CN116548425A - Refrigeration system - Google Patents

Abstract

The invention provides a refrigerating system, which comprises a refrigerating transfer device and a negative pressure vaporizing device; the refrigeration transfer device is detachably connected with the negative pressure vaporization device; the refrigeration transfer device is provided with an inner cavity for accommodating a refrigerant; when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporizing device is used for pumping negative pressure to the inner cavity so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling point temperature of the refrigerant under the external atmospheric pressure. Therefore, the negative pressure is pumped into the inner cavity of the refrigeration transfer device through the negative pressure vaporization device, so that the boiling point of the refrigerant is reduced, the temperature of the refrigerant accommodated in the inner cavity of the refrigeration transfer device is reduced, and the cooling rate of biological tissues is improved. Is favorable for vitrification of the cryoprotectant, thereby reducing the concentration of the cryoprotectant, relieving the refrigeration toxicity and damage and improving the quality of the refrigerated biological tissue.

Description

Refrigeration system

Technical Field

The invention relates to the technical field of medical appliances, in particular to a refrigerating system.

Background

In the field of human assisted reproduction, low-temperature cryopreservation of embryos and ova is an important component, and vitrification freezing is a commonly used embryo cryopreservation technology at present. The embryo cryopreservation technology utilizes high-concentration cell protection liquid to treat cells and tissues so as to improve the glass transition temperature, and improves the cooling rate so as to realize more efficient vitrification. In the specific method for realizing vitrification, the Cryotop method is widely applied in terms of simple operation, higher obtained freezing rate, and higher survival rate and development rate of cells after vitrification preservation.

The Cryotop method was a high-speed freezing method proposed by Kuwayama according to the principle of minimizing the volume of solution in 2005. The carrier of this embodiment is made by attaching a thin plastic strip to a plastic handle. The operation is completed under a stereoscopic microscope, firstly, the oocyte is loaded on a plastic carrier by a glass capillary tube with the inner diameter slightly larger than the cell diameter, then, the capillary tube is used, and the redundant cryoprotectant liquid around the oocyte is sucked away by utilizing the capillary tube principle, so that the oocyte is only covered by a very thin liquid film, and then, the plastic carrier carrying the oocyte is inserted into liquid nitrogen and stored in the liquid nitrogen for a long time. The cooling rate of the method can reach 12,000+/-1,500K/min. However, this method has problems such as insufficient cooling rate of cells, thus requiring the use of high concentration of cryoprotectant, high cytotoxicity, and inability to preserve larger diameter cells.

Disclosure of Invention

The invention aims to provide a refrigeration system which solves a series of problems caused by insufficient cooling rate of the existing frozen and preserved biological tissues.

In order to solve the above technical problems, the present invention provides a refrigeration system, which includes: a refrigeration transfer device and a negative pressure vaporization device; the refrigeration transfer device is detachably connected with the negative pressure vaporization device; the refrigeration transfer device is provided with an inner cavity for accommodating a refrigerant;

when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporization device is used for pumping negative pressure to the inner cavity.

Optionally, in the refrigeration system, the refrigeration transfer device includes a container assembly and a cover assembly, and the cover assembly is connected with the container assembly in an openable and closable manner; when the cover assembly is connected with the container assembly, the inner cavity is formed in a sealing mode.

Optionally, in the refrigeration system, the refrigeration transfer device further includes a first connection component, the first connection component is connected with the container component and is communicated with the inner cavity, and a movable end of the first connection component is used for being connected with the negative pressure vaporization device;

optionally, in the refrigeration system, the first connection assembly has a shut-off valve;

when the refrigeration transfer device is connected with the negative pressure vaporization device, the stop valve is connected;

and when the refrigeration transfer device is separated from the negative pressure vaporization device, the stop valve is closed.

Optionally, in the refrigeration system, the refrigeration relay apparatus further includes a discharge valve connected to the container assembly and in communication with the interior cavity;

when the refrigeration transfer device is separated from the negative pressure vaporization device and the pressure in the inner cavity does not exceed the preset pressure, the discharge valve is closed;

when the refrigeration transfer device is separated from the negative pressure vaporization device and the pressure in the inner cavity exceeds the preset pressure, the discharge valve is conducted, the inner cavity is depressurized, and the discharge valve is closed until the pressure in the inner cavity does not exceed the preset pressure; wherein the predetermined pressure is not less than the external atmospheric pressure.

Optionally, in the refrigeration system, the container assembly includes: the refrigerator comprises an inner container, a first heat preservation sleeve and a supercooler; the cover assembly includes: a subcooler seal cover;

the first heat preservation sleeve is sleeved outside the inner container; the first heat preservation sleeve and the liner are contained in the subcooler together; the subcooler sealing cover is connected with the subcooler in a sealing way in an openable and closable manner.

Optionally, in the refrigeration system, the container assembly further includes a shock pad, and the shock pad is located at the bottom outside the liner and abuts against the liner and the subcooler respectively.

Optionally, in the refrigeration system, the container assembly further comprises: the first shell and the second heat preservation sleeve; the cover assembly further includes: the second shell and the third heat preservation sleeve; the first shell is used for being connected with the second shell in an adaptive manner;

the second heat preservation sleeve is sleeved outside the subcooler; the second heat preservation sleeve and the subcooler are contained in the first shell together; the subcooler sealing cover is connected with the second shell through the third heat preservation sleeve.

Optionally, in the refrigeration system, the negative pressure vaporizing device includes: a negative pressure pump and a second connecting component connected with the negative pressure pump; the negative pressure pump is communicated with the inner cavity through the second connecting component and is used for pumping negative pressure to the inner cavity.

Optionally, in the refrigeration system, the negative pressure vaporizing device further includes: an empty wet vaporizer and/or a bacteriostatic filter; the air wet vaporizer and/or the antibacterial filter are arranged between the negative pressure pump and the second connecting component.

Optionally, in the refrigeration system, the negative pressure vaporizing device further includes: a fourth housing; the negative pressure pump and the second connecting component are accommodated in the fourth shell; the fourth shell is connected with the first shell of the freezing transfer device in an adaptive mode.

Optionally, in the refrigeration system, the refrigeration system further includes: interaction means and/or parameter prompting means;

the interaction device is arranged on the negative pressure vaporization device; the interaction device is used for interactively inputting preset temperature parameters, and the negative pressure vaporization device pumps negative pressure to the inner cavity according to the preset temperature parameters so as to keep the temperature of the refrigerant accommodated in the inner cavity within a temperature interval corresponding to the preset temperature parameters;

the parameter prompting device is arranged on the refrigeration transfer device; the parameter prompting device is used for acquiring and prompting at least one of positioning information of the freezing transfer device, the temperature of the refrigerant contained in the inner cavity, the pressure of the refrigerant and the liquid level of the refrigerant.

In summary, the refrigeration system provided by the present invention includes: a refrigeration transfer device and a negative pressure vaporization device; the refrigeration transfer device is detachably connected with the negative pressure vaporization device; the refrigeration transfer device is provided with an inner cavity for accommodating a refrigerant; when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporizing device is used for pumping negative pressure to the inner cavity so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling point temperature of the refrigerant under the external atmospheric pressure.

So configured, the negative pressure is pumped to the inner cavity of the refrigeration transfer device through the negative pressure vaporization device, so that the boiling point of the refrigerant is reduced, the temperature of the refrigerant accommodated in the inner cavity of the refrigeration transfer device is reduced, and the cooling rate of biological tissues is improved. Therefore, on one hand, the vitrification of the cryoprotectant is facilitated, so that the concentration of the cryoprotectant can be reduced, the refrigeration toxicity and damage are reduced, and the quality of the refrigerated biological tissue is improved. On the other hand, the limitation of the size of the preserved biological tissue is reduced, the biological tissue with larger size can be preserved, and the preservation range is wider. In the other aspect, the freezing transfer device and the negative pressure vaporization device are separable, and when the freezing transfer device and the negative pressure vaporization device are combined, the negative pressure vaporization device can pump negative pressure to the inner cavity of the freezing transfer device according to the required pressure, so that the accurate control of the preservation temperature is realized, and the whole freezing system can be used as a long-term storage system for vitrification freezing storage; when the freezing transfer device is separated from the negative pressure vaporization device, the low-temperature refrigerant contained in the freezing transfer device can keep low temperature for a certain time, and can be transferred by biological tissues.

Drawings

Those of ordinary skill in the art will appreciate that the figures are provided for a better understanding of the present invention and do not constitute any limitation on the scope of the present invention. Wherein:

FIG. 1 is a front view of a refrigeration system according to an embodiment of the present invention;

FIG. 2 is a side view of a refrigeration system according to an embodiment of the present invention;

FIG. 3 is a top view of a refrigeration system according to an embodiment of the present invention;

FIG. 4 is a front view of a freeze transfer device according to an embodiment of the present invention;

FIG. 5 is a side view of a freeze transfer device according to an embodiment of the present invention;

FIG. 6 is a top view of a freeze transfer device according to an embodiment of the present invention;

FIG. 7 is a front view of a negative pressure vaporizing device of an embodiment of the present invention;

FIG. 8 is a side view of a negative pressure vaporizing device of an embodiment of the present invention;

FIG. 9 is a top view of a negative pressure vaporizing device according to an embodiment of the present invention;

FIG. 10 is a rear view of a negative pressure vaporizing device of an embodiment of the present invention;

FIG. 11 is an exploded view of a front view of a refrigeration system according to an embodiment of the present invention;

FIG. 12 is an exploded side view of a refrigeration system of an embodiment of the present invention;

FIG. 13 is a schematic view of a container assembly and a lid assembly according to an embodiment of the present invention;

FIG. 14 is an exploded view of a container assembly and a cap assembly according to an embodiment of the present invention;

FIG. 15 is an exploded view of the negative pressure vaporizing device of the present embodiment in the front view;

FIG. 16 is an exploded side view of a negative pressure vaporizing device according to an embodiment of the present invention;

fig. 17 is a partially exploded view of a negative pressure vaporizing device according to an embodiment of the present invention.

In the accompanying drawings:

1-a refrigeration transfer device; 11-a container assembly; 111-an inner container; 112-a first thermal sleeve; 113-subcooler; 114-a shock pad; 115-a first housing; 1151-a handle; 12-a cover assembly; 121-a subcooler seal cap; 1211-a seal ring; 122-a second housing; 1221-a handle; 13-a first connection assembly;

2-a negative pressure vaporization device; 21-a negative pressure pump; 22-a second connection assembly; 23-empty wet vaporizer; 24-a bacteriostatic filter; 25-fourth housing;

3-interaction means; 4-parameter prompting means; 41-a display screen; 42-a function switching button; 5-control means.

Detailed Description

The invention will be described in further detail with reference to the drawings and the specific embodiments thereof in order to make the objects, advantages and features of the invention more apparent. It should be noted that the drawings are in a very simplified form and are not drawn to scale, merely for convenience and clarity in aiding in the description of embodiments of the invention. Furthermore, the structures shown in the drawings are often part of actual structures. In particular, the drawings are shown with different emphasis instead being placed upon illustrating the various embodiments.

As used in this disclosure, the singular forms "a," "an," and "the" include plural referents, the term "or" are generally used in the sense of comprising "and/or" and the term "several" are generally used in the sense of comprising "at least one," the term "at least two" are generally used in the sense of comprising "two or more," and the term "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying any relative importance or number of features indicated. Thus, a feature defining "first," "second," "third," or the like, may explicitly or implicitly include one or at least two such features, with "one end" and "another end" and "proximal end" and "distal end" generally referring to the corresponding two portions, including not only the endpoints. Furthermore, as used in this disclosure, "mounted," "connected," and "disposed" with respect to another element should be construed broadly to mean generally only that there is a connection, coupling, mating or transmitting relationship between the two elements, and that there may be a direct connection, coupling, mating or transmitting relationship between the two elements or indirectly through intervening elements, and that no spatial relationship between the two elements is to be understood or implied, i.e., that an element may be in any orientation, such as internal, external, above, below, or to one side, of the other element unless the context clearly dictates otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances. Furthermore, directional terms, such as above, below, upper, lower, upward, downward, left, right, etc., are used with respect to the exemplary embodiments as they are shown in the drawings, upward or upward toward the top of the corresponding drawing, downward or downward toward the bottom of the corresponding drawing.

The invention aims to provide a refrigeration system which solves a series of problems caused by insufficient cooling rate of the existing frozen and preserved biological tissues.

The following description refers to the accompanying drawings.

The inventors found that, for the cryopreservation of biological tissues (such as embryos or cells) using a specific refrigerant, the specific refrigerant has a specific temperature under normal conditions, for example, liquid nitrogen, in an atmospheric pressure environment (refer to the vicinity of one standard atmospheric pressure), since the temperature of the normal temperature environment is higher than the boiling point of liquid nitrogen, liquid nitrogen is unlikely to be completely in an ideal adiabatic environment and absorbs heat from the external environment, resulting in that it is at a boiling point temperature in an equilibrium state, with a small amount of liquid nitrogen being vaporized continuously, while the liquid nitrogen is maintained at an atmospheric pressure boiling point temperature of approximately-196 ℃. At which time it is known about the rate of cooling of biological tissue of a particular size. If different refrigerants are replaced to increase the cooling rate, the use cost is greatly increased by adopting a refrigerant with a lower temperature, such as liquid helium.

The inventors have further found that liquid nitrogen can remain in a liquid state as low as-210 c according to the three-phase diagram of nitrogen, and liquid nitrogen below the boiling point temperature of liquid nitrogen is referred to as supercooled liquid nitrogen if the temperature of liquid nitrogen is lowered. It can be appreciated that the use of supercooled liquid nitrogen can increase the rate of cooling of biological tissue. Therefore, on one hand, the vitrification of the cryoprotectant is facilitated, so that the concentration of the cryoprotectant can be reduced, the refrigeration toxicity and damage are reduced, and the quality of the refrigerated biological tissue is improved. On the other hand, the limitation of the size of the preserved biological tissue is reduced, the biological tissue with larger size can be preserved, and the preservation range is wider. Of course, expansion to other refrigerants, such as liquid carbon dioxide or liquid helium, both subcooled liquid carbon dioxide or subcooled liquid helium can achieve lower temperatures than the original boiling temperature liquid carbon dioxide or liquid helium, effectively increasing the refrigeration efficiency under this type of refrigerant. In the industrialized age, it is not a difficult matter to produce a subcooled refrigerant at a lower temperature than the original boiling temperature liquid refrigerant. However, in some small-scale or portable applications, the use of subcooled refrigerants is limited because the bulky refrigeration equipment is often stationary and inconvenient to handle. In particular, in some cases where it is necessary to freeze and transport biological tissues, supercooled refrigerants produced in refrigeration equipment such as factories often absorb heat and heat up to the boiling point when they are transported to the freeze relay device, and this is difficult to use.

Referring to fig. 1 to 3, based on the above study, an embodiment of the present invention provides a refrigeration system, which includes: a refrigeration transfer device 1 and a negative pressure vaporization device 2; the refrigeration transfer device 1 is detachably connected with the negative pressure vaporization device 2; the refrigeration transfer device 1 is provided with an inner cavity for accommodating a refrigerant; when the refrigeration transfer device 1 is connected with the negative pressure vaporization device 2, the inner cavity is communicated with the negative pressure vaporization device 2; the negative pressure vaporizing device 2 is used for pumping negative pressure to the inner cavity so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling point temperature of the refrigerant under the external atmospheric pressure.

The boiling point temperature of a particular refrigerant may be reduced by applying a negative pressure to the refrigerant. Thus, if the refrigerant contained in the inner chamber of the refrigeration relay apparatus 1 is negatively pressurized, the refrigerant contained in the inner chamber is brought to a temperature lower than the boiling point temperature thereof at the outside atmospheric pressure, and an supercooled refrigerant can be produced. Therefore, although a part of refrigerant is lost, a large-volume refrigeration device is not arranged, and only the negative pressure vaporization device 2 with a small volume is used, so that the volume of the whole refrigeration system is effectively reduced, and the refrigeration system is convenient to carry and move. When the refrigeration relay apparatus 1 is connected to the negative pressure vaporizing apparatus 2, the negative pressure vaporizing apparatus 2 can maintain the refrigerant in the refrigeration relay apparatus 1 in a supercooled state by drawing a negative pressure into the inner chamber of the refrigeration relay apparatus 1. Further, the freezing relay device 1 and the negative pressure vaporization device 2 can be separated, after the freezing relay device 1 and the negative pressure vaporization device 2 are separated, the inner cavity of the freezing relay device 1 can be restored to the external atmospheric pressure, and meanwhile, the supercooled refrigerant accommodated therein can also be maintained in a supercooled state for a period of time, so that the possibility is created for transferring the freezing relay device 1. It will be appreciated that, since the negative pressure vaporizing device 2 pumps negative pressure into the inner cavity of the refrigeration relay device 1, gaseous refrigerant will be pumped, and generally gaseous refrigerant will be directly discharged to the outside, so the refrigerant should be selected from the environment-friendly types, and the refrigerant includes but is not limited to nitrogen, carbon dioxide, helium, etc.

An exemplary embodiment of a refrigeration system is described below with reference to fig. 1-17. It should be understood that the illustration of fig. 1-17 is merely one example of a refrigeration system and is not intended to be limiting.

Referring to fig. 4 to 6 and 11 to 14, the refrigeration relay apparatus 1 includes: a container assembly 11 and a cover assembly 12, wherein the cover assembly 12 is connected with the container assembly 11 in an openable and closable manner; when the cap assembly 12 is connected to the container assembly 11, the interior cavity is closed.

Optionally, the container assembly 11 includes: an inner container 111, a first heat-retaining jacket 112, and a supercooler 113; the cover assembly 12 includes: a subcooler seal cover 121; the inner container 111 is used for accommodating the refrigerant; the first heat-preserving sleeve 112 is sleeved outside the inner container 111; the first heat-preserving cover 112 and the inner container 111 are accommodated in the subcooler 113 together; the subcooler sealing cover 121 is openably and closably connected to the subcooler 113 in a sealing manner.

In an alternative example, the inner container 111 is a double-layer vacuum stainless steel barrel, the upper end of the inner container 111 is opened, the inner container is used for containing the refrigerant, and the inner surface and/or the outer surface of the inner container 111 are/is provided with a silver coating to reduce radiation heat dissipation. The first heat-preserving jacket 112 is made of ethylene-vinyl acetate copolymer foam (EVA), and is sleeved outside the inner container 111 to reduce heat exchange between the refrigerant in the inner container 111 and the outside. The supercooler 113 is a tub made of Polyoxymethylene (POM) material, the upper end of the supercooler 113 is opened, and the inner container 111 surrounding the first insulation cover 112 may be loaded into the supercooler 113 from the open end of the supercooler 113. The subcooler sealing cover 121 can seal the open end of the subcooler 113 such that the interior of the subcooler 113 forms a relatively airtight cavity. Thereby, the negative pressure can be pumped into the inner cavity through the negative pressure vaporizing device 2.

Optionally, the subcooler sealing cover 121 has a sealing ring 1211, where the sealing ring 1211 may be a silica gel sealing ring, and is adapted to the shape of the open end of the subcooler 113, and the subcooler sealing cover 121 can be connected with the open end of the subcooler 113 in a sealing manner through the sealing ring 1211. Preferably, the container assembly 11 further includes a shock pad 114, where the shock pad 114 is accommodated in the subcooler 113 and is located at the bottom outside the liner 111, and the shock pad 114 is respectively abutted against and connected with the liner 111 and the subcooler 113, so that the shock pad can absorb the shock of the refrigeration transfer device 1 in the transferring process, and reduce the shock of the subcooler 113, thereby reducing the loss of the refrigerant. The material of the shock pad 114 may be, for example, foamed rubber.

Further, the container assembly 11 further includes: a first housing 115 and a second insulating jacket (not shown) located within the first housing 115; the cover assembly 12 further includes: a second housing 122 and a third insulating sleeve (not shown) located within the second housing 122; the first housing 115 is connected to the second housing 122 in a fitting manner; the second heat preservation sleeve is sleeved outside the subcooler 113; the second insulation jacket and the subcooler 113 are accommodated in the first housing 115 together; the subcooler sealing cover 121 is connected with the second casing 122 through the third insulation sleeve. Preferably, the first housing 115 and the second housing 122 are connected by a snap fit.

In an alternative example, the first housing 115 and the second housing 122 are housings made of acrylonitrile-butadiene-styrene (ABS) material, the second insulation jacket and the third insulation jacket are made of EVA, and the second insulation jacket is sleeved outside the subcooler 113 for reducing heat exchange between the inside and the outside of the subcooler 113. The third insulation cover serves to reduce heat exchange of the subcooler 113 with the outside through the subcooler sealing cover 121. The first housing 115 and the second housing 122 of the ABS have better mechanical properties, are impact-resistant, and are suitable for transferring and transporting. Optionally, the first housing 115 has a handle 1151, the handle 1151 of the first housing 115 facilitates handling, and the second housing 122 has a handle 1221, the handle 1221 facilitating opening of the subcooler sealing cover 121.

Optionally, the refrigeration relay apparatus 1 further includes a first connection assembly 13, where the first connection assembly 13 is connected to the container assembly 11, for example, the first connection assembly 13 is disposed at the bottom of the container assembly 11 and is in communication with the inner cavity, and a movable end of the first connection assembly 13 is used to communicate with the negative pressure vaporization apparatus 2. Further, the first connection assembly 13 has a stop valve; when the refrigeration transfer device 1 is connected with the negative pressure vaporization device 2, the stop valve is conducted so that the inner cavity is communicated with the negative pressure vaporization device 2 through the first connecting component 13; when the refrigeration relay apparatus 1 is separated from the negative pressure vaporizing apparatus 2, the shut-off valve is closed. The first connection assembly 13 mainly serves as a connection port with the negative pressure vaporizing device 2. In one exemplary embodiment, the first connection element 13 comprises a blind plug-in connection, for example, which can be quickly plugged into a corresponding second connection element 22 of the negative pressure vaporization apparatus 2. In one example, the shut-off valve may be, for example, a solenoid valve or a manually operated valve.

Optionally, the refrigeration relay apparatus 1 further includes a discharge valve connected to the container assembly 11 and communicating with the inner cavity; when the refrigeration transfer device 1 is separated from the negative pressure vaporization device 2 and the pressure in the inner cavity does not exceed the preset pressure, the discharge valve is closed; when the refrigeration transfer device 1 is separated from the negative pressure vaporization device 2 and the pressure in the inner cavity exceeds the preset pressure, the discharge valve is conducted, the inner cavity is depressurized, and the discharge valve is closed until the pressure in the inner cavity does not exceed the preset pressure; wherein the predetermined pressure is not less than the external atmospheric pressure. The arrangement principle of the discharge valve is described herein, and since the subcooler sealing cover 121 is in sealing connection with the subcooler 113, the inner cavity forms a substantially closed space, and the refrigerant contained in the inner cavity is vaporized and evaporated due to heat absorption by conduction at ordinary external temperature, so that the pressure in the inner cavity is continuously increased, and the inner cavity needs to be relieved when reaching the predetermined pressure, so as to avoid the excessive pressure in the inner cavity. The predetermined pressure may be set differently depending on the material and structural form of the subcooler sealing cover 121 and the subcooler 113 and the external atmospheric pressure, for example, in a low altitude area, the predetermined pressure may be set to be slightly higher than the standard atmospheric pressure (101.3 kPa), but if the refrigeration relay apparatus 1 is in a plateau area, the predetermined pressure may be set to be low adaptively due to the low external atmospheric pressure. In one example, the vent valve may be, for example, a solenoid one-way vent valve or a pressure-controlled one-way vent valve. Alternatively, the discharge valve may be provided at the bottom of the subcooler 113.

Optionally, the refrigeration transfer device 1 further includes a fluid infusion interface, which can be matched with an external automatic fluid infusion device, so as to perform real-time fluid infusion on the inner container 111 to ensure a safe refrigeration liquid level.

Referring to fig. 7 to 10 and 15 to 17, the negative pressure vaporizing device 2 includes: a negative pressure pump 21 and a second connection assembly 22 connected to the negative pressure pump 21; the negative pressure pump 21 is communicated with the inner cavity through the second connecting component 22, and the negative pressure pump 21 is used for pumping negative pressure to the inner cavity. The negative pressure pump 21 may be, for example, a vacuum pump, and the second connection assembly 22 is adapted to be connected to the first connection assembly 13, so as to communicate the inner cavity with the negative pressure vaporizing device 2. In one embodiment, the second connecting component 22 is a blind mating interface adapted to the first connecting component 13, one of the second connecting component 22 and the first connecting component 13 is a male mating, and the other is a female mating. In another embodiment, the first connecting component 13 and the second connecting component 22 are the same, that is, only one connecting component of the refrigeration system is connected to the refrigeration relay 1 and the negative pressure vaporizing device 2 respectively.

Preferably, the negative pressure vaporizing device 2 further comprises: an empty wet vaporizer 23 and/or a bacteriostatic filter 24; the air wet vaporizer 23 and/or the bacteriostatic filter 24 are arranged between the negative pressure pump 21 and the second connection assembly 22.

In one example, the air-wet vaporizer 23 can prevent the cryogenic liquefied water from entering the negative pressure pump 21 when the negative pressure pump 21 applies negative pressure to the inner chamber containing the refrigerant. It is generally difficult to completely stop water vapor in the outside air from entering the inner cavity and the pipeline, and the arrangement of the air-wet vaporizer 23 can be used for removing condensed liquid water, so as to avoid damaging the negative pressure pump 21. Preferably, the air-wet vaporizer 23 has a heat exchanger, which can reduce the ambient temperature of the negative pressure pump 21, enhance the air flow, facilitate the heat dissipation of the negative pressure pump 21, reduce the loss, and improve the efficiency. The specific structure and principle of the air wet vaporizer 23 and the heat exchanger will be understood by those skilled in the art from the prior art, and will not be explained here.

A bacteriostatic filter 24 is provided on the line between the negative pressure pump 21 and the second connection assembly 22 for the sterile filtration. The arrangement of the bacteriostatic filter 24 ensures that bacteria in the pipeline cannot enter the inner cavity of the refrigeration transfer device 1, and ensures that the refrigerant is in a sterile environment.

Optionally, the negative pressure vaporizing device 2 further includes: a fourth housing 25; the negative pressure pump 21 and the second connecting assembly 22 are accommodated in the fourth housing 25; the fourth housing 25 is adapted to be connected to the first housing 115 of the refrigeration relay 1. In one example, the fourth housing 25 may be an ABS housing, for example, having a recess adapted to the shape of the first housing 115, on which recess the first housing 115 can rest. The fourth housing 25 is used for accommodating the negative pressure pump 21, the second connecting assembly 22, the air-wet vaporizer 23, the bacteriostatic filter 24 and the like. Preferably, the negative pressure vaporizing device 2 further includes soundproof cotton provided inside the fourth housing 25 for reducing the operation noise of the negative pressure vaporizing device 2.

Optionally, the refrigeration system further comprises an interaction device 3 and/or a parameter prompting device 4;

the interaction device 3 is arranged on the negative pressure vaporization device 2; the interaction device 3 is configured to interactively input a predetermined temperature parameter, and the negative pressure vaporization device 2 pumps negative pressure to the inner cavity according to the predetermined temperature parameter, so that the temperature of the refrigerant contained in the inner cavity is kept within a temperature interval corresponding to the predetermined temperature parameter. It will be appreciated that a particular boiling point may be obtained when the pressure is at a particular value based on the three-phase diagram of a particular refrigerant. Thereby controlling and adjusting the temperature of the refrigerant contained in the inner chamber of the refrigeration relay apparatus 1 by controlling the value of the negative pressure pump 21. In one example, the interaction device 3 includes a display screen and interaction keys, and may also include a touch-type display screen. The display screen may display real-time running time of the negative pressure pump 21, PID parameters of the negative pressure pump 21, and the like. The predetermined temperature parameter includes a target temperature of the refrigerant contained in the inner chamber of the refrigeration relay apparatus 1, an allowable temperature fluctuation, a PID parameter, or the like. For example, in the case of supercooled liquid nitrogen, the target temperature can be set between-196℃and-210℃and the allowable temperature fluctuation can be set as desired, for example, 1 ℃. In some embodiments, the temperature regulation and maintenance can be controlled according to the set PID parameters, and the temperature reduction and control can be realized through a program.

The parameter prompting device 4 is arranged on the refrigeration transit device 1; the parameter prompting device 4 is configured to obtain and prompt at least one of positioning information of the refrigeration relay device 1, a temperature of the refrigerant contained in the inner cavity, a pressure of the refrigerant, and a liquid level of the refrigerant. The positioning information may be, for example, GPS, BDS or GNSS positioning information. The parameter presentation device 4 may include a display 41 and a function switching button 42, for example, and can switch information displayed on the display 41 by pressing the function switching button 42.

Optionally, the refrigeration system further comprises a control device 5, and the control device 5 can be integrated on the refrigeration transfer device 1 or the negative pressure vaporization device 2 or can be independently arranged. The control device 5 is respectively in communication connection with the negative pressure vaporizing device 2 and the parameter prompting device 4, and can be in wired connection or wireless connection, such as wifi or Bluetooth connection. The control device 5 may include a PLC module, a positioning module, a transmission module, a sensor module, etc., where a PID calculation program is built in the PLC module, and the PLC module may adjust the rotation speed of the negative pressure pump 21, so that the temperature of the refrigerant contained in the inner cavity of the refrigeration relay device 1 may be accurately adjusted. Alternatively, the PLC module incorporates a pressure relief program that actuates a vent valve on the container assembly 11 to vent and relieve pressure when the pressure in the interior cavity rises to a predetermined pressure. The positioning module is used for acquiring positioning information. The sensor module may include, for example, a thermocouple temperature sensor, a pressure sensor, a level gauge, etc., for acquiring a temperature of the refrigerant contained in the inner chamber, a pressure of the refrigerant, and a level of the refrigerant, respectively. The transmission module can be used for being connected with the negative pressure vaporizing device 2 and the parameter prompting device 4 in a communication way. The transmission module may comprise, for example, a wireless module and/or a bluetooth module. Further, the transmission module may be further used to communicate with a mobile terminal (such as a mobile phone), and in some embodiments, an operator may monitor at least one of the location information of the refrigeration relay 1, the temperature of the refrigerant contained in the inner cavity, the pressure of the refrigerant, and the liquid level of the refrigerant through the mobile terminal, or interact with the negative pressure vaporizing device 2 through the mobile terminal to input a predetermined temperature parameter.

The following describes the steps of using the refrigeration system provided in this embodiment, taking liquid nitrogen as a refrigerant:

1. the preparation process of supercooled liquid nitrogen comprises the following steps:

the refrigeration transfer device 1 is connected with the fourth housing 25 of the negative pressure vaporization device 2 according to the assembly position of the first housing 115, and the blind plug of the first connecting component 13 and the blind plug of the second connecting component 22 are used for assisting in positioning, so that the power supply of the negative pressure vaporization device 2 is connected.

The cover body component 12 of the refrigeration transfer device 1 is opened, liquid nitrogen is added into the liner 111 according to the requirement, and the liquid nitrogen can be injected by matching with a matched automatic liquid supplementing device. After filling with liquid nitrogen is completed, the cap assembly 12 is closed, and the first housing 115 and the second housing 122 are snapped on to complete the seal.

And inputting a preset temperature parameter on the interaction device 3, wherein the default value of the target temperature is-210 ℃, clicking a confirmation start key, operating the negative pressure pump 21 and the empty wet vaporizer 23, monitoring the temperature, the pressure or the liquid level of liquid nitrogen in the inner container 111 in real time through a display screen of the interaction device 3 and the parameter prompting device 4, and starting an automatic liquid supplementing device to supplement liquid in the inner container 111 when the liquid level is lower than a preset minimum liquid level value so as to ensure the liquid level of the liquid nitrogen. When the temperature of the supercooled liquid nitrogen reaches the target temperature, the rotating speed of the negative pressure pump 21 is regulated by a PID calculation program built in the PLC module so as to keep the temperature constant, and the preparation of the supercooled liquid nitrogen is completed.

2. Freezing process of biological tissue:

after the preparation of the supercooled liquid nitrogen is completed, the cover body component 12 of the refrigeration transfer device 1 is opened, a single freezing storage tube can be placed in the freezing storage tube for freezing, a storage box comprising a plurality of freezing storage tubes can also be placed in the freezing storage tube for freezing, then the cover body component 12 is closed, the buckle of the first shell 115 and the second shell 122 is buckled, and sealing is completed.

Optionally, the freezing transfer device 1 is matched with vision code scanning identification, can be matched with two-dimensional code information storage of a freezing storage tube, and is convenient to find and monitor. In the process, the temperature, pressure or liquid level of the liquid nitrogen in the liner 111 can be monitored in real time through the parameter prompt device 4 or the mobile terminal. It will be appreciated that the freezing process of the biological tissue may be performed after the freezing relay device 1 is separated from the negative pressure vaporizing device 2, or may be performed when the freezing relay device 1 is connected to the negative pressure vaporizing device 2, but before the cover assembly 12 is opened, the negative pressure pump 21 of the negative pressure vaporizing device 2 should be stopped, so that the pressure in the inner cavity is increased to be approximately equal to the external atmospheric pressure.

3. The transfer process comprises the following steps:

the process refrigeration transfer device 1 is separated from the negative pressure vaporization device 2, and after the freezing storage of biological tissues is finished, the process refrigeration transfer device 1 can be carried by matching with a matched AGV composite robot, and can also be operated by operators as required. In the transfer process, the mobile terminal can monitor the positioning information of the freezing transfer device 1, the temperature, the pressure and the liquid level of liquid nitrogen in real time, so that the transfer safety of biological tissues is ensured. If the pressure in the interior cavity rises to a predetermined pressure during the transfer process, the discharge valve on the container assembly 11 is vented to relieve pressure to ensure storage safety.

In summary, the refrigeration system provided by the present invention includes: a refrigeration transfer device and a negative pressure vaporization device; the refrigeration transfer device is detachably connected with the negative pressure vaporization device; the refrigeration transfer device is provided with an inner cavity for accommodating a refrigerant; when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporizing device is used for pumping negative pressure to the inner cavity so that the temperature of the refrigerant contained in the inner cavity is lower than the boiling point temperature of the refrigerant under the external atmospheric pressure.

So configured, the negative pressure is pumped to the inner cavity of the refrigeration transfer device through the negative pressure vaporization device, so that the boiling point of the refrigerant is reduced, the temperature of the refrigerant accommodated in the inner cavity of the refrigeration transfer device is reduced, and the cooling rate of biological tissues is improved. Therefore, on one hand, the vitrification of the cryoprotectant is facilitated, so that the concentration of the cryoprotectant can be reduced, the refrigeration toxicity and damage are reduced, and the quality of the refrigerated biological tissue is improved. On the other hand, the limitation of the size of the preserved biological tissue is reduced, the biological tissue with larger size can be preserved, and the preservation range is wider. In the other aspect, the freezing transfer device and the negative pressure vaporization device are separable, and when the freezing transfer device and the negative pressure vaporization device are combined, the negative pressure vaporization device can pump negative pressure to the inner cavity of the freezing transfer device according to the required pressure, so that the accurate control of the preservation temperature is realized, and the whole freezing system can be used as a long-term storage system for vitrification freezing storage; when the freezing transfer device is separated from the negative pressure vaporization device, the low-temperature refrigerant contained in the freezing transfer device can keep low temperature for a certain time, and can be transferred by biological tissues.

It should be noted that the above embodiments may be combined with each other. The above description is only illustrative of the preferred embodiments of the present invention and is not intended to limit the scope of the present invention, and any alterations and modifications made by those skilled in the art based on the above disclosure shall fall within the scope of the appended claims.

Claims (12)

1. A refrigeration system, comprising: a refrigeration transfer device and a negative pressure vaporization device; the refrigeration transfer device is detachably connected with the negative pressure vaporization device; the refrigeration transfer device is provided with an inner cavity for accommodating a refrigerant;

when the refrigeration transfer device is connected with the negative pressure vaporization device, the inner cavity is communicated with the negative pressure vaporization device; the negative pressure vaporization device is used for pumping negative pressure to the inner cavity.

2. The refrigeration system of claim 1, wherein the refrigeration relay apparatus comprises a container assembly and a cover assembly, the cover assembly being openably and closably connected to the container assembly; when the cover assembly is connected with the container assembly, the inner cavity is formed in a sealing mode.

3. The refrigeration system of claim 2, wherein the refrigeration relay apparatus further comprises a first connection assembly connected to the container assembly and in communication with the interior cavity, the movable end of the first connection assembly being configured to connect to the negative pressure vaporizing device.

4. A refrigeration system as set forth in claim 3 wherein said first connection assembly has a shut-off valve;

when the refrigeration transfer device is connected with the negative pressure vaporization device, the stop valve is connected;

and when the refrigeration transfer device is separated from the negative pressure vaporization device, the stop valve is closed.

5. The refrigeration system of claim 2, wherein the refrigeration relay apparatus further comprises a drain valve connected to the container assembly and in communication with the interior cavity;

when the refrigeration transfer device is separated from the negative pressure vaporization device and the pressure in the inner cavity does not exceed the preset pressure, the discharge valve is closed;

when the refrigeration transfer device is separated from the negative pressure vaporization device and the pressure in the inner cavity exceeds the preset pressure, the discharge valve is conducted, the inner cavity is depressurized, and the discharge valve is closed until the pressure in the inner cavity does not exceed the preset pressure; wherein the predetermined pressure is not less than the external atmospheric pressure.

6. The refrigeration system of claim 2, wherein the container assembly comprises: the refrigerator comprises an inner container, a first heat preservation sleeve and a supercooler; the cover assembly includes: a subcooler seal cover;

the first heat preservation sleeve is sleeved outside the inner container; the first heat preservation sleeve and the liner are contained in the subcooler together; the subcooler sealing cover is connected with the subcooler in a sealing way in an openable and closable manner.

7. The refrigeration system of claim 6, wherein the container assembly further comprises a shock pad located at a bottom outside the liner, respectively abutting the liner and the subcooler.

8. The refrigeration system of claim 6, wherein the container assembly further comprises: the first shell and the second heat preservation sleeve; the cover assembly further includes: the second shell and the third heat preservation sleeve; the first shell is used for being connected with the second shell in an adaptive manner;

the second heat preservation sleeve is sleeved outside the subcooler; the second heat preservation sleeve and the subcooler are contained in the first shell together; the subcooler sealing cover is connected with the second shell through the third heat preservation sleeve.

9. The refrigeration system of claim 1, wherein the negative pressure vaporizing device comprises: a negative pressure pump and a second connecting component connected with the negative pressure pump; the negative pressure pump is communicated with the inner cavity through the second connecting component and is used for pumping negative pressure to the inner cavity.

10. The refrigeration system of claim 9, wherein the negative pressure vaporizing device further comprises: an empty wet vaporizer and/or a bacteriostatic filter; the air wet vaporizer and/or the antibacterial filter are arranged between the negative pressure pump and the second connecting component.

11. The refrigeration system of claim 9, wherein the negative pressure vaporizing device further comprises: a fourth housing; the negative pressure pump and the second connecting component are accommodated in the fourth shell; the fourth shell is connected with the first shell of the freezing transfer device in an adaptive mode.

12. The refrigeration system of claim 1, wherein the refrigeration system further comprises: interaction means and/or parameter prompting means;

the interaction device is arranged on the negative pressure vaporization device; the interaction device is used for interactively inputting preset temperature parameters, and the negative pressure vaporization device pumps negative pressure to the inner cavity according to the preset temperature parameters so as to keep the temperature of the refrigerant accommodated in the inner cavity within a temperature interval corresponding to the preset temperature parameters;

the parameter prompting device is arranged on the refrigeration transfer device; the parameter prompting device is used for acquiring and prompting at least one of positioning information of the freezing transfer device, the temperature of the refrigerant contained in the inner cavity, the pressure of the refrigerant and the liquid level of the refrigerant.