeration transfer device is separated from the negative pressure vaporization device and the pressure in the inner cavity does not exceed a preset pressure, the discharge valve is closed;

when the refrigeration transfer device is separated from the negative pressure vaporization device and the pressure in the inner cavity exceeds the preset pressure, the discharge valve is conducted, the pressure in the inner cavity is relieved, and the discharge valve is closed until the pressure in the inner cavity does not exceed the preset pressure; wherein the predetermined pressure is not less than the ambient atmospheric pressure.

Optionally, in the refrigeration system, the container assembly comprises: the inner container, the first heat preservation sleeve and the subcooler; the cover assembly includes: sealing the subcooler;

the first heat-preserving sleeve is sleeved outside the inner container; the first heat-preserving sleeve and the inner container are contained in the subcooler together; the subcooler sealing cover can be connected with the subcooler in a sealing way in an opening and closing way.

Optionally, in the refrigeration system, the container assembly further includes a shock pad, and the shock pad is located at the bottom outside the inner container and is abutted against the inner container and the subcooler respectively.

Optionally, in the refrigeration system, the container assembly further comprises: the first shell and the second heat-insulating sleeve; the cover assembly further includes: a second shell and a third insulating sleeve; the first shell is used for being connected with the second shell in a matching manner;

the second heat-insulating sleeve is sleeved outside the subcooler; the second heat-preserving sleeve and the subcooler are contained in the first shell together; the subcooler sealing cover is connected with the second shell through the third heat-preserving sleeve.

Optionally, in the refrigeration system, the negative pressure vaporization device includes: the negative pressure pump and a second connecting component connected with the negative pressure pump; the negative pressure pump passes through the second coupling assembling with the inner chamber intercommunication is used for right the inner chamber is taken out the negative pressure.

Optionally, in the refrigeration system, the negative pressure vaporization device further includes: an air-wet vaporizer and/or bacteriostatic filter; the air wet vaporizer and/or the bacteriostatic filter are/is arranged between the negative pressure pump and the second connecting component.

Optionally, in the refrigeration system, the negative pressure vaporization device further includes: a fourth housing; the negative pressure pump and the second connecting assembly are contained in the fourth shell; the fourth shell is connected with the first shell of the freezing transfer device in a matching way.

Optionally, in the refrigeration system, the refrigeration system further includes: interaction means and/or parameter prompt means;

the interaction device is arranged on the negative pressure vaporization device; the interaction device is used for interactively inputting preset temperature parameters, and the negative pressure vaporization device is used for pumping negative pressure to the inner cavity according to the preset temperature parameters so as to keep the temperature of the refrigerant contained in the inner cavity in a temperature interval corresponding to the preset temperature parameters;

the parameter prompting device is arranged on the freezing transfer device; the parameter prompting device is used for acquiring and prompting at least one of positioning information of the freezing transfer device, the temperature of the refrigerant contained in the inner cavity, the pressure of the refrigerant and the liquid level of the refrigerant.

In summary, the present invention provides a refrigeration system comprising: a refrigeration transfer device and a negative pressure vaporization device; the refrigeration transfer device is detachably connected with the negative pressure vaporization device; the freezing transfer device is provided with an inner cavity, and the inner cavity is used for accommodating a refrigerant; when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporization device is used for pumping negative pressure to the inner cavity so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling temperature of the refrigerant under the external atmospheric pressure.

So the configuration, through negative pressure vaporization device to freezing transfer device's inner chamber take out the negative pressure, reduced the boiling point of refrigerant to make the temperature of the refrigerant of holding in freezing transfer device's the inner chamber reduce, improved the cooling rate to biological tissue. Therefore, on one hand, the vitrification of the cryoprotectant is facilitated, so that the concentration of the cryoprotectant in the frozen liquid can be reduced, the refrigeration toxicity and damage are reduced, and the quality of the refrigerated biological tissues is improved. On the other hand, the limitation on the size of the stored biological tissue is reduced, and a larger size of the biological tissue can be stored, which leads to a wider storage range. On the other hand, the freezing transfer device and the negative pressure vaporization device are separable, when the freezing transfer device and the negative pressure vaporization device are combined, the negative pressure vaporization device can pump negative pressure to the inner cavity of the freezing transfer device according to required pressure, so that the preservation temperature can be accurately controlled, and the whole freezing system can be used as a long-term storage system for vitrification freezing preservation; when the refrigeration transfer device is separated from the negative pressure vaporization device, the low-temperature refrigerant contained in the refrigeration transfer device can keep low temperature for a certain time, and can be used for transferring and transferring biological tissues.

Drawings

It will be appreciated by those skilled in the art that the drawings are provided for a better understanding of the utility model and do not constitute any limitation to the scope of the utility model. Wherein:

FIG. 1 is a front view of a refrigeration system according to an embodiment of the present invention;

FIG. 2 is a side view of a refrigeration system of an embodiment of the present invention;

FIG. 3 is a top view of a refrigeration system according to an embodiment of the present invention;

FIG. 4 is a front view of a freeze transfer device according to an embodiment of the present invention;

FIG. 5 is a side view of a freeze transfer device according to an embodiment of the present invention;

FIG. 6 is a top view of a freeze transfer device according to an embodiment of the present invention;

FIG. 7 is a front view of a negative pressure vaporizing apparatus according to an embodiment of the present invention;

FIG. 8 is a side view of a negative pressure vaporization device of an embodiment of the present invention;

FIG. 9 is a top view of a negative pressure vaporizing apparatus of an embodiment of the present invention;

FIG. 10 is a rear view of the negative pressure vaporizing apparatus of the embodiment of the present invention;

fig. 11 is an exploded view in front elevation of a refrigeration system in accordance with an embodiment of the present invention;

fig. 12 is a side exploded view of a refrigeration system in accordance with an embodiment of the present invention;

FIG. 13 is a schematic view of a container assembly and a cover assembly according to an embodiment of the present invention;

FIG. 14 is an exploded view of a container assembly and a cap assembly in accordance with an embodiment of the present invention;

FIG. 15 is an exploded view in front elevation of a negative pressure vaporizing apparatus according to an embodiment of the present invention;

FIG. 16 is a side exploded view of a negative pressure vaporizing device according to an embodiment of the present invention;

fig. 17 is a partially exploded view of a negative pressure vaporizing apparatus according to an embodiment of the present invention.

In the drawings:

1-a refrigeration transfer device; 11-a container assembly; 111-inner container; 112-a first insulating sleeve; 113-a subcooler; 114-a shock pad; 115-a first housing; 1151-a handle; 12-a cover assembly; 121-sealing cover of subcooler; 1211-sealing ring; 122-a second housing; 1221-a handle; 13-a first connection assembly;

2-negative pressure vaporizing device; 21-a negative pressure pump; 22-a second connection assembly; 23-an empty wet vaporizer; 24-a bacteriostatic filter; 25-a fourth housing;

3-an interaction device; 4-a parameter prompting device; 41-display screen; 42-function switch button; 5-control device.

Detailed Description

To further clarify the objects, advantages and features of the present invention, a more particular description of the utility model will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings. It is to be noted that the drawings are in greatly simplified form and are not to scale, but are merely intended to facilitate and clarify the explanation of the embodiments of the present invention. Further, the structures illustrated in the drawings are often part of actual structures. In particular, the drawings may have different emphasis points and may sometimes be scaled differently.

As used in this application, the singular forms "a", "an" and "the" include plural referents, the term "or" is generally employed in a sense including "and/or," the terms "a" and "an" are generally employed in a sense including "at least one," the terms "at least two" are generally employed in a sense including "two or more," and the terms "first", "second" and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first," "second," or "third" may explicitly or implicitly include one or at least two of that feature, "one end" and "the other end," and "proximal end" and "distal end" generally refer to the corresponding two parts, including not only the endpoints. Furthermore, as used herein, the terms "mounted," "connected," and "disposed" on another element should be construed broadly and generally merely indicate that a connection, coupling, fit, or drive relationship exists between the two elements, and a connection, coupling, fit, or drive relationship between the two elements, whether direct or indirect through intervening elements, should not be construed as indicating or implying any spatial relationship between the two elements, i.e., an element may be located in any orientation within, outside, above, below, or to one side of another element unless the content clearly indicates otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations. Moreover, directional terminology, such as above, below, up, down, upward, downward, left, right, etc., is used with respect to the exemplary embodiments as they are shown in the figures, with the upward or upward direction being toward the top of the corresponding figure and the downward or downward direction being toward the bottom of the corresponding figure.

The utility model aims to provide a freezing system to solve a series of problems caused by insufficient cooling rate of the existing biological tissue freezing and preservation.

The following description refers to the accompanying drawings.

The inventors found that, when a specific refrigerant is used to cryopreserve biological tissue (such as biological tissue of embryo or cell), the specific refrigerant has a specific temperature under normal conditions, and in an ambient pressure environment (which means around a standard atmospheric pressure), for example, liquid nitrogen, since the temperature of the ambient temperature environment is higher than the boiling point of liquid nitrogen, the liquid nitrogen cannot be completely in an ideal adiabatic environment and absorbs heat from the external environment, so that the liquid nitrogen is at the boiling point temperature in an equilibrium state, and a small amount of liquid nitrogen is vaporized while the liquid nitrogen is maintained at the ambient pressure boiling point temperature of approximately-196 ℃. Where its cooling rate for a particular size of biological tissue is known. If different refrigerants are replaced to improve the cooling rate, the use cost can be greatly improved by adopting the refrigerant with lower temperature, such as liquid helium.

The inventors have further found that liquid nitrogen can be kept in a liquid state as low as-210 ℃ according to a nitrogen three-phase diagram, and if the temperature of the liquid nitrogen is lowered, the liquid nitrogen below the boiling temperature of the liquid nitrogen is called supercooled liquid nitrogen. It will be appreciated that the use of subcooled liquid nitrogen increases the rate of cooling of the biological tissue. Therefore, on one hand, the vitrification of the cryoprotectant is facilitated, so that the concentration of the cryoprotectant in the frozen liquid can be reduced, the refrigeration toxicity and damage are reduced, and the quality of the refrigerated biological tissue is improved. On the other hand, the limitation on the size of the stored biological tissue is reduced, and a larger size of the biological tissue can be stored, which leads to a wider storage range. Of course, extending to other refrigerants such as liquid carbon dioxide or liquid helium, subcooled liquid carbon dioxide or subcooled liquid helium can achieve lower temperatures than liquid carbon dioxide or liquid helium at the original boiling point temperature, effectively improving the refrigeration efficiency with this type of refrigerant. In the industrial age, it is not difficult to obtain a supercooled refrigerant at a lower temperature than a liquid refrigerant of the original boiling point temperature. However, in some small scale or portable applications, the use of super cooled refrigerants is limited because bulky refrigeration units are often fixed and inconvenient to handle. In particular, in some cases where it is necessary to transport biological tissues by freezing, it is difficult to apply supercooled refrigerants produced in refrigeration equipment such as factories, which have been heated to a boiling point due to heat absorption when being transported to a freezing and transporting apparatus.

Referring to fig. 1 to fig. 3, based on the above research, an embodiment of the present invention provides a refrigeration system, which includes: a refrigeration relay device 1 and a negative pressure vaporization device 2; the freezing transfer device 1 is detachably connected with the negative pressure vaporization device 2; the freezing transfer device 1 is provided with an inner cavity, and the inner cavity is used for accommodating a refrigerant; when the refrigeration transfer device 1 is connected with the negative pressure vaporization device 2, the inner cavity is communicated with the negative pressure vaporization device 2; the negative pressure vaporization device 2 is used for pumping negative pressure to the inner cavity, so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling temperature of the refrigerant under the external atmospheric pressure.

The boiling point temperature of a specific refrigerant can be reduced by pumping negative pressure. Therefore, if negative pressure is pumped to the refrigerant contained in the inner cavity of the freezing transfer device 1, the temperature of the refrigerant contained in the inner cavity is lower than the boiling point temperature of the refrigerant under the external atmospheric pressure, and the super-cooled refrigerant can be prepared. Therefore, although a part of refrigerant is lost, the negative pressure vaporizing device 2 with smaller volume can be used without arranging a refrigeration appliance with huge volume, so that the volume of the whole refrigerating system is effectively reduced, and the refrigerating system is convenient to carry, move and use. When the refrigeration relay device 1 is connected to the negative pressure vaporization device 2, the negative pressure vaporization device 2 can maintain the refrigerant in the refrigeration relay device 1 in a supercooled state by pumping negative pressure to the inner cavity of the refrigeration relay device 1. Further, the refrigeration relay device 1 and the negative pressure vaporization device 2 are separable, after the refrigeration relay device 1 and the negative pressure vaporization device 2 are separated, the inner cavity of the refrigeration relay device 1 can be restored to the external atmospheric pressure, and meanwhile, the contained supercooling refrigerant can be maintained in a supercooling state for a period of time, so that the possibility is created for the transportation of the refrigeration relay device 1. It can be understood that, since the negative pressure vaporization device 2 pumps negative pressure to the inner cavity of the freezing and transferring device 1, gaseous refrigerant will be pumped out, and the gaseous refrigerant will be directly discharged to the outside, therefore, the refrigerant should be selected from a kind that is not polluting to the environment, and the refrigerant includes, but is not limited to, nitrogen, carbon dioxide, helium, and the like.

An exemplary embodiment of the refrigeration system will be described below with reference to fig. 1 to 17. It is to be understood that the illustrations of fig. 1-17 are merely exemplary of a refrigeration system and are not limiting of the refrigeration system.

Referring to fig. 4 to 6 and 11 to 14, the freezing and relay apparatus 1 includes: a container assembly 11 and a cover assembly 12, wherein the cover assembly 12 is connected with the container assembly 11 in an openable and closable manner; when the cover assembly 12 is coupled to the container assembly 11, the interior cavity is closed.

Optionally, the container assembly 11 comprises: an inner container 111, a first heat preservation sleeve 112 and a subcooler 113; the cover assembly 12 includes: a subcooler sealing cap 121; the inner container 111 is used for accommodating the refrigerant; the first heat-preserving sleeve 112 is sleeved outside the inner container 111; the first heat-preserving sleeve 112 and the inner container 111 are contained in the subcooler 113 together; the subcooler sealing cover 121 is connected with the subcooler 113 in a sealing way in an opening and closing way.

In an alternative example, the inner container 111 is a double-layer vacuum stainless steel barrel, the upper end of the inner container 111 is open, the inner container is used for containing refrigerant, and the inner surface and/or the outer surface of the inner container 111 has a silver coating to reduce radiation heat dissipation. The first heat-preserving cover 112 is made of ethylene-vinyl acetate copolymer (EVA) and is sleeved outside the inner container 111 for reducing heat exchange between the refrigerant in the inner container 111 and the outside. The supercooler 113 is a barrel made of Polyoxymethylene (POM) material, the upper end of the supercooler 113 is open, and the inner container 111 enclosing the first heat-insulating jacket 112 can be loaded into the supercooler 113 from the open end of the supercooler 113. The supercooler sealing cover 121 can seal the open end of the supercooler 113 so that the inside of the supercooler 113 forms a relatively closed inner chamber. Therefore, the negative pressure can be pumped to the inner cavity through the negative pressure vaporizing device 2.

Optionally, the sealing cap 121 of the subcooler is provided with a sealing ring 1211, the sealing ring 1211 may be a silica gel sealing ring, for example, and is adapted to the shape of the open end of the subcooler 113, and the sealing cap 121 of the subcooler can be connected to the open end of the subcooler 113 through the sealing ring 1211 in a sealing manner. Preferably, the container assembly 11 further includes a shock absorbing pad 114, the shock absorbing pad 114 is accommodated in the subcooler 113 and located at the bottom outside the inner container 111, and the shock absorbing pad 114 is respectively connected with the inner container 111 and the subcooler 113 in an abutting manner, and can absorb the shock of the freezing transfer device 1 in the transfer process, reduce the shock of the subcooler 113, and thereby reduce the loss of the refrigerant. The material of the shock absorbing pad 114 may be, for example, foamed rubber.

Further, the container assembly 11 further includes: a first case 115 and a second insulating jacket (located inside the first case 115 and not shown); the cover assembly 12 further includes: a second case 122 and a third insulating jacket (located inside the second case 122 and not shown); the first housing 115 is fittingly connected to the second housing 122; the second heat-insulating sleeve is sleeved outside the subcooler 113; the second insulating jacket and the subcooler 113 are contained together in the first shell 115; the subcooler sealing cover 121 is connected with the second housing 122 through the third insulating sleeve. Preferably, the first housing 115 and the second housing 122 are connected by a snap fit.

In an alternative example, the first housing 115 and the second housing 122 are made of Acrylonitrile Butadiene Styrene (ABS) material, the second insulating sheath and the third insulating sheath are made of EVA, and the second insulating sheath is disposed outside the subcooler 113 to reduce heat exchange between the inside and the outside of the subcooler 113. The third insulating jacket is used for reducing heat exchange between the subcooler 113 and the outside through the subcooler sealing cover 121. The first housing 115 and the second housing 122 of the ABS have better mechanical properties, impact resistance, and are suitable for transit and transportation. Optionally, the first housing 115 has a handle 1151, the handle 1151 of the first housing 115 is convenient for carrying, and the second housing 122 has a handle 1221, the handle 1221 being convenient for opening the sealing cover 121 of the subcooler.

Optionally, the freezing transit device 1 further includes a first connection assembly 13, the first connection assembly 13 is connected to the container assembly 11, for example, the first connection assembly 13 is disposed at the bottom of the container assembly 11 and is communicated with the inner cavity, and a movable end of the first connection assembly 13 is used for being communicated with the negative pressure vaporization device 2. Further, the first connection assembly 13 has a shutoff valve; when the refrigeration transfer device 1 is connected with the negative pressure vaporization device 2, the stop valve is conducted, so that the inner cavity is communicated with the negative pressure vaporization device 2 through the first connecting assembly 13; when the refrigeration transit device 1 is separated from the negative pressure vaporization device 2, the stop valve is closed. The first connection module 13 mainly serves as a connection port with the negative pressure vaporizing device 2. In an exemplary embodiment, the first connecting component 13 includes a blind-mate interface, which can be quickly and fittingly plugged into the corresponding second connecting component 22 of the negative pressure vaporizing device 2. In one example, the shut-off valve may be a solenoid valve or a manually operated valve, for example.

Optionally, the freezing and transferring device 1 further includes a discharge valve, and the discharge valve is connected to the container assembly 11 and is communicated with the inner cavity; when the refrigeration transfer device 1 is separated from the negative pressure vaporization device 2 and the pressure in the inner cavity does not exceed a preset pressure, the discharge valve is closed; when the refrigeration transfer device 1 is separated from the negative pressure vaporization device 2 and the pressure in the inner cavity exceeds the preset pressure, the discharge valve is conducted, the pressure in the inner cavity is relieved, and the discharge valve is closed until the pressure in the inner cavity does not exceed the preset pressure; wherein the predetermined pressure is not less than the ambient atmospheric pressure. The principle of the discharge valve is explained here, because the sealing cover 121 of the subcooler is hermetically connected with the subcooler 113, the inner cavity forms a substantially closed space, the refrigerant contained therein will be evaporated and vaporized due to heat absorption by conduction at a normal external temperature, so that the pressure in the inner cavity is continuously increased, and when the predetermined pressure is reached, the pressure in the inner cavity needs to be relieved, so as to avoid the over-high pressure in the inner cavity. The predetermined pressure may be set differently according to the material and the structural form of the sealing cap 121 and the subcooler 113 of the subcooler and the external atmospheric pressure, for example, in a low altitude area, the predetermined pressure may be set to be slightly higher than the standard atmospheric pressure (101.3kPa), but if the freezing relay unit 1 is in an altitude area, the predetermined pressure may be set to be lower adaptively because the external atmospheric pressure is lower. In one example, the bleed valve may be, for example, a solenoid check bleed valve or a pressure-controlled check bleed valve. Alternatively, the discharge valve may be disposed at the bottom of the subcooler 113.

Optionally, the freezing transfer device 1 further includes a liquid replenishing interface, and can cooperate with an external automatic liquid replenishing device, and the external automatic liquid replenishing device can replenish the inner container 111 with the refrigerant liquid in real time, so as to ensure a safe freezing liquid level.

Referring to fig. 7 to 10 and 15 to 17, the negative pressure vaporizing device 2 includes: a negative pressure pump 21 and a second connection assembly 22 connected to the negative pressure pump 21; the negative pressure pump 21 pass through the second coupling assembling 22 with the inner chamber intercommunication, the negative pressure pump 21 is used for right negative pressure is taken out to the inner chamber. The negative pressure pump 21 can be a vacuum pump, and the second connecting component 22 is adapted to be connected to the first connecting component 13, so that the inner cavity is communicated with the negative pressure vaporizing device 2. In one embodiment, the second connecting component 22 is a blind-mate interface adapted to the first connecting component 13, and one of the second connecting component 22 and the first connecting component 13 is a male plug, and the other is a female plug. In another embodiment, the first connecting assembly 13 and the second connecting assembly 22 are the same, that is, only one connecting assembly of the refrigeration system connects the refrigeration relay device 1 and the negative pressure vaporizing device 2, respectively.

Preferably, the negative pressure vaporizing device 2 further comprises: an air-wet vaporizer 23 and/or a bacteriostatic filter 24; the air-wet vaporizer 23 and/or the bacteriostatic filter 24 are disposed between the negative pressure pump 21 and the second connection assembly 22.

In one example, the empty wet vaporizer 23 can prevent the low-temperature liquefied water from entering the negative pressure pump 21 when the negative pressure pump 21 pumps the negative pressure to the cavity filled with the refrigerant. It is generally difficult to completely prevent water vapor in the outside air from entering the cavity and the pipeline, and the arrangement of the air-wet vaporizer 23 can be used for removing condensed liquid water to avoid damaging the negative pressure pump 21. Preferably, the air-wet vaporizer 23 has a heat exchanger which can reduce the ambient temperature of the negative pressure pump 21, enhance the air flow, facilitate the heat dissipation of the negative pressure pump 21, reduce the loss, and improve the efficiency. The detailed structure and principle of the air wet vaporizer 23 and the heat exchanger can be understood by those skilled in the art according to the prior art, and will not be described herein.

The bacteriostatic filter 24 is arranged on the pipeline between the negative pressure pump 21 and the second connecting component 22, and is used for degerming and filtering. The arrangement of the bacteriostatic filter 24 ensures that bacteria in the pipeline cannot enter the inner cavity of the freezing transfer device 1, and ensures that the refrigerant is in a sterile environment.

Optionally, the negative pressure vaporization device 2 further includes: a fourth housing 25; the negative pressure pump 21 and the second connecting assembly 22 are accommodated in the fourth casing 25; the fourth housing 25 is adapted to be connected to the first housing 115 of the freezing and transfer device 1. In an example, the fourth housing 25 may be an ABS housing, for example, and has a recessed area adapted to the shape of the first housing 115, and the first housing 115 can be seated on the recessed area. The fourth housing 25 is used for accommodating the negative pressure pump 21, the second connection assembly 22, the air-wet vaporizer 23, the bacteriostatic filter 24, and the like. Preferably, the negative pressure vaporizing device 2 further includes soundproof cotton provided inside the fourth casing 25 for reducing the operation noise of the negative pressure vaporizing device 2.

Optionally, the refrigeration system further comprises an interaction device 3 and/or a parameter prompt device 4;

the interaction device 3 is arranged on the negative pressure vaporization device 2; the interaction device 3 is used for interactively inputting preset temperature parameters, and the negative pressure vaporization device 2 is used for pumping negative pressure to the inner cavity according to the preset temperature parameters so as to keep the temperature of the refrigerant contained in the inner cavity within a temperature range corresponding to the preset temperature parameters. It will be appreciated that based on the three-phase diagram of a particular refrigerant, a particular boiling point can be obtained for a particular pressure. Thus, the temperature of the refrigerant contained in the inner cavity of the freezing and transferring device 1 is controlled and adjusted by controlling the pressure value of the negative pressure pumped by the negative pressure pump 21. In an exemplary embodiment, the interactive device 3 includes a display screen and interactive keys, and may also include a touch display screen. The display screen can display the real-time running time of the negative pressure pump 21, the PID parameters of the negative pressure pump 21 and the like. The predetermined temperature parameter includes a target temperature of the refrigerant contained in the inner cavity of the freezing relay unit 1, an allowable temperature fluctuation, a PID parameter, or the like. For example, using subcooled liquid nitrogen as an example, the target temperature can be set between-196 ℃ and-210 ℃, and the allowable temperature fluctuation can be set as desired, for example, 1 ℃. In some embodiments, the temperature can be controlled and maintained according to the set PID parameters, and the temperature reduction and control can be realized through a program.

The parameter prompting device 4 is arranged on the freezing transfer device 1; the parameter prompting device 4 is used for acquiring and prompting at least one of positioning information of the freezing transfer device 1, a temperature of the refrigerant contained in the inner cavity, a pressure of the refrigerant, and a liquid level of the refrigerant. The positioning information may be, for example, GPS, BDS or GNSS positioning information. The parameter prompting device 4 may include a display 41 and a function switching button 42, for example, and by pressing the function switching button 42, information displayed on the display 41 can be switched.

Optionally, the refrigeration system further includes a control device 5, and the control device 5 may be integrated with the refrigeration relay device 1 or the negative pressure vaporization device 2, or may be independently disposed. The control device 5 is in communication connection with the negative pressure vaporization device 2 and the parameter prompt device 4 respectively, and may be in wired connection or in wireless connection, such as wifi or bluetooth connection. The control device 5 may include a PLC module, a positioning module, a transmission module, a sensor module, and the like, and the PLC module has a built-in PID calculation program that can adjust the rotation speed of the negative pressure pump 21, thereby accurately adjusting the temperature of the refrigerant contained in the inner cavity of the freezing transfer device 1. Optionally, the PLC module has a pressure relief program built therein, which can drive the vent valve on the container assembly 11 to vent and relieve pressure when the pressure in the internal cavity rises to a predetermined pressure. The positioning module is used for acquiring positioning information. The sensor module may include, for example, a thermocouple temperature sensor, a pressure sensor, a liquid level meter, and the like, which are respectively used to acquire the temperature of the refrigerant contained in the inner cavity, the pressure of the refrigerant, and the liquid level of the refrigerant. The transmission module can be used for being in communication connection with the negative pressure vaporization device 2 and the parameter prompting device 4. The transmission module may include, for example, a wireless module and/or a bluetooth module. Further, the transmission module may be used to communicate with a mobile terminal (e.g., a mobile phone), and in some embodiments, an operator may monitor at least one of the positioning information of the freezing and transferring device 1, the temperature of the refrigerant contained in the inner cavity, the pressure of the refrigerant, and the liquid level of the refrigerant through the mobile terminal, or may implement interaction with the negative pressure vaporizing device 2 through the mobile terminal to input the predetermined temperature parameter.

The following describes the use procedure of the freezing system provided in this embodiment, taking liquid nitrogen as the refrigerant as an example:

1. preparation process of supercooled liquid nitrogen:

the refrigeration transfer device 1 is connected according to the assembly position of the first shell 115 and the fourth shell 25 of the negative pressure vaporization device 2, the blind plugs of the first connecting assembly 13 and the second connecting assembly 22 assist in positioning the two, and the power supply of the negative pressure vaporization device 2 is connected.

The cover body assembly 12 of the freezing transfer device 1 is opened, liquid nitrogen is added into the inner container 111 according to the requirement, and the liquid nitrogen can be injected by matching with a matched automatic liquid supplementing device. After filling the liquid nitrogen, the cover assembly 12 is closed, and the first housing 115 and the second housing 122 are snapped to complete the sealing.

Inputting a preset temperature parameter on the interaction device 3, wherein the default value of the target temperature is-210 ℃, clicking a start confirmation key, operating the negative pressure pump 21 and the air-wet vaporizer 23, monitoring the temperature, the pressure or the liquid level of the liquid nitrogen in the inner container 111 in real time through a display screen of the interaction device 3 and the parameter prompt device 4, and starting the automatic liquid supplementing device to supplement the liquid in the inner container 111 when the liquid level is lower than the preset lowest liquid level value so as to ensure the liquid level of the liquid nitrogen. When the temperature of the supercooled liquid nitrogen reaches the target temperature, the rotating speed of the negative pressure pump 21 is adjusted through a PID calculation program built in the PLC module to keep the temperature constant, and the preparation of the supercooled liquid nitrogen is completed.

2. Freezing process of biological tissue:

after the preparation of the supercooled liquid nitrogen is completed, the cover body assembly 12 of the freezing transfer device 1 is opened, a single freezing storage tube can be placed, a storage box comprising a plurality of freezing storage tubes can also be placed for freezing, the cover body assembly 12 is closed, the buckles of the first shell 115 and the second shell 122 are buckled, and sealing is completed.

Optionally, sign indicating number discernment is swept in freezing transfer device 1 collocation vision, can cooperate the two-dimensional code information storage of cryopreserving pipe, conveniently seeks and control. In the process, the temperature, the pressure or the liquid level of the liquid nitrogen in the inner container 111 can be monitored in real time through the parameter prompt device 4 or the mobile terminal. It is understood that the freezing process of the biological tissue may be performed after the freezing relay device 1 is separated from the negative pressure vaporizing device 2, or may be performed when the freezing relay device 1 is connected to the negative pressure vaporizing device 2, but before the cover assembly 12 is opened, the negative pressure pump 21 of the negative pressure vaporizing device 2 should be stopped to make the pressure in the inner cavity rise to be approximately equal to the external atmospheric pressure.

3. The transfer process comprises:

in the process, the freezing transfer device 1 is separated from the negative pressure vaporization device 2, and after the freezing storage of the biological tissues is completed, the freezing transfer device 1 can be carried by matching with a matched AGV composite robot, and the operation can be carried by an operator according to the requirement. Positioning information of the freezing transfer device 1, the temperature, the pressure and the liquid level of liquid nitrogen can be monitored in real time through the mobile terminal in the transfer process, and the transfer safety of biological tissues is guaranteed. If the pressure in the interior chamber rises to a predetermined pressure during transit, the vent valve on the container assembly 11 vents to relieve pressure to ensure safe storage.

In summary, the present invention provides a refrigeration system comprising: a refrigeration transfer device and a negative pressure vaporization device; the freezing transfer device is detachably connected with the negative pressure vaporization device; the freezing transfer device is provided with an inner cavity, and the inner cavity is used for accommodating a refrigerant; when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporization device is used for pumping negative pressure to the inner cavity so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling temperature of the refrigerant under the external atmospheric pressure.

So the configuration, through negative pressure vaporization device to freezing transfer device's inner chamber take out the negative pressure, reduced the boiling point of refrigerant to make the temperature of the refrigerant of holding in freezing transfer device's the inner chamber reduce, improved the cooling rate to biological tissue. Therefore, on one hand, the vitrification of the cryoprotectant is facilitated, so that the concentration of the cryoprotectant in the frozen liquid can be reduced, the refrigeration toxicity and damage are reduced, and the quality of the refrigerated biological tissues is improved. On the other hand, the limitation on the size of the stored biological tissue is reduced, and a larger size of the biological tissue can be stored, which leads to a wider storage range. On the other hand, the freezing transfer device and the negative pressure vaporization device are separable, when the freezing transfer device and the negative pressure vaporization device are combined, the negative pressure vaporization device can pump negative pressure to the inner cavity of the freezing transfer device according to required pressure, so that the preservation temperature can be accurately controlled, and the whole freezing system can be used as a long-term storage system for vitrification freezing preservation; when the refrigeration transfer device is separated from the negative pressure vaporization device, the low-temperature refrigerant contained in the refrigeration transfer device can keep low temperature for a certain time, and can be used for transferring and transferring biological tissues.

It should be noted that, several of the above embodiments may be combined with each other. The above description is only for the purpose of describing the preferred embodiments of the present invention, and is not intended to limit the scope of the present invention, and any variations and modifications made by those skilled in the art based on the above disclosure are within the scope of the appended claims.

Claims (12)

1. A refrigeration system, comprising: a refrigeration transfer device and a negative pressure vaporization device; the refrigeration transfer device is detachably connected with the negative pressure vaporization device; the freezing transfer device is provided with an inner cavity, and the inner cavity is used for accommodating a refrigerant;

when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporization device is used for pumping negative pressure to the inner cavity.

2. The refrigeration system of claim 1, wherein the refrigeration transfer device comprises a container assembly and a cover assembly, the cover assembly being openably and closably connected to the container assembly; the inner cavity is closed when the cover assembly is connected with the container assembly.

3. The freezing system of claim 2, wherein the freezing relay device further comprises a first connecting assembly, the first connecting assembly is connected to the container assembly and is in communication with the inner cavity, and a movable end of the first connecting assembly is configured to be connected to the negative pressure vaporization device.

4. The refrigeration system of claim 3 wherein the first connection assembly has a shut-off valve;

when the refrigeration transfer device is connected with the negative pressure vaporization device, the stop valve is communicated;

and when the refrigeration transfer device is separated from the negative pressure vaporization device, the stop valve is closed.

5. The freezing system of claim 2, wherein the freezing relay further comprises a drain valve coupled to the container assembly and in communication with the interior chamber;

when the refrigeration transfer device is separated from the negative pressure vaporization device and the pressure in the inner cavity does not exceed a preset pressure, the discharge valve is closed;

when the refrigeration transfer device is separated from the negative pressure vaporization device and the pressure in the inner cavity exceeds the preset pressure, the discharge valve is conducted, the pressure in the inner cavity is relieved, and the discharge valve is closed until the pressure in the inner cavity does not exceed the preset pressure; wherein the predetermined pressure is not less than the ambient atmospheric pressure.

6. The refrigeration system of claim 2 wherein the container assembly comprises: the inner container, the first heat-preserving sleeve and the subcooler; the cover assembly includes: sealing the subcooler;

the first heat-preserving sleeve is sleeved outside the inner container; the first heat-preserving sleeve and the inner container are contained in the subcooler together; the subcooler sealing cover can be connected with the subcooler in a sealing way in an opening and closing way.

7. The refrigeration system of claim 6 wherein the container assembly further comprises a shock pad positioned at a bottom outside the inner container and abutting the inner container and the subcooler, respectively.

8. The refrigeration system of claim 6 wherein the container assembly further comprises: the first shell and the second heat-insulating sleeve; the cover assembly further includes: a second shell and a third insulating sleeve; the first shell is used for being connected with the second shell in a matching manner;

the second heat-insulating sleeve is sleeved outside the subcooler; the second heat-preserving sleeve and the subcooler are contained in the first shell together; the subcooler sealing cover is connected with the second shell through the third heat-preserving sleeve.

9. The refrigeration system of claim 1 wherein the negative pressure vaporization device comprises: the negative pressure pump and a second connecting component connected with the negative pressure pump; the negative pressure pump passes through the second coupling assembling with the inner chamber intercommunication is used for right negative pressure is taken out to the inner chamber.

10. The refrigeration system of claim 9 wherein the negative pressure vaporization device further comprises: an air-wet vaporizer and/or bacteriostatic filter; the air wet vaporizer and/or the bacteriostatic filter are/is arranged between the negative pressure pump and the second connecting component.

11. The refrigeration system of claim 9 wherein the negative pressure vaporization device further comprises: a fourth housing; the negative pressure pump and the second connecting assembly are contained in the fourth shell; the fourth shell is connected with the first shell of the freezing transfer device in a matching mode.

12. The refrigeration system of claim 1, further comprising: interaction means and/or parameter prompt means;

the interaction device is arranged on the negative pressure vaporization device; the interaction device is used for interactively inputting preset temperature parameters, and the negative pressure vaporization device is used for pumping negative pressure to the inner cavity according to the preset temperature parameters so as to keep the temperature of the refrigerant contained in the inner cavity within a temperature interval corresponding to the preset temperature parameters;

the parameter prompting device is arranged on the freezing transfer device; the parameter prompting device is used for acquiring and prompting at least one of positioning information of the freezing transfer device, the temperature of the refrigerant contained in the inner cavity, the pressure of the refrigerant and the liquid level of the refrigerant.

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