CN220317817U - Pressurizing device for in-vitro cell culture - Google Patents
Pressurizing device for in-vitro cell culture Download PDFInfo
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- CN220317817U CN220317817U CN202321799200.9U CN202321799200U CN220317817U CN 220317817 U CN220317817 U CN 220317817U CN 202321799200 U CN202321799200 U CN 202321799200U CN 220317817 U CN220317817 U CN 220317817U
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- operation box
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- box body
- constant temperature
- cultivateing
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- 238000012606 in vitro cell culture Methods 0.000 title claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims description 30
- 238000001914 filtration Methods 0.000 claims description 23
- 238000009434 installation Methods 0.000 claims description 17
- 238000007789 sealing Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
- 239000007789 gas Substances 0.000 description 42
- 238000000338 in vitro Methods 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The utility model discloses a pressurizing device for in-vitro cell culture, which comprises a culture operation box body, wherein a support frame is arranged at the bottom end of the culture operation box body, a gas pressurizing structure is arranged in the support frame, the gas pressurizing structure comprises a gas storage tank, the gas storage tank is arranged in the support frame, one side of the gas storage tank is connected with a second conveying pipe, one side of the second conveying pipe is connected with a booster pump, and the top end of the booster pump is connected with a first conveying pipe.
Description
Technical Field
The utility model relates to the technical field of pressurizing devices, in particular to a pressurizing device for in-vitro cell culture.
Background
In vitro cell culture refers to in vitro simulation of in vivo environment, so that cells can grow, the cell culture is also called a cell cloning technology, the cell culture technology can be used for obtaining a large number of cells through a large number of culture of one cell into simple single cells or few differentiated multicells, and the cell culture can be used for researching signal transduction of the cells, anabolism of the cells, growth and proliferation of the cells and the like;
in the actual in-vitro cell culture process, the pressure needs to be changed to stimulate the cells, so that the in-vitro cells in the culture operation box body are pressurized by the pressurizing device, the in-vitro cell culture pressurizing device is needed, different gases can be arranged on the gas storage tank by arranging the gas pressurizing function and the gas storage tank, after being pressurized by the booster pump, the gas is conveyed into the culture operation box body through the first conveying pipe, and the cells are stimulated to promote the cell growth.
Disclosure of Invention
The present utility model is directed to a pressurizing device for in vitro cell culture, which solves the above-mentioned problems of the prior art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a supercharging device of external cell culture, includes the cultivation operation box, the support frame is installed to the bottom of cultivation operation box, the internally mounted of support frame has gas pressurization structure, gas pressurization structure includes the gas holding vessel, the inside at the support frame is installed to the gas holding vessel, one side of gas holding vessel is connected with the second conveyer pipe, one side of second conveyer pipe is connected with the booster pump, the top of booster pump is connected with first conveyer pipe, the air exit is installed to the order of cultivation operation box, high-efficient filtration is installed on the top of cultivation operation box, control panel is inlayed to the one end of cultivation operation box, sealing door is installed to the one end of cultivation operation box, the one end of sealing door is provided with the operation mouth, the internally mounted of cultivation operation box has the ultraviolet lamp that disinfects, the internally mounted of cultivation operation box has the constant temperature heating structure.
Preferably, the gas storage tank is communicated with the booster pump through a second conveying pipe, the booster pump is communicated with the culture operation box body through a first conveying pipe, and the first conveying pipe penetrates through one end of the culture operation box body.
Preferably, the efficient filtering structure comprises an air inlet, the bottom end of the air inlet is connected with a culture operation box body, a filtering module is arranged in the culture operation box body, a fan is arranged at the bottom end of the filtering module, and an air inlet grille is arranged in the culture operation box body.
Preferably, the air inlet is communicated with the filter module through the culture operation box body, the air inlet grille is communicated with the fan through the culture operation box body, and the filter module penetrates through the culture operation box body.
Preferably, the constant temperature heating structure comprises a constant temperature plate, the constant temperature plate is arranged in the culture operation box body, an installation box is arranged at the bottom end of the constant temperature plate, and a heating pipe is arranged in the installation box.
Preferably, the thermostatic board is connected with the installation box through the culture operation box body, the heating pipe runs through the inside of installation box, the heating pipe is connected with the thermostatic board through the installation box.
The pressurizing device for in-vitro cell culture provided by the utility model has the beneficial effects that: not only realizing the gas pressurizing function and the high-efficiency filtering function, but also realizing the constant-temperature heating function;
(1) Through setting up the gas pressurization structure, set up its bottom at the support frame, the gas that will need is carried to the gas holding vessel inside during the use, through carrying the gas in the gas holding vessel to the booster pump through the second conveyer pipe, carry out the pressure boost to the gas through the booster pump, then carry the gas after the pressure boost to the inside cultivation operation panel of cultivation operation box through first conveyer pipe, carry out the pressure boost to the inside cultured in vitro cell of cultivation operation box, thereby realized the gas pressure boost function, the gas holding vessel inside can store different grade type gas, the convenience is to the in vitro cell pressure boost of cultivation;
(2) Through setting up high-efficient filtration, with its setting in the inside of cultivation operation box, open the air intake when using through, start through the inside fan of cultivation operation box, get into inside the cultivation operation box through the air intake with outside air through the fan, filter the air that gets into through the inside filtration module of cultivation operation box, then carry the air that filters into the inside operation space of cultivation operation box through the air inlet grille, thereby realized high-efficient filtration function, the inside gaseous external cell operation environment of cultivation of convenient cultivation operation box reaches the cultivation of convenient operation;
(3) Through setting up constant temperature heating structure, with its inside that sets up at the cultivation operation box, start the inside heating pipe of mounting box through the control panel of cultivation operation box one end during the use, through on giving the inside thermostatic board of cultivation operation box with the heating pipe transmission, carry out constant temperature heating through thermostatic board heat to the inside cultured external cell of cultivation operation box to realized constant temperature heating function, help external cell growth.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic elevational view of the present utility model;
FIG. 2 is a schematic cross-sectional view of a high efficiency filter structure according to the present utility model;
FIG. 3 is a schematic cross-sectional view of a gas pressurizing structure according to the present utility model;
fig. 4 is a schematic view of a partial sectional structure of a constant temperature heating structure according to the present utility model.
In the figure: 1. an air outlet; 2. a high-efficiency filtering structure; 201. an air inlet; 202. a filtration module; 203. a fan; 204. an air intake grille; 3. a control panel; 4. a culture operation box body; 5. sealing the door; 6. an operation port; 7. a support frame; 8. a gas pressurizing structure; 801. a gas storage tank; 802. a booster pump; 803. a first delivery tube; 804. a second delivery tube; 9. a germicidal ultraviolet lamp; 10. a constant temperature heating structure; 1001. a thermostatic plate; 1002. heating pipes; 1003. and (3) a mounting box.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. All other embodiments, which can be made by one of ordinary skill in the art without inventive faculty, are intended to be within the scope of the present utility model, based on the embodiments of the present utility model.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1-4, the present utility model provides a technical solution: the pressurizing device for in-vitro cell culture comprises a culture operation box body 4, wherein a support frame 7 is arranged at the bottom end of the culture operation box body 4, a gas pressurizing structure 8 is arranged in the support frame 7, the gas pressurizing structure 8 comprises a gas storage tank 801, the gas storage tank 801 is arranged in the support frame 7, one side of the gas storage tank 801 is connected with a second conveying pipe 804, one side of the second conveying pipe 804 is connected with a booster pump 802, and the top end of the booster pump 802 is connected with a first conveying pipe 803;
the gas storage tank 801 is communicated with the booster pump 802 through a second conveying pipe 804, the booster pump 802 is communicated with the culture operation box body 4 through a first conveying pipe 803, and the first conveying pipe 803 penetrates through one end of the culture operation box body 4;
specifically, as shown in fig. 1 and 3, by providing the gas pressurizing structure 8, when in use, required gas is conveyed into the gas storage tank 801, the gas in the gas storage tank 801 is conveyed to the booster pump 802 through the second conveying pipe 804, the gas is pressurized through the booster pump 802, and then the pressurized gas is conveyed into the culture operation table in the culture operation box 4 through the first conveying pipe 803, so that the cultured in-vitro cells in the culture operation box 4 are pressurized, and the gas pressurizing function is realized;
an air outlet 1 is arranged on an order of the culture operation box body 4, a high-efficiency filtering structure 2 is arranged at the top end of the culture operation box body 4, the high-efficiency filtering structure 2 comprises an air inlet 201, the bottom end of the air inlet 201 is connected with the culture operation box body 4, a filtering module 202 is arranged in the culture operation box body 4, a fan 203 is arranged at the bottom end of the filtering module 202, and an air inlet grille 204 is arranged in the culture operation box body 4;
the air inlet 201 is communicated with the filter module 202 through the culture operation box 4, the air inlet grille 204 is communicated with the fan 203 through the culture operation box 4, and the filter module 202 penetrates through the interior of the culture operation box 4;
specifically, as shown in fig. 1 and 2, by providing the efficient filtering structure 2, when the air inlet 201 is opened during use, the air inlet 201 is started by the fan 203 inside the culture operation box 4, external air enters the culture operation box 4 through the air inlet 201 by the fan 203, the air entering the culture operation box 4 is filtered by the filtering module 202 inside the culture operation box 4, and then the filtered air is conveyed into the operation space inside the culture operation box 4 through the air inlet grille 204, so that the efficient filtering function is realized;
one end of the culture operation box body 4 is inlaid with a control panel 3, one end of the culture operation box body 4 is provided with a sealing door 5, one end of the sealing door 5 is provided with an operation port 6, the interior of the culture operation box body 4 is provided with a sterilizing ultraviolet lamp 9, the interior of the culture operation box body 4 is provided with a constant temperature heating structure 10, the constant temperature heating structure 10 comprises a constant temperature plate 1001, the constant temperature plate 1001 is arranged in the culture operation box body 4, the bottom end of the constant temperature plate 1001 is provided with an installation box 1003, and the interior of the installation box 1003 is provided with a heating pipe 1002;
the thermostat 1001 is connected with the installation box 1003 through the culture operation box 4, the heating pipe 1002 penetrates through the inside of the installation box 1003, and the heating pipe 1002 is connected with the thermostat 1001 through the installation box 1003;
specifically, as shown in fig. 1 and 4, by providing the constant temperature heating structure 10, the heating pipe 1002 inside the installation box 1003 is started by the control panel 3 at one end of the culturing operation box 4 when in use, by transferring the heating pipe 1002 to the constant temperature plate 1001 inside the culturing operation box 4, the cells outside the culturing body inside the culturing operation box 4 are heated at constant temperature by the heat of the constant temperature plate 1001, thereby realizing the constant temperature heating function.
Working principle: in the practical use process, firstly, the air inlet 201 is opened, then the fan 203 is started through the control panel 3 during the use, external air enters the filter module 202 in the culture operation box 4 through the air inlet 201 at the top end of the culture operation box 4, the air is filtered through the filter module 202 and then enters the operation box in the culture operation box 4 through the air inlet grid 204 in the culture operation box 4, the culture operation box 4 is sealed through the sealing door 5, the sterilizing ultraviolet lamp 9 is started to sterilize the inside and the air, then the cultured extracorporeal cells are put into the culture operation box 4, the cultured extracorporeal cells are operated through the operation opening 6 of one section of the sealing door 5, the heating pipe 1002 in the installation box 1003 is started through the control panel 3, the cultured extracorporeal cells can be grown in a proper environment by the constant temperature heating of the heating pipe 1002 through the constant temperature plate 1001, finally, the gas can be conveyed into the gas storage tank 801, the gas storage tank 802 is conveyed into the culture box through the second operation pipe 802, and the pressurizing pump is conveyed into the culture box 803 through the second operation pipe 802, and the pressurizing pump is finally, the cultured extracorporeal cells are conveyed into the culture box 4 through the second operation pipe 802, and the pressurizing pump is used for pressurizing the culture device is completed.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
The apparatus embodiments described above are merely illustrative, wherein the elements illustrated as separate elements may or may not be physically separate, and the elements shown as elements may or may not be physical elements, may be located in one place, or may be distributed over a plurality of network elements. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment. Those of ordinary skill in the art will understand and implement the present utility model without undue burden.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.
Claims (6)
1. The utility model provides a pressurizing device of in vitro cell culture, includes cultivation operation box (4), its characterized in that: support frame (7) are installed to the bottom of cultivateing operation box (4), internally mounted of support frame (7) has gaseous pressurization structure (8), gaseous pressurization structure (8) are including gas holding vessel (801), the inside at support frame (7) is installed to gas holding vessel (801), one side of gas holding vessel (801) is connected with second conveyer pipe (804), one side of second conveyer pipe (804) is connected with booster pump (802), the top of booster pump (802) is connected with first conveyer pipe (803), air exit (1) are installed to the order of cultivateing operation box (4), high-efficient filtration (2) are installed on the top of cultivateing operation box (4), control panel (3) are inlayed to the one end of cultivateing operation box (4), sealing door (5) are installed to the one end of cultivateing operation box (4), the one end of sealing door (5) is provided with operation mouth (6), the internally mounted of cultivateing operation box (4) has ultraviolet lamp (9), the inside mounting of cultivateing operation box (4) has the heat sterilization structure (10).
2. The pressurization device for in vitro cell culture according to claim 1, wherein: the gas storage tank (801) is communicated with the booster pump (802) through the second conveying pipe (804), the booster pump (802) is communicated with the culture operation box body (4) through the first conveying pipe (803), and the first conveying pipe (803) penetrates through one end of the culture operation box body (4).
3. The pressurization device for in vitro cell culture according to claim 1, wherein: the efficient filtering structure (2) comprises an air inlet (201), the bottom end of the air inlet (201) is connected with a culture operation box body (4), a filtering module (202) is installed in the culture operation box body (4), a fan (203) is installed at the bottom end of the filtering module (202), and an air inlet grille (204) is arranged in the culture operation box body (4).
4. A pressurizing device for in vitro cell culture according to claim 3, wherein: the air inlet (201) is communicated with the filtering module (202) through the culture operation box body (4), the air inlet grille (204) is communicated with the fan (203) through the culture operation box body (4), and the filtering module (202) penetrates through the culture operation box body (4).
5. The pressurization device for in vitro cell culture according to claim 1, wherein: the constant temperature heating structure (10) comprises a constant temperature plate (1001), wherein the constant temperature plate (1001) is arranged in the culture operation box body (4), an installation box (1003) is arranged at the bottom end of the constant temperature plate (1001), and a heating pipe (1002) is arranged in the installation box (1003).
6. The apparatus of claim 5, wherein the apparatus comprises: the constant temperature plate (1001) is connected with the installation box (1003) through the culture operation box body (4), the heating pipe (1002) penetrates through the inside of the installation box (1003), and the heating pipe (1002) is connected with the constant temperature plate (1001) through the installation box (1003).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321799200.9U CN220317817U (en) | 2023-07-10 | 2023-07-10 | Pressurizing device for in-vitro cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321799200.9U CN220317817U (en) | 2023-07-10 | 2023-07-10 | Pressurizing device for in-vitro cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220317817U true CN220317817U (en) | 2024-01-09 |
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ID=89425294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321799200.9U Active CN220317817U (en) | 2023-07-10 | 2023-07-10 | Pressurizing device for in-vitro cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220317817U (en) |
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2023
- 2023-07-10 CN CN202321799200.9U patent/CN220317817U/en active Active
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